四氢阿曼托黄素抗氧化(自由基清除)活性及机理

四氢阿曼托黄素抗氧化（自由基清除）活性及机理2017.11

[参考文献]Xican Li, Weijuan Han, Wengqiong Mai, Li Wang. Antioxidant Activity and Mechanism of Tetrahydroamentoflavone in vitro. Natural Product Communications, 2013, 8,787-789.

[简介]四氢阿曼托黄素（Tetrahydroamentoflavone）是一种典型的双黄酮，存在于许多植物中。其结构式如下：

四氢阿曼托黄素的结构式与编号

从其分子模型可以看出，四氢阿曼托黄素的四个环并不是在同一个平面上。

四氢阿曼托黄素的分子模型

四氢阿曼托黄素可以清除超氧自由基（•O2-），清除DPPH•自由基，清除ABTS•+自由基，能力Cu2+还原。此外，还可以与Fe2+、Cu2+络合（IC50值见表1）。这表明，四氢阿曼托黄素具有抗氧化活性。它可能通过金属络合和自由基清除作用发挥其抗氧化活性。自由基清除作用其可能通过提供氢原子（H·）和电子（e）来清除自由基实现。

Table 1 The IC50values of tetrahydroamentoflavone (μg/mL)

DPPH ABTS

Reducing power

·O2-·OH

Metal chelating Fe3+Cu2+Fe2+Cu2+

Tetrahydroamentoflavone 1.88±0.25a 1.88±0.25a 1.88±0.25a 1.88±0.25a 1.88±0.25a 1.88±0.25a 1.88±0.25a 1.88±0.25a Trolox 1.88±0.25b 1.88±0.25b 1.88±0.25b 1.88±0.25b 1.88±0.25b 1.88±0.25b 1.88±0.25b 1.88±0.25b BHA 1.88±0.25c 1.88±0.25c 1.88±0.25c 1.88±0.25c 1.88±0.25c 1.88±0.25c 1.88±0.25c 1.88±0.25c IC50,Trolox/ IC50,THA

Trolox, BHA, sodium citratewere used as positive controls. IC50 value is defined as the concentration of 50%

inhibition percentages and calculated by linear regression analysis and expressed as Mean±SD (n=3). The linear

regression was analyzed by Origin 6.0 professional software. Means values with different superscripts in the same

column are significantly different (p<0.05).Their dose response curveswere shown in Supplementary Material.