壳寡糖通便功能的研究

食品研究与开发

F ood Research And Development

圆园18年9月第39卷第17期

DOI：10.3969/j.issn.1005-6521.2018.17.031

壳寡糖通便功能的研究

梁宇轩，欧阳冬梅，王达，陈咏春，刘焕，贺永健，庄新栋，黄日明，孙远明*，王弘*

（华南农业大学食品学院，广东省食品质量与安全重点实验室，广东广州510642）

摘要：研究壳寡糖对小鼠通便功能。将昆明雄性小鼠按体重随机分成空白对照组、模型对照组和3个剂量组。剂量组分别经口给予低、中、高剂量（99.2、198.4、396.8mg/（kg·d））壳寡糖溶液；空白对照组和模型对照组给予蒸馏水，干预时间为7d。用盐酸洛哌丁胺造小鼠便秘模型。观察各组小鼠的小肠墨汁推进率、首粒排黑便时间、6h黑便粒数和6h黑便重量以及粪便含水量。结果表明：与模型对照组比，壳寡糖中剂量组小肠墨汁推进率和6h黑便重量显著增加（p<0.05）；壳寡糖高剂量组小肠墨汁推进率极显著增加（p<0.01）、首粒排黑便时间极显著缩短（p<0.01）、6h黑便重量与6h黑便粒数显著增加（p<0.05）；在粪便含水量上，各剂量组同空白对照组、模型对照组比无显著性差异。壳寡糖能有效增强小鼠肠道蠕动并促进排便，具有通便功能。

关键词：壳寡糖；低聚糖；通便；小鼠；便秘

Study on the Laxative Effect of Chitooligosaccharide

LIANG Yu-xuan，OUYANG Dong-mei，WANG Da，CHEN Yong-chun，LIU Huan，HE Yong-jian，ZHUANG

Xin-dong，HUANG Ri-ming，SUN Yuan-ming*，WANG Hong*

（College of Food Science，South China Agricultural University，Guangdong Provincial Key Laboratory of Food

Quality and Safety，Guangzhou510642，Guangdong，China）

Abstract：To investigate the laxative effect of chitooligosaccharide in mice.Kunming male mice were randomly divided into blank control group，model control group and three dose groups.Dose groups were given low，medium and high doses of chitooligosaccharide solution[99.2，198.4，396.8mg/（kg·d）]，while the blank control group and model control group were given distilled water，with7day intervention.Mouse model was made with loperamide hydrochloride.Ink propulsion rate in the small intestine，the time of defecation of the first black feces，the defecation amount and weights in six hours，and the moisture content of stool were observed in each group.Results showed that：compared with the model control group，the medium dose of chitooligosaccharide could increase intestinal propulsive rate and the dejection weights in six hours significantly（p<0.05）；the high dose of chitooligosaccharide could significantly increase intestinal propulsive rate（p<0.01），the dejection amount and weights in six hours（p<0.05），and significantly shorten the time of defecation of the first black feces （p<0.01）.There was no significant difference between the dose group and the blank control group and model control group in the water content of feces.The chitooligosaccharide significantly improves mice's defecation and intestinal peristalsis with laxative effect.

Key words：chitooligosaccharide；oligosaccharide；laxative effect；mice；constipation

引文格式：

梁宇轩，欧阳冬梅，王达，等.壳寡糖通便功能的研究[J].食品研究与开发，2018，39（17）：173-178

LIANG Yuxuan，OUYANG Dongmei，WANG Da，et al.Study on the Laxative Effect of Chitooligosaccharide[J].Food Research and Development，2018，39（17）：173-178

基金项目：广东省科技计划项目（2017A020217002）

作者简介：梁宇轩（1992—），男（回），硕士研究生，研究方向：食品营养与安全。

*通信作者：孙远明（1956—），男，博士，教授，研究方向：食品质量与安全；王弘（1973—），女，博士，教授，研究方向：食品营养与安全。营养保健173