基于网络药理学的沙棘总黄酮治疗心肌缺血的作用机制研究_魏志成

［收稿日期］2016-11-08

［基金项目］国家自然科学基金面上项目（81473428）［通信作者］

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张艺，研究员，博士生导师，研究方向为中药质量标准及民族医药研究，

Tel ：（028）61800274，E-mail ：9006zmy@ ［作者简介］魏志成，硕士研究生，主要从事中药药效和毒理学研究，

E-mail ：weizhicheng546@ 基于网络药理学的沙棘总黄酮治疗心肌

缺血的作用机制研究

魏志成，童东，杨娟，赵可惠，孟宪丽，张艺

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（成都中医药大学，四川成都611137）

［摘要］该研究采用网络药理学虚拟筛选方法，旨在进一步研究沙棘总黄酮（total flavonoids of Hippophae rhamnoides ，TFH ）治疗心肌缺血的活性成分和作用机制。首先通过TCMSP 数据库和PubChem 数据库，结合口服利用度和类药性分析，筛选出沙棘化合物中的黄酮类化合物。筛选得到的7个化合物再通过ChemMapper 服务器进行靶点的预测分析。将得到的潜在靶点导入MAS 3.0数据库，结合KEGG 数据库进行靶点分析和通路分析。最后使用Cystoscope 3.3.0软件绘制“化合物-靶点-作用通路”网络图。虚拟实验预测得到了68个潜在靶点和60条信号通路，其中有31个靶点和23条通路直接或间接与心肌缺血有关。结果表明，TFH 主要通过对钙离子信号通路（calcium signaling pathway ）、血管内皮生长因子信号通路（VEGF signaling pathway ）和缝隙连接信号通路（gap junction ）等信号通路的调节发挥协同作用，且与文献报道吻合；提示其可能通过参与调节血小板聚集、脂质代谢、炎症反应等过程，增强心功能和对血管内皮细胞保护作用，从而发挥抗心肌缺血的作用。

［关键词］沙棘；沙棘总黄酮；网络药理学；心肌缺血；机制研究

Action mechanism of total flavonoids of Hippophae rhamnoides in

treatment of myocardial ischemia based on network pharmacology

WEI Zhi-cheng ，TONG Dong ，YANG Juan ，ZHAO Ke-hui ，MENG Xian-li ，ZHANG Yi *

（Chengdu University of Traditional Chinese Medicine ，Chengdu 611137，China ）

［Abstract ］In this study ，a network pharmacological screening method was adopted to further study the active ingredients and action mechanism of total flavonoids of Hippophae rhamnoides （TFH ）for the treatment of myocardial ischemia.Firstly TCMSP database and PubChem database were searched ，and then the data were combined with oral bioavailability and drug analysis to screen flavonoids of H .rhamnoides compounds.Then predictive analysis was conducted for the 7screened compounds by ChemMapper server.The obtained potential targets were imported into MAS 3.0.Database ，and KEGG database was also used for targets analysis and pathway analysis.Finally Cystoscope 3.3.0software was used to draw "compounds-targets-pathway"network diagram.Virtual experiments predicted 68potential targets and 60signaling pathways ，and 31targets and 23pathways of them were directly or indirectly associated with myocar-dial ischemia.The results showed that TFH played a synergistic rolemainly through the regulation of calcium signaling pathway ，VEGF signaling pathway and gap junction signaling pathway ，which was consistent with literature reports.These results indicated that it can enhance heart function ，protect vascular endothelial cells ，and fight against myocardial ischemia probably by regulating platelet aggre-gation ，lipid metabolism ，inflammation and other processes.

［Key words ］Hippophae rhamnoides ；TFH ；network pharmacology ；myocardial ischemia ；mechanism

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8321·DOI:10.19540/ki.cjcmm.20161222.077