分子生物学实验-Molecular Biology Practical
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• Lab 3 plasmid DNA extraction and Qualitative and Quantitative Analysis
• Lab 4 Total RNA extraction, Reverse transcription and RT-PCR
4.Correctly usethe related experimental instruments such asmicro-sample feeder, high pressure steam sterilizer, constant temperature shaking table, super clean workbench, high speed refrigerated centrifuge, ice maker, PCR instrument, horizontal electrophoresis apparatus, gel imaging systemetc.beskilled in aseptic operation technologyand developed withindependent operation ability and teamwork cooperation spirit.
2.Master the process and basic steps of the experimental technology of bacterial plasmid DNA artificial recombination, as well asthe main experimental techniques of the DNA artificial recombination of animal and plant cells,design the experimentwiththese techniques,observe and analyze the experimental phenomena.
பைடு நூலகம்Pre-requisites
Molecular Biology
Intended Learning Outcomes
The anticipated knowledge, skills and attitude to be developed by the studentsare:
1.Understand the technical system and development status of molecular biology experiment,toenhancethe subjective desire to deeply understand the theoretical knowledge and technical principles of molecular biology, more activelytoexplorerelevant scientific problems in the field of molecular biology and to solve practical problemsusing existing technologies.
Learning Schedule
• Lab 1 Brief introduction, Genomic DNA extraction; PCR amplification
• Lab 2 Construction of recombinant DNA, transformation and selection
3.Independentlyconductgenomic DNA extraction, Plasmid Extraction, total RNA extraction, PCR amplification, RT-PCR amplification, agarose gel electrophoresis;observe and analyze the experimental phenomena;Independentlyconductthe experimental operation of transformingtarget genesinto the competentE.colicellsviaplasmid DNA.
Syllabus ofBiotechnologyat Haide College
Molecular Biology Practical
Description
Molecular Biology Laboratory is a system of molecular biology experiment technology based on biochemistry experiment and microbiology experiment, which is a modern biological technology for research and application of life science. This course is aimed at senior students majoring in biology and biotechnology. It mainly introduces the basic operational techniques and experimental means of molecular biology, including Principles and procedures of experimental projects such as genomic DNA extraction and identification, plasmid DNA extraction and identification, total RNA extraction and identification, PCR amplification, restriction endonuclease digestion, processofmolecular cloning and construction of gene library. Through the course study and operation practice, students are required to master the basic principles and operation skills of molecular biology experiments, and to be able to analyze and solve practical problems in related fields of molecular biologywiththese techniques.
• Lab 4 Total RNA extraction, Reverse transcription and RT-PCR
4.Correctly usethe related experimental instruments such asmicro-sample feeder, high pressure steam sterilizer, constant temperature shaking table, super clean workbench, high speed refrigerated centrifuge, ice maker, PCR instrument, horizontal electrophoresis apparatus, gel imaging systemetc.beskilled in aseptic operation technologyand developed withindependent operation ability and teamwork cooperation spirit.
2.Master the process and basic steps of the experimental technology of bacterial plasmid DNA artificial recombination, as well asthe main experimental techniques of the DNA artificial recombination of animal and plant cells,design the experimentwiththese techniques,observe and analyze the experimental phenomena.
பைடு நூலகம்Pre-requisites
Molecular Biology
Intended Learning Outcomes
The anticipated knowledge, skills and attitude to be developed by the studentsare:
1.Understand the technical system and development status of molecular biology experiment,toenhancethe subjective desire to deeply understand the theoretical knowledge and technical principles of molecular biology, more activelytoexplorerelevant scientific problems in the field of molecular biology and to solve practical problemsusing existing technologies.
Learning Schedule
• Lab 1 Brief introduction, Genomic DNA extraction; PCR amplification
• Lab 2 Construction of recombinant DNA, transformation and selection
3.Independentlyconductgenomic DNA extraction, Plasmid Extraction, total RNA extraction, PCR amplification, RT-PCR amplification, agarose gel electrophoresis;observe and analyze the experimental phenomena;Independentlyconductthe experimental operation of transformingtarget genesinto the competentE.colicellsviaplasmid DNA.
Syllabus ofBiotechnologyat Haide College
Molecular Biology Practical
Description
Molecular Biology Laboratory is a system of molecular biology experiment technology based on biochemistry experiment and microbiology experiment, which is a modern biological technology for research and application of life science. This course is aimed at senior students majoring in biology and biotechnology. It mainly introduces the basic operational techniques and experimental means of molecular biology, including Principles and procedures of experimental projects such as genomic DNA extraction and identification, plasmid DNA extraction and identification, total RNA extraction and identification, PCR amplification, restriction endonuclease digestion, processofmolecular cloning and construction of gene library. Through the course study and operation practice, students are required to master the basic principles and operation skills of molecular biology experiments, and to be able to analyze and solve practical problems in related fields of molecular biologywiththese techniques.