酶切位点保护性碱基
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CCTCGAGG CCCTCGAGGG CCGCTCGAGCGG
CCCCGGGG CCCCCGGGGG CCCCCCGGGGGG TCCCCCCGGGGGGA
0
0
>90
>90
75
>90
75
>90
0
0
10
25
10
75
0
0
25
75
50
>90
>90
>90
GTTTAAAC GGTTTAAACC GGGTTTAAACCC
0
0
0
0
10
75
0
0
>90
>90
>90
>90
0
0
25
50
0
0
50
75
0
0
0
0
0
0
0
0
75
>90
75
>90
0
0
10
25
10
50
切割率%
2 hr
20 hr
0
0
10
10
10
10
25
90
25
>90
10
>90
>90
>90
0
0
0
25
0
>90
0
0
0
25
酶 Acc I Afl III Asc I Ava I BamH I Bgl II BssH II BstE II BstX I Cla I
EcoR I Hae III
寡核苷酸序列
GGTCGACC CGGTCGACCG CCGGTCGACCGG
CACATGTG CCACATGTGG CCCACATGTGGG
GACGCGTC CGACGCGTCG
CCCATGGG CATGCCATGGCATG
CCATATGG CCCATATGGG CGCCATATGGCG GGGTTTCATATGAAACCC GGAATTCCATATGGAATTCC GGGAATTCCATATGGAATTCCC
GGCTAGCC CGGCTAGCCG CTAGCTAGCTAG
GGGT(A/T)ACCC
AACTGCAGAACCAATGCATTGG AAAACTGCAGCCAATGCATTGGAA CTGCAGAACCAATGCATTGGATGCAT
CATCGATG GATCGATC CCATCGATGG CCCATCGATGGG
GGAATTCC CGGAATTCCG CCGGAATTCCGG
50
>90
>90
>90
>90
>90
>90
>90
>90
>90
>90
TTGCGGCCGCAA
>90
>90
Hind III Kpn I Mlu I Nco I Nde I
Nhe I 酶 Not I
Nsi I Pac I Pme I
CAAGCTTG CCAAGCTTGG CCCAAGCTTGGG
GGGTACCC GGGGTACCCC CGGGGTACCCCG
GGGGCCCC AGCGGCCGCT
切割率%
2 hr 20 hr
0
0
0
0
0
0Leabharlann Baidu
0
0
>90
>90
>90
>90
>90
>90
>90
>90
>90
>90
50
>90
>90
>90
>90
>90
10
25
>90
>90
>90
>90
0
0
75
>90
25
>90
0
0
0
0
50
>90
0
10
0
0
25
50
25
>90
0
0
0
0
>90
>90
50
寡核苷酸序列
TTGCGGCCGCAA ATTTGCGGCCGCTTTA AAATATGCGGCCGCTATAAA ATAAGAATGCGGCCGCTAAACTAT AAGGAAAAAAGCGGCCGCAAAAGGAAAA
TGCATGCATGCA CCAATGCATTGGTTCTGCAGTT
TTAATTAA GTTAATTAAC CCTTAATTAAGG
10
>90
10
>90
0
50
0
50
Sph I
GGCATGCC CATGCATGCATG ACATGCATGCATGT
0
0
0
25
10
50
Stu I
AAGGCCTT GAAGGCCTTC AAAAGGCCTTTT
>90
>90
>90
>90
>90
>90
Xba I Xho I Xma I
CTCTAGAG GCTCTAGAGC TGCTCTAGAGCA CTAGTCTAGACTAG
>90
0
0
Pvu I
CCGATCGG ATCGATCGAT TCGCGATCGCGA
0
0
10
25
0
10
Sac I
CGAGCTCG
10
10
Sac II
GCCGCGGC TCCCCGCGGGGA
0
0
50
>90
Sal I
GTCGACGTCAAAAGGCCATAGCGGCCGC
0
0
GCGTCGACGTCTTGGCCATAGCGGCCGCG
0
50
AGCTTTGTTTAAACGGCGCGCCGG
75
>90
Pst I
GCTGCAGC TGCACTGCAGTGCA AACTGCAGAACCAATGCATTGG AAAACTGCAGCCAATGCATTGGAA CTGCAGAACCAATGCATTGGATGCAT
0
0
10
10
>90
>90
>90
GGCGCGCC AGGCGCGCCT TTGGCGCGCCAA
CCCCGGGG CCCCCGGGGG TCCCCCGGGGGA
CGGATCCG CGGGATCCCG CGCGGATCCGCG
CAGATCTG GAAGATCTTC GGAAGATCTTCC
GGCGCGCC AGGCGCGCCT TTGGCGCGCCAA
10
50
G
10
75
ACGCGTCGACGTCGGCCATAGCGGCCGCG
GAA
Sca I
GAGTACTC AAAAGTACTTTT
10
25
75
75
Sma I
CCCGGG CCCCGGGG CCCCCGGGGG TCCCCCGGGGGA
0
10
0
10
10
50
>90
>90
Spe I
GACTAGTC GGACTAGTCC CGGACTAGTCCG CTAGACTAGTCTAG
CCCCGGGG CCCCCGGGGG CCCCCCGGGGGG TCCCCCCGGGGGGA
0
0
>90
>90
75
>90
75
>90
0
0
10
25
10
75
0
0
25
75
50
>90
>90
>90
GTTTAAAC GGTTTAAACC GGGTTTAAACCC
0
0
0
0
10
75
0
0
>90
>90
>90
>90
0
0
25
50
0
0
50
75
0
0
0
0
0
0
0
0
75
>90
75
>90
0
0
10
25
10
50
切割率%
2 hr
20 hr
0
0
10
10
10
10
25
90
25
>90
10
>90
>90
>90
0
0
0
25
0
>90
0
0
0
25
酶 Acc I Afl III Asc I Ava I BamH I Bgl II BssH II BstE II BstX I Cla I
EcoR I Hae III
寡核苷酸序列
GGTCGACC CGGTCGACCG CCGGTCGACCGG
CACATGTG CCACATGTGG CCCACATGTGGG
GACGCGTC CGACGCGTCG
CCCATGGG CATGCCATGGCATG
CCATATGG CCCATATGGG CGCCATATGGCG GGGTTTCATATGAAACCC GGAATTCCATATGGAATTCC GGGAATTCCATATGGAATTCCC
GGCTAGCC CGGCTAGCCG CTAGCTAGCTAG
GGGT(A/T)ACCC
AACTGCAGAACCAATGCATTGG AAAACTGCAGCCAATGCATTGGAA CTGCAGAACCAATGCATTGGATGCAT
CATCGATG GATCGATC CCATCGATGG CCCATCGATGGG
GGAATTCC CGGAATTCCG CCGGAATTCCGG
50
>90
>90
>90
>90
>90
>90
>90
>90
>90
>90
TTGCGGCCGCAA
>90
>90
Hind III Kpn I Mlu I Nco I Nde I
Nhe I 酶 Not I
Nsi I Pac I Pme I
CAAGCTTG CCAAGCTTGG CCCAAGCTTGGG
GGGTACCC GGGGTACCCC CGGGGTACCCCG
GGGGCCCC AGCGGCCGCT
切割率%
2 hr 20 hr
0
0
0
0
0
0Leabharlann Baidu
0
0
>90
>90
>90
>90
>90
>90
>90
>90
>90
>90
50
>90
>90
>90
>90
>90
10
25
>90
>90
>90
>90
0
0
75
>90
25
>90
0
0
0
0
50
>90
0
10
0
0
25
50
25
>90
0
0
0
0
>90
>90
50
寡核苷酸序列
TTGCGGCCGCAA ATTTGCGGCCGCTTTA AAATATGCGGCCGCTATAAA ATAAGAATGCGGCCGCTAAACTAT AAGGAAAAAAGCGGCCGCAAAAGGAAAA
TGCATGCATGCA CCAATGCATTGGTTCTGCAGTT
TTAATTAA GTTAATTAAC CCTTAATTAAGG
10
>90
10
>90
0
50
0
50
Sph I
GGCATGCC CATGCATGCATG ACATGCATGCATGT
0
0
0
25
10
50
Stu I
AAGGCCTT GAAGGCCTTC AAAAGGCCTTTT
>90
>90
>90
>90
>90
>90
Xba I Xho I Xma I
CTCTAGAG GCTCTAGAGC TGCTCTAGAGCA CTAGTCTAGACTAG
>90
0
0
Pvu I
CCGATCGG ATCGATCGAT TCGCGATCGCGA
0
0
10
25
0
10
Sac I
CGAGCTCG
10
10
Sac II
GCCGCGGC TCCCCGCGGGGA
0
0
50
>90
Sal I
GTCGACGTCAAAAGGCCATAGCGGCCGC
0
0
GCGTCGACGTCTTGGCCATAGCGGCCGCG
0
50
AGCTTTGTTTAAACGGCGCGCCGG
75
>90
Pst I
GCTGCAGC TGCACTGCAGTGCA AACTGCAGAACCAATGCATTGG AAAACTGCAGCCAATGCATTGGAA CTGCAGAACCAATGCATTGGATGCAT
0
0
10
10
>90
>90
>90
GGCGCGCC AGGCGCGCCT TTGGCGCGCCAA
CCCCGGGG CCCCCGGGGG TCCCCCGGGGGA
CGGATCCG CGGGATCCCG CGCGGATCCGCG
CAGATCTG GAAGATCTTC GGAAGATCTTCC
GGCGCGCC AGGCGCGCCT TTGGCGCGCCAA
10
50
G
10
75
ACGCGTCGACGTCGGCCATAGCGGCCGCG
GAA
Sca I
GAGTACTC AAAAGTACTTTT
10
25
75
75
Sma I
CCCGGG CCCCGGGG CCCCCGGGGG TCCCCCGGGGGA
0
10
0
10
10
50
>90
>90
Spe I
GACTAGTC GGACTAGTCC CGGACTAGTCCG CTAGACTAGTCTAG