北师大细胞生物学a

§4. How to study cells 1. Structural levels and resolution power of observation instruments 2. Light microscopy 3. Electron microscopy 4. Cell sorting, cell culture and cell clone 5. The fractionation of a cell’s contents by differential centrifugation 6. Biochemical and Molecular biological technique 7. Analysis of cells by non-impairment detect techniques
proteins 4) Synthesis and targeting of peroxisomal
proteins 5) Lipid-anchored membrane proteins onto the
cytoplasmic leaflet
§8. Cytoplasmic membrane systems
细胞科学
Cell Science
蔡国平
§1 The Evolutionary History of Cell Biology in Brief
1. Discovery of cell and the evolution of
cell theory.
(~1850s)
2. Establishment of cytology- classic period
(1850-1900)
3. Experiment cytological period
(1900-1950)
4. Cell biology period
(1950-)
§2 The Position of the Cell in the Order of Life and Responsibilities of Cell Biology 1. The order of life and organisation of life 2. The position of the cell in the order of life 3. Responsibilities of cell biology
1. The dynamic nature of the endomembrane system 2. A few approaches to study of endomembranes 3 Structues and funcions of the major organelles of EMS
§7. Cytosol and the synthesis of proteins
1. Cytosol 2. Microtrabecular system 3. Ribosomes and proteins synthesis 4. Sorting and trans3. The general structure and multiplicity of the cell
1.Biogap 2. viruses, viroid and prion 3. Two fundamentally different classes
of cells 4.The three kingdoms of organisms
§5. The general structure and function of the plasma membrane
1. Introduction 2. A summary of membrane function 3. The structure of the plasma membrane 4. The dynamic nature of the plasma membrane
on the free-ribosomes in the cytosol 1) The transport of proteins into the nucleus 2) Importing mitochondria proteins 3) Import and sorting of Chloroplast (Chl)
1) The endoplasmic reticulum (ER) 2) Golgi complex (apparatus) 3) Lysosomes 4) Plant cell vacuoles 4. Synthesis, sorting and translocation of proteins synthesized in biosynthesis pathway and secretory pathway 1) Two types of proteins: synthesized on ER membane bound-
§6.The movement of substances across cell membrane 1. The energetics of solute movement and diffusion of substances through membrane 2. Four basic mechanism by which solute molecules move across membrane 1) Passive transport (1) simple diffusion (2) The diffusion of ions through membrane by hydrophilic channels (3) Facilitated diffusion 2) Active transport 3. Roles of movement of small molecules across membrane 4. Cellular homeostasis