2017北京分析测试学术报告暨展览会部分会议摘要

ANALYSIS OF GRAIN COMPOSITION IN MAINSTREAM SMOKE BY GC×GC-qMS

Xi ZHANG, Yueqi LI, Jun FAN, Taohong HUANG

Analytical Instruments Dept. Analytical Application Center Shimadzu (China) Co., LTD. 6F, Life Tower, No.16 Chaoyang Men Wai Street Chaoyang District 100020, Beijing, China

The chemical composition of flue gas is extremely complex. Some data show that there are about 3996 chemical components in flue gas. According to the different combustion mode flue gas is divided into mainstream and sidestream smoke. Mainstream smoke is constituted by gas, vapor state and other solid substance. The substance that can pass through Cambridge filter in room temperature are called gas phase material, and those retained called particle matter. The study of the composition of particulate phase is helpful to analyze the distribution of volatile and semi volatile substances in the flue gas. Also it is helpful to guide the production process to reduce the release of cigarette tar, at the same time selectively adding spices to make up for the dilution of flue gas. However, when using GCMS only, the separation degree and peak capacity are limited, and the matrix interference is serious in qualitative analysis.

Two-dimensional gas chromatography is a new method developed in 1990s to separate complex mixtures. A two dimensional gas chromatographic column system was connected together in series by a modulator. Two-dimensional gas chromatography has the advantages of higher resolution, large peak capacity, better sensitivity and faster analysis than ordinary one-dimensional gas chromatography. At present, two-dimensional gas chromatography has been applied in many fields, such as food, petrochemical products, essence, environmental research and so on.

In this study, two-dimensional gas chromatography-mass spectrometry was applied to the analysis of particulate components in mainstream smoke. The results show that two-dimensional gas chromatography-mass spectrometry provides a better means for the analysis of components of the mainstream smoke particles, which is of great significance for understanding the composition of the components in flue gas and guiding the production of cigarettes.

A MULTIDIMENSIONAL MATCHING APPROACH FROM CHEMICAL GROUP TO METABOLIC GROUP COMBINED WITH VITRO-TO-VIVO LONGITUDINAL ANALYSIS USING LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY

Guifang FENG1, 2, Shu LIU1,, Fengrui SONG1, Zhiqiang LIU1, *

1 National Center of Mass Spectrometry in Changchun & Jilin Province Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry & Chemical Biology Laboratory, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China;

2 University of Science and Technology of China, Hefei 230026, China. Ginseng has been considered one of the most valuable medicinal herbs for thousands of years, and now it is widely used for all various pharmacological activities [1]. To make clear the metabolic mechanism of ginseng is the guarantee of its therapeutic effects. The traditional analytical technologies on drug metabolism focus the attention on analyzing the constituents before and after administration. The comprehensive action in vivo mainly including intestinal bacterial metabolism [2] and liver cytochrome (CYP450) [3] can‘t be expatiated in specifically. The model in vitro considering the massive endogenous interference and describing the special drug metabolism pathway is necessary. We developed a multidimensional matching approach from chemical group to metabolic group combined with vitro-to-vivo longitudinal analysis. The application of ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) method was to obtain the mass information. There were metabolites of ginseng detected via intestinal microflora action in vitro, which biotransformed mainly through deglycosylation. The deglycation products of ginsenosides were then metabolized by redox reaction under the action of CYP450 in vitro. The corn step was to detect the constituent of ginseng after oral administration including the metabolites biotransformed by intestinal bacterial and CYP450 in vitro and other metabolites only produced in vivo. All the factors including matrix interference in vivo, multiple metabolizable action in vivo and trace amount detection were taken into consideration in this strategy. We anticipate that such an analytical approach will be extended to screen and characterize other traditional Chinese medicine metabolites in vitro and in vivo rapidly.