清华大学生物工程试题,历年完整版
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生物工程前沿(吴庆余授课部分)
考题与参考文献
思考题:
1.利用蓝细菌进行分子生物学研究和生物技术(生物工程)研究有哪些优点?
2.根据课堂讲授内容,参考有关文献,利用已经构建的frxC-蓝细菌突变体,提
出一个新的有意义的科学问题,并拟定一个解决该问题的实验技术路线。3.根据课堂讲授内容,参考有关文献,提出一个蓝细菌生物技术产品的开发思
路。
4.利用蓝细菌和重组DNA技术构建一个转基因突变体一般包括哪些实验步
骤?
5 举例说明,微藻细胞工程面临的问题和挑战,如何应对和解决。
1.Wu Qingyu and Wim Vermaas, 1995, Light-Dependent Chlorophyll a
Biosynthesis upon ChlN Deletion in Wild-Type and Photosystem I-less Strains of the Cyanobacterium Synechocystis sp PCC 6803. Plant Molecular Biology,V ol. 29∶933-945.
2.张大兵、吴庆余,1996,小球藻细胞的异养转化。植物生理学通讯,32卷,
第2期,140-144页。
3.徐红,吴庆余,1996,蓝藻光系统II的组成、相关基因和遗传修饰。南京
大学学报, 1996年第3期。485-493。
4.吴庆余,Wim Vermaas, 1996, 蓝细菌Synechocystis sp PCC 6803中ORF469
的分子克隆和缺失突变工程株的构建。生物工程学报,1996,第3期。311-317。
5.吴庆余,徐红,1997,蓝细菌Synechocystis sp PCC 6803中叶绿素的合成调
控类囊体膜重建的研究。植物学报,39(11): 1003-1009。
6.余九九、李宽钰、吴庆余,1997,螺旋藻的藻胆蛋白提取及稳定性研究,
海洋通报,16(4):24-26
7.Wu Qingyu, Junbiao Dai, Yoshihiro Shiraiwa, Guoying Sheng and Jiamo Fu,
1999, A renewable energy source – hydrocarbon gases resulting from pyrolysis of the marine nanoplanktonic alga Emiliania huxleyi. Journal of Applied Phycology, 11(2): 147-152.
8.Qingyu Wu, Yoshihiro Shiraiwa, Guoying Sheng and Jiamo Fu, 1999,
Hydrocarbons resulting from pyrolysis of the marine coccolithophores Emiliania huxleyi and Gephyrocapsa oceanica. Marine Biotechnology, 1(4):346-352. 9.戴俊彪、吴庆余,1999,培养海洋微藻Isocrysis galbana生产EPA和DHA。
海洋科学,1999(3):36-39。
10.Wu Qingyu, Yu Jiujiu and Zhao Nanming, 1999, Effects of chlorophyll
availability on fluorescence components of photosystems in the ORF469- deletion mutant of cyanobacterium. Tsinghua Science and Technology, 4(3): 1544-1550. 11.Yu Jiujiu, Wu Qingyu, Mao Haibing, Zhao Nanming and Wim Vermaas, 1999,
Effects of Chlorophyll Availability on the phycobilisomes in Synechocystis sp.
PCC 6803. IUBMB Life, 48(6): 625-630.
12.戴俊彪、吴庆余等,2000,利用基因工程方法研究生物标志化合物分子来源。
科学通报,45(1):69-75。
13.Dai Junbiao, Wu Qingyu et al., 2000, Investigation on molecular source of
biomarkers by genetic techniues. Chinese Science Bulletin. 45(11): 1025-1030. 14.Weimin Peng, Qingyu Wu & Pingguan Tu, 2000, Effects of temperature and
holding time on production of renewable fuels from pyrolysis of Chlorella protothecoides. Journal of Applied Phycology, 12: 147-152.
15.戴俊彪、吴庆余,2000,室内外培养单细胞微藻的生长及生化组成。海洋科
学,24:29-32。
16.彭卫民、吴庆余等,2000,采用热解技术将湖泊浮游藻类用于燃料生产。环
境污染治理技术与设备,1(3):24-28。
17.G. F. Wu, Q. Y. Wu and Zh. Y. Shen, 2000, Accumulation of poly-β
-hydroxybutyrate in cyanobacterium Synechocystis sp. PCC 6803. Bioresource Technology, 76: 85-90.
18.Weimin Peng, Qingyu Wu and Pingguan Tu, 2001, Pyrolytic characteristics of
heterotrophic Chlorella protothecoides for renewable bio-fuel production. Journal of Applied Phycology. 13: 5-12.
19.Wu Guifang, Shen Zhongyao, Wu Qingyu and Zhao Nan-ming, 2001 Molecular
cloning and construction of agp gene deletion-mutant in Cyanobacterium Synechocystis sp. PCC 6803. Acta Botanica Sinica, 43(5): 512-516.
20.Qingyu Wu, Jiujiu Yu and Nanming Zhao, 2001, Partial recovery of
light-independent chlorophyll biosynthesis in the chlL-deletion mutant of Synechocystis sp. PCC 6803. IUBMB Life, 51(5): 289-293.
21.Weimin Peng, Qingyu Wu, Pingguan Tu and Nanming Zhao, 2001, Pyrolytic
characteristics of microalgae as renewable energy source determined by thermogravimetric analysis. Bioresource Technology, 77: 1-7. (SCI)
22.吴桂芳、吴庆余、沈忠耀,2001,培养条件对蓝细菌生长及PHB积累的影
响,清华大学学报(自然科学版),41(6):30-33。
23.Guifang Wu, Zhongyaoshen and Qingyu Wu, 2001, Possible to improve the
cyanbacterial poly- -hydroxybutyrate biosynthesis level. Journal of Chemical Engineering of Japan, 34(9): 1187-1190.
24.Wu G F, Shen Z Y, Wu Q Y. Modification of Carbon Partitioning to Enhance
PHB Production in Synechocystis sp. PCC6803. Enzyme Microbi. Technol., 2002, 30(6): 710-715
25.Miao Xiaoling and Wu Qingyu, 2002, Inorganic carbon utilization in some