中国猪繁殖与呼吸综合征的现状--杨汉春

NVSL 97-7985HIBA-21MN(-sM4NChG-M)1N1M2/6281NO28408C14A0POR48H521CRF4O2-OSOBF5LO19RX5RMRaORFOI1FSFOYF5R1RU5505VRFO-F1P5RF1G5R265OZF32H1R43541N2PFB01LO519OORC7RR-CF1F-F51O55ORRFF55 strain SP ORF5
Hale Waihona Puke Baidu
ALemlyersvtaadcMvPiMRrNMuRN1sNS81N148N8C4s4psABpN22NsNGpssDM2pp2Y22 NNsspp22
NVSL 97-7P9Nr8simC5tra1eIiA6LnPI1P8MSaS6141cU2P-5Y244N-9NNP-4CNsG2VBNRsCspNHsZpRepN2p-sNsNs21122pp2ps11s3P2Np2PpB3R0N2Ls221RJp-9sSN4p27M2-sN1pLs2N0pV12sNpNJP2Ls1p1.221N01sp2 CH-1a Nsp2
HB-2(sh)/2002 Nsp2
HuN1001 BJ1006
HB-1(sh)B/SH2JH00XE70BBNNH02JB-61N1GN1-B0NCs4DH0s0pJGsps2N22upyN2NXNN20ssXLp6spN220p020N6s720pNsN82GpMsNS2Bp2sN2J0p0Ns2p8s2pN2sp2
0.05
ORF5
SXL2N0S01YJ90X00O6B11R00HJF801S5E10ODN0BRB111BFJJ10151JF000HH101S3000BE5G07O1NZ8SZR11JZD01F11J101510100011101023 HuN1002
LN1101 DC ORF5
geneBJ0191HH0B1EGH1N0XB10111HOJ00HLHR00uH12E2FuB1H5HLNN10Ju1L0211C1N0H1J10GH1010G10E32911ZE1N0HO01N211U0R100BF00207521ORF5
我国PRRSV毒株的多样性 Diversity of strains
Nsp2基因缺失/插入毒株的存在 Nsp2 gene deletion/insertion strain
21-aa
22-aa 36-aa 25-aa 30-aa 18-aa
Nsp2编码区存在36-aa插入的毒株 36-aa insertion in Nsp2 coding region
HLJ1101 HLJ1102 SDH1uB1JN0G111BZ0T0J11JS0100130D0ZH1H0J11Hu0G5Gu101BN1X0NX11041111120100000026112
LAemlyesrtvaadcvPirRRueRssSpOPORRFRR5FBS5J0M-14LNOVPR1JO.FDL2R51YOF1RO051FR5F5
问题1
猪繁殖与呼吸综合征病毒毒株的多样性 Diversity of porcine reproductive and respiratory virus (PRRSV) strains
我国流行的PRRSV以北美洲型（基因2型）毒
株为主，但呈现致病性和基因组变异的多样性 In China, majority of PRRSV belongs to North American strain (genotype 2), with diversity of pathogenicity and genomic variation.
临床疫情一年四季均可发生
The clinical outbreaks caused by HP-PRRSV occur in all seasons. 饲养管理水平高的规模猪场相对较平稳
PRRS exhibits stable situation in intensive pig farms with good management 疫情在中、小型猪场与散养户多发
The outbreaks caused by HP-PRRSV occur in middle-, small-size pig farms and backyard farms.
HP-PRRSV感染的临床症状呈现多样化 The affected pig herds exhibit diversity of clinical sign.
2013-10-15
内容提要 Outline
猪繁殖与呼吸综合征的流行现状 Current situation of PRRS in China
猪繁殖与呼吸综合征防控中的问题 Problems of PRRS prevention and control in China
繁殖与呼吸综合征的控制对策 Control strategy of PRRS
高致病性猪繁殖与呼吸综合征病毒（HP-PRRSV） 的出现和广泛流行，加剧了该病的复杂性和控制 难度。 The emergence and extensive prevalence of HPPRRSV in 2006 further aggravate the complexity of PRRS and its difficulty of controlling in China.
为什么猪繁殖与呼吸综合难以控制？
极易变异 mutation
极易重组 recombination
PRRSV
毒株的多样性 diverse strains 持续感染 persistence
病毒增殖的抗体依赖性作用 increased Ab dependency
神秘的致病和免疫机制 mystery cause and immune mechanism
体温升高 high fever, over 41 ℃ 呼吸道症状（喘气、咳嗽、呼吸困难） Respiratory conditions
消化道症状（腹泻）diarrhea
神经症状 nerve signs
母猪繁殖障碍（流产） reproductive failures in pregnant sows
种猪群的亚临床感染和带毒 subclinical infection and virus-carrier in breeding herds
我国的基因2型PRRSV至少可以分为三个亚群 ，其中大多数属于亚群3 PRRSV in China can be divided into three subgroups at least, and majority of PRRSV is clustered into subgroup 3, with multiple branches.
2006-2007年由高致病性猪繁殖与呼吸综合征病 毒所致PRRS疫情的大爆发之后，逐渐变得缓和 ，目前仍有散发性疫情发生，呈现地方流行性。 PRRS caused by HP-PRRSV is sporadic and endemic after epidemic of 2006-2007.
高致病性猪繁殖与呼吸综合征病毒的毒力仍未降 低，发病猪群仍呈高发病率和高死亡率。 HP-PRRSV remains high virulence for pigs. The affected pig herds show high morbidity and mortality.
0J0W7S7UyHJHixHEJaE1nBXNNg1TsNxJ4pisHN-32psU3NHp2sNBU2spN1Bsp22p2N2sNps2p2
BSJSC1GhS11Da0H1Ha10QJYnuHT1XYxYJNTNJiXwN-1N4P2N2AsnN09NpNNs001sp2sN6s8spp2pNp2sN22N2psss2ppp222 07BJ Nsp2 GD Nsp2 07QN Nsp2 J0LGLH8NZ1HE11NNu00N2s10p12N203sp2
YSJhDHXaXTaOBA0nBJH1HR71JxBHW1JOHSFi-UO-JJs0G5U2yR1yiE5URBaDx1O0BFNnFH206R5Og1O52O1OFOxRRO5RiRORF-FR3FF3RF5F55O5F55RF5 HEB1 ORF5
080H7JXBHuNJ1uLH4GONNO0u3R4DSR7YNNTOOFHHFOBQNXP5RRO5EHRJY9F0FROBFO5165OOF5RTRO5ORRJFFRRFFO55F55FR55F5
许多猪场多个毒株共存，可以分离或检 测到两个以上的毒株 Multiple strains of PRRSV co-exist in swine farms. More than two strains of PRRSV can be isolated or detected from one pig farm.
发过病的猪场——母猪带毒，可以排毒 Sows can be virus-carrier and can shed virus after outbreak.
猪场的主要传染来源 HP-PRRSV shed by sows is the major origin of virus for pig farms.
Prime Pac ORF5 HB-1(sh)/20H0S2EDBSNNBOHHJ1B0J1R0BHB1-7104F10L00OH35610JO21uR06OR1N3F0RF1535FZ050J13102
2010-2011
G JDX0Ow7RnFN056XMLGOO2SR0RY2F0F05N85028O00RO8FR5FO5RF5
0.2
Nsp2
HEHNE1BN1J10H110F0B00S02194HN1HH0BsBE4p1BSJN20D1L1D1B0H00NC11BN0J0u01132sJNB000p11s20010p11002071 BJ1008 LN1101
ZJ1001 JX1002
geneHBY1D1H0N5EHBspL12JNZ1ZJs1p1JH02113SHu10HYN03Be1S011nS60X1HHaD0H00n02BB8213-J9E010X11NH0N011Ns913U001p0sN1220pB002s17p32Nsp2
哺乳仔猪、保育猪或生长育肥猪感染和发病 Suckling piglets, nursery and growing pigs show clinical signs once affected.
二、猪繁殖与呼吸综合征防控中的问题
Problems of PRRS prevention and control in China
Leman China Swine Conference
中国猪繁殖与呼吸综合征的现状、 问题与对策
Current situation, problems and control strategy of PRRS in China 杨汉春, Hanchun Yang
中国农业大学动物医学院
College of Veterinary Medicine, China Agricultural University
高致病性毒株 HP-PRRSV 低致病性毒株 LP-PRRSV 疫苗毒株 Vaccine PRRSV 重组毒株 Recombinant PRRSV 疫苗演化而来的毒株 PRRSV evolved from vaccine virus
2006-2008
Nsp2 gene
ORF5 gene
2009
HB1104 STDJ1BJ1J011B0X0JB011ZJ1100JHB1030Ge1JH0S002n010X47aBD0021n1111Q0-10001N00124OORRFF55
一、猪繁殖与呼吸综合征的流行现状
Current situation of PRRS in China
猪繁殖与呼吸综合征已成为危害中国养猪生产的 第一大病 PRRS is the first ranking disease that impacts swine production in China.