Splicing_基因剪辑
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© 2014 Pearson Education, Inc.
3. What role do proteins play in the splicing of group I self-splicing introns in vivo? A. Proteins shield the negative charges on the RNA backbone. B. Proteins recruit spliceosome components to splice sites by binding to exonic-splicing enhancers. C. Together with snRNAs, proteins form the spliceosome complex. D. Proteins cleave the 5 and 3 splice sites.
RNA splicing
课后作业
2016-10-27
© 2014 Pearson Education, Inc.
1. The most highly conserved splice and branch site sequences recognized within the introns of human nuclear pre-mRNA by major and minor spliceosomes include which of the following?
A. exons 1, 2, 4 joined together or exons 1, 3, 4 joined together B. exons 1, 2, 4 joined together C. exons 1, 3, 4 joined together D. exons 1, 2, 3 joined together or exons 1, 2, 3, 4 joined together
A. 5 splice site GU or AU, 3 splice site AG or AC, branch site A
B. 5 splice site GU or AU, 3 splice site AG or AC, branch site A or G C. 5 splice site GU, 3 splice site AG, branch site A
© 2014 Pearson Education, Inc.
5. RNA splicing activities can be regulated in cells by various activator and repressor proteins. Which of the following proteins is a pair of different splicing activators?
© 2014 Pearson Education, Inc.
4. Consider a pre-mRNA that consists of four exons and three introns in the following arrangement:
5-exon 1/intron 1/exon 2/intron 2/exon 3/intron 3/exon 4-3
C. cytidine deaminase
D. RNA lFra Baidu bibliotekgase
© 2014 Pearson Education, Inc.
Question 8. Are the 5′ splice site and 3′ splice site labeled 5′ and 3′ with respect to the ends of the intron or the ends of the exons? In addition to the 5′ and 3′ splice sites, what other sequence is required in splicing? Where is this sequence located? Question 9. Starting with an unspliced, pre-RNA, describe the intermediates produced after the first step in the splicing reaction. Question 10. Given that the two key reactions for splicing could proceed in the forward or reverse direction, what prevents the splicing reactions from proceeding backward in vivo
D. 5 splice site AU, 3 splice site AC, branch site A
© 2014 Pearson Education, Inc.
2. For group I self-splicing introns, which of the following occurs as a consequence of the second transesterification reaction of the splicing pathway—i.e., nucleophilic attack by the 3 OH of the 5 exon on the 3 splice site?
List all alternative mature mRNA splice forms that could arise if this pre-mRNA underwent spliceosome-mediated splicing. Only consider mature mRNA(s) that do not retain intron(s).
A. exon shuffling B. alternative splicing C. mutually exclusive splicing D. site-specific deamination
© 2014 Pearson Education, Inc.
7. Trypanosomes have a novel post-transcriptional process for editing mitochondrial RNA. In this form of RNA editing, several uracil bases are inserted at specific locations in an mRNA (e.g., for purposes of shifting the reading frame into correct position). The insertions are directed by short guide RNAs (gRNAs) that encode editing “instructions” in the form of complementary base-pairing sequences. Which enzyme involved in this process catalyzes the addition of uracil (U) bases to the mRNA? A. TUTase B. endonuclease
A. The two exons flanking the intron are joined together and a linear-shaped intron is released.
B. The two exons flanking the intron are joined together and a lariat-shaped intron is released.
A. Half-pint protein and SF2/ASF protein B. hnRNP protein and SF2/ASF protein C. Half-pint protein and hnRNP protein D. SC35 protein and hnRNP protein
The splice sites within each intron are the following (with the 5 splice sequence followed by the 3 splice sequence):
intron 1 = GU and AG intron 2 = AU and AG intron 3 = AU and AC
© 2014 Pearson Education, Inc.
© 2014 Pearson Education, Inc.
6. The process of constructing new genes via the loss or gain of domains during the evolution of a protein family can be best explained by which phenomenon?
© 2014 Pearson Education, Inc.
Question 11. Describe the product of a splicing reaction in which the spliceosome recognizes a “pseudo” 3′ splice site within the intron instead of the actual 3′ splice site. The “pseudo” site is slightly upstream of the actual 3′ splice site. How could this alter the protein product after translation? Question 12. Explain how steric hindrance can lead to mutually exclusive splicing. Question 13. How does nonsense-mediated decay contribute to determining the final alternatively spliced products available for translation?
C. The two exons flanking the intron are joined together and the 5 end of the intron is joined to a conserved branch site A nucleotide. D. The two exons flanking the intron are joined together in a spliceosome-dependent reaction.
3. What role do proteins play in the splicing of group I self-splicing introns in vivo? A. Proteins shield the negative charges on the RNA backbone. B. Proteins recruit spliceosome components to splice sites by binding to exonic-splicing enhancers. C. Together with snRNAs, proteins form the spliceosome complex. D. Proteins cleave the 5 and 3 splice sites.
RNA splicing
课后作业
2016-10-27
© 2014 Pearson Education, Inc.
1. The most highly conserved splice and branch site sequences recognized within the introns of human nuclear pre-mRNA by major and minor spliceosomes include which of the following?
A. exons 1, 2, 4 joined together or exons 1, 3, 4 joined together B. exons 1, 2, 4 joined together C. exons 1, 3, 4 joined together D. exons 1, 2, 3 joined together or exons 1, 2, 3, 4 joined together
A. 5 splice site GU or AU, 3 splice site AG or AC, branch site A
B. 5 splice site GU or AU, 3 splice site AG or AC, branch site A or G C. 5 splice site GU, 3 splice site AG, branch site A
© 2014 Pearson Education, Inc.
5. RNA splicing activities can be regulated in cells by various activator and repressor proteins. Which of the following proteins is a pair of different splicing activators?
© 2014 Pearson Education, Inc.
4. Consider a pre-mRNA that consists of four exons and three introns in the following arrangement:
5-exon 1/intron 1/exon 2/intron 2/exon 3/intron 3/exon 4-3
C. cytidine deaminase
D. RNA lFra Baidu bibliotekgase
© 2014 Pearson Education, Inc.
Question 8. Are the 5′ splice site and 3′ splice site labeled 5′ and 3′ with respect to the ends of the intron or the ends of the exons? In addition to the 5′ and 3′ splice sites, what other sequence is required in splicing? Where is this sequence located? Question 9. Starting with an unspliced, pre-RNA, describe the intermediates produced after the first step in the splicing reaction. Question 10. Given that the two key reactions for splicing could proceed in the forward or reverse direction, what prevents the splicing reactions from proceeding backward in vivo
D. 5 splice site AU, 3 splice site AC, branch site A
© 2014 Pearson Education, Inc.
2. For group I self-splicing introns, which of the following occurs as a consequence of the second transesterification reaction of the splicing pathway—i.e., nucleophilic attack by the 3 OH of the 5 exon on the 3 splice site?
List all alternative mature mRNA splice forms that could arise if this pre-mRNA underwent spliceosome-mediated splicing. Only consider mature mRNA(s) that do not retain intron(s).
A. exon shuffling B. alternative splicing C. mutually exclusive splicing D. site-specific deamination
© 2014 Pearson Education, Inc.
7. Trypanosomes have a novel post-transcriptional process for editing mitochondrial RNA. In this form of RNA editing, several uracil bases are inserted at specific locations in an mRNA (e.g., for purposes of shifting the reading frame into correct position). The insertions are directed by short guide RNAs (gRNAs) that encode editing “instructions” in the form of complementary base-pairing sequences. Which enzyme involved in this process catalyzes the addition of uracil (U) bases to the mRNA? A. TUTase B. endonuclease
A. The two exons flanking the intron are joined together and a linear-shaped intron is released.
B. The two exons flanking the intron are joined together and a lariat-shaped intron is released.
A. Half-pint protein and SF2/ASF protein B. hnRNP protein and SF2/ASF protein C. Half-pint protein and hnRNP protein D. SC35 protein and hnRNP protein
The splice sites within each intron are the following (with the 5 splice sequence followed by the 3 splice sequence):
intron 1 = GU and AG intron 2 = AU and AG intron 3 = AU and AC
© 2014 Pearson Education, Inc.
© 2014 Pearson Education, Inc.
6. The process of constructing new genes via the loss or gain of domains during the evolution of a protein family can be best explained by which phenomenon?
© 2014 Pearson Education, Inc.
Question 11. Describe the product of a splicing reaction in which the spliceosome recognizes a “pseudo” 3′ splice site within the intron instead of the actual 3′ splice site. The “pseudo” site is slightly upstream of the actual 3′ splice site. How could this alter the protein product after translation? Question 12. Explain how steric hindrance can lead to mutually exclusive splicing. Question 13. How does nonsense-mediated decay contribute to determining the final alternatively spliced products available for translation?
C. The two exons flanking the intron are joined together and the 5 end of the intron is joined to a conserved branch site A nucleotide. D. The two exons flanking the intron are joined together in a spliceosome-dependent reaction.