灰霉菌基因Bgs3致病功能研究[毕业作品]
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第一章 实验相关材料与方法·········································································· 6 第一节 实验相关材料 ················································································ 6 1.1 供试菌株 ·························································································· 6 1.2 供试载体 ·························································································· 6 1.3 相关引物 ·························································································· 6 第二节 主要药品试剂与仪器··································································· 6 2.1 主要药品试剂 ················································································· 6 2.2 仪器 ··································································································· 7 第三节 培养基的配制 ················································································ 7 3.1 LB 培养基 ························································································· 7 3.2 PDB 培养基····················································································· 7 3.3 MM 培养基： ················································································ 8 3.4 IM 培养基： ··················································································· 8 第四节 实验方法 ························································································· 9 4.1 配置反应体系 ················································································· 9 4.2 DNA 凝胶回收 ·················································································· 9 4.3 质粒的酶切····················································································10 4.4 乙醇沉淀法片段回收··································································10 4.5 质粒连接 ························································································10 4.6 大肠杆菌 DH5α 感受态转化 ····················································11 4.7 电击转化农杆菌···········································································11 4.8 电泳检测 ························································································11
中文题目 英文题目
灰霉菌基因 Bgs3 致病功能研究 A study about the Pathogenicity of Botrytiscinerea Bgs3
中文摘要
灰霉菌 (Botrytis cinerea) 一种死体营养型真菌，其又被广泛称为灰葡萄 孢能感染超过 1400 种植物，其中包括几乎所有的蔬菜和果树。灰霉病感染不仅 在植物生长期病害，水果和蔬菜的储藏期也可以发病，因此灰霉菌每年会造成数 百亿美元的经济损失。由于灰霉菌的寄主范围广泛，所以其生产上也危害严重， 而现在相关分子的研究技术日趋成熟，因而灰霉病菌也受到广泛研究，但关于其 分子致病机理目前尚无定论。β-葡萄糖苷酶作为在真菌中广泛存在的酶类，β葡萄糖苷酶对真菌的生长发育也有很大影响。为了研究 Bgs 基因的缺失会对灰霉 菌β-葡萄糖苷酶的合成的影响，并进而对灰霉菌致病力的影响，为了阐释 Bgs3 基因在灰霉病菌生长发育和致病过程的调控作用，构建灰霉菌 Bgs3 转录因子的 敲除载体，以期得到 Bgs3 转录因子的缺失突变体。
Key words: Botrytis cinerea; transcription factor;Bgs3; knockout vector
II
目录Βιβλιοθήκη Baidu
前言························································································································· 1 第一节 灰葡萄孢菌与灰霉病··································································· 1 第二节 葡萄糖苷酶的作用与研究进展 ················································· 3 第三节 本研究的目的和意义··································································· 4
关键词：灰霉菌；转录因子；Bgs3；敲除载体
I
Abstract
Botrytis cinerea is a deadly nutritive fungus, which is widely known as Botrytis cinerea, infects more than 1,400 species, including almost all vegetables and fruit trees. Botrytis cinerea infection can not only occur during plant growing periods, during the storage of fruits and vegetables, so that gray molds can cause tens of billions of dollars in economic losses each year. Due to the wide range of hosts of Botrytis cinerea, its production is also harmful, and now the research technology of related molecules is becoming more and more mature, so the pathogen of Botrytis cinerea is also widely studied, but its molecular pathogenesis is still inconclusive. Β-glucosidase as a fungus in the widespread presence of enzymes, β-glucosidase on the growth and development of fungi also have a great impact. In order to study the effect of Bgs gene deletion on the mycelia of Botrytis cinerea and to the pathogenicity of Botrytis cinerea in order to study the effect of Bgs3 gene on the growth and pathogenesis of Botrytis cinerea, The knockout vector of the gray matter Bgs3 transcription factor, in order to obtain a deletion mutant of the Bgs3 transcription factor.
中文题目 英文题目
灰霉菌基因 Bgs3 致病功能研究 A study about the Pathogenicity of Botrytiscinerea Bgs3
中文摘要
灰霉菌 (Botrytis cinerea) 一种死体营养型真菌，其又被广泛称为灰葡萄 孢能感染超过 1400 种植物，其中包括几乎所有的蔬菜和果树。灰霉病感染不仅 在植物生长期病害，水果和蔬菜的储藏期也可以发病，因此灰霉菌每年会造成数 百亿美元的经济损失。由于灰霉菌的寄主范围广泛，所以其生产上也危害严重， 而现在相关分子的研究技术日趋成熟，因而灰霉病菌也受到广泛研究，但关于其 分子致病机理目前尚无定论。β-葡萄糖苷酶作为在真菌中广泛存在的酶类，β葡萄糖苷酶对真菌的生长发育也有很大影响。为了研究 Bgs 基因的缺失会对灰霉 菌β-葡萄糖苷酶的合成的影响，并进而对灰霉菌致病力的影响，为了阐释 Bgs3 基因在灰霉病菌生长发育和致病过程的调控作用，构建灰霉菌 Bgs3 转录因子的 敲除载体，以期得到 Bgs3 转录因子的缺失突变体。
Key words: Botrytis cinerea; transcription factor;Bgs3; knockout vector
II
目录Βιβλιοθήκη Baidu
前言························································································································· 1 第一节 灰葡萄孢菌与灰霉病··································································· 1 第二节 葡萄糖苷酶的作用与研究进展 ················································· 3 第三节 本研究的目的和意义··································································· 4
关键词：灰霉菌；转录因子；Bgs3；敲除载体
I
Abstract
Botrytis cinerea is a deadly nutritive fungus, which is widely known as Botrytis cinerea, infects more than 1,400 species, including almost all vegetables and fruit trees. Botrytis cinerea infection can not only occur during plant growing periods, during the storage of fruits and vegetables, so that gray molds can cause tens of billions of dollars in economic losses each year. Due to the wide range of hosts of Botrytis cinerea, its production is also harmful, and now the research technology of related molecules is becoming more and more mature, so the pathogen of Botrytis cinerea is also widely studied, but its molecular pathogenesis is still inconclusive. Β-glucosidase as a fungus in the widespread presence of enzymes, β-glucosidase on the growth and development of fungi also have a great impact. In order to study the effect of Bgs gene deletion on the mycelia of Botrytis cinerea and to the pathogenicity of Botrytis cinerea in order to study the effect of Bgs3 gene on the growth and pathogenesis of Botrytis cinerea, The knockout vector of the gray matter Bgs3 transcription factor, in order to obtain a deletion mutant of the Bgs3 transcription factor.