Hoagland 营养液配方(试验总结)

Hoagland 营养液配方，

荷格伦特营养液的配方如下:

2.微量元素每升培养液中加入的毫克数

3.每升培养液中加入2 mL FeEDTA溶液(即乙二胺四乙酸铁盐溶液)。

铁盐溶液：七水硫酸亚铁2.78g 乙二胺四乙酸二钠（EDTA-Na2）3.73g 蒸馏水500 mL

配制500 mL的铁盐溶液：

1.取两只洁净干燥烧杯，分别加入3.73 g EDTA-Na2和

2.78 g FeSO4·7H2O固体。

2.取1 L纯水煮沸，冷却至70℃后，量取300 mL，加入装有EDTA-Na2的烧杯中，进行搅

拌溶解。

3.量取200 mL纯水，冷却后加入另一烧杯中，搅拌溶解。

4.然后将FeSO4·7H2O溶液缓缓加入到EDTA-Na2溶液中，边加边搅拌。

5.最后，将铁盐溶液倒入棕色瓶中（黑暗），低温保存。

通常应当先配出各种盐类的浓缩液。注意避免浓缩液中出现沉淀。使用时按一定的比例加水稀释到要求的浓度。采用循环供液时，营养液中的矿质元素被植物体吸收后，应当及时进行调节，使营养液仍旧符合原配方的要求。

以下是老外的有些看不懂呵呵

Recipe for Hoagland's Complete Nutrient Solution

This is made essentially according to the following reference: D.R. Hoagland and D.I. Arnon. The water-culture method of growing plants without soil. Calif. Agr. Expt. Sta. Circ. 347. 1950. There is one change and that is in the form of iron added.

Prepare the following stock solutions (1-6) and use the amounts indicated to prepare 1 liter ( final volume ) of nutrient solution:

1. 1.00 M NH4H2PO4 use 1 mL/L of nutrient solution

2. 1.00 M KNO3 use 6 mL/L of nutrient solution

3. 1.00 M Ca(NO3 )2 use 4 mL/L of nutrient solution

4. 1.00 M MgSO4 use 2 mL/L of nutrient solution

Micronutrient stocks: combine the following amount of salts in a total volume of one liter of water, and then use 1 mL/L of this entire stock mixture (5) along with the stocks above (1-4) and the iron

stock (6) described below to make up a total of 1 L of nutrient solution.

5. 2.86 gm H3BO3

1.81 gm MnCl2 .4H2O

0.22 gm ZnSO4 .7H2 O

0.08 gm CuSO4 . 5H2O

0.02 gm H2MoO4 . H2O

(Assaying 85% MoO3)

6. Iron stock: to the above 5 stocks add 0.25 ml of this iron stock for 1 liter of nutrient solution.

To make up the iron stock, take 26.1 g EDTA and dissolve in 286 ml water that has ~19 g KOH . Then dissolve 24.9 FeSO4.7H2O in ~ 500 ml water. Slowly add the iron sulfate solution to the potassium EDTA solution and aerate this solution overnight with stirring. The pH rises to about 7.1 and the solution is wine red and very little precipitation occurs. Make to 1 liter final volume and store in a bottle covered with foil (dark).

Note: Hoagland's recipe called for 1 ml of 0.5% iron tartrate stock per liter of nutrient solution but we use the above substitution.