IWP L6_1427782-89-5_DataSheet_MedChemExpress

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美国FDA批准一种用于阿尔茨海默病早期诊断的检测仪

美国FDA批准一种用于阿尔茨海默病早期诊断的检测仪

美国FDA批准一种用于阿尔茨海默病早期诊断的检测仪夏训明(编译)
【期刊名称】《广东药科大学学报》
【年(卷),期】2022(38)3
【摘要】美国FDA于2022年5月4日批准Fujirebio诊断公司(Fujirebio Diagnostics,Inc.)研发的一种名为“The Lumipulse G β-Amyloid Ratio(1-42/1-40)test”的体外诊断检测仪,用于检测与阿尔茨海默病(Alzheimer’s Disease)相关的淀粉样斑块(amyloid plaques),可有效改进阿尔茨海默病的早期诊断。

【总页数】1页(P48-48)
【作者】夏训明(编译)
【作者单位】不详
【正文语种】中文
【中图分类】R74
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IT-158BS TC 中等热转变温度(Tg)多功能填充环氧树脂和酚醛固化无铅层压板和预浸料说明书

IT-158BS TC 中等热转变温度(Tg)多功能填充环氧树脂和酚醛固化无铅层压板和预浸料说明书

IT-158BS/IT-158TCMultifunctional Filled Epoxy Resin and Phenolic-Cured Lead Free Laminate & PrepregIT-158 is a medium Tg (>150℃ by DSC) multifunctional filled epoxy with high thermal reliability and CAF resistance. It’s suitable for industrial PCB, automobile and can pass 260℃ Lead free assembly.Key Features =============================== Advanced Resin TechnologyIndustrial standard material with medium Tg (150℃ by DSC) multifunctional filled epoxy resin and excellent thermal reliability.Lead-Free Assembly CompatibleRoHS compliant and suitable for high thermal reliability needs, and Lead free assemblies with a maximum reflow temperature of 260℃. Friendly Processing and CAF ResistanceFriendly to PCB process that users can easily handle the process by current equipment and chemical.CAF ResistanceExcellent thermal reliability and CAF resistance providing long-term reliability for industrial boards and automobile application.Available in Variety of ConstructionsAvailable in a various of constructions, copper weights and glass styles, including standard(HTE), RTF and VLP copper foil. ApplicationsPC and Notebook Memory ModuleGame PlayerMultilayer PCB AutomobileServers and Networking Telecommunications Heavy Copper ApplicationIndustrial Approval UL 94 V-0IPC-4101C Spec / 99 RoHS CompliantREV 09-14ITEQ Laminate/ Prepreg : IT-158TC / IT-158BSIPC-4101C Spec /99LAMINATE (IT-158TC)Thickness<0.50 mm[0.0197 in] Thickness≧0.50 mm[0.0197 in] Units T est MethodPropertyTypical Value Spec Typical Value SpecMetric(English)IPC-TM-650(or as noted)Peel Strength, minimumA. Low profile copper foil and very low profile copperfoil - all copper weights > 17µm [0.669 mil]B. Standard profile copper foil1.After Thermal Stress2.At 125°C [257 F]3.After Process Solutions 0.88 (5.0)1.58 (9.0)1.31 (7.5)1.14 (6.5)0.70 (4.00)0.80 (4.57)0.70 (4.00)0.55 (3.14)0.88 (5.0)1.66 (9.5)1.40 (8.0)1.23 (7.0)0.70 (4.00)1.05 (6.00)0.70 (4.00)0.80 (4.57)N/mm(lb/inch)2.4.82.4.8.22.4.8.3Volume Resistivity, minimumA. C-96/35/90B. After moisture resistanceC. At elevated temperature E-24/125 3.0x1010--5.0x1010106--103--5.0x10101.0x1010--104103MΩ-cm 2.5.17.1Surface Resistivity, minimumA. C-96/35/90B. After moisture resistanceC. At elevated temperature E-24/125 1.0x1010--5.0x1010104--103--1.0x10103.0x1010--104103MΩ 2.5.17.1Moisture Absorption, maximum -- -- 0.08 0.5 % 2.6.2.1 Dielectric Breakdown, minimum -- -- 60 40 kV 2.5.6 Permittivity (Dk, 50% resin content)(Laminate & Laminated Prepreg)A. 1MHzB. 1GHzC. 2GHzD. 5GHzE. 10GHz 4.34.34.24.14.05.44.44.34.24.14.05.4 --2.5.5.92.5.5.13Loss Tangent (Df, 50% resin content) (Laminate & Laminated Prepreg)A. 1MHzB. 1GHzC. 2GHzD. 5GHzE. 10GHz 0.0160.0160.0170.0180.0190.0350.0160.0160.0170.0180.0180.035 --2.5.5.92.5.5.13Flexural Strength, minimumA. Length directionB. Cross direction ----------------450-480(65,250-69,600)370-400(53,650-62,350)415(60,190)345(50,140)N/mm2(lb/in2)2.4.4Arc Resistance, minimum 125 60 125 60 s 2.5.1 Thermal Stress 10 s at 288°C [550.4F],minimumA. UnetchedB. Etched PassPassPass VisualPass VisualPassPassPass VisualPass VisualRating 2.4.13.1Electric Strength, minimum(Laminate & Laminated Prepreg)45 30 -- -- kV/mm 2.5.6.2 Flammability,(Laminate & Laminated Prepreg)V-0 V-0 V-0 V-0 Rating UL94 Glass Transition Temperature(DSC) 155 150 minimum 155 150 minimum ˚C 2.4.25Decomposition Temperature-- -- 345 325 minimum ˚C2.4.24.6 (5% wt loss)X/Y Axis CTE (40℃ to 125℃) -- -- 11-13 -- ppm/˚C 2.4.24 Z-Axis CTEA. Alpha 1B. Alpha 2C. 50 to 260 Degrees C ------------402403.360 maximum300 maximum3.5 maximumppm/˚Cppm/˚C%2.4.24Thermal ResistanceA. T260B. T288 -------->60>3030 minimum5 minimumMinutesMinutes2.4.24.1CAF Resistance -- -- Pass AABUS Pass/Fail 2.6.25The above data and fabrication guide provide designers and PCB shop for their reference. We believe that these information are accurate, however, the data may vary depend on the test methods and specification used. The actual sales of the product should be according to specification in the agreement between ITEQ and its customer. ITEQ reserves the right to revise its data at any time without notice and maintain the best information available to users.REV 09-14。

艾森生物 实验室耗材和试剂 目录t

艾森生物 实验室耗材和试剂 目录t
G1626-100G T-200-Y
21127-030
P0018 31458
20W 500ml 500ml 100ml PL009 PL017 353002 3516
11644807001
2227S/100ul 3175S/100ul 7002s/100ul 7004s/100ul 9803s/15ml G7126-1KG
胎牛血清FBS MEM
FX 384 30ul 枪头
Horse serum马血清
DMSO
PMSF
25ml移液管
DMEM 细胞筛网 70um
细胞培养皿 65×15mm
384孔板
真空抽气泵
BSA牛血清蛋白
EGF Receptor (D38B1) XP® Rabbit mAb
western 一抗二抗去除液(弱碱性)
96孔细胞培养板 细胞网筛
25ml移液管 96孔板
无酚红1640 0.1-20ul排枪枪头 10-250ul排枪枪头 1ml盒装蓝色枪头 Trypsin EDTA,0.05%
DMSO 医用酒精棉球
医用酒精棉 麻面无粉手套 1ml灭菌盒装蓝枪头
DMEM L-15 384 HT assay plates
P3563-10PAK
PL017
SM0671/2x250ul
PL009 4685s/100ul 9271s/100ul
9101s/200ul
9102s/200ul 7002s/100ul 7004s/100ul 9803s/15ml
3777s/100ul
21127-022
11644807001 11966-025 6570 SD6031 华东 CU50
Gibco 华东 华东 Roche Gibco CORNING 上海生工 M号 中新 迪申 sigma AXYGEN Gibco

HT-2157_DataSheet_MedChemExpress

HT-2157_DataSheet_MedChemExpress

Inhibitors, Agonists, Screening Libraries Data SheetBIOLOGICAL ACTIVITY:HT–2157 (SNAP 37889) is a selective, high–affinity, competitive antagonists of galanin–3 receptor (Gal 3).IC50 & Target: Galanin–3 receptor [1]In Vitro: HT–2157 (SNAP 37889) binds with high affinity to membranes from transiently transfected LMTK – cells expressing the human Gal 3 receptor (K i =17.44±0.01 nM; n>100) and is highly selective for Gal 3 over the Gal 1 and Gal 2 subtypes (K i >10,000 nM for each subtype; n=46 of each subtype). When tested for the antagonism of galanin–evoked inhibition of adenylyl cyclase, HT–2157 (0.1–10μM) produces concentration–dependent rightward shifts of the concentration–effect curve to galanin [1].In Vivo: The galanin–3 receptor antagonist, HT–2157 (SNAP 37889), reduces operant responding for ethanol in alcohol–preferring rats. The novel selective GALR3 antagonist, HT–2157, to reduce anxiety–like behaviour and voluntary ethanol consumption in the iP (alcohol–preferring) rat. Male iP rats treated with HT–2157 at a dose of 30 mg/kg (i.p.) do not show altered locomotor activity or changes in anxiety–like behaviour in the elevated plus maze or light–dark paradigms. Treatment with HT–2157 (30 mg/kg, i.p.)reduces operant responding for solutions containing ethanol, sucrose and saccharin. Collectively, results from the current study shows that HT–2157 (30 mg/kg, i.p.) is effective in reducing operant responding for ethanol, independent of a sedative effect [2].PROTOCOL (Extracted from published papers and Only for reference)Kinase Assay:[1]Binding affinities for HT–2157 (SNAP 37889) and SNAP 398299 at the human Gal 1, Gal 2, and Gal 3 receptors are determined by using the 125I–galanin displacement assay. Additionally, is tested for binding in a broad cross–reactivity panel that included G–protein–coupled receptors, ion channels, enzymes, and transporters. The ability of HT–2157 to antagonize functional responses to galanin is examined in modified HEK–293 cells (PEAK rapid cells) transiently cotransfected with the Gal 3 receptor and Gαz by measuring the inhibition of adenylyl cyclase activity [1].Animal Administration: HT–2157 (SNAP 37889) is prepared in 5% DMSO and 1% HMC in saline [2].[2]Rat [2]The effect of HT–2157 on locomotor activity is examined using adult male iP rats (n=12; 418–467 g). Locomotor activity is tested to ensure that any potential change in anxiety or ethanol consumption in subsequent tests is not due to any sedative property of the drug. Rats are habituated to the locomotor cells (26×26×40 cm) for 60 minute sessions which are conducted daily for threeconsecutive days. To minimise the effect of habituation during the treatment phase, rats are divided into two even groups which received a single injection of either HT–2157 (30 mg/kg, i.p.) or vehicle (1 ml/kg i.p.) 30 min prior to being placed in the locomotor cells for a 60 minute session. The following day the treatments are reversed so that all rats received treatment with both the vehicle and SNAP 37889 compound. Movements in terms of number of moves, move time (s) and total distance travelled (cm), are recorded automatically using TruScan 2.0 software.References:Product Name:HT–2157Cat. No.:HY-100717CAS No.:303149-14-6Molecular Formula:C 21H 13F 3N 2O Molecular Weight:366.34Target:Neuropeptide Y Receptor Pathway:GPCR/G Protein Solubility:DMSO: ≥ 29 mg/mL[1]. Swanson CJ, et al. Anxiolytic– and antidepressant–like profiles of the galanin–3 receptor (Gal3) antagonists SNAP 37889 and SNAP 398299. Proc Natl Acad Sci U S A. 2005 Nov 29;102(48):17489–94.[2]. Ash BL, et al. The galanin–3 receptor antagonist, SNAP 37889, reduces operant responding for ethanol in alcohol–preferring rats. Regul Pept. 2011 Jan 17;166(1–3):59–67.Caution: Product has not been fully validated for medical applications. For research use only.Tel: 609-228-6898 Fax: 609-228-5909 E-mail: tech@Address: 1 Deer Park Dr, Suite Q, Monmouth Junction, NJ 08852, USA。

双荧光素酶报告基因检测试剂盒

双荧光素酶报告基因检测试剂盒

注意事项:
1) Fassay Buffer I和Fassay Substrate I应避免反复冻熔,可分装成合适体积分次使用。 Rassay Substrate II溶液应盖严存放,避免蒸发。配制好未用完的Fassay Reagent I和 Rassay Reagent II可在-20℃保存1月左右。
自动发光测定:
配制好的Fassay Reagent I和Rassay Reagent II置于测定仪内并连接好对应管道,Fassay Reagent I接第一注射管道,Rassay Reagent II接第二注射管道。各待测样品20 μl分别加 入测定管/板孔底部,启动自动测量程序。记录Firefly luciferase和Ranilla luciferase的发光 单位(RLU)。
测定前,在室温待Fassay Buffer I、Fassay Substrate I和Rassay Buffer II溶化,混匀(注意 避光)。按20/1比例用Fassay Buffer I稀释Fassay Substrate I,按50/1比例用Rassay Buffer II 稀释Rassay Substrate II,分别配制所需体积的Fassay Reagent I和Rassay Reagent II(注意 避光)。
2) 细胞裂解液一般在当天测定。如需隔日测定,应将样品于-20℃保存。长期保存应 在-80℃。测定样品量可为10~30μl个样品的两种试剂加入时间间 隔一致。
4) Rassay Reagent II可用于直接测定样品的Ranilla luciferase。需要注意的是,Rassay Reagent II直接测量的RLU要比双荧光素酶顺序检测获得的RLU高一些(反应体积 等因素的影响)。

EPZ015666_DataSheet_MedChemExpress

EPZ015666_DataSheet_MedChemExpress

Inhibitors, Agonists, Screening Libraries Data SheetBIOLOGICAL ACTIVITY:EPZ015666 is an orally available inhibitor of PRMT5 enzymatic activity in biochemical assays with IC 50 of 22 nM and broad selectivity against a panel of other histone methyltransferases.IC50 & Target: IC50: 22 nM (PRMT5)[1]In Vitro: Treatment of MCL cell lines with EPZ015666 leads to inhibition of SmD3 methylation and cell death, with IC 50 values in the nanomolar range [1]. EPZ015666, a potent peptide–competitive and SAM–cooperative inhibitor with >10,000–fold specificity againstPRMT5 relative to other methyltransferases [2].In Vivo: EPZ015666 is orally bioavailable and amenable to in vivo studies. We performed 21–d efficacy studies in severe combined immunodeficiency (SCID) mice bearing subcutaneous Z–138 and Maver–1 xenografts, with twice–daily (BID) oral dosing on four dose groups: 25, 50, 100 and 200 mg per kilogram of body weight (mg/kg). After 21 d of continuous dosing, animals areeuthanized, and blood and tissues are analyzed to determine the relationship between methyl–mark pharmacodynamics andtumor–growth inhibition (TGI). EPZ015666 showed dose–dependent exposure and TGI after 21 d in both MCL models. Tumors in all EPZ015666 dose groups measured on day 21 showed statistically significant differences in weight, volume and tumor growth compared to vehicle–treated tumors. Dosing at 200 mg/kg BID induced tumor stasis in Z–138 cells, with >93% TGI after 21 d,whereas Maver–1 cells showed >70% TGI. Additionally, a third MCL xenograft is tested using the Granta–519 cell line, afast–growing model that reached endpoint on day 18 and showed dose–dependent efficacy with 45% TGI in the 200 mg/kg group.EPZ015666 is well tolerated in all three models, with minimal bodyweight loss in the 200 mg/kg dose group and no other clinical observations [1].PROTOCOL (Extracted from published papers and Only for reference)Kinase Assay:[1]EPZ015666 is serially diluted threefold from 1,000 to 0.051 nM and spotted into a 384–well polypropyleneV–bottom microplate. 3H–SAM is serially diluted twofold in assay buffer for a seven–point dilution series with a top concentration of 700 nM (final assay concentration). Reactions are initiated by the addition of 4 nM enzyme and 40 nM peptide (final assayconcentrations for both). Reactions are incubated for 60 min and quenched by the addition of 10 μL per well of 600 μM unlabeled SAM in assay buffer (final assay concentration). For the peptide competition, EPZ015666 is serially diluted threefold from 1,000 to 0.051 nM and spotted into a 384–well polypropylene V–bottom microplate. Peptide is serially diluted twofold in assay buffer for a seven–point dilution series with a top concentration of 480 nM (final assay concentration). Reactions are initiated by the addition of 4 nM enzyme and 75 nM 3H–SAM (final assay concentrations for both). Reactions are incubated for 60 min, and the reactions are quenched by the addition of 10 μL per well of 600 μM unlabeled SAM in assay buffer (final assay concentration)[1].Cell Assay: EPZ015666 is dissolved in DMSO and stored, and then diluted with appropriate medium (final DMSO 0.2%)before use [1].[1]Cultured cells in linear/log–phase growth are split to a seeding density of 2×105 cells/mL in 2–20 mL of media,depending on the yield required at the end of the growth period. Compound is diluted in DMSO and added to each culture vesselProduct Name:EPZ015666Cat. No.:HY-12727CAS No.:1616391-65-1Molecular Formula:C 20H 25N 5O 3Molecular Weight:383.44Target:Histone Methyltransferase Pathway:Epigenetics Solubility:DMSOwith a final DMSO concentration of 0.2%. Cells are allowed to grow for 96 h undisturbed. At the conclusion of each treatment period, cells are harvested by centrifugation (5 min, 1,200 rpm), and cell pellets are rinsed once with PBS before being frozen on dry ice pending further processing. Long–term proliferation assays are performed on all MCL lines, with slight adjustments to initial seeding densities, depending on growth characteristics for each cell line. All assays are carried out for 12 d[1].Animal Administration: EPZ015666 is formulated in 20% N–N–dimethylacetamide in water (Mice)[1].[1]Mice[1]Male CD–1 mice (25–40 g; n=6, with 3 per time point) are treated with a single dose of EPZ015666 at 2 mg/kg by intravenoustail–vein injection and 10 mg/kg by oral gavage administration, with both doses formulated in 20% N–N–dimethylacetamide in water. Animals are fasted overnight and weighed before dose administration on the day of dosing. Approximately 30 μL ofblood are taken from animals by submandibular or retro–orbital bleeding at pre–specified time intervals (seven time points). For the last time point (24 h), samples are collected via cardiac puncture while the animals are under anesthesia (70% CO2:30% O2). Blood samples are transferred into K2–EDTA tubes and placed on wet ice before centrifugation at 4°C (3,000g, 15 min) to obtain plasma within 30 min after sample collection. Plasma samples are stored at -70±10°C before protein precipitation and LC–MS/MS analysis. We constructed standard calibration curves by analyzing a series of control plasma aliquots containing 100 ng/mL labetalol as an internal standard and 1–3,000 ng/mL EPZ015666. Four levels of quality control are also included in the analysis (3–2,400 ng/mL EPZ015666). Data are analyzed using Phoenix WinNonlin 6.2.1.References:[1]. Chan–Penebre E, et al. A selective inhibitor of PRMT5 with in vivo and in vitro potency in MCL models. Nat Chem Biol. 2015 Jun;11(6):432–7.[2]. Kryukov GV, et al. MTAP deletion confers enhanced dependency on the PRMT5 arginine methyltransferase in cancer cells. Science. 2016 Mar 11;351(6278):1214–8.Caution: Product has not been fully validated for medical applications. For research use only.Tel: 609-228-6898 Fax: 609-228-5909 E-mail: tech@Address: 1 Deer Park Dr, Suite Q, Monmouth Junction, NJ 08852, USA。

SKIDA 4 Plus 多通道注射器分析仪说明书

SKIDA 4 Plus 多通道注射器分析仪说明书

Key features• Tests up to four infusion pumps simultaneously• Compatible with virtually any type of infusion device • Instantaneous and average flow measurement • Occlusion pressure measurements to 45 psi • Single- and dual-flow (piggyback) testing• Full PCA testing (bolus volume, lockout time, and automated external triggering)• Autostart mode enables unit to begin testing only when fluid is detected • On-board graphing of pressure and flow• Built-in memory to save test results for printing or downloading to computer • Hydrograph graphical software to control unit and display results via PC • Automated testing through Fluke Biomedical medTester 5000C (US only)Technical DataIDA 4 Plus Multi-Channel Infusion Device Analyzer maximizes productivity with multiple, independent channels for testing up to four infusion pumps at once.The device measures instantaneous flow, average flow, occlusion pressure, and analyzes patient-control analgesia (PCA) units. An optional PCA trigger box provides automated PCA pump control, allowing technicians to set up tests and walk away.An autostart feature simplifies syringe pump testing or other tests that have long startup times.With built-in memory, the IDA 4 Plus records test results internally and provides easy-to-read test-result graphs right on the analyzer’s screen. The display is so large numbers can be read from across the room.Additionally, the IDA 4 Plus comes withHydrograph PC software for creating full-color graphs and reports. For automated testing, the IDA 4 Plus is compatible with Fluke Biomedical’s medTester 5000C (US only).ElsoPhilipsService,Jilemnického2;91101Trenèín;tel:+421326582410,7431690;fax:+421326582592;email:**************;web:www.fluke.skTechnical specificationsFlow-rate measurementTechnique: Calculated by mea-suring a volume over timeRange: 0.5 ml/hr to 1000 ml/hr Accuracy: 1 % of reading ± 1 LSD for flows of 16 ml/hr to 200 ml/hr for volumes over20 ml; otherwise, 2 % of reading ± 1 LSD after delivery of 10 ml Volume measurementTechnique: Volume measured directly by the transducer in minimum sample sizes of 60 µl Range: 0.06 ml to 9999 ml Accuracy: 1 % of reading ± 1 LSD for flows of 16 ml/hr to 200 ml/hr for volumes over20 ml; otherwise, 2 % of reading ± 1 LSD after delivery of 10 ml PCA bolus measurementTechnique: Volume is measured directly by the transducer in minimum bolus volumes of 0.5 ml. The measurement is made with a continuous rate between 0 ml/hr and 30 ml/hr. The bolus flow rate should be at least four times the basal flow rate for reliable detection of boluses.Minimum bolus volume: 0.5 ml Accuracy: See volume measurementPressure measurementTechnique: Direct occlusion of the infusion line and measure-ment of pressure prior to the glass transducerRange: 0 psi to 45 psi and equivalents in mmHg and kPa Accuracy: 1 % of full scale ± 1 LSDBack pressure: -100 mmHg to 300 mmHgElectrical specificationsSupply voltage: Autoswitching90 V ac to 260 V acSupply frequency: 50 Hz to 60 HzSupply power: < 30 VAFuse: 20 mm 250 V, 1 A (T) (slow blow)Earth leakage current: < 1 mA in single fault condition Environmental conditionsOperating temperature: 15 ˚C to 30 ˚C (59 ˚F to 86 ˚F)2 Fluke Biomedical IDA 4 Plus Multi-Channel Infusion Device AnalyzerStorage temperature: 0 ˚C to 40 ˚C (32 ˚F to 104 ˚F) at 85 % RH or less for storage [Do not leave for more than 48 hours at -20 ˚C (-4 ˚F)]General informationDimensions (WxDxH): 19.05 cm x 18.11 cm x 30.18 cm (7.5 in x 7.2 in x 11.9 in)Weight: 5 kg (11 lb)“demand” signals. With the IDA 4 Plusconnected to the Trigger Box, it will measure,display, and record essential PCA pumpparameters to show bolus and flow characteristics.The PCA Trigger Box can also be used to test the “nurse call” interface of PCA Pumps. Simulate “nurse calls” and look for the response indicated by makers in the flow graph.HydroGraph™Graphics SoftwareUse the moving colorvisual advantage ofHydroGraph to troubleshootup to four infusion pumpsat once. Data is takendirectly off the transducerand transmitted to HygroGraph.The flowing graphs provide anelectronic means to display, store, and recall flow patterns for comparison at a later date. Each test window can display instantaneous and average flow rates, cumulative, and bolus volumes; and occlusion pressure.IDA 4 Plus Multi-Channel Infusion Device Analyzer Fluke Biomedical 3Ordering informationModelsIDA 4 Plus One-Channel Infusion Device Analyzer 2250063 IDA-4P/1-US120V (US)2394575 IDA-4P/1-AUS250V(Australia)2394582 IDA-4P/1-DEN250V(Denmark)2394594 IDA-4P1-SHK250V (Shuko)2394608 IDA-4P/1-ISR250V (Israel)2394613 IDA-4P/1-ITAL250V (Italy)2394624 IDA-4P/1-IND250V (India)2394636 IDA-4P/1-SWZ250V(Switzerland)2394649 IDA-4P/1-UK250V (UK) IDA 4 Plus Two-Channel Infusion Device AnalyzerFull testing for up to two infusion pumps simultaneously2250088IDA-4P/2-US120V (US)2394651IDA-4P/2-AUS250V(Australia)2394660IDA-4P/2-DEN250V(Denmark)2394672IDA-4P/2-SHK250V (Shuko)2394685IDA-4P/2-ISR250V (Israel)2394697IDA-4P/2-ITAL250V (Italy)2394703IDA-4P/2-IND250V (India)2394715IDA-4P/2-SWZ250V(Switzerland)2394726IDA-4P/2-UK250V (UK)IDA 4 Plus Three-ChannelInfusion Device AnalyzerFull testing capability for up to threeinfusion pumps simultaneously2250109IDA-4P/3-US120V (US)2394732IDA-4P/3-AUS250V(Australia)2394744IDA-4P/3-DEN250V(Denmark)2394759IDA-4P/3-SHK250V (Shuko)2394767IDA-4P/3-ISR250V (Israel)2394771IDA-4P/3-ITAL250V (Italy)2394780IDA-4P/3-IND250V (India)2394798IDA-4P/3-SWZ250V(Switzerland)2394800IDA-4P/3-UK250V (UK)IDA 4 Plus Four-ChannelInfusion Device AnalyzerFull testing capability for up to fourinfusion pumps simultaneously2250127IDA-4P/4-US120V (US)2394817IDA-4P/4-AUS250V(Australia)2394821IDA-4P/4-DEN250V(Denmark)2394839IDA-4P/4-SHK250V (Shuko)2394842IDA-4P/4-ISR250V (Israel)2394856IDA-4P/4-ITAL250V (Italy)2394863IDA-4P/4-IND250V (India)2394874IDA-4P/4-SWZ250V(Switzerland)2394888IDA-4P/4-UK250V (UK)Standard accessories2213506Electronic User Manual andHydroGraph Software2238626Serial Communication Cable221723120 ml priming syringe2391750Luerlock-3 way (one foreach channel)2238909 5 foot plastic drain lineXXXXXXX Power cord (countryspecific)Optional accessories2245061 External Mini-Keyboard,83-Key with PS/2Connector and AT Adapter2238072 Parallel Printer Cable(D25M-36M)2209703 PCA Trigger/Nurse Call Box2248899 Printer, Seiko DPU-414-30B(120 V power supply)(additional purchaserequired: Parallel PrinterCable, p/n 2238072)2399531 Printer, Seiko DPU-414-30B(220 V power supply)(additional purchaserequired: Parallel PrinterCable, p/n 2238072)2235375 Printer (120 V powersupply)2235382 Printer (220 V powersupply)2200102 Interface Cable, medTesterto IDA 4 Plus (withoutwedge adapter) (RS-232;female DB25 to female DB9)2201042 Interface Cable, medTesterto IDA 4 Plus (with wedgeadapter) (RS-232; femaleDB9 to female DB25)2245092 Barcode Scanner (withlong-reach coil cable withY connector for keyboardattachment)2238626 Null Modem Cable (femaleDB9 to female DB9)Fluke Biomedical.Better products. More choices. One company.Fluke Biomedical 6045 Cochran RoadCleveland, OH 44139-3303 U.S.A.Fluke Biomedical Europe Science Park Eindhoven 5110, 5692EC Son, The NetherlandsFor more information, contact us:In the U.S.A. (800) 850-4608 or Fax (440) 349-2307In Europe/M-East/Africa +31 40 267 5200 or Fax +31 40 267 5436From other countries +1 (440) 248-9300 or Fax +1 (440) 349-2307Email:*************************Web access: ©2006-2008 Fluke Biomedical. Specifications subject to change without notice. All OEM trademarks are implied. Printed in U.S.A. 3/2008 2804429 D-EN-N Rev Briešenia na presné meranie™Elso Philips Service; tel: +421 32 6582410email: ************; web: www.elso.sk。

QPCR及QRT-PCR系列产品

QPCR及QRT-PCR系列产品

Invitrogen的ICFC系列产品促销1.QPCR及QRT-PCR系列产品Invitrogen公司专门为中国客户提供的定量PCR试剂盒,结合了 UDG 防止残余污染技术和SYBR® Green I 荧光染料(存在于SYBR® Green I荧光定量PCR试剂盒中),在美国接受了严格的质量监控,可提供极高灵敏度的目的序列定量检测,线性剂量低,反应浓度范围很大。

qPCR Supermix-- 即用型反应剂,专为高特异性、实时定量DNA扩增设计UDG-- 防止携带污染物,减少克隆片段假阳性结果ROX参考染料-- 适用ABI仪器的校正染料产品信息活动时间:即日起至2009年4月30日2.Gibco南美胎牛血清即日起凡优惠价¥1780购买Gibco胎牛血清500ml(目录号:C2027050)即可获赠送价值¥250现金抵用券。

您可以凭现金抵用券在英韦创津公司购买任何商品,此券有效期至2009年5月31日。

产品信息活动时间:即日起至2009年4月30日独特的采集方式:GIBCO采用无菌心脏穿刺的方式采血原装直送,避免污染:原产地采集、加工、检测、包装。

完善的质控:采集、处理、检测、运输等环节都有文件和证书。

3.Invitrogen TA Cloning克隆产品专门用于克隆Taq聚合酶扩增的PCR产物。

采用pCR载体,能产生80%以上的重组产物,90%以上重组产物都包含插入片段。

产品信息活动时间:即日起至2009年5月31日附:pCR载体优点及图谱:3’-T突出端可直接连接Taq扩增的PCR产物可选择T7或T7和Sp6启动子进行体外RNA转录和测序侧向EcoRⅠ位点的通用多接头位点方便了插入片段的切离可以选择卡那霉素或氨苄青霉素进行筛选非常简便的蓝/白克隆筛选具有M13正向和反向引物位点,方便测序4.GIBCO液体培养基系列产品创立近50年的历史,品质优秀,产品种类丰富;为了中国用户利益,特建立国内生产线;所有产品,从原材料到生产全部按照GIBCO质量标准进行,每批均送抵美国公司总部质检合格后,才在国内销售。

普利莱基因技术有限公司线粒体 胞浆制备试剂盒C1260说明书

普利莱基因技术有限公司线粒体 胞浆制备试剂盒C1260说明书

线粒体/胞浆制备试剂盒C1260描述:线粒体/胞浆制备试剂盒(Mitochondria Isolation Kit)用于从组织或培养细胞中分离线粒体和细胞胞浆成分。

加入分离溶液,匀浆破碎组织细胞,经过数次800g和12000g离心,在60分钟内即可分离出完整的线粒体和胞浆成分。

制备的线粒体具有很高的生物学活性,可进行各种功能研究如酶学测定,更可用于Western Blot、2D-胶、线粒体蛋白或DNA提取、蛋白质组学等研究。

严格按照说明操作,总是能制备获得高纯度线粒体。

一篇方法学研究论文发现,用普利莱试剂盒制备线粒体的得率、活性、纯度优于蔗糖密度梯度离心法和Invotrogen/Pierce线粒体提取试剂盒方法。

适用:从组织、培养细胞制备高纯度线粒体,同时分离细胞胞浆成分。

组成:Mito Solution 100 ml for 50 次制备200 ml for 100 次制备储存:−20 ºC 12个月有效操作步骤:以下所有操作均在4 ºC进行1.组织匀浆:100~200 mg新鲜组织如肝、脑、肾、心肌等,剪为0.5cm2碎块放入小容量玻璃匀浆器内。

估计组织块总体积。

加入1.5 ml冰预冷的Mito Solution。

用间隙严紧的研杵上下研磨组织20次。

培养细胞匀浆:800 × g 5 min离心收集细胞。

单次提取需2-5 × 107个细胞。

加入1.5 ml冰预冷Mito Solution 重悬细胞,将细胞悬液转移到小容量玻璃匀浆器内,用间隙严密的研杵研磨细胞30次。

2.将匀浆液转移到离心管中,800 × g ,4 ºC离心5 min。

(胞核、膜碎片、未裂解细胞等在管底,弃去)3.收集上清液并转移到新的离心管。

再次800 × g 离心5 min at 4 ºC,弃沉淀。

4.将上清液转移到新的离心管。

10,000 × g 离心10 min 4 ºC。

IWP-L6_Porcupine抑制剂_1427782-89-5_Apexbio

IWP-L6_Porcupine抑制剂_1427782-89-5_Apexbio
产品说明书
化学性质
产品名: Cas No.: 分子量: 分子式:
IWP-L6 1427782-89-5 472.58 C25H20N4O2S2
产品名: IWP-L6 修订日期: 6/30/2016
化学名: SMILES: 溶解性: 储存条件: 一般建议:
运输条件:
2-[(4-oxo-3-phenyl-6,7-dihydrothieno[3,2-d]pyrimidin-2-yl)sulfanyl]N-(5-phenylpyridin-2-yl)acetamide
Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request
生物活性
靶点 :
Stem Cell
信号通路:
Wnt/β-catenin
产品ቤተ መጻሕፍቲ ባይዱ述:
C1CSC2=C1N=C(N(C2=O)C3=CC=CC=C3)SCC(=O)NC4=NC=C(C=C4)C5 =CC=CC=C5
>22.45mg/mL in DMSO
Store at -20&deg; C
For obtaining a higher solubility , please warm the tube at 37°C and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20°C for several months.
特别声明
产品仅用于研究,
不针对患者销售,望谅解。 每个产品具体的储存和使用信息显示在产品说明书中。ApexBio 产品在推荐的条件下是稳定 的。产品会根据不同的推荐温度进行运输。许多产品短期运输是稳定的,运输温度不同于长 期储存的温度。我们确保我们的产品是在保持试剂质量的条件下运输的。收到产品后,按照 产品说明书上的要求进行储存。

透析免疫沉淀试剂盒(50 次反应) (Pierce 交联 IP 试剂盒)说明书

透析免疫沉淀试剂盒(50 次反应) (Pierce 交联 IP 试剂盒)说明书

说明书Pierce®交联 IP(免疫沉淀)试剂盒26147 2134.8货号描述26147 Pierce 交联 IP 试剂盒,包含足够进行 50 次免疫沉淀反应的试剂(每次使用 10 μL 树脂用于固定化抗体)试剂盒组分:Pierce 蛋白 A/G 加强型琼脂糖树脂,0.55 mL 固相树脂以 50%的浆液形式提供(例如,100 μL 50%的浆液含有 50 μL 固相树脂)。

20X交联缓冲液,25 mL,使用时稀释至1X,即为:0.01 M 磷酸钠盐缓冲液,0.15 M NaCl;pH 7.2 溶液DSS (辛二酸二琥珀酰亚胺酯),No-Weigh™(免称重)式,8X2mg 微管包装IP 裂解/洗涤缓冲液,2 X 50 mL,0.025 M Tris,0.15 M NaCl,0.001 M EDTA,1% NP-40,5%甘油;pH 7.4100X条件缓冲液,5 mL,中性 pH 缓冲液20XTris-缓冲盐溶液,25 mL,使用时稀释至1X,即为 0.025 M Tris,0.15 M NaCl;pH 7.2溶液洗脱缓冲液,50 mL,pH 2.8,含有伯胺非还原型上样缓冲液,(5X),5 mL,0.3M Tris•HCl,5% SDS,50%甘油,泳道标记示踪染料;pH 6.8Pierce 离心柱-带螺旋盖,100 个柱子,且包含相应配件微量离心收集管,2 mL,100 个微量离心样品管,1.5 mL,50 个Pierce 对照琼脂糖树脂(4%交联琼脂糖珠),2 mL 固相树脂以 50%的浆液形式提供(例如,100 μL 50%的浆液含有 50 μL 固相树脂)储存:收到试剂盒后将其储存于 4°C 。

DSS 置于 4°C 干燥保存。

试剂盒于室温运输。

产品简介Thermo Scienti c Pierce 交联 IP 试剂盒通过将抗体共价交联于蛋白 A/G 树脂从而高效地实现抗原免疫沉淀反应。

银耳多糖对人软骨细胞的增殖效应和抗炎作用

银耳多糖对人软骨细胞的增殖效应和抗炎作用

谭敏颖,戴川景,卢学敏,等. 银耳多糖对人软骨细胞的增殖效应和抗炎作用[J]. 食品工业科技,2024,45(1):1−8. doi:10.13386/j.issn1002-0306.2023060077TAN Minying, DAI Chuanjing, LU Xuemin, et al. Proliferation and Anti-inflammatory Effects of Tremella fuciformis Polysaccharide on Human Chondrocytes[J]. Science and Technology of Food Industry, 2024, 45(1): 1−8. (in Chinese with English abstract). doi:10.13386/j.issn1002-0306.2023060077· 特邀主编专栏—食品中天然产物提取分离、结构表征和生物活性(客座主编:杨栩、彭鑫) ·银耳多糖对人软骨细胞的增殖效应和抗炎作用谭敏颖1,2,戴川景1,卢学敏1,王毅刚1,关 磊2,程 勇2, *(1.浙江理工大学生命科学与医药学院,浙江杭州 310018;2.浙江天草生物科技股份有限公司,浙江湖州 313399)摘 要:目的:骨关节炎(Osteoarthritis ,OA )是一种常见的慢性关节性疾病,本研究旨在探究银耳多糖对骨关节炎细胞模型人软骨细胞T/C-28a2的增殖效应和抗炎作用。

方法:通过MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide )和结晶紫染色实验检测银耳多糖对T/C-28a2细胞增殖活力和细胞毒性的影响;用脂多糖(Lipopolysaccharide ,LPS )处理T/C-28a2细胞建立骨炎症模型,酶联免疫吸附测定(Enzyme-linked immunosorbent assay ,ELISA )检测药物处理后细胞白介素-6(Interleukin-6,IL-6)的表达;利用蛋白免疫印迹(Western blot )检测药物处理后相关骨保护因子和炎症因子的表达;通过ROS 活性氧释放实验检测药物对细胞的氧化应激水平和抗炎症反应。

Expression, Purification and Crystallization of

Expression, Purification and Crystallization of

Expression, Purification and Crystallization of the Mycobacterium Tuberculosis HSP16.3 Molecular Chaperone Background of Mycobacterium Tuberculosis HSP16.3HSP16.3, a 16.3 kDa protein from Mycobacterium Tuberculosis, was originally identified as a prominent antigen (Kingston et al., 1987). During the stationary phase, HSP16.3 is maximally expressed and becomes a main protein of the latent phase (Yuan et al., 1996). Previous studies showed that HSP16.3 can make the cell structure stable and prevent stationary Mycobacterium Tuberculosis from autolysing (Cunningham et al., 1998). In previous studies, HSP16.3 was found as one of theα-crystallin-related small heat shock proteins (sHSP) with molecular chaperone activity. Experiments in vitro revealed that HSP16.3 can suppress the thermal aggregation of citrate synthase at 39.5˚C, without consumption of A TP (Chang et al., 1996).Now the Mycobacterium Tuberculosis HSP16.3 gene was cloned to the plasmid pSTE-HSP16.3, and transformed to E.Coli. BL21(DE3) strain.Material and MethodExpressionThings to have ready before Starting.-Plate or glycerol culture-Sterile LB 25ml in a 50mL shaker flasker, 250ml in a 500mL shaker flasker, all together autoclaved, antibiotic added afterword.- antibiotic and sterile water- TipsPrepare the LB and autoclave:Fomula of the LB medium for 1 Liter:Bacto Tryptone (BT) 10 gBacto Y east Extract (BYE) 10 gNaCl 10gThe LB medium, dd H2O and the tips all together autoclaved at 121 ˚C for 20 minutes.Method:1 Innoculate 25 ml LB Medium ( containing 100 ug) and grow culture overnight(37˚C, 200rpm).2 Next morning inoculate 250 ml prewarmed LB Medium ( containing 100 ug) with the 25 ml overnight culture and grow at 37 ˚C, 200rpm, HSP16.3 was overexpressed in soluble form intracellularly without IPTG induction.3 Incubate the Culture for 10 hours before havesting the cell at 4000 g for 20 minutes.4 Resuspend the cell pellet in 30 ml Butter A and freeze the Sample in -80˚C refigerator.PurificationDE52 Ion-Exchange columnThings to have ready before Starting.-Butter A: 50 mM Imidazole pH 6.5 (1 liter)-Butter B: 50 mM Imidazole pH 6.5 , 300mM NaClall together Fitrate with 0.2 um membrane.- DE52 medium , column ,Gradient maker, UV-monitor and Fractioner- TipsMethod:1 Thaw the cell pellet and vortex .2 Add 0.4ml 100 mM PMSF and sonicate (400kw, 4s-6s 50 cycle* 5 )3 Centrifuge 15000 rpm, 30 minutes to pellet debris4 Transfer supernatant to a 50 ml conicale tube and discard the pellet.5 The supernatant dilute to 50 ml with Buffer A and then load to DE52 ion-exchange columns (20ml), which was pre-equibrated with 100ml Buffer A. And then wash the unbound proteins with 100 ml Buffer A.6 Elute the protein with a linear gradient : 200ml buffer A plus 200ml buffer B, 2ml/min, 6ml each fraction.7 Run 15% SDS-PAGE to determine the HSP16.3 peak.Desalting by dialysis1 Preparation of the dialysis tubeCut the tube in a suitable length (20-30 cm)Boil the tube in solution containing 10 mM NaHCO3 for a few minutes.Boil the tube in solution containing 10 mM EDTA for a few minutes.Rasin the tube with de-ion water2 Pool the HSP16.3 peak and dialysis the Sample against 1000ml Buffer A for more than 6hours.Q-Separose (HP) Ion-Exchange Column1 load the sample to Q-Separose (HP) Ion-Exchange column (20ml), which was pre-equibrated with 100ml Buffer A. And then wash the unbound proteins with 100 ml Buffer A.2 Elute the protein with a linear gradient : 200ml buffer A plus 200ml buffer B, 2ml/min, 6ml each fraction.3 Run 15% SDS-PAGE to determine the purity of the HSP16.3 peak.Gel filtration ColumnThe HSP peak was a final volumn 0.3ml and then run though a Superdex75 (HR, 10/30mm) gel filtration column in 150mM NaCl and 5mM Imdazole, pH6.5. Crystallization1 The purified HSP16.3 was solvent-exchanged to water and concentrated to 20mg/ml before crystallization trails (Bradford). All the crystallization trials were carried out using the hanging-drop vapor-diffusion method at 291K: drops consisted of2 microlitres of HSP16.3 protein solution plus 2 microlitres of the precipitant. The drops were equilibrated against 0.2 ml precipitant at room temperature. The crystallization conditions were investigated with a PEG4000 Kit.Result and discussionThe purity of the final HSP16.3 was over 95% by SDS-PAGE. The crystallization trials of HSP16.3 yielded Cubic crystals with a size of 0.8*0.8*0.6mm in a few days.20040060080010001200mAUBuffer Tris-HCL pH 8.5 Precipitant PEG 4000 MethodV apor Diffusion Temperature 293 K Size0.8*0.8*0.6mmReferencesChang Z., Primm, T.P., Jakana J., Lee H. I., Serysheva I., Chiu W., Gilber H. F., Quiocho F. A., (1996) J Biol Chem 271:7218-7223Cunningham A. F., Spreadbury C. L., (1998) J. Bacteriol. 184:801-808Kingston A. E., Salgame P. R., Mitchison N.A., Colston M. J. (1987) Infect. Immun 55,3149-3154Yuan Y., Crane D. D., Barry C. E. III (1996) J Bacteriol178: 4484-4492。

WB及IHC实验所用试剂汇表[1]

WB及IHC实验所用试剂汇表[1]
CW0043
500ml
曝光
ECL
CW0048
25ml
超灵敏ECL
CW0049
25ML
显影粉
CW0062
10包(10*250ml)
定影液
CW0063
10包(10*250ml)
X-光片
CW0061
100张
X-暗盒
CW0060
1个
膜再生液
CW0056
100ML
显色
DAB显色试剂盒
CW0125
20ML
BCIP/NBT碱性磷酸酶显色试剂盒
鼠源免疫组化试剂盒
CW0119
3ml/18ml
198元/998元
Cw2068
3ml/18ml
258元/1098元
兔鼠通用性
Cw0120
3ml/18ml
258元/1198元
Cw2069
3ml/18ml
298元/1298元
复染
改良型苏木素CW0127
封片
中性树胶
CW0136
水性封片剂
CW0137
抗体稀释液
CW0043
500ML
多种内参抗体(CW0096-CW0101)
多种二抗(CW0102-CW0176)
IHC系列产品
名称
货号
规格
价格
抗原修复
柠檬酸缓冲液CW0128
EDTA缓冲液CW0129
免疫组化试剂盒
兔源免疫组化试剂盒
CW0118
3ml/18ml
198元/998元
CW2035
3ml/18ml
498元/1098元
一抗稀释液
CW0133
二抗稀释液

Y-27632_DataSheet_MedChemExpress

Y-27632_DataSheet_MedChemExpress

Inhibitors, Agonists, Screening Libraries Data SheetBIOLOGICAL ACTIVITY:Y–27632 is an ATP–competitive inhibitor of ROCK–I and ROCK–II , with K i of 220 nM and 300 nM for ROCK–I and ROCK–II , respectively.IC50 & Target: Ki: 220/300 nM (ROCK–I/II)[1]In Vitro: Y–27632 inhibits the ROCK family of kinases 100 times more potently than other kinases including protein kinase C,cAMP–dependent kinase and myosin light chain kinase. Y–27632 prolongs the lag time and delays the appearance of BrdU–labeled cells in a concentration–dependent manner, delays of about 1 and 4 h are noticed in the Swiss 3T3 cells treated with 10 and 100 μM Y–27632, respectively [1]. Y–27632 promotes neuronal differentiation of adipose tissue–derived stem cells (ADSCs). Compared to 1.0and 2.5 μM Y–27632 induced groups, percentages of neuroal–like cells achieved a peak in the 5.0 μM Y–27632 induced group [2].In Vivo: Y–27632 (5 and 10 mg/kg) significantly prolongs the onset time of myoclonic jerks when compare with saline group.Y–27632 (5 and 10 mg/kg) significantly prolongs the onset time of clonic convulsions when compare with saline group [3].Treatment with Dimethylnitrosamine (DMN) causes a significant decrease in rat body and liver weight (DMN–S group) compared with control animals (S–S group). Oral Y27632 (30 mg/kg) essentially prevents this DMN–induced rat body and liver weight loss (DMN–Y group)[4].PROTOCOL (Extracted from published papers and Only for reference)Kinase Assay:[1]Recombinant ROCK–I, ROCK–II, PKN, or citron kinase is expressed in HeLa cells as Myc–tagged proteins by transfection using Lipofectamine, and is precipitated from the cell lysates by the use of 9E10 monoclonal anti–Myc antibodycoupled to G protein–Sepharose. Recovered immunocomplexes are incubated with various concentrations of [32P]ATP and 10 mg of histone type 2 as substrates in the absence or presence of various concentrations of either Y–27632 or Y–30141 at 30°C for 30 min in a total volume of 30 μL of the kinase buffer containing 50 mM HEPES–NaOH, pH 7.4, 10 mM MgCl 2, 5 mM MnCl 2, 0.02% Briji 35, and 2 mM dithiothreitol. PKCa is incubated with 5 μM [32P]ATP and 200 μg/mL histone type 2 as substrates in the absence or presence of various concentrations of either Y–27632 or Y–30141 at 30°C for 10 min in a kinase buffer containing 50 mM Tris–HCl,pH 7.5, 0.5 mM CaCl 2, 5 mM magnesium acetate, 25 μg/mL phosphatidyl serine, 50 ng/mL 12–O–tetradecanoylphorbol–13–acetate and 0.001% leupeptin in a total volume of 30 μL. Incubation is terminated by the addition of 10 μL of 43 Laemmli sample buffer.After boiling for 5 min, the mixture is subjected to SDS–polyacrylamide gel electrophoresis on a 16% gel. The gel is stained withCoomassie Brilliant Blue, and then dried. The bands corresponding to histone type 2 are excised, and the radioactivity is measured [1]. Cell Assay: Y–27632 is dissolved in water and stored [1].[1]HeLa cells are plated at a density of 3×104 cells per 3.5–cm dish. The cells are cultured in DMEM containing 10% FBS in the presence of 10 mM Thymidine for 16 h. After the cells are washed with DMEM containing 10% FBS, they are cultured for an additional 8 h, and then 40 ng/mL of Nocodazole is added. After 11.5 h of theNocodazole treatment, various concentrations of Y–27632 (0–300 μM), Y–30141, or vehicle is added and the cells are incubated for another 30 min [1].Animal Administration: Y–27632 is dissolved in 0.9% NaCl (saline) (Mice)[3].Product Name:Y–27632Cat. No.:HY-10071CAS No.:146986-50-7Molecular Formula:C 14H 21N 3O Molecular Weight:247.34Target:ROCK; ROCK; ROCK Pathway:TGF–beta/Smad; Stem Cell/Wnt; Cell Cycle/DNA Damage Solubility:DMSO: ≥ 32 mg/mLY–27632 is dissolved in saline (final concentration 2%) (Rat)[4].[3][4]Mice[3]Male, inbred Swiss albino mice (2–3 months old) weighing 25–30 g are used. Mice are injected with a sub–convulsive dose of PTZ (35 mg/kg, i.p.) (on Mondays, Wednesdays and Fridays) of each week for a total of 11 injections. After each PTZ injection, mice are observed for 30 min and the occurrence of convulsive activity is recorded. After 30 min, the mice are then injected with either Fasudil (25 mg/kg, i.p.) or Y–27632 (5 mg/kg, i.p.) and returned to their home cages until the next injection. Control mice for Fasudil andY–27632 receives saline.Rat[4]Male Wistar Kind A rats (200–250 g) are used. DMN (1 g/mL) is diluted ten times with saline (final concentration 1%) and 10 mg/kg per day of DMN is injected intraperitoneally (i.p.) on the first 3 days of each week for 4 weeks. Y27632 is given orally once per day at a dose of 30 mg/kg for 4 weeks starting on the day of the first injection of DMN. The dose of 30 mg/kg corrects hypertension in several rat models without toxicity. Twenty rats are randomized into four experimental groups (n=5 in each group) as follows: (1) S–S (injection of saline i.p. and oral administration of saline); (2) S–Y (injection of saline i.p. and oral administration of Y27632); (3) DMN–S (DMN i.p. and oral administration of saline); (4) DMN–Y (DMN i.p. and oral administration of Y27632). The rats are weighed every week. They are sacrificed at the end of the fourth week and the liver is excised. In addition, a blood sample is taken immediately before the rats are sacrificed.References:[1]. Ishizaki T, et al. Pharmacological properties of Y–27632, a specific inhibitor of rho–associated kinases. Mol Pharmacol. 2000 May;57(5):976–83.[2]. Xue ZW, et al. Rho–associated coiled kinase inhibitor Y–27632 promotes neuronal–like differentiation of adult human adipose tissue–derived stem cells.Chin Med J (Engl). 2012 Sep;125(18):3332–5.[3]. Inan S, et al. Antiepileptic effects of two Rho–kinase inhibitors, Y–27632 and fasudil, in mice. Br J Pharmacol. 2008 Sep;155(1):44–51.[4]. Tada S, et al. A selective ROCK inhibitor, Y27632, prevents dimethylnitrosamine–induced hepatic fibrosis in rats. J Hepatol. 2001 Apr;34(4):529–36.Caution: Product has not been fully validated for medical applications. For research use only.Tel: 609-228-6898 Fax: 609-228-5909 E-mail: tech@Address: 1 Deer Park Dr, Suite Q, Monmouth Junction, NJ 08852, USA。

抑制铁死亡可减轻脓毒症小鼠的心肌损伤:脂钙蛋白-2的作用

抑制铁死亡可减轻脓毒症小鼠的心肌损伤:脂钙蛋白-2的作用

脓毒症全球死亡率为10%,中国每年约有500万脓毒症患者,死亡率超过30%[1,2]。

心肌功能损伤是脓毒症住院患者中常见并发症[3],脓毒症引起的心功能不全是脓毒症死亡率高的主要原因[4],但其确切机制尚不完全清楚。

铁死亡最早被观察到铁离子螯合剂能抑制Erastin 诱导的癌细胞死亡不同于已知的细胞死亡途径,如凋亡、焦亡和坏死[5]。

铁死亡的机制主要涉及脂质过氧化物增加和脂质活性氧的进一步释放。

当谷胱甘肽过氧化物酶4(GPX4)或谷胱甘肽活性降低时,可诱导Inhibiting ferroptosis attenuates myocardial injury in septic mice:the role of lipocalin-2HUANG Yuhui 1,ZHANG Gongpeng 2,LIANG Huan 1,CAO Zhenzhen 3,YE Hongwei 1,GAO Qin 11Department of Physiology,2Department of Clinical Medicine,Bengbu Medical College,Bengbu 233000,China;3Department of Respiratory and Critical Care Medicine,First Affiliated Hospital of Bengbu Medical College,Bengbu 233000,China摘要:目的观察铁死亡在盲肠结扎与穿孔(CLP )法诱导的脓毒症小鼠心肌损伤中的作用,并探讨脂钙蛋白-2(Lcn2)在铁死亡中的可能作用。

方法选取8周龄雄性C57BL/6小鼠,采用CLP 法诱导脓毒症心肌损伤模型。

小鼠随机分为3组(10只/组):假手术组、脓毒症组(CLP ,小鼠接受CLP 手术)、脓毒症+铁死亡抑制剂Ferrostain-1组(CLP+Fer-1,小鼠腹腔注射浓度为5mg/mL 的Fer-15mg/kg ,1h 后接受CLP 手术)。

Promega-Maxwell

Promega-Maxwell

AS1490操作说明(本说明仅供参考,请以英文原版操作手册为准)用组织研磨机(机械珠磨破碎)进行预处理用户需提供的材料• 珠打装置(如MP Biomedicals FastPrep®-24仪器)• 无菌,防回吸移液枪头• 微型离心机或平板专用离心机1. 在每个离心管或孔的底部放置至多20mg的叶片组织。

2. 把一个珠子(或些许珠子,根据制造商的建议)放入每个管或孔。

3. 每个离心管或孔中加入300ul Tail Lysis Buffer (TLA)。

4. 每孔加入10ul RNase A(可选)。

注:如处理大量样品,使用前应立即准备足量的Tail Lysis Buffer和RNase A,将 310ul上述混合物加到每个样品中。

5. 使用制造商推荐的时间和速度运行珠打装置。

可能需要进行一些优化。

6. 将需要提取的离心离心管或板子放入离心机中,以最大速度旋转2分钟,以去除任何固体颗粒。

7. 制备Maxwell® RSC 样本制备托盘和试剂条(下一页制备样本托盘)。

8. 在每个Maxwell® RSC Plant DNA试剂条的第1孔中加入300ul无核酸酶水。

(1号孔是离试剂盒编码端最近、离用户最远的孔)。

9. 将每个植物裂解物样本从提取管或板子孔中转移到Maxwell® RSC试剂条的第1孔中。

只转移透明液体,注意不要转移任何固体物质到试剂条内。

用EP管、杵和液氮进行预处理用户需提供的材料• 颗粒杵(Sigma Aldrich Cat. # Z359947)• ClickFit EP管,1.5ml• 液氮• 无菌,防回吸移液枪头• 微型离心机1. 在1.5ml ClickFit EP管底部放置至多20mg的叶片组织。

2. 向植物组织样品中加入液氮。

让液体蒸发,冷冻样品。

3. 使用颗粒杵,尽可能彻底的对管壁研磨冷冻植物组织。

4. 每管加300 ul Tail Lysis Buffer (TLA)5. 每管加入10 ul RNase A(可选)。

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References: [1]. Wang, X., et al., The development of highly potent inhibitors for porcupine. J Med Chem, 2013. 56(6): p. 2700-4.
Caution: Not fully tested. For research purposes only Medchemexpress LLC

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P d t Data D t Sh t Product Sheet
Product Name: CAS No.: Cat. No.: MWt: Formula: Purity :
IWP L6 1427782-89-5;98%
Solubility:
DMSO
Mechanisms: Pathways:Wnt/Hedgehog/Notch; Target:Porcupine Biological Activity: IWP L6 is a Porcn inhibitor with EC50 of 0.5 nM. IC50 Value: 0.5 nM(EC50) [1] Target: Porcupine in vitro: IWP-L6 effectively suppressed the phosphorylation of dishevelled 2 (Dvl2) in HEK293 cells, a biochemical event associated with many Wnt-dependent cellular responses. IWP-L6 inhibits Wnt mediated branching morphogenesis in cultured embryonic kidneys [1]. in vivo: IWP-L6 is stable in human plasma over 24 h, it was rapidly metabolized in rat plasma (t1/2 = 190 min), murine plasma (t1/2 = 2 min), and the murine liver S9 fractions (t1/2 = 26 min). The major metabolites are the amide cleavage products. Similar species-dependent metabolitic profiles due to the in involvement ol ement of carbo carboxylesterase lesterase (CES) ha have e been reported with ith other dr drug g candidates candidates. Despite its modest metabolic stability in mouse-derived plasma, IWP-L6 was highly active in zebrafish. IWPL6 exhibited more pot...
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