Color correction for calorimetric color reproduction
Cyanacetylamino phenol color formers for color pho
专利名称:Cyanacetylamino phenol color formers for color photography发明人:KENDALL JOHN DAVID,FRY DOUGLASJAMES申请号:US59182845申请日:19450503公开号:US2441491A公开日:19480511专利内容由知识产权出版社提供摘要:Compounds of the general formula wherein the hydroxy group is ortho or para to the cyanacetylamino group and the position para to the hydroxy group is unsubstituted (including the corresponding naphthol and higher homologous compounds) are prepared by reacting together an aminophenol (or aminonaphthol or higher homologous compound) unsubstituted in the para position to the hydroxy group with ethyl cyanacetate. Cyanacet-o-hydroxyanilide is prepared by reacting o-aminophenol and ethyl cyanacetate in hot xylene in the presence of piperidine or other base. Cyanacet-o-hydroxy-p-toluidide is similarly prepared. Similar compounds are prepared from m-aminophenol, b -amino-a -naphthol, 2-amino-5-ethoxyphenol, 2-amino-3-methylphenol, and 2 - amino - 5 - chlorophenol. Specifications 503,825, 551,116, [both in Group XX], 551,117, 554,302 and 556,858, [Group XX], are referred to. The Provisional Specification refers also to the compounds 1-cyanacetylamino-2-hydroxy-4 - ethoxybenzene, 2 -cyanacetylamino - 1 - hydroxynaphthalene and 1-cyanacetylamino-2-hydroxy-6-methylbenzene.申请人:ILFORD LIMITED更多信息请下载全文后查看。
pr调色教学课件ppt
色彩偏差
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总结词:色彩偏差是指 图像的整体色调与真实 场景的色调存在差异, 通常是由于光线、设备 、环境等因素导致的。
详细描述
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1. 光线问题:在拍摄过 程中,如果光线不均匀 或者不稳定,就会导致 色彩偏差。解决方法是 使用专业的灯光设备, 确保光线充足且均匀。
2. 设备问题:不同的设 备对颜色的显示和还原 能力也有所差异,例如 不同的显示器或相机。 解决方法是校准设备, 确保颜色显示的一致性 。
3. 环境问题:环境因素 如温度、湿度等也会影 响颜色的呈现。解决方 法是尽量在恒温、恒湿 的环境中进行拍摄和后 期处理。
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pr调色实例教程
实例一:快速校正偏色视频
要点一
总结词
要点二
详细描述
通过使用PR中的色彩校正工具,可以快速有效地校正偏色 视频。
首先,打开需要校正的视频,选择颜色校正工具,然后根 据需要调整色调、饱和度等参数,最后应用调整。
详细描述
首先,选择需要应用灯光效果的视频片段, 然后选择合适的灯光类型和颜色,并调整灯 光的位置、大小等参数,最后应用调整。
实例四:制作黑白电影效果
总结词
通过使用PR中的颜色分级工具和黑白效果 预设,可以快速制作出黑白电影效果。
详细描述
首先,选择需要应用黑白电影效果的视频片 段,然后选择合适的黑白效果预设,并调整
色彩空间
不同的色彩空间对应不同 的色彩范围和精度。
视频色彩系统
RGB色彩模式
在视频中,大多数的图像格式采用RGB色彩模式。
CMYK色彩模式
主要用于印刷和打印。
HSL和HSV色彩模式
这两种模式将色彩分解为色调、饱和度和亮度。
SKIN COLOR MATCHING METHOD AND SYSTEM
专利名称:SKIN COLOR MATCHING METHOD AND SYSTEM发明人:Charles Conrad Carroll,Trevor Coward申请号:US11817536申请日:20060302公开号:US20090213379A1公开日:20090827专利内容由知识产权出版社提供摘要:A first method for matching skin colors comprises measuring a spectral reflectance curve of an area of skin and determining a blend of pigments to match the skin's color by combining spectral reflectance curves measured for pigments to produce a calculated spectral reflectance curve corresponding to that of the skin. The pigment blend is then used, for example, to pigment a medical prosthesis, so that it matches precisely its wearer's skin. This method avoids the problem of metamerism in which colors that match under a first illumination no longer match under a second illumination.A second method uses a database of pigment blends generated by the first method. The color of an area of skin is measured and a pigment blend is selected from the database that provides a closest match. Apparatus for carrying out such color matching is also provided, together with palettes of suitable pigments for skin color matching.申请人:Charles Conrad Carroll,Trevor Coward地址:London GB,London GB国籍:GB,GB更多信息请下载全文后查看。
Method and apparatus for correcting white balance,
专利名称:Method and apparatus for correcting whitebalance, method for correcting density andprogram recording medium发明人:Takahashi, Koji申请号:EP02025015.5申请日:20021108公开号:EP1311111A2公开日:20030514专利内容由知识产权出版社提供专利附图:摘要:The white balance correction method and apparatus estimate a colortemperature of a photographing light source with which a color image has been taken byusing at least gray and/or skin color information contained in an input color image and correct image signals of the color image based on the estimated color temperature. The density correction method multiplies image signals of respective pixels in an input color image by set coefficients to detect pixels having the multiplied image signals in the vicinity of a blackbody locus curve of skin color as skin color candidate pixels and assigns an average obtained for predetermined color signals from the skin color candidate pixels detected to a predetermined density of a color corresponding to the color signals on a print. The recording medium on which one or both of the white balance correction method and the density correction method are recorded in a computer-readable manner as a program to be executed by a computer.申请人:FUJI PHOTO FILM CO., LTD.地址:210 Nakanuma Minami-Ashigara-shi Kanagawa 250-01 JP国籍:JP代理机构:HOFFMANN - EITLE更多信息请下载全文后查看。
Calibration system and calibration method
专利名称:Calibration system and calibration method发明人:Satoru Ono申请号:US11498786申请日:20060804公开号:US20070030525A1公开日:20070208专利内容由知识产权出版社提供专利附图:摘要:A calibration system includes: an image formation unit that outputs a patch sheet having a plurality of patch patterns based on different gradation values for each color of color material that is used; a selection unit that receives an instruction to select a device from among a plurality of color measurement devices comprising a first colormeasurement device and a second color measurement device of a lower color measurement accuracy than that of the first color measurement device; a color measurement unit that receives data of a color measurement result for the patch sheet that is color-measured by the color measurement device selected by the selection instruction; a color measurement value determination unit that acquires color measurement values for each of the patch patterns on the basis of the color measurement result data received; and a color adjustment unit that generates color correction information of the image formation unit on the basis of the color measurement values acquired. In the system, the output patch sheet is common irrespective of the color measurement device selected by the selection instruction and, when the second color measurement device is selected by the selection instruction, the color measurement value determination unit acquires, by averaging a plurality of the color measurement result data that exist in the patch pattern, the color measurement values for each of the patch patterns.申请人:Satoru Ono地址:Nagano-ken JP国籍:JP更多信息请下载全文后查看。
ZENO(R)TRANSLUCENT氧化锆基底瓷颜色差异对修复体颜色的影响
ZENO(R)TRANSLUCENT氧化锆基底瓷颜色差异对修复体颜色的影响史也;张新媛;吴效民【摘要】目的:通过体外实验,研究氧化锆基底瓷颜色的差异对氧化锆全瓷修复体颜色的影响.方法:选取4种不同颜色的氧化锆瓷块制备边长为10mm,厚度为0.5mm 的正方形试件各10件,依次分为A、B、C、D四组,并均匀涂塑B3色的牙本质瓷1.0mm,用Crystaleye比色仪依次测量全部试件的色度值,每个试件测三次,取平均值,并计算组间△E值.再选用Crystaleye分析软件中的Vita Classical比色板对试件进行比色,记录比色结果及各试件与该比色片的色差值.每个试件测三次,取平均值作为最终结果.用SPSS 12.0软件对不同颜色的氧化锆基底瓷与试件颜色的关系进行相关性和回归性分析,并对各组的色度学参数进行方差分析和独立样本t检验.结果:ABCD四组试件组间的Lab值差异均具有统计学意义.四组试件组间的色差值均大干1.5.应用Crystaleye分析软件中的Vita Classical比色板的比色结果均为B3.其中与B3比色片色差值最小的为C组试件.结论:氧化锆基底瓷的颜色对修复体颜色有影响,临床中应根据配色指导选择合适基底瓷颜色.【期刊名称】《口腔颌面修复学杂志》【年(卷),期】2015(016)005【总页数】4页(P310-313)【关键词】氧化锆;基底瓷;颜色【作者】史也;张新媛;吴效民【作者单位】北京协和医院口腔科北京100730;北京协和医院口腔科北京100730;北京协和医院口腔科北京100730【正文语种】中文【中图分类】R783.1近年来,全瓷修复体凭借其优良的机械性能及美学效果越来越受到患者和医师的青睐[1]。
其中氧化锆材料因其良好的的机械性能而被广泛应用于口腔临床中,但这种材料的临床美学效果却受多因素影响,且研究尚不完全[2-5]。
临床常用的氧化锆全瓷修复体通常分为基底瓷和饰瓷两层。
色彩魅力
Natural Hair天然发质
Pigment Type Melanocyte cells create pigment granules • Found within the root bulb • Triggered by a chemical catalyst
Байду номын сангаас
色素产生区域
角蛋白 区域 黑素细胞
什么是色轮? 展示三原色和三付色 颜色是减去性的 对冲色能够互相抵消 棕色象征性的位于中央
Fit for Colour DAY 1 1 Theory 2 General Products 3 IGORA System
The Colour Wheel色轮
Complementary Colours Yellow - Violet Orange - Blue Red – Green
skp_colour_wheel.swf
Natural Hair天然发质
Colour in Hair Comes from pigment granules Type & quantity determines tone & depth Pigment size & shape varies Cuticle condition influences perception
1 理论 染发原理 认识色轮 了解天然发质 2 普通产品
Fit for Colour DAY 1 1 Theory 2 General Products 3 IGORA System
染发产品分类 氧化色素和直接色素 暂时性/半永久/永久性产品
课程大纲
3 IGORA System Features & Benefits Numbering System IGORA Product Range
如何回复编辑和审稿人
如何回复编辑和审稿人 Company Document number:WTUT-WT88Y-W8BBGB-BWYTT-19998专题半月谈 - 如何回复编辑和审稿人作者:QIN 提交日期:2010-12-09 06:25:55 PM | 访问量:713专题半月谈 - 如何回复编辑和审稿人应战友要求,在版主的支持下,本人在此设立一个半月谈专题-如何回复编辑和审稿人实例分析。
需说明以下几点:1.为了突出问题的典型性,有些“实例“已经过修饰,故已不是作者原稿(或原意)。
2.修改和注解谨代表本人观点。
对于同一问题可能有不同或更好的修改方法或回答方式。
欢迎讨论。
3.同时亦欢迎各高级战友按以下格式增加实例(请注意版权问题)。
希望战友回帖时紧紧围绕主题,不要讨(争)论与主题无关的话题。
论文题目:所投杂志:投稿结果:编辑/审稿人问题:作者原答:建议改答:注解请写在文中相应的地方或单独列出。
15 January 2009 (第一讲)给编辑的回复信论文题目:Pharmacokinetic and pharmacodynamic studies on the antivirus effect s of A (一种中草药) against virus B (一种病毒)所投杂志:Life Sciences投稿结果:这次大修后又经过一次小修,被接受发表编辑信内容(注:有删节):Dear Mr. XXX,Your manuscript has been examined by the editors and qualified refere e. We think the manuscript has merit but requires revision before wecan accept it for publication in the Journal. Careful consideration m ust be given to the points raised in the reviewer comments, which are enclosed below.If you choose to submit a revision of your manuscript, please incorpo rate responses to the reviewer comments into the revised paper. A com plete rebuttal with no manuscript alterations is usually considered i nadequate and may result in lengthy re-review procedures.A letter detailing your revisions point-by-point must accompany the r esubmission.You will be requested to upload this Response to Reviewers as a separ ate file in the Attach Files area.We ask that you resubmit your manuscript within 45 days. After this t ime, your file will be placed on inactive status and a further submis sion will be considered a new manuscript.You will see a menu item called Submission Needing Revision. You will find your submission record there.Yours sincerely,Joseph J. Bahl, PhDEditorLife SciencesFormat Suggestion: Please access the Guide to Authors at our website to check the format of your article. Pay particular attention to our References style.Reviewers' comments:Reviewer #1:XXXXX (略)Reviewer #2:XXXXX (略)Editors note and suggestions:(注:编辑的建议)Title: Re-write the title to read more smoothly in contemporary Engli sh>>>Pharmacokinetic and pharmacodynamic studies of the antiviral effects of A against virus B.Abstract: Re-write the abstract to read more smoothly.A, an alkaloid isolated from C (注:一种中草药), was tested for antiv iral activity against virus B. Both in vitro and in vivo assays along with serum pharmacological experiments showed A to have potent antiv iral activity. The pharmacokinetic profile of A in Sprague/Dawley rat plasma after oral administration was measured by HPLC. Blood samples taken at selected time points were analyzed to study potential chang es in antiviral pharmacodynamics as measured by infectivity of viruse s. From the similarity of the serum concentration profiles and antivi ral activity profiles it is concluded that A it self, rather than a m etabolite, exerted the effect against the virus prior to bioinactivat ion. The need for effective clinical agents against virus B and theseresults suggest the possibility of benefit from further experiments with A.The authors should check to be sure that the terms blood samples, pla sma and serum are always used appropriately throughout the abstract a nd text.Introduction: some sentences can be made less passive. example 1st pa ragraph>>>> A appears to be the most important alkaloid isolated from the pl ant, its structural formula is shown in Fig 1. ... While it produced a general inhibition of antibody production lymphocyte proliferation was stimulated (Xia and Wang, 1997). These pharmacological properties suggest a potential use in the treatment of viral myocarditis agains t virus B that could be studied in experiments in cell culture and an imals.>>>The authors should check the entire manuscript for spelling errors (example given: in your text alkaloid is incorrectly spelled alkaloi d)>>>The authors should read the guidelines to the authors and not incl ude the first name of the authors being cited in the text. In the ref erence section the first name should be abbreviated as shown in the g uideline to authors (thus the earlier text reference should be (Liu e t al., 2003)and the remaining one should be (Chen et al., 2002)>>>>>The authors instead of directly answering the first complex ques tion of reviewer #1 may include the three questions as future researc h aim in the discussion section.>>>>>>Rather than redrawing figure the authors may choose to amend th e wording of the statistical analysis section to state that the resul t of tables are means +-SEM and for figures are +- SD.>>>>> reviewer #1 comment number 8 and reviewer # 2 comment 3 might b e satisfied by inclusion of a representative photo of cells and heart showing CPE. Remember most readers of the journal have never seen wh at you are trying to describe.Joseph Bahl, PhD Editor 2 Life Sciences作者回复信原稿:Dear Dr. Bahl,I’m (注:正式信函不要简写)very appreciate (注:不适合作为给编辑回信的开始,同时有语法错误)for your comments and suggestions.I (注:实际上是学生做的)have conducted in vivo antivirus experiment s again (注:要表明是应审稿人或编辑建议而作). Mice were sacrificed on 15 days and 30 days after infection. Death rate, heart weight to b ody weight ratio (HW/BW), virus titers and pathologic slices (注:用词错误)were calculated(注:用词不当). Production of mRNA of IL-10, IFN-γand TNF-αwere (注:语法错误)measured by RT-PCR.I have revised this manuscript and especially paid much attention to your comments and suggestions. I would like to re-submit it to LIFE S CIENCE. Title of manuscript has been changed to “The antivirus effects of A against virus B and its pharmacokinetic behaviour in SD rats serum” to make it more clear and smooth.Answers to Reviewers’ questions were as follows: (注:可附在给编辑的回复信后)Reviewer #1:XXXXXReviewer #2:XXXXXEditors note and suggestions:Title: Re-write the title to read more smoothly in contemporary Engli shAnswer:I have rewrite the title to “The antivirus effects of A against viru s B and its pharmacokinetic behaviour in SD rats serum” to make it m ore clear and smooth(注:多处语法错误).Abstract: Re-write the abstract to read more smoothly.Answer:I have revise the abstract carefully to make it more smooth and infor mative(注:语法错误).The authors should check to be sure that the terms blood samples, pla sma and serum are always used appropriately throughout the abstract a nd text.Answer:I have paid attention to this question and it is clearer (注:不具体).Introduction:some sentences can be made less passive.Answer:I have revise the whole paper to make sentences less passive and obta ined help of my colleague proficient in English (注:语法错误,句子不通顺).The authors should check the entire manuscript for spelling errors Answer:I’m very sorry to give you so much trouble for those spelling errors (注:不必道歉,按建议修改即可). I have carefully corrected them.The authors should read the guidelines to the authors and not include the first name of the authors being cited in the text. In the refere nce section the first name should be abbreviated as shown in the guid eline to authors (thus the earlier text reference should be (Liu et a l., 2003) and the remaining one should be (Chen et al., 2002)Answer:I changed the style of references.Rather than redrawing figure the authors may choose to amend the word ing of the statistical analysis section to state that the result of t ables are means +-SEM and for figures are +- SD.Answer:(注:作者请编辑公司帮回答)reviewer #1 comment number 8 and reviewer # 2 comment 3 might be sati sfied by inclusion of a representative photo of cells and heart showi ng CPE. Remember: most readers of the journal have never seen what yo u are trying to describe.Answer:Thank you for your suggestions. I have supplemented pictures of cardi ac pathologic slices in the paper (Fig2).I have to apologize for giving you so much trouble because of those m isspelling and confusing statements (注:一般不是延误或人为失误,不必轻易道歉,按建议修改即可). Your comments and suggestions really he lped me a lot. I have put great efforts to this review. I wish it can be satisfactory.If there’s (注:正式信函不要简写)any information I can provide, pl ease don’t hesitate to contact me.Thank you again for your time and patience. Look forward to hear (注:语法错误)from you.Yours SincerelyXxxx Xxxx (通讯作者名)建议修改稿:Dear Dr. Bahl,Thanks you very much for your comments and suggestions.As suggested, we have conducted in vivo antivirus experiments. Mice w ere sacrificed on 15 days and 30 days after infection with virus B. M ortality, heart weight to body weight ratio (HW/BW), virus titers and pathologic scores were determined. In addition, mRNA expression of I L-10, IFN-γ and TNF-α were measured by RT-PCR.We have revised the manuscript, according to the comments and suggest ions of reviewers and editor, and responded, point by point to, the c omments as listed below. Since the paper has been revised significant ly throughout the text, we feel it is better not to highlight the ame ndments in the revised manuscript (正常情况最好表明修改处).The revised manuscript has been edited and proofread by a medical edi ting company in Hong Kong.I would like to re-submit this revised manuscript to Life Sciences, a nd hope it is acceptable for publication in the journal.Looking forward to hearing from you soon.With kindest regards,Yours SincerelyXxxx Xxxx (通讯作者名)Replies to Reviewers and EditorFirst of all, we thank both reviewers and editor for their positive a nd constructive comments and suggestions.Replies to Reviewer #1:Xxxxx (略)Replies to Reviewer #2:Xxxxx (略)Replies to the Editors note and suggestions:Title: Re-write the title to read more smoothly in contmeporary Engli shAnswer:I have rewrite the title to “The antivirus effects of Sophoridine ag ainst Coxsackievirus B3 and its pharmacokine tics in rats” to make it more clear and read more smoothly.Abstract: Re-write the abstract to read more smoothly.Answer:I have rewritten the abstract to make it more informative and read mo re smoothly.The authors should check to be sure that the terms blood samples, pla sma and serum are always used appropriately throughout the abstract a nd text.Answer:I have paid attention to this issue, and they are now used appropriat ely throughout the abstract and text in the revised manuscript.Introduction:some sentences can be made less passive.Answer:I have revised the whole paper to make sentences less passive with th e help of the editing company.The authors should check the entire manuscript for spelling errorsAnswer:This has been done by us as well as the editing company.The authors should read the guidelines to the authors and not include the first name of the authors being cited in the text. In the refere nce section the first name should be abbreviated as shown in the guid eline to authors (thus the earlier text reference should be (Liu et a l., 2003) and the remaining one should be (Chen et al., 2002)Answer:I have changed the style of references according to the journal.Rather than redrawing figure the authors may choose to ament the word ing of the statistical analysis section to state that the result of t ables aremeans +-SEM and for figures are +- SD.Answer:SD has been used throughout the text, and shown in the Figs. 3 and 4 in the revised manuscript.reviewer #1 comment number 8 and reviewer # 2 comment 3 might be sati fied by inclusion of a representative photo of cells and heart showin g CPE. Remember: most readers of the journal have never seen what you are trying to describe.Answer:Thank you very much for the suggestion. I have added pictures of card iac pathologic changes in the revised manuscript (Fig. 2).很好的经验,谢谢分享!31 January 2009 (第二讲)给审稿人的回复信论文题目:Clinical implications of XXXX (一种病理指标) in X cancer所投杂志:BMC Cancer.结果:这次大修后被接受发表(同时编辑在接受信中提出课题是否得到伦理委员会同意的问题。
Differentiation of Human Pluripotent Stem Cells into Retinal Cells
87M.A. Hayat (ed.), Stem Cells and Cancer Stem Cells, Volume 6,DOI 10.1007/978-94-007-2993-3_9, © Springer Science+Business Media B.V . 20129A bstractRetinal and macular degeneration disorders are characterized by a progressive loss of photoreceptors, which causes visual impairment and blindness. In some cases, the visual loss is caused by dysfunction, degen-eration and loss of underlying retinal pigment epithelial (RPE) cells and the subsequent death of photoreceptors. The grim reality is that there is no successful treatment for most of these blindness disorders. Cell therapy aimed at replenishing the degenerating cells is considered a potential ther-apeutic approach that may delay, halt or perhaps even reverse degenera-tion, as well as improve retinal function and prevent blindness in the aforementioned conditions. Human embryonic stem cells (hESC) and induced pluripotent stem cells (iPSCs) may serve as an unlimited donor source of photoreceptors and RPE cells for transplantation into degenerat-ing retinas and for retinal disease modeling.I ntroductionThe vertebrate eyes form as bilateral evaginations of the forebrain, called optic vesicles (Martínez-Morales et al. 2004 ; Fig. 9.1a ). During develop-ment, the optic vesicles begin to invaginate to form a cup-shaped structure, the optic cup. The inner, thicker neural layer of the optic cup differ-entiates into the neural retina, and the outer, thin-ner pigmented layer forms the retinal pigmentepithelium (RPE). At the early developmental stages, the neuroepithelial cells that compose the optic vesicle are morphologically and molecu-larly identical and are all able to give rise to neu-ral retina and RPE. Exogenous signals coming from the adjacent tissues, including factors from the fi broblast growth factor (FGF) and transform-ing growth factor beta (TGF b ) families, dictate the fate of these cells. The mature vertebrate ret-ina is comprised of six types of neurons and one type of glia (the Müller glia). These seven cell types constitute three nuclear layers: retinal gan-glion cells in the ganglion cell layer (GCL); the horizontal, bipolar and amacrine interneurons, and Müller glial cells in the inner nuclear layer (INL); and rod and cone photoreceptors in the outer nuclear layer (ONL; Harada et al. 2007;M . I delson • B . R eubinoff (*)T he Hadassah Human Embryonic Stem Cell Research Center, The Goldyne Savad Institute of Gene Therapy & The Department of Obstetrics and Gynecology , H adassah University Medical Center ,E in Kerem 12000 ,J erusalem 91120 ,I srael e -mail: b enjaminr@ekmd.huji.ac.il D ifferentiation of HumanPluripotent Stem Cells into Retinal Cells Masha Idelson and Benjamin Reubinoff88M. Idelson and B. ReubinoffFig. 9.1b ). The photoreceptor cells capture lightphotons and transform their energy into electrical signals by a mechanism called phototransduction. The visual pigment which is utilized in this process is located on membranal discs in the outer seg-ments of photoreceptors. The outer segments are continuously renewed: the old discs are shed and new disks form. When the photoreceptors absorb light, they send the signal through the retinal interneurons to the ganglion cells which transmit the electrical impulse to the brain by their axons forming the optic nerve. Rods are responsible for night vision, whereas cones are responsible for color vision and detecting fi ne details. The macula is a small part of the retina which is rich in cones and responsible for detailed central vision.R PE cells that compose the outer layer of the optic cup are pigmented cuboidal cells which lie between the neural retina and the choriocapil-laris, which include the blood vessels supplying the retina. The multiple villi on their apical side are in direct contact with the outer segments ofextraocular mesenchymeabneural retinalensoptic nerveoptic cupsurface ectodermRPEFGFoptic vesiclechoroidBM RPE cone ONLINL GCLlightHC BC MC ACONrod F ig. 9.1 D evelopment and structural arrangement of the retina. ( a ) Schematic representation of retinal development including the transition from optic vesicle to optic cup and retinal patterning. ( b ) Schematic diagram of retinal cells arrangement and connections. A bbreviations :A C amacrinecell, B C bipolar cell, B M Bruch’s membrane, G CL gan-glion cell layer, H C horizontal cell, I NL inner nuclear layer, M C Müller cell, O N optic nerve, O NL outer nuclear layer89 9 Differentiation of Human Pluripotent Stem Cells into Retinal Cellsthe photoreceptor cells; on their basal side, the RPE is in contact with the underlying basal mem-brane, termed Bruch’s membrane that separates the RPE from the choroid. These cells play cru-cial roles in the maintenance and function of the retina and its photoreceptors. As a layer of pig-mented cells, the RPE absorbs the stray light that was not absorbed by the photoreceptors. The RPE cells form a blood–retinal barrier due to decreased permeability of their junctions. The RPE cells transport ions, water, and metabolic end products from the retina to the bloodstream. They are involved in supplying the neural retina with nutrients from the bloodstream, such as glu-cose, retinol, and fatty acids. Another important function of the RPE is the phagocytosis of shed photoreceptor outer segments. After the outer segments are digested, essential substances such as retinal are recycled. Retinal is also recycled and returned to photoreceptors by the process known as the visual cycle. The precise functioning of the RPE is essential for visual performance. Failure of one of these functions can lead to degeneration of the retinal photoreceptors, vision impairment and blindness.T here are many inherited and age-related eye disorders that cause degeneration of the retina as a consequence of loss of photoreceptor cells. Retinal and macular degeneration disorders can be divided into two main groups. The fi rst group primarily affects the photoreceptors and involves the majority of cases of retinitis pigmentosa. In the second group, the primary damage is to the adjacent RPE cells, and as a consequence of this damage, the photoreceptors degenerate. This group includes age-related macular degeneration, Stargardt’s macular dystrophy, a subtype of Leber’s congenital amaurosis in which RPE65 is mutated, Best’s disease and some cases of retini-tis pigmentosa, as well.W ith regard to retinitis pigmentosa (RP), it is a group of inherited retinal degeneration diseases that are caused, as mentioned above, by a primary progressive loss of rod and cone photoreceptors, followed by a subsequent degeneration of RPE (Hartong et al. 2006). The disease affects approxi-mately 1.5 million patients worldwide and is the most common cause of blindness in people under 70 years of age in the western world. The disease can be characterized by retinal pigment deposits visible on the fundus examination. In most cases, the disease primarily affects rods. At later stages of the disease, the degeneration of cones takes place. As a consequence of disease progression, the patients’ night vision is reduced. Patients initially lose peripheral vision while retaining central vision (a visual status termed “tunnel vision”). In advanced cases, central vision is also lost, commonly at about 60 years of age. The disease affects about 1 in 4,000. The inheritance can be autosomal-recessive, autosomal-dominant or X-linked (in ~50–60%, 30–40%, and 5–15% of cases, respectively). Mutations in more than 140 genes have been iden-tifi ed as causing RP (Hartong et al. 2006).Among these genes are those involved in phototransduc-tion, like rhodopsin, the a- and b- subunits of phos-phodiesterase, the a- and b- subunits of Rod cGMP gated channel and arrestin. The additional muta-tions were found in genes encoding structural pro-teins, like peripherin, rod outer segment protein and fascin. They were also found in transcription factors involved in photoreceptors’ development such as Crx and Nrl, and in other genes, whose products are involved in signaling, cell-cell interac-tion and trafficking of intracellular proteins. Currently, there is no effective cure for RP. Treatment with vitamin A palmitate, omega-3 fatty acids and other nutrients may somewhat slow the rate of the disease progression in many cases. Reduction in exposure to light was also shown to decrease the rate of retinal degeneration.A mong the group of retinal degenerations that are caused by primary loss of RPE cells or their function, age-related macular degeneration (AMD) is the most frequent condition and the leading cause of visual disability in the western world (Cook et al. 2008).Among people over 75 years of age, 25–30% are affected by AMD, with progressive central visual loss that leads to blindness in 6–8%. The retinal degeneration pri-marily involves the macula. The dry form of AMD is initiated by hyperplasia of the RPE and formation of drusen deposits, consisting of meta-bolic end products underneath the RPE or within the Bruch’s membrane. It may gradually progress into the advanced stage of geographic atrophy90M. Idelson and B. Reubinoff with degeneration of RPE and photoreceptorsover large areas of the macula causing central visual loss. Ten percent of dry AMD patients will progress to neovascular (wet) AMD, with blood vessels sprouting through the Bruch’s membrane with subsequent intraocular leakage and/or bleed-ing, accelerating the loss of central vision. While the complicating neovascularization can be treated with anti-VEGF agents, currently there is no effective treatment to halt RPE and photore-ceptor degeneration and the grim reality is that many patients eventually lose their sight (Cook et al. 2008).S targardt’s macular dystrophy (SMD) is the most common form of inherited macular dystro-phy affecting children (Walia and Fishman 2009). The disease is symptomatically similar to AMD. The prevalence of SMD is about 1 in 10,000 chil-dren. The disease involves progressive central visual loss and atrophy of the RPE beneath the macula following accumulation of lipofuscin in RPE cells, which is suggested to consist of non-degradable material, derived from ingested pho-toreceptor outer segments. The inheritance is predominantly autosomal recessive, although an autosomal dominant form has also been described. The mutation in the ABCA4 gene was found to be a most common cause of SMD. The product of the ABCA4 gene is involved in energy transport to and from photoreceptors. The mutated protein cannot perform its transport function and, as a result, photoreceptor cells degenerate and vision is impaired. Currently, there is no effective treat-ment for SMD.C ell therapy to replenish the degenerating cells appears as a promising therapeutic modality that may potentially halt disease progression in the various retinal and macular degeneration dis-orders caused by loss and dysfunction of RPE cells and photoreceptors (da Cruz et al. 2007).I n this chapter we will discuss the potential of human pluripotent cells which includes human embryonic stem cells (hESC) and induced pluripotent stem cells (iPSCs), to gen-erate various types of retinal cells that could be used for transplantation therapy of retinal degen-eration disorders and disease modeling for drug discovery. C ell Therapy of Retinal and Macular DegenerationsT he eye is an attractive organ for cell therapy as it is easily accessible for transplantation and for simple monitoring of graft survival and potential complications by direct fundoscopic visualiza-tion. Anatomically, it is a relatively confi ned organ limiting the potential of unwanted extra-ocular ectopic cell distribution, and a low number of cells are required to replenish the damaged cells. The eye is also one of the immune privi-leged sites of the body.T he concept of replacing dysfunctional or degenerated retina by transplantation has been developing ever since the fi rst retina-to-retina transplant in 1986 (Turner and Blair 1986).In most studies, primary retinal immature (fetal) tissue has been used as donor material. It was demonstrated that such transplants can survive, differentiate, and even establish connections with the host retina to a limited degree (Ghosh et al. 1999). The subretinal transplantation of healthy RPE has some advantages over neural retinal transplantation, as it concerns only one cell type that is not involved in neural networking. Transplantation of RPE has been studied exten-sively in animal models (Lund et al. 2001).The most commonly used animal model of retinal degeneration is the Royal College of Surgeons (RCS) rat model, in which primary dysfunction of the RPE occurs as a result of a mutation in the receptor tyrosine kinase gene M ertk(D’Cruz et al. 2000). This leads to impaired phagocytosis of shed photoreceptor outer segments, with sec-ondary degeneration and progressive loss of pho-toreceptors within the fi rst months of life. It was reported that rat and human RPE cells rescued photoreceptor cells from degeneration when transplanted into the subretinal space of RCS rats (Li and Turner 1988; Coffey et al. 2002).The ability of transplanted RPE cells to restore retinal structure and function has been demonstrated in clinical trials. In humans, autologous transplanta-tions of peripheral RPE as well as macular trans-locations onto more peripheral RPE provide a proof that positioning the macula above relatively91 9 Differentiation of Human Pluripotent Stem Cells into Retinal Cellshealthier RPE cells can improve visual functionin AMD patients (Binder et al. 2004; da Cruz et al. 2007). Nevertheless, the surgical procedures for autologous grafting are challenging and are often accompanied by signifi cant complications. In addition, autologous RPE transplants may carry the same genetic background, environmen-tal toxic and aging-related effects that may have led to macular RPE failure and the development of AMD in the patient. It is also problematic to use autologous cells when all the RPE cells are damaged. Cell sources that can be used for such therapy include allogeneic fetal and adult RPE (Weisz et al. 1999; Binder et al. 2004; da Cruz et al. 2007). However, the use of fetal or adult retinal tissues for transplantation is severely lim-ited by ethical considerations and practical prob-lems in obtaining sufficient tissue supply. The search for a cell source to replace autologous RPE such as immortalized cell lines, umbilical cord-derived cells as well as bone marrow-derived stem cells continues.T he derivation of hESCs more than a decade ago has raised immense interest in the potential clinical use of the cells for regeneration (Thomson et al. 1998; Reubinoff et al. 2000).Along the years, signifi cant progress has been made towards the use of hESCs in clinical trials.T he other promising source of cells for transplantation therapy is iPSCs that are simi-lar to hESCs in their stemness characteristics and pluripotency. These cells could be gener-ated from different human somatic cells by transduction of four defi ned transcription fac-tors: Oct3/4, Sox2, Klf4, and c-Myc (Takahashi et al. 2007).G eneration of RPE and neural retina from hESCs and iPSC has numerous advantages, as it can be done from pathogen-free cell lines under good manufacturing practice (GMP) conditions with minimal variation among batches. Such cells can be characterized extensively prior to preclinical studies or for clinical applications, and an unlimited numbers of donor cells can be generated from them. In the following para-graphs, strategies for induction of differentiation of hESCs and iPSCs towards RPE and neural retina fate are reviewed. D ifferentiation into Retinal Pigment EpitheliumI t was reported for the fi rst time in mice and pri-mates that the differentiation of ES cells into RPE could be induced by co-culture with PA6 stromal cells (Kawasaki et al. 2002; Haruta et al. 2004). The resulting cells had polygonal epithelial mor-phology and extensive pigmentation. The cells expressed the markers that are characteristic of RPE. They developed typical ultrastructures and exhibited some functions of RPE. The differenti-ation of hESC into RPE was first reported by Klimanskaya et al. (2004).According to their protocol, hESCs underwent spontaneous differ-entiation by overgrowth on mouse embryonic fibroblasts (MEF), in feeder-free conditions or, alternatively, as embryoid bodies (EBs) in com-bination with withdrawal of bFGF from the medium. The yield of the formation of RPE cells after 4–8 weeks of spontaneous differentiation was relatively low; for example,<1% of EBs con-tained pigmented cells at this stage. However, after 6–9 months in culture, all the EBs contained pigmented cells. The areas of pigmented cells could be further isolated mechanically and prop-agated by passaging as RPE lines. Klimanskaya and colleges characterized the hESC-derived RPE cells by transcriptomics and demonstrated their higher similarity to primary RPE tissue than to human RPE lines D407 and ARPE-19. The low yield of spontaneously differentiating RPE cells was improved by induction of differentia-tion with Wnt and Nodal antagonists, Dkk1 and LeftyA, respectively, the factors that are sug-gested to promote retinal differentiation. This treatment gave rise to pigmented cells within 38% of the hESC colonies after 8 weeks (Osakada et al. 2008). Immunostaining with the ZO-1 anti-body showed that by day 120, hESC-derived pig-mented cells formed tight junctions (about 35% of total cells). We showed that differentiation toward the neural and further toward the RPE fate could be augmented by vitamin B3 (nicotin-amide; Idelson et al. 2009).We further showed that Activin A, in the presence of nicotinamide, effi ciently induces and augments differentiation92M. Idelson and B. Reubinoffinto RPE cells. This is in line with the presumed role of Activin A in RPE development i n vivo .In the embryo, extraocular mesenchyme-secreted members of the TGF b superfamily are thought to direct the differentiation of the optic vesicle into RPE (Fuhrmann et al. 2000).Under our culture conditions, when the cells were grown in suspen-sion as free-fl oating clusters, within 4 weeks of differentiation, 51% of the clusters contained pigmented areas and about 10% of the cells within the clusters were pigmented. When we modifi ed the differentiation conditions to includea stage of monolayer culture growth, the yield of the RPE-like pigmented cells was signifi cantly improved and 33% of the cells were pigmented after 6 weeks of differentiation. The derivation of RPE from hESCs and iPSCs without any external factor supplementation was also demonstrated by other groups (Vugler et al. 2008 ; Meyer et al. 2009 ; Buchholz et al. 2009).T he hESC-derived RPE cells were extensively characterized, including demonstration, both at the mRNA and the protein levels, of the expres-sion of RPE-specifi c markers, such as RPE65, CRALBP, Bestrophin, Tyrosinase, PEDF, PMEL17, LRAT, isoforms of MiTF abundant in RPE, and others. The cells expressed markers of tight junctions that join the adjacent RPE cells: ZO-1, occludin and claudin-1 (Vugler et al. 2008 ) . Electron microscopic analysis revealed that the hESC-derived RPE cells showed features characteristic of RPE. The cells were highly polarized with the nuclei located more basally, and the cytoplasm with the mitochondria and melanin granules of different maturity more api-cally. A formation of basal membrane was observed on the basal surface of the RPE cell. Similar to putative RPE, the hESC-derived RPE basal membrane was shown to be composed of extracellular matrix proteins, collagen IV , lami-nin and fi bronectin (Vugler et al.2008).The appearance of apical microvilli was demonstrated at the apical surface of the RPE. The presence of tight and gap junctions on the apical borders of the RPE cells was also confi rmed by electron microscopy. O ne of the most important functions of RPE cells i n vivo is phagocytosis of shed photoreceptor outer segments, as part of the continuous renewal process of rods and cones. The hESC-derived RPE cells demonstrated the ability to phagocyto-size latex beads or purifi ed photoreceptor outer segments, confi rming that these cells are func-tionali n vitro . It may be concluded from all these studies that human pluripotent stem cells have a potential to give rise to pigmented cells exhibiting the morphology, marker expression and functionof authentic RPE.D ifferentiation into Retinal Progenitors and Photoreceptors O ur group showed, for the fi rst time, the potential of highly enriched cultures of hESC-derived neu-ral precursors (NPs) to differentiate towards the neural retina fate (Banin et al. 2006).We demon-strated that the NPs expressed transcripts of key regulatory genes of anterior brain and retinal development. After spontaneous differentiation i n vitro , the NPs gave rise to progeny expressing markers of retinal progenitors and photoreceptor development, though this was uncommon and cells expressing markers of mature photorecep-tors were not observed. We showed that after transplantation into rat eyes, differentiation into cells expressing specifi c markers of mature photoreceptors occurred only after subretinal transplantation (between the host RPE and pho-toreceptor layer) suggesting that this specifi c microenvironment provided signals, yet unde-fi ned, that were required to support differentia-tion into the photoreceptoral lineage.P rogress towards controlling and inducing the differentiation of hESCs into retinal progenitors and neurons i n vitro was reported in the study of Lamba et al. ( 2006).They treated hESC-derived EBs for 3 days with a combination of factors,including Noggin, an inhibitor of BMP signaling, Dkk1, a secreted antagonist of the Wnt signaling pathway and insulin-like growth factor 1 (IGF-1), which is known to promote retinal progenitor dif-ferentiation. The cultivation of EBs with these factors was followed by differentiation on Matrigel or laminin for an additional 3 weeks in the presence of the combination of the three93 9 Differentiation of Human Pluripotent Stem Cells into Retinal Cellsfactors together with bFGF. Under these culture conditions, the majority of the cells developed the characteristics of retinal progenitors and expressed the specifi c markers Pax6 and Chx10 (82% and 86% of the cells, respectively). The authors showed that after further differentiation, the cells expressed markers of photoreceptor development Crx and Nrl (12% and 5.75%, respectively). About 12% of the cells expressed also HuC/D, the marker of amacrine and ganglion cells. The expression of markers of the other sub-types of retinal neurons was demonstrated, as well. However, only very few cells (<0.01%) expressed markers of mature photoreceptors, blue opsin and rhodopsin. The abundance of cells expressing markers of photoreceptors could be accelerated by co-culture with retinal explants, especially when the explants originated from mice bearing a mutation that causes retinal degeneration.T o better characterize the phenotype of retinal cells obtained with this differentiation protocol, a microarray-based analysis comparing human retina to the hESC-derived retinal cells was per-formed (Lamba and Reh 2011).It was demon-strated that gene expression in hESC-derived retinal cells was highly correlated to that in the human fetal retina. In addition, 1% of the genes that were highly expressed in the hESC-derived cultures could be attributed to RPE and ciliary epithelium differentiation.A n alternative protocol for the derivation of retinal progenitors and photoreceptors was pro-posed by Osakada et al. (2008).Similar to the protocol for the derivation of RPE cells, they used serum-free fl oating cultures in combination with the Dkk1 and LeftyA. After 20 days of cul-ture in suspension, the cells were replated on poly-D-lysine/laminin/fi bronectin-coated slides. Osakada and co-authors demonstrated that on day 35 in culture, about 16% of colonies were positive for retinal progenitor markers Rx and Pax6. Differentiation towards photoreceptor fate was augmented in the presence of N2 by treat-ment with retinoic acid and taurine, which are known inducers of rod fate differentiation. Under these conditions, after an extended culture period of 170 days, about 20% of total cells were positive for Crx, an early photoreceptor marker. On day 200, about 8.5% of the cells expressed the mature rod photoreceptor marker, rhodopsin, as well as cone photoreceptor markers, red/green and blue opsins (8.9% and 9.4%, respectively).A n alternative approach was proposed by the same group based on the use of small molecules. In this method, the chemical inhibitors CKI-7 and SB-431542 that inhibit Wnt and Activin A signaling, respectively, and Y-27632, the Rho-associated kinase inhibitor, which prevents disso-ciation-induced cell death, were used. These molecules were shown to mimic the effects of Dkk1 and LeftyA (Osakada et al. 2009).This strategy, which doesn’t involve the use of recom-binant proteins which are produced in animal or E scherichia coli cells, is more favorable for the gen-eration of cells for future transplantation therapy.I n another study that was published by Meyer et al .(2009), after initial differentiation in sus-pension for 6 days, the aggregates were allowed to attach to laminin–coated culture dishes. After further differentiation as adherent cultures, neu-roepithelial rosettes were formed, which were mechanically isolated and subsequently culti-vated as neurospheres. The authors didn’t use any soluble factors; moreover, they showed that under these conditions, the cells expressed endogenous Dkk1 and Noggin. They also demonstrated that in concordance with the role of bFGF in retinal specifi cation, the inhibition of endogenous FGF-signaling abolished retinal differentiation. Under their differentiation protocol, by day 16, more than 95% of the cells expressed the retinal pro-genitor markers, Pax6 and Rx. The authors dem-onstrated that by day 80 of differentiation, about 19% of all neurospheres contained Crx+ cells and within these Crx+ neurospheres, 63% of all cells express Crx and 46.4% of the cells expressed mature markers, such as recoverin and cone opsin.I n all of the above studies, differentiated cells expressing the retinal markers were obtained; however, the cells were not organized in a three-dimensional retinal structure. In a paper recently published by Eiraku et al. (2011),the authors cul-tured free-fl oating aggregates of mouse ES cells in serum-free medium in the presence of base-ment membrane matrix, Matrigel, that could also94M. Idelson and B. Reubinoffbe substituted with a combination of laminin, entactine and Nodal. Using a mouse reporter ES cell line, in which green fl uorescent protein (GFP) is knocked in at the Rx locus, the authors showed that Rx-GFP+ epithelial vesicles were evaginated from the aggregates after 7 days of differentiation under these conditions. On days 8–10, the Rx-GFP+ vesicles changed their shape and formed optic cup-like structures. The inner layer of these structures expressed markers of the neural retina whereas the outer layer expressed markers of RPE. The authors demonstrated that differen-tiation into RPE required the presence of the adjacent neuroectodermal epithelium as a source of diffusible inducing factors. In contrast, the differentiation into neural retina did not require tissue interactions, possibly because of the intrinsic inhibition of the Wnt-signaling pathway. Eiraku and colleagues showed that the retinal architecture, which was formed within the optic vesicle-like structures, was comparable to the native developing neural retina.R ecently, optic vesicle-like structures were also derived from hESCs and iPSCs using the protocol described above, which is based on iso-lating the neural rosette-containing colonies and culturing them in suspension (Meyer et al. 2011). The cells within the structures expressed the markers of retinal progenitors, and after differen-tiation gave rise to different retinal cell types. It was shown that the ability of optic vesicle-like structures to adopt RPE fate could be modulated by Activin A supplementation. The production of these three-dimensional retinal structures opens new avenues for studying retinal development in normal and pathological conditions.T ransplantation of Pluripotent Stem Cell-Derived Retinal CellsA key step towards future clinical transplanta-tions of hESC-derived RPE and neural retina is to show proof of their therapeutic potential i n vivo. Various animal models of retinal degeneration have been used to evaluate the therapeutic effect of transplanted retinal cells. Human ESC-derived RPE cells were transplanted subretinally to the degenerated eyes of RCS rats. Transplantation of the hESC-derived RPE cells between the RPE and the photoreceptor layer rescued retinal struc-ture and function (Lund et al. 2006; Vugler et al. 2008; Idelson et al. 2009; Lu et al. 2009).The subretinally engrafted hESC-derived RPE cells salvaged photoreceptors in proximity to the grafts as was shown by the measurement of the thick-ness of the ONL, the layer of photoreceptor nuclei, which is an important monitor of photore-ceptor cell survival. The ONL thickness was significantly increased in transplanted eyes in comparison to the degenerated non-treated eyes.I n order to evaluate the functional effect of transplanted cells i n vivo, the electroretinography (ERG) that directly measures the electrical activ-ity of the outer (a-wave) and inner (b-wave) retina in response to light stimulation was used. It was demonstrated that after transplantation of hESC-derived RPE, ERG recordings revealed a signifi -cant preservation of retinal function in the treated eyes as compared to control untreated eyes (Lund et al. 2006; Idelson et al. 2009).The visual func-tion of the animals was also estimated by an optomotor test, which monitors the animal’s refl exive head movements in response to a rotat-ing drum with fi xed stripes. Animals transplanted with hESC-derived RPE showed signifi cantly better visual performance in comparison to con-trol animals (Lund et al. 2006; Lu et al. 2009). The presence of rhodopsin, a major component of photoreceptor outer segments, within the sub-retinaly transplanted pigmented cells suggested that they could perform phagocytosis i n vivo (Vugler et al. 2008; Idelson et al. 2009).B ridging the gap between basic research and initial clinical trials requires immense resources to ensure safety and efficacy. Human ESC-derived RPE cell lines were generated using a current Good Manufacturing Practices (cGMP)-compliant cellular manufacturing process (Lu et al. 2009). Long-term studies analyzing safety and efficacy of transplantation of these GMP-compliant hESC-derived RPE cells revealed that the subretinally transplanted cells survived for a period of up to 220 days and provided prolonged functional improvement for up to 70 days after transplantation. The potential of the hESC-derived。
小学英语颜色教学colours课件
Rainbow color order
Red Orange
Yellow
Rainbow color order
Green Blue
Indigo
Rainbow color order
Violet
The seven colors of a rainbow are arranged in ascending order of wavelength, forming a beautiful spectrum. These seven colors also represent different meanings and emotions.
Teach students to use adjectives to describe the depth, brightness, and other characteristics of colors, such as "deep red", "light green", etc.
Expand vocabulary: brightness, saturation, etc
小学英语颜色教学 colours课件
REPORTING
• Course Introduction and Objectives • Basic knowledge of color • Vocabulary learning and expansion • Sentence Structure and Application • Gamified teaching activity design • Cultural background and cross-
protection.
Purple
Reproduction color prediction apparatus and method
专利名称:Reproduction color prediction apparatusand method发明人:Kosei Takahashi,Osamu Yamada申请号:US10974888申请日:20041028公开号:US20050083346A1公开日:20050421专利内容由知识产权出版社提供专利附图:摘要:A primary color dot gain correction unit corrects the spectral reflectance of each of a plurality of color agents on the basis of the dot quantity set for each color agent. An initial estimated value calculator estimates a mixed color by the KM theoryusing spectral reflectance data corrected by the primary color dot gain correction unit. An ink overlap correction coefficient storage unit stores correction coefficients, which are determined on the basis of errors between the actually measured values of spectral reflectance data of color patches obtained using the plurality of color agents, and estimated values estimated by the initial estimated value calculator based on the dot quantities of the respective color agents on the color patches. An ink overlap correction unit obtains the prediction result of a reproduction color by correcting the spectral reflectance data of the mixed color calculated by the initial estimated value calculator on the basis of the correction coefficients stored in the ink overlap correction coefficient storage unit.申请人:Kosei Takahashi,Osamu Yamada地址:Kanagawa JP,Tokyo JP国籍:JP,JP更多信息请下载全文后查看。
牙科实践中使用的色彩指南说明书
Review articleSHADE GUIDES USED IN THE DENTAL PRACTICERangel Todorov 1, BozhidarYordanov 1, Todor Peev 1, Stefan Zlatev 21) Department of Prosthetic Dental medicine, Faculty of Dental medicine, Medical University - Sofia, Bulgaria.2) Department of Prosthetic Dental medicine, Faculty of Dental medicine, Medical University - Plovdiv, Bulgaria.Journal of IMAB ISSN: 1312-773Xhttps://SUMMARYThe aim of the article is to present a narrative review of the literature related to the different shade guides avail-able for dental practitioners.A literature search was performed in the online databases – PubMed, Science Direct, MedLine and GoogleScholar with the following keywords – shade guide,shade matching, dental, dentistry. Furthermore, a hand search, including “grey” literature and printed resources available to the authors, was conducted. More than 2100unique articles, including the selected keywords, were iden-tified. After a critical review and elimination process, 39 of the papers were included.Dental shade guides are a set of colour standards mimicking the colour of tissues, which dentists aim to re-store – tooth structures, gingival tissues or facial structures in facial prosthodontics - skin, mucosa, eyes etc. They can be systematized based on the following principles – fabri-cation process, the material of manufacturing or restoration and according to the tissues, which are restored.The most commonly used shade guides in the dental practice are structured around Munsell’s 3-dimensional col-our system and are aimed at the restoration of tooth colour.The colour standards for gingival and facial restoration are not routinely utilized since they are aimed at specialists in prosthetic dentistry and facial prosthodontics and are under-developed to some degree in comparison with the aforemen-tioned.The contemporary development in aesthetic restora-tive materials and procedures requires an adequate follow up in the evolution of shade guides since they present the only means of communication between the dental office and laboratory.Keywords: shade guide, shade-matching, dental, den-tistry, colour,INTRODUCTIONShade guides used in dentistry are comprised of a set of standard or individually fabricated colour standards,which mimic the colour of dental structures [1]. They are used to visually determine a colour match with natural teeth [2 – 4]. Shade guides can be prefabricated or manufactured individually [5, 6]. Another principle for their classification is based on the material, from which they are made – ce-ramic based, composite resin based or acrylic based. Depend-ing on the structures of interest, shade guides can be divided into groups for tooth or gingival colour matching or the ones used in facial prosthodontics [7 – 10]. The last two are not routinely used in dental practice and are underdeveloped to some degree since their usage is oriented towards more spe-cialized practitioners.As a general requirement, shade guides for dental structures should represent the full spectrum of colours natu-rally occurring in the human dentition. They also need to be logically constructed and the tabs ordered in a way that enables easy usage. As a general principle, each shade tab has an incisal, middle and cervical part, which differ based on the colour density and translucency [11, 12].Colour determination is an important point in all di-rections of prosthetic dentistry [13, 14] and the clinical data collected are directed to the dental laboratory at the plan-ning stage of structures [15, 16].MA TERIALS AND METHODSA literature search was performed in the online databases – PubMed, Science Direct, Medline and Google Scholar with the following keywords – shade guide, shade matching, dental, dentistry. Furthermore, a hand search, in-cluding “grey” literature and printed resources available to the authors, was conducted. More than 2100 unique articles,including the selected keywords, were identified. After a criti-cal review and elimination process, 39 of the papers were included.Development of colour standardsIn 1931 Clark was the first person who attempted to scientifically systematize the colours occurring in the natu-ral dentition. In order to achieve this, he arranged 703 shade tabs, which were divided into two groups representing the gingival or incisal area of the tooth’s crown. Those were grouped into 19 degrees for value, 6 for Chroma and 3 for hue, thus the shade guide - “Color Indicator”, which con-sists of 60 shade tabs was created.Hall creates a shade guide for porcelain restorations,using the principles, which later became the base for the crea-tion of Vita 3D Master. There are shade tabs arranged accord-ing to the value in 3 groups – light moderate and dark. Each of those has 9 tabs positioned vertically based on their Chroma and horizontally based on the hue in 3 rows with different tints [17].https:///10.5272/jimab.2020262.3168In 1939 VITA Zahnfabrik created its first shade guide - the result of an epidemiological study for determination of tooth shades occurring in the natural dentition. In 1956VITA Lumin Vacuum Shade Guide was introduced and marked the first attempt at creating a unified colour stand-ard [18, 19]. Since then, numerous dental material manufac-turers propose different shade guides [20, 21, 22]. These create a bigger diversity but also confuse practising dentists [23,24, 25, 26].In 1976-1978 CIE creates a new colour system called CIELab, classifying all the shades mathematically. This ena-bles the easy and accurate definition of different colours us-ing numeric representation. One of the first clinical impli-cations of the system lies in the development of the VITA 3D Master shade guide.There are 3 main shade guides used in the daily prac-tice, namely the ones developed by VITA, Bioform by DeTrey and Chromascop by Ivoclar. They are based on Munsell’s principles, which divide the colour space into three dimensions – name of the colour (hue), the density of the colour (Chroma), and vitality (value) [8, 12].Shade guides manufactured from ceramic materials Vita Classical; Vita LuminThe colour space in Vita Classical is divided into four groups – A, B, C and D based on the dominating HUE (name of the colour). In group “A” these are red and brown, in group B - red and yellow, in group C – grey and in group D red and grey. Each of the “letter” groups has subdivisions in-dexed with Arabic numbers ranging from 1 to 4, making the total number of tabs 16. (Fig.1) With the increase of the number, the Chroma increases, while at the same time, the value decreases [27].Fig. 1. Vita Classical shade guideThe manufacturer proposes an alternative tab order based on the value, which eliminates the aforementioned structure and creates an arrangement in the following man-ner: B1, A1, B2, D2, A2, C1, C2, D3, A3, D4, B3, A3.5, B4,C3, A4, and C4. In recent years a modification of the shade guide is proposed. Three new tabs for bleached teeth are in-cluded – 0M1, 0M2, 0M3 [12].Vita 3D MasterThis shade guide consists of 26 tabs arranged in 5groups according to their value. (Fig. 2) Inside the groups,the shade tabs are ordered along two axes – vertical accord-ing to Chroma and horizontal based on hue. The first grouphas two tabs, the second, third and fourth have seven each,while the fifth has three. Each shade tab has three labels – a number ranging from 1 to 5, showing the group and value level, which decreases as the number increases. The letter M defines a mean colour tone, which is representative for the given value, while R and L are arranged relative to the median colour estimate. The last numeric indicator – 1, 1.5,2, 2.5 and 3 shows the Chroma levels that are increasing in-crementally. Three additional tabs are added for bleached teeth – 0M1, 0M2 and 0M3, the latter denoting the valueof 0, three levels of Chroma and mean colour tone (M) [18].Fig. 2. Vita 3D Master shade guideThere are several improvements of Vita 3D Master shade guide in comparison to Vita Classical:• There is a wider value range;• The red spectra has increased range;• The shade tabs are more equally distributed in the colour space;• The group distribution is better and more con-cise [12].Vita Linear guideIn 2006 as a natural development, a new shade guide was proposed – VITA Linearguide. It is based entirely on 3D Master and contains all of its tabs, which are distributed in an innovative manner. Shade tabs are arranged according to their value. The shade guide is further subdivided into six “sub-guides” – one main based on value and five acces-sories for Chroma and Hue. (Fig. 3) The latter is an attempt to overcome the disadvantages of the previous generations of shade guides. With Vita Classical, shade registration is a one-step process. This presents a difficulty since the correct colour should be chosen among all of the shade guide’s tabs.Shade matching with Vita 3D Master is a three-step process,which also leads to difficulties in the correct shade determi-nation [12, 28].Fig. 3. Vita Linear GuideWith Vita Linearguide shade registration is a two-step process that is easier and quicker. First, the Value-tab from the main (Value) sub-guide is chosen, followed by the ac-tual (Chroma/Hue) shade tab from one of the accessory sub-guides. The process is easier and results in a more precise and quicker shade matching since in each stage, the choice is made between a small number of shade tabs [12].Vita Bleachguide 3D MasterThese colour standards are used for shade matching or evaluation during tooth whitening procedures. The shade guide has 15 tabs which correspond to 29 shades approved by ADA. (Fig. 4) The application of a specialized shade guide aids in the more realistic evaluation of the tooth whit-ening process, while also contributing to the communica-tion between the dentist and the patient [4, 29, 30].Fig. 4.Vita Bleach guideThe shade guide follows the logic of tab arrangement used in 3D Master and uses the Value dimension for deter-mining its structure. The numeric indications ranging from 0 to 5 denote the given Value of the shade tab, while the letter indicator, ranging from M1 to M3, and correspond to the Hue dimension similar to the M group in 3D Master [31].Chromascop (Ivoclar Vivadent, Amherst, NY, USA)In this shade guide, the colour space is distributed in five groups based on the Hue dimension. The colour-cod-ing is as follows:•In group 100 - white;•In group 200 - yellow;•In group 300 – grey;•In group 400 – grey;•in group 500 – dark brown.Each group has five shade tabs. With the increase in number the chroma of the colour increases and at the same time the value decreases. The Shade guide consists of 20 tabs. The number for each of them is a combination of he subgroup and the other two colour dimensions. As an addi-tion, accessory shade tabs are added for bleached teeth – 010, 020, 030 and 040 [12].BioformThe overall number of tabs in this shade guide is 24. The colour space is divided into four groups based on the dominating hue – red-brown, yellow, red-grey and grey [12].Shade guides manufactured from composite resin materialsMost of the manufacturers support the claim that the colour of their composite resin or acrylic materials correspond to the ceramic colour standards [32, 33]. Nevertheless almost all of them have specific shade guides supplied with their ma-terials [12, 34]. The shade tabs made from composite resin materials can be with one or multiple layers depending on the manufacturing process. Sometimes the manufacturers sup-ply “protocols” for mixing of different enamel and dentin masses so that the desired shade can be achieved [35].The shade tab’s order is analogous to the logic used in the ceramic based shade guides. Disinfecting solutions, heat, polishing and storage time can greatly affect compos-ite resin materials and should be taken into consideration when using these shade guides [27, 36].Among the more widely used shade guides from this group are Esthetic-X, TetricEvo Ceram and Venus.Esthetic-X (Dentsply)This shade guide consists of 16 shade tabs with the addition of a universal, universal-light, light-grey and yel-low-grey and three tabs for bleached dental tissues [12].TetricEvo Ceram (Ivoclar Vivadent)The shade guide consists of 11 colour standards, 3 dentin colours, one tab for evaluation of translucency and 4 for bleached teeth [12].Venus (HereusKulzer)The colour standards are arranged in three groups -15 standard shade tabs, three for evaluation of translucency and two for bleached structures [12].Shade guides for soft tissues in the cranial-facial areaThe use of a universal pink shade cannot reproduce the colour characteristics of gingival tissues [10].The colour space in gingival tissues has a bigger spec-tre than that for dental structures, where the value and hue have wider borders and the Chroma narrower. The colour of the restoration’s base, be it fixed or removable, can be re-produced using a standard or individually manufactured shade guide. With the usage of the later individual charac-teristics of soft tissues can be highlighted [12].Most frequently used standard shade guides for soft tissues in dentistry are Lucitone 199, Dentsply Trubute and Ivoclar Plus Gingiva indicator [37].Alongside the main characteristics of colour – value, hue and Chroma, important aspects of shade matching pro-cedures for soft tissues include the size, shape and thickness of the restorations as well as the ethnic background of the patient.Shade guides for facial prosthodonticsThe accurate recreation of individual colour charac-teristics of soft tissues in the facial area requires the usage shade guides developed for facial prostheses. Although the colour range of human skin is wide, there are no universally accepted shade guides for facial prosthodontics [9].Over proposes a 7step silicon guide and points out that one of the most important aspects in such restorations is the construction’s depth [37]. Godoy develops an acrylic guide consisting of 7 shade tabs [7].Clinically (individually) fabricated shade guidesIt is not always possible to determine the colour match between the natural dentition and commercially avail-able shade guides [12, 26]. The individually fabricated shade guides have the advantage that they are made from the ma-terial which will be used for the final restoration [33]. As an additional advantage, there is the possibility to create sev-eral options with variable thickness, surface structure, glaze and individualization of different colour characteristics.Colour determination is an important factor in satis-fying the aesthetic medical-biological indicator of different types of prosthetic treatments in both adult and paediatric patients [38, 39, 40].CONCLUSIONColour dimensions of dental structures and their re-construction have its place in achieving a harmonious den-tition. Shade guides for soft tissues and facial prosthodon-tics should be further developed. The accurate recreation of colour dimensions in an important step for achieving a har-monious dentition. The dentist has a plethora of options con-cerning shade matching for hard tooth substances (i.e. white aesthetics). The development of shade guides follows the logical and technical progress, with its peak – the creation of Vita Linear Guide.Soft tissue aesthetics (i.e. pink aesthetics) and the rec-reation of skin colour is still an underdeveloped area with little to none existing shade guides. Further research is needed in order to create a universally accepted and appli-cable colour standard for these cases.1. Clark EB. Tooth color selection. J Am De nt Assoc. 2019 Jun;20(6): 1065-73. [Crossref]2. Hadjieva H, Dimova M. Visuali-zation of the clinical observations in the different stages of the dental pros-thetic treatment process. Pharmacolo-gyonline. 2008 Aug 30;2:99-104. [Internet]3. Hadjieva H, Dimova M, Peev T. [Clinical procedures contributing to the esthetic result by total denture treatment.] Zabolekarskipregled. 2006; 2:108-12. [in Bulgarian]4. Joiner A. Tooth colour: a review of the literature. J De nt. 2004; 32(Suppl 1):3-12. [PubMed] [Crossref]5. Aleksandrov S, Todorov G, Hristov S. [Embellishment of prognos-tic constructions for precise asthetical planning.] Proce e dings of SUB-Plovdiv. 2011;14:10-2. [in Bulgarian]6. Todorov R, Todorov G. [Systems for registration and devices for color measurement.]Proce e dings of SUB-Plovdiv. 2012; 14:5-9. [in Bulgarian]7. Godoy AJ, Lemon JC, Nakamura SH, King GE. A shade guide for acrylic resin facial prostheses. J Prosthet Dent. 1992 Jul;68(1):120-2. [PubMed] [Crossref]8. Pustina-K rasniqi T, Shala K, Staka G, Bicaj T, Ahmedi E, Dula L. Lightness, chroma, and hue distribu-tions in natural teeth measured by a spectrophotometer. Eur J De nt. 2017 Jan-Mar;11(1):36-40. [PubMed] [Crossref]9. Mehta S, Nandeeshwar DB. A spectrophotometric analysis of extra-oral aging conditions on the color sta-bility of maxillofacial silicone. J In-dian Prosthodont Soc. 2017 Oct-Nov; 17(4):355-60. [PubMed] [Crossref]10. Hyun H-K, Kim S, Lee C, Shin TJ, Kim Y-J. Colorimetric distribution of human attached gingiva and alveo-lar mucosa. J Prosthet Dent. 2017 Feb; 117(2):294-302. [PubMed] [Crossref]11. Hammad IA. Intrarater repea-tability of shade selections with two shade guides. J Prosthe t De nt. 2003 Jan;89(1):50-3. [PubMed] [Crossref]12. Paravina RD. Performance as-sessment of dental shade guides. J Dent. 2009; 37(Supl 1):e15-20. [PubMed] [Crossref]13. Hadjieva H, Dimova M, Peev T. Total rehabilitation by edentulous pa-tients with irregularity of the alveolar ridges. J of IMAB. 2005; 11(2):54-56. [Internet]14. Dimova M, Hadjieva H, Peev T. [Oral clinical patient record for obser-vation data of edentulous patients.] De ntal me dicine. 2009; 1: 34-40. [in Bulgarian]15. Dimova M, Hadjieva H, Peev T. [Documented data transferand direc-tions between clinicand laboratory in total prosthetics.] Zabolekarski pregled. 2006; 2: 103-107. [in Bulgar-ian]16. DimovaM, Jankova M, Peev T. [An inquiry into the laboratory shap-ing in fabrication of complete den-tures.] Proble ms of de ntal me dicine. 2008; XXXIV(II):43-49. [in Bulgarian]17. Sproull RC. Color matching in dentistry. part I. The three-dimensional nature of color. J Prosthet Dent. 1973 Apr;29(4):416-24. [PubMed] [Crossref]18. Brewer JD, Wee A, Seghi R. Ad-vances in color matching. De nt Clin North Am. 2004 Apr;48(2):341-58. [PubMed]19. Rao D, Joshi S. Evaluation of Natural Tooth Color Space of the In-dian Population and Its Comparison to Manufacturer’s Shade Systems. Conte mp Clin De nt. 2018 Jul-Sep; 9(3):395–399. [PubMed]20. Ho DK, Ghinea R, Herrera LJ, Angelov N, Paravina RD. Color Range and Color Distribution of Healthy Hu-man Gingiva: a Prospective Clinical Study. Sci Rep. 2015 Dec 22;5:18498. [PubMed] [Crossref]21. Gomez-Polo C, Gomez-Polo M, Martinez Vazquez de Parga JA, CeleminVinuela A. Study of the most frequent natural tooth colors in the Spanish population using spectropho-tometry. J Adv Prosthodont. 2015 Dec;7(6):413-22. [PubMed]22. Paolone G, Orsini G, Manauta J, Devoto W, Putignano A. Composite shade guides and color matching. Int J Esthe t De nt. 2014 Summer;9(2): 164-82. [PubMed]23. Dimova M, Peev T. [Methodi-cal approach for evaluation of the pa-tient satisfaction with prosthetic treat-REFERENCES:ment with provisional crowns andbridges. Social me dicine. 2003; 1:29-30. [in Bulgarian]24. Dimova M, Peev T. [Satisfac-tion of patients with prosthetic treat-ment with laboratory provisional con-structions (study results).] Social medi-cine. 2003;4: 30-32. [in Bulgarian]25. Dimova M, Peev T. [Method forlaboratory determination of the shapeof the upper frontal teeth in total pros-thetic treatment (a pre-release mes-sage).] Scie ntific Re se arche s of theUnion of Scie ntists in Bulgaria –Plovdiv 2007, Se rie s Â. Natural Sci-e nce s and the Humanitie s. 2008; IX:165-170. [in Bulgarian]26. Paravina R, Stankovic D,Aleksov L, Mladenovic D, Ristic K.Problems in standard shade matchingand reproduction procedure in den-tistry: a review of the state of the art.Facta Unive rsitatis, Se rbia. 1997;4(1):12-6.27. Udiljak Z, Illes D, K nezovicZlataric D, Celic R. Effect of clinicalexperience on the shade matching ac-curacy in different dental occupationalgroups. Acta Stomatol Croat. 2018Jun;52(2):132-139.[PubMed]28. Yuan JC-C, Brewer JD, MonacoEA, Davis EL. Defining a natural toothcolor space based on a 3-dimensionalshade system. J Prosthe t De nt. 2007Aug;98(2):110-9. [PubMed]29. Joiner A, Luo W. Tooth colourand whiteness: A review. J Dent. 2017Dec;67:3-10. [PubMed]30. MiyajiwalaJS, K heurMG,Patankar AH, LakhaTA. Comparisonof photographic and conventionalmethods for tooth shade selection: Aclinical evaluation. J Indian Prostho-dont Soc. 2017 Jul-Sep;17(3):273-281.[PubMed]31. Paravina RD, Ontiveros JC,Powers JM. Curing-dependent changesin color and translucency parameter ofcomposite bleach shades. J Esthet Re-stor D e nt. 2002;14(3):158-66.[PubMed]32. Baratieri LN, Araujo E,Monteiro S, Jr. Color in natural teethand direct resin composite restora-tions: essential aspects. Eur J Esthe tDent. 2007 Summer;2(2):172-86.[PubMed]33. Ferracane JL. Current trends in dental composites. Crit Rev Oral Biol Med . 1995; 6(4):302-18. [PubMed ]34. Yap AU. Color attributes and accuracy of vita-based manufacturers’shade guides. Oper Dent. 1998 Sep-Oct;23(5):266-71. [PubMed ]35. Ragain JC. Jr, Johnston WM.Color acceptance of direct dental re-storative materials by human observ-ers. Color Res Appl . 2000; 25(4):278-85. [Crossref ]Address for correspondence:Rangel TodorovFaculty of dental medicine, MU-Sofia 1, G. Sofijski Str.,1431 Sofia, Bulgaria.E-mail: *****************,36. Fay RM, Servos T, Powers JM.Color of restorative materials after staining and bleaching. Ope r De nt .1999 Sep-Oct;24(5):292-6. [PubMed ]37. Over LM, Andres CJ, Moore BK, Goodacre CJ, Munoz CA. Using a colorimeter to develop an intrinsic sili-cone shade guide for facial prostheses.J Prosthodont . 1998 Dec;7(4):237-49.[PubMed ]38. Dimitrova D. [Fixed prosthetic treatment in childhood – functionalaspects.] [dissertation]. Medical Uni-versity – Varna. 2018. 189 p. [in Bul-garian]39. Dimitrova D. Questionnaire survey of removable prosthetic treat-ment in children. MedInform . 2019;6(1):980- 986. [Crossref ]40. Dimitrova D. Study of patient satisfaction with prosthetic treatment.MedInform . 2018; 5(3):914-919.[Crossref ]Ple ase cite this article as: Todorov R, Yordanov B, Peev T, Zlatev S. Shade guides used in the dental practice. J of IMAB. 2020 Apr-Jun;26(2):3168-3173. DOI: https:///10.5272/jimab.2020262.3168Received: 29/06/2019; Published online: 01/06/2020。
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of input system are equivalent to the color matching function of the display primaries [ 41, the spectral sensitivities of the real system do not agree with the color matching functions. Thus, we employed a simple 3 x 3 matrix to perform color correction of the input system. It should be realized that this color correction method is not new, having been applied for televisions [ 11. Optimizing the correction matrix of the electronic endoscope for clinical use is, however, a novel application. Such optimization necessitates having the statistical distribution of spectral reflectance of the real mucous membrane, and fortunately, since this data was in-hand, having been previously measured by a specially made endoscopic system for spectral photometry [ 5 1, we utilized it to optimize the correction matrix. In the following sections, we describe the theory of the optimization method and then present computer simulation results that confirm the effectiveness of the proposed method.
ቤተ መጻሕፍቲ ባይዱ
Fig. 1. Diagram of a typical electronic endoscope. 0030-4018/95/$09.50 0 1995 Elsevier Science B.V. All rights reserved SsDrOO30-4018(94)00531-l
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H. Haneishi et al. /Optics Communications114 (1995) 57-63
and diagnosis possible. In fact, if numerous endostopic images are collected to build a database, this significantly enhances such analysis and diagnosis. Towards this end, however, a crucial problem exists, i.e., the reliability and stability of color reproduction for the generated endoscopic images must be improved. The associated transmission path for color information is quite complex. Namely, the spectral information from the object is initially reduced to three values by weighting and integrating with the spectral sensitivities of the input system for each color. If necessary, these values are gain-controlled at the video processor, and then excite the CRT’s red, green, and blue phosphors. At this time the light intensity is not linear to the input signals because of the gamma characteristics of the CRT. Consequently, the reproduced color has a strong dependence on the following: (i) the spectral characteristics of the white light source, (ii) the spectral transmittance of the color filters, (iii) the gain at the video controller, (iv) the spectral distribution of the three phosphors, and (v) the gamma characteristics of the CRT. Obviously the reproduced colors in the computeraided image analysis and diagnosis should be close to the true color of the object and should not depend on the image acquisition site or time. The electronic endoscope, though, has an inherent limitation in that it is impossible to retrieve the spectral information of the object. To mitigate this drawback, a next-best solution for obtaining accurate color reproduction involves using a calorimetric color reproduction in which tristimulus values of the object are employed [ 11. In conventional endoscopic systems, however, such color reproduction is not guaranteed. This problem led to the present paper which describes a new color correction method for calorimetric color reproduction of electronic endoscope images. The proposed method is comprised of a two-step calibration procedure, where an input system calibration is first done to obtain the ideal R, G, B tristimulus values, which are determined by the chromaticities of the display primaries. Next, the CRT’s gamma characteristics are calibrated to reproduce these tristimulus values. The latter calibration is accomplished using either tabulated values or corresponding analytic functions [ 2,3 1. Here, we focus on the former calibration. Although the ideal tristimulus values can be obtained when the spectral sensitivities
15 January 1995
OPTICS COMMUNICATIONS
ELSEVIER
Optics Communications 114 (1995) 57-63
Color correction for calorimetric color reproduction in an electronic endoscope
1. Introduction The utilization of electronic endoscopes vice conventional optical fiber scopes has recently gained much popularity in the medical profession. The typical electronic endoscope (Fig. 1) incorporates an integrated CCD area sensor at the head of the scope which can image gastric or intestinal surfaces. The light source part consists of a white light source (xe-
Received 5 April 1994; revised manuscript received 23 August 1994
Abstract A color correction method is presented for application in an electronic endoscope. This method performs color correction in the endoscope’s input system using a simple 3 x 3 matrix to reproduce a calorimetrically correct color. The correction matrix is optimally designed using about 300 actual spectral reflectances of the gastric mucous membrane. The effectiveness of the proposed correction method is demonstrated both numerically and visually through computer simulation.