PP58_SDS_MedChemExpress
雷帕霉素-SDS-MedChemExpress
Inhibitors, Agonists, Screening LibrariesSafety Data Sheet Revision Date:Jan.-07-2019Print Date:Jan.-07-20191. PRODUCT AND COMPANY IDENTIFICATION1.1 Product identifierProduct name :RapamycinCatalog No. :HY-10219CAS No. :53123-88-91.2 Relevant identified uses of the substance or mixture and uses advised againstIdentified uses :Laboratory chemicals, manufacture of substances.1.3 Details of the supplier of the safety data sheetCompany:MedChemExpress USATel:609-228-6898Fax:609-228-5909E-mail:sales@1.4 Emergency telephone numberEmergency Phone #:609-228-68982. HAZARDS IDENTIFICATION2.1 Classification of the substance or mixtureGHS Classification in accordance with 29 CFR 1910 (OSHA HCS)Flammable liquids (Category 4), H2272.2 GHS Label elements, including precautionary statementsPictogram No data availableSignal word WarningHazard statement(s)H227 Combustible liquid.Precautionary statement(s)P210 Keep away from heat ⁄sparks ⁄open flames ⁄hot surfaces. - No smoking.P280 Wear protective gloves ⁄ protective clothing ⁄ eye protection ⁄ face protection.P370 + P378 In case of fire: Use dry sand, dry chemical or alcohol-resistant foam for extinction.P403 + P235 Store in a well-ventilated place. Keep cool.P501 Dispose of contents ⁄ container to an approved waste disposal plant.2.3 Other hazardsNone.3. COMPOSITION/INFORMATION ON INGREDIENTS3.1 SubstancesSynonyms:SirolimusFormula:C51H79NO13Molecular Weight:914.17CAS No. :53123-88-94. FIRST AID MEASURES4.1 Description of first aid measuresEye contactRemove any contact lenses, locate eye-wash station, and flush eyes immediately with large amounts of water. Separate eyelids with fingers to ensure adequate flushing. Promptly call a physician.Skin contactRinse skin thoroughly with large amounts of water. Remove contaminated clothing and shoes and call a physician.InhalationImmediately relocate self or casualty to fresh air. If breathing is difficult, give cardiopulmonary resuscitation (CPR). Avoid mouth-to-mouth resuscitation.IngestionWash out mouth with water; Do NOT induce vomiting; call a physician.4.2 Most important symptoms and effects, both acute and delayedThe most important known symptoms and effects are described in the labelling (see section 2.2).4.3 Indication of any immediate medical attention and special treatment neededTreat symptomatically.5. FIRE FIGHTING MEASURES5.1 Extinguishing mediaSuitable extinguishing mediaUse water spray, dry chemical, foam, and carbon dioxide fire extinguisher.5.2 Special hazards arising from the substance or mixtureDuring combustion, may emit irritant fumes.5.3 Advice for firefightersWear self-contained breathing apparatus and protective clothing.6. ACCIDENTAL RELEASE MEASURES6.1 Personal precautions, protective equipment and emergency proceduresUse full personal protective equipment. Avoid breathing vapors, mist, dust or gas. Ensure adequate ventilation. Evacuate personnel to safe areas.Refer to protective measures listed in sections 8.6.2 Environmental precautionsTry to prevent further leakage or spillage. Keep the product away from drains or water courses.6.3 Methods and materials for containment and cleaning upAbsorb solutions with finely-powdered liquid-binding material (diatomite, universal binders); Decontaminate surfaces and equipment by scrubbing with alcohol; Dispose of contaminated material according to Section 13.7. HANDLING AND STORAGE7.1 Precautions for safe handlingAvoid inhalation, contact with eyes and skin. Avoid dust and aerosol formation. Use only in areas with appropriate exhaust ventilation.7.2 Conditions for safe storage, including any incompatibilitiesKeep container tightly sealed in cool, well-ventilated area. Keep away from direct sunlight and sources of ignition.Recommended storage temperature:Powder-20°C 3 years4°C 2 years* The compound is unstable in solutions, freshly prepared is recommended.Shipping at room temperature if less than 2 weeks.7.3 Specific end use(s)No data available.8. EXPOSURE CONTROLS/PERSONAL PROTECTION8.1 Control parametersComponents with workplace control parametersThis product contains no substances with occupational exposure limit values.8.2 Exposure controlsEngineering controlsEnsure adequate ventilation. Provide accessible safety shower and eye wash station.Personal protective equipmentEye protection Safety goggles with side-shields.Hand protection Protective gloves.Skin and body protection Impervious clothing.Respiratory protection Suitable respirator.Environmental exposure controls Keep the product away from drains, water courses or the soil. Cleanspillages in a safe way as soon as possible.9. PHYSICAL AND CHEMICAL PROPERTIES9.1 Information on basic physical and chemical propertiesAppearance White to off-white (Solid)Odor No data availableOdor threshold No data availablepH No data availableMelting/freezing point No data availableBoiling point/range No data availableFlash point No data availableEvaporation rate No data availableFlammability (solid, gas)No data availableUpper/lower flammability or explosive limits No data availableVapor pressure No data availableVapor density No data availableRelative density No data availableWater Solubility No data availablePartition coefficient No data availableAuto-ignition temperature No data availableDecomposition temperature No data availableViscosity No data availableExplosive properties No data availableOxidizing properties No data available9.2 Other safety informationNo data available.10. STABILITY AND REACTIVITY10.1 ReactivityNo data available.10.2 Chemical stabilityStable under recommended storage conditions.10.3 Possibility of hazardous reactionsNo data available.10.4 Conditions to avoidNo data available.10.5 Incompatible materialsStrong acids/alkalis, strong oxidising/reducing agents.10.6 Hazardous decomposition productsUnder fire conditions, may decompose and emit toxic fumes.Other decomposition products - no data available.11.TOXICOLOGICAL INFORMATION11.1 Information on toxicological effectsAcute toxicityClassified based on available data. For more details, see section 2Skin corrosion/irritationClassified based on available data. For more details, see section 2Serious eye damage/irritationClassified based on available data. For more details, see section 2Respiratory or skin sensitizationClassified based on available data. For more details, see section 2Germ cell mutagenicityClassified based on available data. For more details, see section 2CarcinogenicityIARC: No component of this product present at a level equal to or greater than 0.1% is identified as probable, possible or confirmed human carcinogen by IARC.ACGIH: No component of this product present at a level equal to or greater than 0.1% is identified as a potential or confirmed carcinogen by ACGIH.NTP: No component of this product present at a level equal to or greater than 0.1% is identified as a anticipated or confirmed carcinogen by NTP.OSHA: No component of this product present at a level equal to or greater than 0.1% is identified as a potential or confirmed carcinogen by OSHA.Reproductive toxicityClassified based on available data. For more details, see section 2Specific target organ toxicity - single exposureClassified based on available data. For more details, see section 2Specific target organ toxicity - repeated exposureClassified based on available data. For more details, see section 2Aspiration hazardClassified based on available data. For more details, see section 212. ECOLOGICAL INFORMATION12.1 ToxicityNo data available.12.2 Persistence and degradabilityNo data available.12.3 Bioaccumlative potentialNo data available.12.4 Mobility in soilNo data available.12.5 Results of PBT and vPvB assessmentPBT/vPvB assessment unavailable as chemical safety assessment not required or not conducted.12.6 Other adverse effectsNo data available.13. DISPOSAL CONSIDERATIONS13.1 Waste treatment methodsProductDispose substance in accordance with prevailing country, federal, state and local regulations.Contaminated packagingConduct recycling or disposal in accordance with prevailing country, federal, state and local regulations.14. TRANSPORT INFORMATIONDOT (US)This substance is considered to be non-hazardous for transport.IMDGThis substance is considered to be non-hazardous for transport.IATAThis substance is considered to be non-hazardous for transport.15. REGULATORY INFORMATIONSARA 302 Components:No chemicals in this material are subject to the reporting requirements of SARA Title III, Section 302.SARA 313 Components:This material does not contain any chemical components with known CAS numbers that exceed the threshold (De Minimis) reporting levels established by SARA Title III, Section 313.SARA 311/312 Hazards:No SARA Hazards.Massachusetts Right To Know Components:No components are subject to the Massachusetts Right to Know Act.Pennsylvania Right To Know Components:No components are subject to the Pennsylvania Right to Know Act.New Jersey Right To Know Components:No components are subject to the New Jersey Right to Know Act.California Prop. 65 Components:This product does not contain any chemicals known to State of California to cause cancer, birth defects, or anyother reproductive harm.16. OTHER INFORMATIONCopyright 2019 MedChemExpress. The above information is correct to the best of our present knowledge but does not purport to be all inclusive and should be used only as a guide. The product is for research use only and for experienced personnel. It must only be handled by suitably qualified experienced scientists in appropriately equipped and authorized facilities. The burden of safe use of this material rests entirely with the user. MedChemExpress disclaims all liability for any damage resulting from handling or from contact with this product.Caution: Product has not been fully validated for medical applications. For research use only.Tel: 609-228-6898 Fax: 609-228-5909 E-mail: tech@Address: 1 Deer Park Dr, Suite Q, Monmouth Junction, NJ 08852, USA。
蛋白裂解液配方
蛋白裂解液配方
蛋白裂解液是一种在生物学和生物化学实验中常用的试剂，用于裂解或溶解细胞，释放细胞内的蛋白质。
以下是一个基本的蛋白裂解液的常见配方，但请注意，具体的配方可能因实验目的和样品类型而有所不同。
常见的蛋白裂解液配方：
1.RIPA缓冲液：
•50 mM Tris-HCl pH 7.4
•150 mM NaCl
•1% Triton X-100
•0.5% 钠脱氧胆酸
•0.1% SDS
• 1 mM EDTA
• 1 mM PMSF（苯甲磺酰氟）
注：可以根据实验需求加入蛋白酶抑制剂、磷酸酯酶抑制剂等。
emmli裂解缓冲液：
•2% SDS
•10% 甘油
•60 mM Tris-HCl pH 6.8
•100 mM DTT（二硫代硫酸钠）
注：此为常用于SDS-PAGE的裂解液，可用于蛋白质的电泳分析。
3.蛋白裂解酶消化液：
•50 mM Tris-HCl pH 8.0
•150 mM NaCl
• 1 mM EDTA
•1% Triton X-100
•0.5% NP-40
•蛋白酶（如胰蛋白酶）或其他特定的蛋白酶
注：此液体适用于进行蛋白酶的部分或完全消化。
请注意，这些配方中的成分可以根据具体的实验需求进行调整。
在制备蛋白裂解液时，应该遵循严格的实验室规范，并根据样品的特性和实验目的选择合适的裂解液。
此外，对于某些特定的实验，可能需要添加蛋白酶抑制剂、磷酸酯酶抑制剂等以保护蛋白质不被降解。
sds-聚丙烯酰胺凝胶电泳实验报告.
sds-聚丙烯酰胺凝胶电泳实验报告.
实验目的：通过SDS-聚丙烯酰胺凝胶电泳技术，分离蛋白质并测定分子量。
实验原理：SDS-聚丙烯酰胺凝胶电泳是一种分离蛋白质的标准方法，可对不同来源和大小的蛋白质进行分离和鉴定。
在本实验中，通过将蛋白质溶解于含有SDS和2-巯基乙醇等条件下的试样缓冲液中，以使蛋白质带有几乎相同的负电荷，通过电泳使蛋白质在聚丙烯酰胺凝胶中以大小不同的分子量在电场作用下移动，最终被染色，进行分离鉴定。
实验步骤：
1.准备实验材料：SDS-PAGE电泳装置、试样缓冲液、聚丙烯酰胺凝胶、蛋白质样品、电泳缓冲液等。
2.制备SDS-PAGE电泳凝胶：按照说明书，将聚丙烯酰胺凝胶拆封并放置在实验室温度下一段时间，使其柔软。
3.制备电泳缓冲液：按照说明书，取适量的Tris、glycine和SDS等物质，配制电泳缓冲液，使其达到所需浓度。
4.制备试样缓冲液：按照说明书，取适量的Tris、SDS和2-巯基乙醇等物质，配制试样缓冲液。
5.制备样品：取适量的蛋白质样品，在试样缓冲液中煮沸一段时间，使蛋白质分子展开。
6.装样：将制备好的样品加入聚丙烯酰胺凝胶电泳装置样品槽中。
7.电泳：按照说明书，调整电泳缓冲液pH值和电泳电压等条件，开启电泳装置，进行电泳操作。
8.染色：将电泳板取出，进行荧光染色或银染色法。
9.图像处理：使用图像分析系统或其他软件，进行蛋白质条带的分析和图像处理。
实验结果：经过SDS-PAGE电泳后，样品中的蛋白质在凝胶中呈现出大小不同的分子量条带，通过荧光染色或银染色可以清晰地看到分离的蛋白质条带。
根据蛋白质的分子量大小，可以判断样品中含有哪些蛋白质。
SDS-聚丙烯酰胺凝胶电泳
做标准曲线
以标准蛋白的迁பைடு நூலகம்率为横坐标，以其对应的相对分子质 量的对数为纵坐标 ，制作标准曲线，
图2：标准曲线
样品蛋白相对分子质量的确定：
根据未知样品迁移率，利用标准曲线计算样品相对 分子质量。
表2：样品蛋白的迁移率及相对分子质量
样品蛋白 移动距离cm 溴酚蓝指示剂前 沿距离cm 相对迁移率 LgMr Mr 1 2 3 4 5
注意： 染色可以重复使用，染色完成以后回收入试剂瓶，染色 后准备配制漂洗液。每组150mL。
结果
图1：电泳图谱
计算迁移率
精确测量溴酚蓝染料 和各种标准蛋白移动的距 离，根据公式，计算各种 蛋白质的相对迁移率。
迁移率= 蛋白质样品的移动距离(cm) 染料的移动距离(cm)
表1：标准蛋白的迁移率(Rf值)
当蛋白质的分子量在15～200KD之间时，电泳迁移率与分子量的对 数呈直线关系，符合下列方程式
其中：MW为蛋白质分子量，Mr为相对迁移率，b为斜率，k为截距。在条 件一定时，b和K均为常数。 Mr=
lgMW=-b· Mr+K
蛋白质样品的迁移距离(cm) 染料的迁移距离(cm) 若将已知分子量的标准蛋白质 的迁移率对分子量的对数作图， 可获得一条标准曲线。未知蛋 白质的相同条件下进行电泳， 根据它的电泳迁移率即可在标 准曲线上求得分子量。
分离胶和浓缩胶
浓缩胶和分离胶的配制
组分 蒸馏水/ml 分离胶(下层胶)缓冲溶液(pH8.8)/mL 浓缩胶(上层胶)缓冲溶液(pH6.8)/mL 凝胶贮备液/mL TEMED/mL 10%过硫酸胺/mL 总体积、ml 12.5%分离胶 5.3 4.0 － 6.7 0.002 0.2 16 5%浓缩胶 2.95 － 1.25 0.8 0.001 0.1 5
一种抗肝素干扰的C反应蛋白检测试剂盒[发明专利]
![一种抗肝素干扰的C反应蛋白检测试剂盒[发明专利]](https://img.taocdn.com/s3/m/9be01b3a76a20029bc642d4c.png)
专利名称：一种抗肝素干扰的C反应蛋白检测试剂盒专利类型：发明专利
发明人：邵丹,赵民,王美丽
申请号：CN201811111054.X
申请日：20180922
公开号：CN109297919A
公开日：
20190201
专利内容由知识产权出版社提供
摘要：本发明涉及临床体外检测试剂技术领域，特别涉及一种抗肝素干扰的C反应蛋白检测试剂盒。
本发明试剂盒是双试剂液体型试剂盒，试剂R1包括乙二胺四乙酸二钠、磷酸氢二钠、磷酸二氢钾、氯化钠、聚乙二醇，试剂R2包括乙二胺四乙酸二钠、磷酸氢二钠、磷酸二氢钾、氯化钠、聚乙二醇、CRP抗体。
本发明试剂组分中聚乙二醇6000投料减少，可解决肝素干扰问题，具有很好的市场潜力。
申请人：山东博科生物产业有限公司
地址：250200 山东省济南市章丘区明水经济开发区(经十东路与明埠路交界山东博科产业园)国籍：CN
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SDSPAGE所有详细试剂配方
SDSPAGE所有详细试剂配方SDS-（聚丙烯酰胺凝胶电泳）是一种常用于蛋白质分析的实验技术。
该技术通常涉及到几种试剂，如胶溶液、缓冲液、电泳缓冲液、染色溶液等。
下面是SDS-常见的试剂配方及其详细说明。
一、胶溶液配方：1. 30%（w/v）丙烯酰胺（acrylamide）溶液- 在称量瓶中称取30g丙烯酰胺，并用蒸馏水调至100ml。
搅拌溶解直至均匀。
-这是制备聚丙烯酰胺凝胶的基础溶液。
2. 响应稀释液（Response diluent）-用于稀释聚丙烯酰胺溶液以获得不同浓度的凝胶。
-可通过将聚丙烯酰胺溶液与水按1:3体积比混合来制备响应稀释液。
二、缓冲液配方：1. 离子化追踪剂（Tris-Glycine running buffer）-用于凝胶电泳盒中电解质的运行。
-配方为：3g Tris base （pH 8.8）14.4g glycine将固体溶解于1L蒸馏水中，并调整pH至8.8三、电泳缓冲液配方：1. 离子化追踪剂（Tris-Glycine SDS running buffer）-用于在电泳过程中提供离子追踪剂。
-配方为：30g Tris base144g glycine10gSDS将固体溶解于1L蒸馏水中。
2. 加速剂（stacking gel buffer）- 用于形成凝胶中的聚集堆积层（stacking layer）。
-配方为：30g Tris base （pH 6.8）144g glycine1gSDS将固体溶解于1L蒸馏水中，并调整pH至6.8四、样品处理缓冲液配方：1. 样品加载缓冲液（sample loading buffer）-用于处理蛋白样品，以便在凝胶上获得良好的分离效果。
-配方为：0.5M Tris-HCl（pH 6.8）20%（v/v）甘油10%（w/v）SDS0.05%（w/v）溴酚蓝（bromophenol blue）旋转混合并加入蛋白样品。
五、凝胶染色溶液配方：1. 吸附性染色剂（Coomassie blue staining solution）-用于染色聚丙烯酰胺凝胶上分离的蛋白质。
生物化学实验：SDS-聚丙烯酰胺凝胶电泳(PAGE)
注意的问题
蛋白质电泳 常用的SDS-PAGE：单一亚基组成的蛋白质 非变性PAGE：多个不同亚基组成的蛋白质
聚丙烯酰胺具有神经毒性，操作时要戴手套
试剂
1.40%丙烯酰胺（Acr）：
2.10%SDS（十二烷基硫酸钠）
3.1.5mol/L pH8.8 Tris-HCl缓冲液：称取Tris18.2g ，加入50ml水，用1mol/L盐酸调pH8.8， 最后用 蒸馏水定容至100ml
（11）脱色液：冰乙酸75ml，甲醇50ml，加蒸馏水 定容至1000ml
（12）电极缓冲液（内含0.1%SDS，0.05mol/LTris0.384mol/L甘氨酸缓冲液pH8.3）：称Tris6.0g， 甘氨酸28.8g，加入SDS1g，加蒸馏水使其溶解后定 容至1000ml
Gel
5%
10%
200
200
97
97
66
66 45
29 45
29
15%
200 97 66
45
29
• Ammonium (AP) (N2H8S2O8; MW: 228.2). APS is a source of free radicals and is often used as an initiator for gel formation.
高, 样品下沉时易发生扩散，溢出加样孔
8.电泳槽中加入缓冲液，接通电源，进行电泳，溴酚蓝距 凝胶边缘约数cm时，停止电泳
9.凝胶板剥离与染色：电泳结束后，撬开玻璃板，将凝 胶板做好标记后放在大培养皿内，加入考马斯亮蓝染色染 色液，染色20-30min左右
10.脱色：染色后的凝胶板用蒸馏水漂洗数次，再用脱色 液脱色，直到蛋白质区带清晰 *剥胶时要小心,保持胶完好无损,染色要充分
SDS-聚丙烯酰胺凝胶电泳实验原理和操作步骤
实验原理：SDS-PAGE是对蛋白质进行量化，比较及特性鉴定的一种经济、快速、而且可重复的方法。
该法是依据混合蛋白的分子量不同来进行分离的。
SDS是一种去垢剂，可与蛋白质的疏水部分相结合，破坏其折叠结构，并使其广泛存在于一个广泛均一的溶液中。
SDS蛋白质复合物的长度与其分子量成正比。
在样品介质和凝胶中加入强还原剂和去污剂后，电荷因素可被忽略。
蛋白亚基的迁移率取决于亚基分子量。
试剂和器材：试剂：1. 5x样品缓冲液（10ml）: 1mol/L的Tris-HCl,5ml 50%甘油，2ml 10%的SDS，巯基乙醇，1ml 1%溴酚蓝，蒸馏水。
可在4℃保存数周，或在－20℃保存数月。
2. 凝胶贮液：在通风橱中，称取丙烯酰胺30g，甲叉双丙烯酰胺，加重蒸水溶解后，定容到100ml。
过滤后置棕色瓶中，4℃保存，一般可放置1个月。
3. 分离胶缓冲液： Tris ，加1mol/L HCl 48ml，加重蒸水80ml 使其溶解，调，定容至100ml，4℃保存。
4. 浓缩胶缓冲液： Tris ，加1mol/L HCl 48ml，加重蒸水80ml 使其溶解，调，定容至100ml，4℃保存。
5. TEMED（四乙基乙二胺）原液%过硫酸铵（用重蒸水新鲜配制）7. Tris-甘氨酸电极缓冲液：称取Tris ，甘氨酸，加蒸馏水约900ml，调后，用蒸馏水定容至1000ml。
置4℃保存，临用前稀释10倍。
8. 考马斯亮蓝G250染色液：称100mg考马斯亮蓝G250，溶于200ml蒸馏水中，慢慢加入 70%的过氯酸，最后补足水到250ml，搅拌1小时，小孔滤纸过滤。
器材：电泳仪，电泳槽，水浴锅，摇床。
实验操作（一）样品制备将蛋白质样品与5X样品缓冲液（20ul＋5ul）在一个Eppendorf 管中混合。
放入100℃加热 5－10min，取上清点样。
（二）分离胶及浓缩胶的制备1 将玻璃板、样品梳、Spacer用洗涤剂洗净，用ddH2O 冲洗数次，再用乙醇擦拭，晾干；2 将两块洗净的玻璃板之间加入Spacer，按照Bio-Rad Mini Ⅱ/Ⅲ说明书提示装好玻璃板；3 按如下体积配制10％分离胶 ml，混匀；ddH2O mlmol/LTris-HCl pH= ml30% Acr-Bis ml10% SDS 80 ul10%AP 56 ulTEMED 6 ul4 向玻璃板间灌制分离胶，立即覆一层重蒸水，大约20 min后胶即可聚合；5 按如下体积配制6％浓缩胶 ml，混匀；ddH2O mol/LTris-HCl pH= 30% Acr-Bisml 400 ul 600 ul10% SDS 10% AP TEMED36ul 24ul 4ul6 将上层重蒸水倾去，滤纸吸干，灌制浓缩胶，插入样品梳；7 装好电泳系统，加入电极缓冲液，上样20 μl;8 稳压200V，溴酚蓝刚跑出分离胶时，停止电泳，约需45 min～1hr.9 卸下胶板，剥离胶放入染色液中，室温染色1～2 hr；加入脱色液，置于80 rpm脱色摇床上,每20 min更换一次脱色液（10 ml冰乙酸；45 ml乙醇；45 ml蒸馏水）至完全脱净。
降纤酶SDS—聚丙烯酰胺凝胶电泳操作规程
降纤酶SDS—聚丙烯酰胺凝胶电泳操作规程目的：建立降纤酶SDS-聚丙烯酰胺凝胶电泳的操作规程，保证正确操作。
2. 依据：中华人民共和国卫生部标准（试行）（97）卫药标字01号。
3. 范围：适用于SDS-聚丙烯酰胺凝胶电泳测定降纤酶分子量及纯度的操作。
4. 职责：QC检验员对本标准的实施负责。
5. 程序：5.1. 定义：SDS-聚丙烯酰胺凝胶电泳测定蛋白分子量，是根据大多数蛋白都能与阴离子表面活性剂十二烷基硫酸钠（SDS）按重量比结合成复合物，使蛋白分子所带的负电荷远远超过天然蛋白分子的净电荷，消除了不同蛋白分子的电荷效应，使蛋白分子相对迁移率（Rf）的大小完全取决于分子量的高低，因此可从已知分子量的标准蛋白的对数和相对迁移率所作的标准曲线中求出供试品的分子量。
5.2. 仪器装置：由稳压电泳仪和垂直板电泳槽组成。
5.3. 试剂与仪器：β-巯基乙醇、丙烯酰胺、N，N ′—亚甲基双丙烯酰胺、三羟甲基氨基甲烷、十二烷基硫酸钠（SDS）、溴酚蓝、甘氨酸、过硫酸铵、三氯醋酸、甲醇、考马斯亮蓝R-250、甘油、盐酸、醋酸、四甲基乙二胺。
烧杯、刻度吸管、微量移液器。
5.4. 溶液的配制：5.4.1.丙烯酰胺—N，N′—亚甲基双丙烯酰胺贮备溶液：称取丙烯酰胺29.2g。
N、N′—亚甲基双丙烯酰胺0.8g，加水溶解，并稀释至100ml，过滤至棕色瓶中，4℃保存。
5.4.2.三羟甲基氨基甲烷缓冲液（pH8.8）：称取三羟甲基氨基甲烷18.15g，加水溶解，用2mol/L盐酸溶液调节pH值至8.8，加水稀释至100ml，4℃保存。
5.4.3.三羟甲基氨基甲烷缓冲液（pH6.8）：量取上述三羟甲基氨基甲烷缓冲液（pH8.8）适量，加水（1：3）稀释，用2mol/L盐酸液调节pH值至6.8，4℃保存。
5.4.4.10%十二烷基硫酸钠溶液：称取十二烷基硫酸钠10g，加水溶解，并稀释至100ml，室温保存。
5.4.5.供试品缓冲液：水 4.8ml三羟甲基氨基甲烷缓冲液(6.8) 1.2ml甘油 1.0ml10%SDS溶液 2.0ml0.5%(W/V)溴酚蓝溶液0.5mlβ-巯基乙醇0.5ml总体积10.0ml5.4.6.电极贮存液（pH8.3）：称取三羟甲基氨基甲烷15g，甘氨酸72g，SDS5g，加水溶解，并稀释至1000ml，4℃保存。
SDS-聚丙烯酰胺凝胶电泳
聚丙烯酰胺凝胶电泳PAGE作用：用于蛋白质与寡糖核苷酸的分离。
作用原理聚丙烯酰胺凝胶电泳是网状结构，具有分子筛效应，它具有两种形式，一种是非变性聚丙烯酰胺凝胶，蛋白质在电泳中保持完整的状态，蛋白在其中依三种因素分开：蛋白大小，形状和电荷。
而SDS-PAGE仅根据蛋白分子量亚基的不同而分离蛋白。
这个技术首先是196 7年由shapiro建立，他们发现在样品介质和丙烯酰胺凝胶中加入离子去污剂和强还原剂后，蛋白质亚基的电泳迁移率主要取决于亚基分子量的大小，电荷因素可以忽视。
作用：SDS是阴离子去污剂，作为变性剂和助溶试剂，它能断裂分子内和分子间的氢键，使分子去折叠，破坏蛋白分子的二、三级结构。
而强还原剂如巯基乙醇，二硫苏糖醇能使绊胱氨酸残基间的二硫键断裂。
在样品和凝胶中加入还原剂和SDS后，分子被解聚成多肽链，解聚后的氨基酸侧链和SDS结合成蛋白- SDS胶束，所带的负电荷大大超过了蛋白原有的电荷量，这样就消除了不同分子间的电荷差异和结构差异。
SDS-PAGE一般采用的是不连续缓冲系统，于连续缓冲系统相比，能够有较高的分辨率。
浓缩胶的作用是有堆积作用，凝胶浓度较小，孔径较大，把较稀的样品加在浓缩胶上，经过大孔径凝胶的迁移作用而被浓缩至一个狭窄的区带。
当样品液和浓缩胶选TRIS/HCL缓冲液，电极液选TRIS/甘氨酸。
电泳开始后，HCL解离成氯离子，甘氨酸解离出少量的甘氨酸根离子。
蛋白质带负电荷，因此一起向正极移动，其中氯离子最快，甘氨酸根离子最慢，蛋白居中。
电泳开始时氯离子泳动率最大，超过蛋白，因此在后面形成低电导区，而电场强度与低电导区成反比，因而产生较高的电场强度，使蛋白和甘氨酸根离子迅速移动，形成以稳定的界面，使蛋白聚集在移动界面附近，浓缩成一中间层。
此鉴定方法中，蛋白质的迁移率主要取决于它的相对分子质量，而与所带电荷和分子形状无关。
补充信息聚丙烯酰胺凝胶电泳简称为PAGE，是以聚丙烯酰胺凝胶作为支持介质的一种常用电泳技术。
SDS聚丙烯凝胶电泳实验方案
SDS聚丙烯凝胶电泳一、试剂（1）分离胶缓冲液（Tris-HCl缓冲液PH8.9）:取1mol/L盐酸48mL，Tris 36.3g,用无离子水溶解后定容至100mL。
（2）浓缩胶缓冲液（Tris-HCl缓冲液PH6.7）:取1mol/L盐酸48mL, Tris 5.98g,用无离子水溶解后定容至100mL。
（3）30%分离胶贮液：配制方法与连续体系相同，称丙烯酰胺（Acr）30g及N，N’-甲叉双丙烯酰胺（Bis）0.8g，溶于重蒸水中，最后定容至100ml，过滤后置棕色试剂瓶中，4℃保存。
（4）10%SDS溶液：SDS在低温易析出结晶，用前微热，使其完全溶解。
（5）1%TEMED；（6）10%过硫酸铵（AP）：现用现配。
（7）电泳缓冲液（Tris-甘氨酸缓冲液PH8.3）:称取Tris 6.0g, 甘氨酸28.8g, SDS1.0g, 用无离子水溶解后定容至1L。
（8）样品溶解液：取SDS 100mg，巯基乙醇0.1mL，甘油1mL，溴酚蓝2mg，0.2mol/L，pH7.2磷酸缓冲液0.5mL，加重蒸水至10mL（遇液体样品浓度增加一倍配制）。
用来溶解标准蛋白质及待测固体。
（9）染色液：0.25g考马斯亮蓝G-250，加入454mL 50%甲醇溶液和46mL冰乙酸即可。
（10）脱色液：75mL冰乙酸，875mL重蒸水与50mL甲醇混匀。
二.实验步骤1、组装模具1）用海绵或纱布沾取少量清洁剂（也可用纯酒精浸泡），擦洗干净两块玻璃板，自来水冲洗3-5遍，然后，再用无水乙醇冲洗2遍，晾干待用。
2）将已冲洗好的玻璃板心均匀涂上凡士林，将玻璃板心放在玻璃板的两侧（下面一定要靠边），再将另一块玻璃板盖上待用。
3）将隔条安置好，再将事先准备好的琼脂溶液倒入电泳仪的槽中，再将两块玻璃板插入槽中，有缺口的放在里侧，用夹子夹好待用。
2、制胶1）20ML10%分离胶/ML分离胶贮液 6.66分离胶缓冲液（PH8.9） 2.510%SDS 0.21%TEMET 2.00重蒸水8.5410%AP 0.1（AP最后加入，注意观察是否漏胶）2）水封，等待20-30分钟，用滤纸吸干3）10ML 3%浓缩胶/ML分离胶贮液 3.0浓缩胶缓冲液（6.8） 1.2510%SDS 0.11%TEMED 2.00重蒸水 4.610%AP 0.14)插上齿梳，确保无气泡。
丙烯酰胺苯硼酸 分子印迹
丙烯酰胺苯硼酸分子印迹
丙烯酰胺苯硼酸是一种有机化合物，它在许多应用中都有潜在的价值，特别是在分子印迹技术中。
1. 分子印迹技术(Molecular Imprinting Technology， MIT):这是一种合成技术，其中模板分子与功能单体和交联剂反应，形成具有特定三维结构的“印迹”分子。
这些印迹分子能够与特定的目标分子(称为“模板”或“被分析物”)高度选择性地结合。
2. 丙烯酰胺苯硼酸的应用:由于其独特的化学结构和性质，丙烯酰胺苯硼酸经常被用作分子印迹聚合物(MIPs)的交联剂或功能单体。
这使得MIPs能够与特定的目标分子结合，从而实现高选择性的分离、纯化或检测。
3. 优势:与其他传统的分离技术相比，分子印迹技术具有更高的选择性和亲和力，因为它是基于目标分子与模板分子之间的特异性相互作用。
4. 应用领域:分子印迹技术在化学、生物化学、药物分析、环境分析等领域都有广泛的应用。
例如，它可以用于检测和分离有毒或有害物质、研究生物大分子的结构和功能、以及开发新的药物传递系统等。
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Inhibitors, Agonists, Screening LibrariesSafety Data Sheet Revision Date:May-24-2017Print Date:May-24-20171. PRODUCT AND COMPANY IDENTIFICATION1.1 Product identifierProduct name :PP58Catalog No. :HY-18622CAS No. :212391-58-71.2 Relevant identified uses of the substance or mixture and uses advised againstIdentified uses :Laboratory chemicals, manufacture of substances.1.3 Details of the supplier of the safety data sheetCompany:MedChemExpress USATel:609-228-6898Fax:609-228-5909E-mail:sales@1.4 Emergency telephone numberEmergency Phone #:609-228-68982. HAZARDS IDENTIFICATION2.1 Classification of the substance or mixtureNot a hazardous substance or mixture.2.2 GHS Label elements, including precautionary statementsNot a hazardous substance or mixture.2.3 Other hazardsNone.3. COMPOSITION/INFORMATION ON INGREDIENTS3.1 SubstancesSynonyms:PP⁻58; PP 58Formula:C22H19Cl2N5O2Molecular Weight:456.32CAS No. :212391-58-74. FIRST AID MEASURES4.1 Description of first aid measuresEye contactRemove any contact lenses, locate eye-wash station, and flush eyes immediately with large amounts of water. Separate eyelids with fingers to ensure adequate flushing. Promptly call a physician.Skin contactRinse skin thoroughly with large amounts of water. Remove contaminated clothing and shoes and call a physician.InhalationImmediately relocate self or casualty to fresh air. If breathing is difficult, give cardiopulmonary resuscitation (CPR). Avoid mouth-to-mouth resuscitation.IngestionWash out mouth with water; Do NOT induce vomiting; call a physician.4.2 Most important symptoms and effects, both acute and delayedThe most important known symptoms and effects are described in the labelling (see section 2.2).4.3 Indication of any immediate medical attention and special treatment neededTreat symptomatically.5. FIRE FIGHTING MEASURES5.1 Extinguishing mediaSuitable extinguishing mediaUse water spray, dry chemical, foam, and carbon dioxide fire extinguisher.5.2 Special hazards arising from the substance or mixtureDuring combustion, may emit irritant fumes.5.3 Advice for firefightersWear self-contained breathing apparatus and protective clothing.6. ACCIDENTAL RELEASE MEASURES6.1 Personal precautions, protective equipment and emergency proceduresUse full personal protective equipment. Avoid breathing vapors, mist, dust or gas. Ensure adequate ventilation. Evacuate personnel to safe areas.Refer to protective measures listed in sections 8.6.2 Environmental precautionsTry to prevent further leakage or spillage. Keep the product away from drains or water courses.6.3 Methods and materials for containment and cleaning upAbsorb solutions with finely-powdered liquid-binding material (diatomite, universal binders); Decontaminate surfaces and equipment by scrubbing with alcohol; Dispose of contaminated material according to Section 13.7. HANDLING AND STORAGE7.1 Precautions for safe handlingAvoid inhalation, contact with eyes and skin. Avoid dust and aerosol formation. Use only in areas with appropriate exhaust ventilation.7.2 Conditions for safe storage, including any incompatibilitiesKeep container tightly sealed in cool, well-ventilated area. Keep away from direct sunlight and sources of ignition.Recommended storage temperature:Powder-20°C 3 years4°C 2 yearsIn solvent-80°C 6 months-20°C 1 monthShipping at room temperature if less than 2 weeks.7.3 Specific end use(s)No data available.8. EXPOSURE CONTROLS/PERSONAL PROTECTION8.1 Control parametersComponents with workplace control parametersThis product contains no substances with occupational exposure limit values.8.2 Exposure controlsEngineering controlsEnsure adequate ventilation. Provide accessible safety shower and eye wash station.Personal protective equipmentEye protection Safety goggles with side-shields.Hand protection Protective gloves.Skin and body protection Impervious clothing.Respiratory protection Suitable respirator.Environmental exposure controls Keep the product away from drains, water courses or the soil. Cleanspillages in a safe way as soon as possible.9. PHYSICAL AND CHEMICAL PROPERTIES9.1 Information on basic physical and chemical propertiesAppearance Light yellow to yellow (Solid)Odor No data availableOdor threshold No data availablepH No data availableMelting/freezing point No data availableBoiling point/range No data availableFlash point No data availableEvaporation rate No data availableFlammability (solid, gas)No data availableUpper/lower flammability or explosive limits No data availableVapor pressure No data availableVapor density No data availableRelative density No data availableWater Solubility No data availablePartition coefficient No data availableAuto-ignition temperature No data availableDecomposition temperature No data availableViscosity No data availableExplosive properties No data availableOxidizing properties No data available9.2 Other safety informationNo data available.10. STABILITY AND REACTIVITY10.1 ReactivityNo data available.10.2 Chemical stabilityStable under recommended storage conditions.10.3 Possibility of hazardous reactionsNo data available.10.4 Conditions to avoidNo data available.10.5 Incompatible materialsStrong acids/alkalis, strong oxidising/reducing agents.10.6 Hazardous decomposition productsUnder fire conditions, may decompose and emit toxic fumes.Other decomposition products - no data available.11.TOXICOLOGICAL INFORMATION11.1 Information on toxicological effectsAcute toxicityClassified based on available data. For more details, see section 2Skin corrosion/irritationClassified based on available data. For more details, see section 2Serious eye damage/irritationClassified based on available data. For more details, see section 2Respiratory or skin sensitizationClassified based on available data. For more details, see section 2Germ cell mutagenicityClassified based on available data. For more details, see section 2CarcinogenicityIARC: No component of this product present at a level equal to or greater than 0.1% is identified as probable, possible or confirmed human carcinogen by IARC.ACGIH: No component of this product present at a level equal to or greater than 0.1% is identified as a potential or confirmed carcinogen by ACGIH.NTP: No component of this product present at a level equal to or greater than 0.1% is identified as a anticipated or confirmed carcinogen by NTP.OSHA: No component of this product present at a level equal to or greater than 0.1% is identified as a potential or confirmed carcinogen by OSHA.Reproductive toxicityClassified based on available data. For more details, see section 2Specific target organ toxicity - single exposureClassified based on available data. For more details, see section 2Specific target organ toxicity - repeated exposureClassified based on available data. For more details, see section 2Aspiration hazardClassified based on available data. For more details, see section 212. ECOLOGICAL INFORMATION12.1 ToxicityNo data available.12.2 Persistence and degradabilityNo data available.12.3 Bioaccumlative potentialNo data available.12.4 Mobility in soilNo data available.12.5 Results of PBT and vPvB assessmentPBT/vPvB assessment unavailable as chemical safety assessment not required or not conducted.12.6 Other adverse effectsNo data available.13. DISPOSAL CONSIDERATIONS13.1 Waste treatment methodsProductDispose substance in accordance with prevailing country, federal, state and local regulations.Contaminated packagingConduct recycling or disposal in accordance with prevailing country, federal, state and local regulations.14. TRANSPORT INFORMATIONDOT (US)This substance is considered to be non-hazardous for transport.IMDGThis substance is considered to be non-hazardous for transport.IATAThis substance is considered to be non-hazardous for transport.15. REGULATORY INFORMATIONSARA 302 Components:No chemicals in this material are subject to the reporting requirements of SARA Title III, Section 302.SARA 313 Components:This material does not contain any chemical components with known CAS numbers that exceed the threshold (De Minimis) reporting levels established by SARA Title III, Section 313.SARA 311/312 Hazards:No SARA Hazards.Massachusetts Right To Know Components:No components are subject to the Massachusetts Right to Know Act.Pennsylvania Right To Know Components:No components are subject to the Pennsylvania Right to Know Act.New Jersey Right To Know Components:No components are subject to the New Jersey Right to Know Act.California Prop. 65 Components:This product does not contain any chemicals known to State of California to cause cancer, birth defects, or anyother reproductive harm.16. OTHER INFORMATIONCopyright 2017 MedChemExpress. The above information is correct to the best of our present knowledge but does not purport to be all inclusive and should be used only as a guide. The product is for research use only and for experienced personnel. It must only be handled by suitably qualified experienced scientists in appropriately equipped and authorized facilities. The burden of safe use of this material rests entirely with the user. MedChemExpress disclaims all liability for any damage resulting from handling or from contact with this product.Caution: Product has not been fully validated for medical applications. For research use only.Tel: 609-228-6898 Fax: 609-228-5909 E-mail: tech@Address: 1 Deer Park Dr, Suite Q, Monmouth Junction, NJ 08852, USA。