pTRE2-hygro哺乳动物表达载体说明
真核细胞常见的表达载体及真核细胞表达外源基因的调控(精)
真核细胞常见表达载体1. pCMVp-NEO-BAN载体特点: 该真核细胞表达载体分子量为6600碱基对，主要由CMVp启动子、兔β-球蛋白基因内含子、聚腺嘌呤、氨青霉素抗性基因和抗neo基因以及pBR322骨架构成，在大多数真核细胞内都能高水平稳定地表达外源目的基因。
更重要的是，由于该真核细胞表达载体中抗neo基因存在，转染细胞后，用G418筛选，可建立稳定的、高表达目的基因的细胞株。
插入外源基因的克隆位点包括Sal1、BamH1和EcoR1位点。
注意在此载体中有二个EcoR1位点存在。
2. pEGFP, 增强型绦色荧光蛋白表达载体(Enhanced Fluorecent Protein Vector特点: pEGFP表达载体中含有绿色荧光蛋白，在PCMV启动子驱动下，在真核细胞中高水平表达。
载体骨架中的SV40 origin使该载体在任何表达SV40 T 抗原的真核细胞内进行复制。
Neo抗性盒由SV40早期启动子、Tn5的neomycin/kanamycin抗性基因以及HSV-TK基因的聚腺嘌呤信号组成，能应用G418筛选稳定转染的真核细胞株。
此外，载体中的pUC origin 能保证该载体在大肠杆菌中的复制，而位于此表达盒上游的细菌启动子能驱动kanamycin抗性基因在大肠杆菌中的表达。
用途: 该表达载体EGFP上游有Nde1、Eco47111和Age1克隆位点，将外源基因扦入这些位点，将合成外源基因和EGFP的融合基因。
借此可确定外源基因在细胞内的表达和/或组织中的定位。
亦可用于检测克隆的启动子活性(取代CMV启动子,Acet1-Nhe1。
Excitation maximum = 488 nm; Emission maximum = 507图示为启动子分泌信号肽和多克隆位点区域：Ase1.pCMV…ccg cta gcg cta ccg gtc gcc acc atg- .EGFP…BamH1…SV40 poly A+Nhe1 Age13. pEGFT-Actin, 增强型绿色荧光蛋白/人肌动蛋白表达载体特点: pEGFP-Actin表达载体中含有绿色荧光蛋白和人胞浆β-肌动蛋白基因，在PCMV启动子驱动下，在真核细胞中高水平表达。
pFR-luc使用说明
pFR-luc编号载体名称北京华越洋VECT76052pFR-lucpFR-luc载体相关的哺乳动物表达载体：SuperCos I pDsRed2-Bid pNFκB-MetLuc2-ReporterpYr-adshuttle-3pAcGFP1-N1pEF1α-IRES-DsRed-Express2pVitro2-neo-mcs pSecTag2A pCMV-DsRed-Express2pUB6/V5-His/LacZ pGL4.27pcDNA3.1/NT-GFP-TOPOpUB6/V5-His C pGL4.26pEF1α-IRES-ZsGreen1pUB6/V5-His B pACT pCMV-Tag2ApUB6/V5-His A pBIND-Id Control pCMV-Tag5BpTracer-CMV2pTRE2pAcGFP1-C In-Fusion ReadypSV-β-Galactosidase pRevTRE p3XFLAG-CMV-14pSI pTK-hyg p3XFLAG-CMV-8pSG5pTRE3G-Luc pFLAG-CMV-2pSFV1pSwitch pcDNA3.3-TOPOpSecTag2/Hygro A pcDNA4/His C pcDNA6.2/cLumio-DESTpSecTag B c-Flag pcDNA3pCMV-tdTomatopRluc-N2pcDNA4/TO/Myc-His A pAcGFP1-MitopPICZalpha D pcDNA6/myc-His B pAcGFP1-N In-Fusion Ready pORF-lacZ pcDNA6/V5-His B pDsRed-Monomer-N In-Fusion Ready pORF-HSV1tk pcDNA6.2/nTC-Tag-DEST pcDNA4/TO/Myc-His BpOG44pOptiVEC-TOPO pIRES2-EGFPpNTAP-B pcDNA5/FRT pcDNA3.1/His CpMEP4pGL4.30pcDNA3.1/CT-GFP-TOPOpLVX-ZsGreen-miRNA-Puro pGL4.19pEF1α-IRES-AcGFP1pLVX-IRES-Puro-3xFlag pACT-MyoD pcDNA3.2/V5/GW/D-TOPO pLPCX pCMV-BD pcDNA4/TO/Myc-His/LacZ pLEGFP-N1pCMV-Tet3G pcDNA4/HisMax-TOPOpKH3pTet on advanced p3XFLAG-CMV-13pIRES-puro2pTRE-Tight p3xFLAG-CMV-10phRL-TK pIND pFLAG-CMV-3pG5lac pGene/V5-His B pcDNA4/TO/Myc-His CpFR-luc pOPRSVI pcDNA6.2/C-YFP-DESTpEF6/myc-His C pcDNA4/HisMax A pcDNA6.2/cTC-Tag-DESTpEF4/V5-His A pIRESpuro3pcDNA6.2/nGeneBLAzer-DESTpEF1/myc-His lacZ pIRESneo3pCRE-MetLuc2-ReporterpEF1/myc-His C pIRESneo2pEF1α-DsRed-Express2pEF1/myc-His B pcDNA4/myc-His A pDsRed-Express-C1pEF1/myc-His A pCMV-PKA pEF1α-DsRed-Monomer-N1 pECFP-Mito pAcGFP1-N3pDD-AmCyan1ReporterpECFP-ER pcDNA5/FRT/TO pCRE-DD-AmCyan1pDP8rs pBApo-CMV-Pur pIRES2-DsRed-Express2pDP5rs pBApo-EF1α-pur pDsRed-Express-N1pDP4rs ptdTomato-N1pcDNA6.2/nLumio-DESTpDP3rs pAcGFP1-Golgi pcDNA6/myc-His ApDP2rs pAcGFP1-p53pcDNA6/V5-His ApDP1rs pAcGFP1-Actin pcDNA5/FRT/TO-TOPOpCMV-Myc-C pBI-CMV2pcDNA6.2/V5/GW/D-TOPOpCMV-HA-N pEF1α-tdTomato pcDNA6.2/cGeneBLAzer-DEST pCMV-HA-C pEF1α-tdTomato pcDNA6.2/nGeneBLAzer-GW/D-TOPO pCMV6-XL4pEBVHis A pcDNA5/TOpCMV6-AC-GFP pGL4.10pBApo-CMV-neopCMV5pGL4.29pDsRed-MonomerpCI pGL4.13pIRESpCGN pG5luciferase pIRES-hrGFP-1apcDNA6.2/EmGFP-Bsd/V5-DEST pCMV-AD pDsRed-Express2-N1pcDNA4/V5-His A pRevTet-Off pCMV-Tag3BpcDNA3-mRFP pTet-Off pCRE-hrGFPpcDNA3-CFP pTRE2-hygro pDsRED2-MitopcDNA3.1(+)/myc-His C pVgRxR pAcGFP1-FpcDNA3.1(+)/myc-His A pOPI3CAT pAcGFP1-C1pcDNA3.1(+)/CAT pBK-RSV pAsRed2-C1pcDNA3.1(-)/myc-His C pIRES2-DsRed2pAsRed2-N1pcDNA3.1/Zeo(+)pCMV-Myc pAcGFP1-LampcDNA3.1/Zeo(-)pCMV-Tag2C pAcGFP1-CpcDNA3.1/V5-His C pCMV-Tag5A pSEAP2-BasicpcDNA3.1/Hygro(+)pCMV-Tag3C pBI-CMV3pcDNA3.1/Hygro(-)p3XFLAG-CMV-7pNFkB-DD-tdTomato pcDNA3.1/GS p3XFLAG-CMV-9pcDNA3.1/His A pBS185CMV-Cre pFLAG-CMV-4pEBVHis B pBIND-GFP pBI-CMV4pGL4.75pBiFC-VN155(I152L)pcDNA4/His A pGL4.20pBiFC-CC155pcDNA4/myc-His B pCMV-SPORT6 pBiFC-CrN173pcDNA4/HisMax C pCMV-SPORT6 pBiFC-bJunVN155(I152L)pCMV-Tag4A pBINDpBD-p53pcDNA6/myc-His C pBD-NF-κBpBD-NF-kB pCMV-Tag2B pRevTet-OnpBC1pGRN145pTet-OnpAD-TRAF pCMV-MEK1pTRE3GpAD-SV40T pCMV-Tag3A pcDNA4/TO pAAV-ZsGreen-miRNA pCMVLacI pcDNA4/His B pAAV-tTS-shRNA pBI-CMV1pcDNA4/HisMax B pIREShyg3pEF1α-AcGFP1-N1pcDNA4/myc-His C pcDNA6/TR pCMV-LacZ pcDNA3.1/His B pDsRed-Express2-C1pCMV-Tag4B pcDNA6/V5-His C pBI-CMV5pCMV-Tag5C pCHO1.0 pAmCyan1-C1plRES2-ZsGreen1pGL3-Promoter pAcGFP1-Mem p3XFLAG-CMV-7.1pCMV-MEKK1 pAcGFP1-C3pFLAG-CMV-5a pFLAG-CMV2pTT5pBudCE4.1pAcGFP1-C2 pSEAP2-Control pREP4ptdTomato-C1 pIRES2-AcGFP1pBApo-CMV pCRE-DD-tdTomato pAcGFP1-Hyg-C1。
pCDH-CMV-MCS-EF1-Hygro使用说明
pCDH-CMV-MCS-EF1-HygropCDH-CMV-MCS-EF1-Hygro 载体基本信息：载体名称:pCDH-CMV-MCS-EF1-Hygro 质粒类型: 慢病毒表达载体；cDNA 表达载体；双启动子载体 克隆方法: 多克隆位点，限制性内切酶启动子:CMV 载体大小:-- 5' 测序引物及序列:CMV-F :CGCAAATGGGCGGTAGGCGTG 3' 测序引物及序列:-- 载体标签: 无 载体抗性: 氨苄青霉素（Ampicillin ）筛选标记:Hygromycin 克隆菌株: E.coli cells(RecA-)推荐: Stbl2 ,OmniMAX 2 T1R 宿主细胞（系）: 常用细胞系，如HeLa, HEK293, HT1080, H1299备注: pCDH-CMV-MCS-EF1-Hygro 慢病毒表达载体是基于HIV 的慢病毒载体；用于cDNA 表达和克隆；高效转染细胞，建立稳定细胞系；CMV 启动子驱动目的基因的高水平表达，EF1a 启动子驱动报告基因的中等水平 的表达。
稳定性: 稳表达 组成型/诱导型: 组成型 病毒/非病毒: 慢病毒（HIV)pCDH-CMV-MCS-EF1-Hygro 载体质粒图谱和多克隆位点信息：pCDH-CMV-MCS-EF1-Hygro载体简介：背景简介：This manual provides details and information necessary to generate expression constructs of your gene of interest in the pCDH cDNA Cloning and Expression Lentivectors. Specifically, it provides critical instructions on amplification and cloning cDNA into the pCDH vectors, and verification of the final expression constructs. This manual does not include information on packaging the pCDH expression constructs into pseudotyped viral particles or transducing your target cells of choice with these particles. This information is available in the user manual Lentivector Expression Systems: Guide to Packaging and Transduction of Target Cells which is available on the SBI website. Before using the reagents and material supplied with this system, please read the entire manual.基于HIV-1的pCDH 慢病毒载体特征：Multiple Cloning Site (MCS)—for cloning the gene of interest in the MCS located downstream of the CMV promoter.WPRE element—enhances stability and translation of the CMV-driven transcripts.SV40 polyadenylation signal—enables efficient termination of transcription and processingof recombinant transcripts.Hybrid RSV/5LTR promoter—provides a high level of expression of the full-length viral transcript in producer 293 cells.Genetic elements (cPPT, gag, env, LTRs)—necessary for packaging, transducing, and stably integrating the vira expression construct into genomic DNA.SV40 origin—for stable propagation of the pCDH plasmid in mammalian cells.pUC origin—for high copy replication and maintenance of the plasmid in E.coli cells. Ampicillin resistance gene—for selection in E.coli cells.pCDH 慢病毒表达载体的优势：Lentiviral expression vectors are the most effective vehicles for the delivery and expression of a gene of interest to almost any mammalian cell—including non-dividing cells and model organisms (C.A. Machida, 2003; M. Federico, 2003; W. C. Heiser, 2004). As with standard plasmid vectors, it is possible to introduce lentivector expression constructs in plasmid form into the cells with low-to-medium efficiency using conventional transfection protocols. However, by packaging the lentivector construct into viral particles, you can obtain highly efficient transduction of expression constructs—even with the most difficult to transfect cells, such as primary, stem, and differentiated cells. The expression construct transduced in target cells is integrated into genomic DNA and provides stable, long-term expression of the target gene.pCDH 慢病毒载体的包装载体及细胞系The expression lentivector contains the genetic elements responsible for packaging, transduction, stable integration of the viral expression construct into genomic DNA, and expression of the target gene sequence. The packaging vector provides all the proteins essential for transcription and packaging of an RNA copy of the expression construct into recombinant viral particles. To produce a high titer of viral particles, expression and packaging vectors are transiently co-transfected into producer mammalian cells (e.g., HEK 293 cells). For a detailed description of SBI’s Lentivector expression system,please refer to the Lentivector Expression System user manual.启动子的选择：SBI provides a collection of cDNA cloning and expression vectors for various applications. A gene of interest can be cloned under a CMV or EF1 promoter with or without another expression cassette for a reporter gene (copGFP or PuroR). Genes can be either expressed transiently through transfection or stably expressed in a target cell line through transduction with packaged viral particles.The major concern of cDNA expression in lentivectors is the efficiency level and stability of expression in target cell lines.The Cytomegalovirus (CMV) promoter is a strong and most commonly used viral promoter that constitutively expresses downstream genes. While the CMV promoter works perfectly in the most common cell lines, it shows poor expression in some stem cell lines and hematopoietic cell lines (R.F. Doll, 1996; E.D. Papadakis, 2004).The housekeeping elongation factor 1α (EF1) promoter has been shown to exceed and outlast CMV-mediated expression in retroviral, lentiviral, and adenoviral vectors, in hematopoietic cell lines (K. Tokushige 1997; H. Nakai, 1998; C. Teschendorf, 2002). EF1 also performs well in most common cell lines.MSCV promoter is the 5’-LTR promoter of murine stem cell virus. When a portion of the U3 region of the 3’ HIV LTR was replaced with the U3 region of MSCV LTR, the resulted hybrid HIV/MSCV LTR has dramatically increased the transgene expression level in human CD34+ hematopoietic cells (J.K. Choi, 2001). After integration into genomic DNA, this promoter transcribes a long transcript with an intron in the 5’UTR flanked with splice donor and acceptor sites derived from the lentiviral vector. Further studies found that additional CpG mutations in the MSCV LTR reduced transcriptional silencing in embryonic stem cells (C.S. Swindle, 2004). We constructed cDNA expression vectors with the CpG-deficient MSCV incorporated into the 3’ HIV LTR. After integration into genomic DNA, 3’MSCV/LTR will replace the 5’LTR and provide a high level of expression of the target gene and reporter gene downstream.SBI第三代慢病毒载体SBI offers a third generation of the most popular HIV-1 based lentivector expression system which consists of three maincomponents:(1) The lentiviral expression vector (e.g., pCDH-EF1-MCS-T2A-Puro)(2) The lentiviral packaging plasmids (e.g., pPACKH1 Packaging Plasmid mix)(3) A pseudoviral particle producer cell line (e.g., 293TN cells)2A Peptide-enabled dual expression systemCoexpression of a reporter gene together with a gene of interest is a useful approach for selecting transfected or transduced cells. This is commonly achieved by using two independent internal promoters, such as CMV and EF1 in pCDH-CMV-MCSEF1- copGFP, or by linking two transgenes with an internal ribosomal entry site (IRES) element in a single bicistronic transcript. Many dual promoter pairs have shown a high level of expression of both transgenes in standard cell lines— however, promoter interference often occurs in some cell lines. There are also two main problems that limit the use of IRES: the large size and the imbalanced expression between the first and second cistrons (H. Mizuguchi, 2000; X.Yu, 2003).The “self-cleaving” 2A peptides have been used successfully to generate multiple proteins from a single promoter in many applications (P. de Felipe, 2004; M.J. Osborn, 2005; P. de Felipe, 2006). The 2A-like sequences exist in several viruses and are used to mediate protein cleavage from a single open reading frame. Through a ribosomal skip mechanism, the 2A peptide prevents normal peptide bond formation between the 2A glycine and the 2B proline without affecting the translation of 2B (M.L. Donnelly, 2001):SBI’s cDNA expression vectors incorporate the 2A-like sequence (T2A) from the insect virus Thosea asigna to mediate the coexpression of a reporter gene with the target cDNA. Reporter genes have been cloned at either the first or second positions, and we achieved high expression levels at both locations.pCDH-CMV-MCS-EF1-Hygro载体序列pCDH-CMV-MCS-EF1-Hygro其他相关慢病毒载体：Tet-pLKO-neo Tet-pLKO-puro pPACKH1-GAGpMD2.G pCMV-dR8.2-dvpr pLKO.1-GFP-shRNA pLKO.1-TRC control pLKO.1-hygro pLKO.1-TRCpCDH-MSCV-MCS-EF1-copGFP pCDH-MSCV-MCS-EF1-copGFP-T2A-Puro FUW-tetO-hOKMSFUW-tetO-hOCT4 FUW-tetO-hSOX2 FUW-tetO-hKLF4FUW pLVX-AcGFP1-N1 pLVX-AcGFP1-C1pLVX-AmCyan1-N1 pLVX-DsRed-Express2-C1 pLVX-DsRed-Express2-N1 pLVX-DsRed-Monomer-N1 pLVX-PAmCherry-C1 pLVX-PAmCherry-N1pLVX-ZsGreen1-N1 pLVX-IRES-ZsGreen1 pLVX-IRES-mCherrypLVX-mCherry-C1 pLVX-mCherry-N1 pLVX-tdTomato-C1pLKO.1-puro pLentilox 3.7 pLVX-Tet-On-Advanced pLVX-IRES-Puro pLVX-IRES-Neo pLVX-IRES-HygpLVX-EF1α-DsRed-Monomer-C1 pLVX-EF1α-AcGFP1-N1 pLVX-EF1α-AcGFP1-C1 pLVX-EF1α-mCherry-C1 pLVX-EF1α-IRES-mCherry pLVX-EF1α-IRES-ZsGreen1 pLVX-MetLuc Control pLVX-MetLuc pLVX-Hom-Mem1pLVX-Het-2 pLVX-DD-AcGFP1-Actin pPRIME-TET-GFP-FF3pSIH1-H1-CopGFP pCDH-EF1-MCS-T2A-Puro pCDH-CMV-MCS-EF1-Puro pCDF1-MCS2-EF1-copGFP pLOX-CWBmi1 pLOX-CW-CREpRSV-rev pMDLg-pRRE pLL3.7pLVX-DD-AmCyan1 Control pLVX-DD-AmCyan1 Reporter pLVX-DD-tdTomato Reporter pLVX-DD-tdTomato Control pLVX-PTuner-Green pLVX-CherryPicker2pLVX-TetOne-Puro-Luc pLVX-TetOne pLVX-TetOne-PuropLVX-TetOne-Luc pLVX-rHom-Nuc1 pLVX-rHom-Sec1pLVX-rHom-1 pLVX-Hom-Nuc1 pLVX-Het-Nuc1pLVX-PTuner pLVX-PTuner2 pLVX-DD-ZsGreen1 Reporter pLVX-Het-1 pLVX-CherryPicker Control pLVX-Tet3GpCDH-CMV-MCS-EF1-RFP-T2A-Puro pCDH-CMV-MCS-EF1-Hygro pCDH-CMV-MCS-EF1-Neo pCDH-MCS-T2A-Puro-MSCV pCDH1-MCS2-EF1-copGFP pCDF1-MCS2-EF1-Puro pCDH-EF1-MCS-T2A-copGFP pWPXL pLVX-TRE3G-ZsGreen1 pLVX-TRE3G-mCherry pLenti6.3-EmGFP-BveI miR pLenti6/V5-GW/lacZpLenti6.3/V5-GW/EmGFP pLenti6.3-MCS pLenti6.3-DsRed2-BveI miR pLenti6.3-MCS-IRES2-EGFP pLVX-shRNA2 psPAX2VSV-G pSico PGK Puro pcDNA6.2-DsRed2-MCS1 miR pcDNA6.3-EmGFP-NC- II pcDNA6.2-EmGFP-NC- I pcDNA6.2-EmGFP-BsaI miR pLenti6.3-BveI miR pLenti6.3-MCS-IRES2-DsRed2 pLEX-MCSpGIPZ pLP2 pLP1FUGW pFUGW pLOX-Ttag-iresTKpMDLg/pRRE pLentG-KOSM pCMV-dR8.91pLVX-TRE3G-Luc Control pLVX-TRE3G-IRES pCgpvpSico pSicoR pLVTHMpGensil-1 pLVX-EF1α-IRES-Puro pCDF1-MCS2-EF1-copGFP pPACKH1-REV pLVX-Het-Mem1 pLVX-shRNA1pLKO.1-puro-GFP-siRNA pPRIME-TREX-GFP-FF3 pcDNA6.2-DsRed2-BsmBI miR pCDH-MSCV-MCS-EF1-Puro pCDH-CMV-MCS-EF1-copGFP pLVX-TRE3GFUW-tetO-hMYC pLOX-TERT-iresTK pLP/VSVGFUW-M2rtTA pCDH-EF1-MCS-(PGK-Puro) pcDNA6.2-EmGFP-MCS1 miR pLVX-AmCyan1-C1 pLVX-Hom-1 pcDNA6.2-BsaI miRpLVX-DsRed-Monomer-C1 pLVX-mCherry-Actin pTRIPZpLVX-ZsGreen1-C1 pLVX-CherryPicker1 LeGO-iC2pLVX-IRES-tdTomato pCDH-CMV-MCS-EF1-copGFP-T2A-Puro pLKO.3GpLVX-tdTomato-N1 pLVX-PTuner2-C pLVX-PuropLVX-Tight-Puro pLVX-DD-ZsGreen1 Control pSicoR PGK PuropLVX-EF1α-DsRed-Monomer-N1 pCDH-UbC-MCS-EF1-Hygro pLVTHpLVX-EF1α-mCherry-N1 pCDH-CMV-MCS-EF1-RFP。
pAD-SV40T使用说明
pAD-SV40T编号载体名称北京华越洋VECT76070pAD-SV40TpAD-SV40T载体图谱:pAD-SV40T载体简介：The pAD-SV40T control plasmid expresses a hybrid protein which contains the NF-κB transcription activation domain fused to amino acids84–708of the SV40large T-antigen.5pAD-SV40T载体相关的哺乳动物表达载体：SuperCos I pDsRed2-Bid pNFκB-MetLuc2-ReporterpYr-adshuttle-3pAcGFP1-N1pEF1α-IRES-DsRed-Express2pVitro2-neo-mcs pSecTag2A pCMV-DsRed-Express2pUB6/V5-His/LacZ pGL4.27pcDNA3.1/NT-GFP-TOPOpUB6/V5-His C pGL4.26pEF1α-IRES-ZsGreen1pUB6/V5-His B pACT pCMV-Tag2ApUB6/V5-His A pBIND-Id Control pCMV-Tag5BpTracer-CMV2pTRE2pAcGFP1-C In-Fusion ReadypSV-β-Galactosidase pRevTRE p3XFLAG-CMV-14pSI pTK-hyg p3XFLAG-CMV-8pSG5pTRE3G-Luc pFLAG-CMV-2pSFV1pSwitch pcDNA3.3-TOPOpSecTag2/Hygro A pcDNA4/His C pcDNA6.2/cLumio-DESTpSecTag B c-Flag pcDNA3pCMV-tdTomatopRluc-N2pcDNA4/TO/Myc-His A pAcGFP1-MitopPICZalpha D pcDNA6/myc-His B pAcGFP1-N In-Fusion Ready pORF-lacZ pcDNA6/V5-His B pDsRed-Monomer-N In-Fusion Ready pORF-HSV1tk pcDNA6.2/nTC-Tag-DEST pcDNA4/TO/Myc-His BpOG44pOptiVEC-TOPO pIRES2-EGFPpNTAP-B pcDNA5/FRT pcDNA3.1/His CpMEP4pGL4.30pcDNA3.1/CT-GFP-TOPOpLVX-ZsGreen-miRNA-Puro pGL4.19pEF1α-IRES-AcGFP1pLVX-IRES-Puro-3xFlag pACT-MyoD pcDNA3.2/V5/GW/D-TOPO pLPCX pCMV-BD pcDNA4/TO/Myc-His/LacZ pLEGFP-N1pCMV-Tet3G pcDNA4/HisMax-TOPOpKH3pTet on advanced p3XFLAG-CMV-13pIRES-puro2pTRE-Tight p3xFLAG-CMV-10phRL-TK pIND pFLAG-CMV-3pG5lac pGene/V5-His B pcDNA4/TO/Myc-His CpFR-luc pOPRSVI pcDNA6.2/C-YFP-DESTpEF6/myc-His C pcDNA4/HisMax A pcDNA6.2/cTC-Tag-DESTpEF4/V5-His A pIRESpuro3pcDNA6.2/nGeneBLAzer-DESTpEF1/myc-His lacZ pIRESneo3pCRE-MetLuc2-ReporterpEF1/myc-His C pIRESneo2pEF1α-DsRed-Express2pEF1/myc-His B pcDNA4/myc-His A pDsRed-Express-C1pEF1/myc-His A pCMV-PKA pEF1α-DsRed-Monomer-N1 pECFP-Mito pAcGFP1-N3pDD-AmCyan1ReporterpECFP-ER pcDNA5/FRT/TO pCRE-DD-AmCyan1pDP8rs pBApo-CMV-Pur pIRES2-DsRed-Express2pDP5rs pBApo-EF1α-pur pDsRed-Express-N1pDP4rs ptdTomato-N1pcDNA6.2/nLumio-DESTpDP3rs pAcGFP1-Golgi pcDNA6/myc-His ApDP2rs pAcGFP1-p53pcDNA6/V5-His ApDP1rs pAcGFP1-Actin pcDNA5/FRT/TO-TOPOpCMV-Myc-C pBI-CMV2pcDNA6.2/V5/GW/D-TOPOpCMV-HA-N pEF1α-tdTomato pcDNA6.2/cGeneBLAzer-DEST pCMV-HA-C pEF1α-tdTomato pcDNA6.2/nGeneBLAzer-GW/D-TOPO pCMV6-XL4pEBVHis A pcDNA5/TOpCMV6-AC-GFP pGL4.10pBApo-CMV-neopCMV5pGL4.29pDsRed-MonomerpCI pGL4.13pIRESpCGN pG5luciferase pIRES-hrGFP-1apcDNA6.2/EmGFP-Bsd/V5-DEST pCMV-AD pDsRed-Express2-N1pcDNA4/V5-His A pRevTet-Off pCMV-Tag3BpcDNA3-mRFP pTet-Off pCRE-hrGFPpcDNA3-CFP pTRE2-hygro pDsRED2-MitopcDNA3.1(+)/myc-His C pVgRxR pAcGFP1-FpcDNA3.1(+)/myc-His A pOPI3CAT pAcGFP1-C1pcDNA3.1(+)/CAT pBK-RSV pAsRed2-C1pcDNA3.1(-)/myc-His C pIRES2-DsRed2pAsRed2-N1pcDNA3.1/Zeo(+)pCMV-Myc pAcGFP1-LampcDNA3.1/Zeo(-)pCMV-Tag2C pAcGFP1-CpcDNA3.1/V5-His C pCMV-Tag5A pSEAP2-BasicpcDNA3.1/Hygro(+)pCMV-Tag3C pBI-CMV3pcDNA3.1/Hygro(-)p3XFLAG-CMV-7pNFkB-DD-tdTomatopcDNA3.1/GS p3XFLAG-CMV-9pcDNA3.1/His ApBS185CMV-Cre pFLAG-CMV-4pEBVHis BpBIND-GFP pBI-CMV4pGL4.75pBiFC-VN155(I152L)pcDNA4/His A pGL4.20pBiFC-CC155pcDNA4/myc-His B pCMV-SPORT6pBiFC-CrN173pcDNA4/HisMax C pCMV-SPORT6pBiFC-bJunVN155(I152L)pCMV-Tag4A pBINDpBD-p53pcDNA6/myc-His C pBD-NF-κBpBD-NF-kB pCMV-Tag2B pRevTet-OnpBC1pGRN145pTet-OnpAD-TRAF pCMV-MEK1pTRE3GpAD-SV40T pCMV-Tag3A pcDNA4/TOpAAV-ZsGreen-miRNA pCMVLacI pcDNA4/His B pAAV-tTS-shRNA pBI-CMV1pcDNA4/HisMax B pIREShyg3pEF1α-AcGFP1-N1pcDNA4/myc-His C pcDNA6/TR pCMV-LacZ pcDNA3.1/His B pDsRed-Express2-C1pCMV-Tag4B pcDNA6/V5-His C pBI-CMV5pCMV-Tag5C pCHO1.0 pAmCyan1-C1plRES2-ZsGreen1pGL3-Promoter pAcGFP1-Mem p3XFLAG-CMV-7.1pCMV-MEKK1 pAcGFP1-C3pFLAG-CMV-5a pFLAG-CMV2 pTT5pBudCE4.1pAcGFP1-C2 pSEAP2-Control pREP4ptdTomato-C1 pIRES2-AcGFP1pBApo-CMV pCRE-DD-tdTomato pAcGFP1-Hyg-C1。
载体与引物表(完全)
SP6 PYES2.R
M13R T3
载体抗性 Amp+
Amp+ Amp+ Amp+ Amp+ Amp+ Amp+ Amp+ Amp+
Amp+ Amp+ Amp+ Amp+
共3第3页
PM
PM-F
pMAL-c2E
MalE primer
pMAL-C2x
P5’
pMAL-p2X
MalE primer
pMD18-T
PQE30F/M13R(-48)/RV-M
Pmep4
pMEP4-F
pPIC9K(A+,Z+)
5’AOX(pPIC9K-F)
PPICZa pPROEXHTA
5'AOX/PPICZa-F M13R(-48)
pcDNA3.1R BGH
Amp+
M13F
M13R
pCEP-F pCI-F2/ T7(17base)
EBV-R (此载体没有反向引物)EBR
pCI-F2/ T7(19/17base)
T3
Amp+
T7 2G
T3
pCMV-F
pCMV-R
T3 /PFLAG-CMV-F
T7
Pcmv5f
Pcmv5r
Amp+
Pcmv5f
PDC316.R Primer(R)
载体抗性
pDonar
M13F
M13R
pDONR207
FORWARD
REVERSE
Pdonr221
M13F
pBD-NF-kB使用说明
pBD-NF-kB编号载体名称北京华越洋VECT76065pBD-NF-kBpBD-NF-kB载体图谱:pBD-NF-kB载体相关的哺乳动物表达载体：SuperCos I pDsRed2-Bid pNFκB-MetLuc2-Reporter pYr-adshuttle-3pAcGFP1-N1pEF1α-IRES-DsRed-Express2 pVitro2-neo-mcs pSecTag2A pCMV-DsRed-Express2 pUB6/V5-His/LacZ pGL4.27pcDNA3.1/NT-GFP-TOPO pUB6/V5-His C pGL4.26pEF1α-IRES-ZsGreen1 pUB6/V5-His B pACT pCMV-Tag2ApUB6/V5-His A pBIND-Id Control pCMV-Tag5BpTracer-CMV2pTRE2pAcGFP1-C In-Fusion ReadypSV-β-Galactosidase pRevTRE p3XFLAG-CMV-14pSI pTK-hyg p3XFLAG-CMV-8pSG5pTRE3G-Luc pFLAG-CMV-2pSFV1pSwitch pcDNA3.3-TOPOpSecTag2/Hygro A pcDNA4/His C pcDNA6.2/cLumio-DESTpSecTag B c-Flag pcDNA3pCMV-tdTomatopRluc-N2pcDNA4/TO/Myc-His A pAcGFP1-MitopPICZalpha D pcDNA6/myc-His B pAcGFP1-N In-Fusion Ready pORF-lacZ pcDNA6/V5-His B pDsRed-Monomer-N In-Fusion Ready pORF-HSV1tk pcDNA6.2/nTC-Tag-DEST pcDNA4/TO/Myc-His BpOG44pOptiVEC-TOPO pIRES2-EGFPpNTAP-B pcDNA5/FRT pcDNA3.1/His CpMEP4pGL4.30pcDNA3.1/CT-GFP-TOPOpLVX-ZsGreen-miRNA-Puro pGL4.19pEF1α-IRES-AcGFP1pLVX-IRES-Puro-3xFlag pACT-MyoD pcDNA3.2/V5/GW/D-TOPO pLPCX pCMV-BD pcDNA4/TO/Myc-His/LacZ pLEGFP-N1pCMV-Tet3G pcDNA4/HisMax-TOPOpKH3pTet on advanced p3XFLAG-CMV-13pIRES-puro2pTRE-Tight p3xFLAG-CMV-10phRL-TK pIND pFLAG-CMV-3pG5lac pGene/V5-His B pcDNA4/TO/Myc-His CpFR-luc pOPRSVI pcDNA6.2/C-YFP-DESTpEF6/myc-His C pcDNA4/HisMax A pcDNA6.2/cTC-Tag-DESTpEF4/V5-His A pIRESpuro3pcDNA6.2/nGeneBLAzer-DESTpEF1/myc-His lacZ pIRESneo3pCRE-MetLuc2-ReporterpEF1/myc-His C pIRESneo2pEF1α-DsRed-Express2pEF1/myc-His B pcDNA4/myc-His A pDsRed-Express-C1pEF1/myc-His A pCMV-PKA pEF1α-DsRed-Monomer-N1 pECFP-Mito pAcGFP1-N3pDD-AmCyan1ReporterpECFP-ER pcDNA5/FRT/TO pCRE-DD-AmCyan1pDP8rs pBApo-CMV-Pur pIRES2-DsRed-Express2pDP5rs pBApo-EF1α-pur pDsRed-Express-N1pDP4rs ptdTomato-N1pcDNA6.2/nLumio-DESTpDP3rs pAcGFP1-Golgi pcDNA6/myc-His ApDP2rs pAcGFP1-p53pcDNA6/V5-His ApDP1rs pAcGFP1-Actin pcDNA5/FRT/TO-TOPOpCMV-Myc-C pBI-CMV2pcDNA6.2/V5/GW/D-TOPOpCMV-HA-N pEF1α-tdTomato pcDNA6.2/cGeneBLAzer-DEST pCMV-HA-C pEF1α-tdTomato pcDNA6.2/nGeneBLAzer-GW/D-TOPO pCMV6-XL4pEBVHis A pcDNA5/TOpCMV6-AC-GFP pGL4.10pBApo-CMV-neopCMV5pGL4.29pDsRed-MonomerpCI pGL4.13pIRESpCGN pG5luciferase pIRES-hrGFP-1apcDNA6.2/EmGFP-Bsd/V5-DEST pCMV-AD pDsRed-Express2-N1pcDNA4/V5-His A pRevTet-Off pCMV-Tag3BpcDNA3-mRFP pTet-Off pCRE-hrGFPpcDNA3-CFP pTRE2-hygro pDsRED2-MitopcDNA3.1(+)/myc-His C pVgRxR pAcGFP1-FpcDNA3.1(+)/myc-His A pOPI3CAT pAcGFP1-C1pcDNA3.1(+)/CAT pBK-RSV pAsRed2-C1pcDNA3.1(-)/myc-His C pIRES2-DsRed2pAsRed2-N1pcDNA3.1/Zeo(+)pCMV-Myc pAcGFP1-LampcDNA3.1/Zeo(-)pCMV-Tag2C pAcGFP1-CpcDNA3.1/V5-His C pCMV-Tag5A pSEAP2-BasicpcDNA3.1/Hygro(+)pCMV-Tag3C pBI-CMV3pcDNA3.1/Hygro(-)p3XFLAG-CMV-7pNFkB-DD-tdTomatopcDNA3.1/GS p3XFLAG-CMV-9pcDNA3.1/His ApBS185CMV-Cre pFLAG-CMV-4pEBVHis BpBIND-GFP pBI-CMV4pGL4.75pBiFC-VN155(I152L)pcDNA4/His A pGL4.20pBiFC-CC155pcDNA4/myc-His B pCMV-SPORT6pBiFC-CrN173pcDNA4/HisMax C pCMV-SPORT6pBiFC-bJunVN155(I152L)pCMV-Tag4A pBINDpBD-p53pcDNA6/myc-His C pBD-NF-κBpBD-NF-kB pCMV-Tag2B pRevTet-OnpBC1pGRN145pTet-OnpAD-TRAF pCMV-MEK1pTRE3GpAD-SV40T pCMV-Tag3A pcDNA4/TOpAAV-ZsGreen-miRNA pCMVLacI pcDNA4/His BpAAV-tTS-shRNA pBI-CMV1pcDNA4/HisMax BpIREShyg3pEF1α-AcGFP1-N1pcDNA4/myc-His CpcDNA6/TR pCMV-LacZ pcDNA3.1/His BpDsRed-Express2-C1pCMV-Tag4B pcDNA6/V5-His CpBI-CMV5pCMV-Tag5C pCHO1.0pAmCyan1-C1plRES2-ZsGreen1pGL3-PromoterpAcGFP1-Mem p3XFLAG-CMV-7.1pCMV-MEKK1 pAcGFP1-C3pFLAG-CMV-5a pFLAG-CMV2 pTT5pBudCE4.1pAcGFP1-C2 pSEAP2-Control pREP4ptdTomato-C1 pIRES2-AcGFP1pBApo-CMV pCRE-DD-tdTomato pAcGFP1-Hyg-C1。
所有质粒载体汇总
pEZZ18 pkk232-8,pkk 233-3,pACYC184,pBR322,pUC119 pTYB1,pTYB2,pTYB4,pTYB11 pBlueScript SK（+）,pBlueScript SK（-） pLLP ompA, pINIIIompA, pMBP-P ,pMBP-C, 大肠杆菌冷激质 粒： pColdI pColdII pColdIII pColdTF 原核共表达质粒：pACYCduet1,pETduet-1,pCDFduet-1，pRSFduet-1 Takara公司大肠杆菌分子伴 侣： pG-KJE8 pGro7 pKJE7 pGTf2 pTf16 大肠杆菌宿主细胞： DH5a JM101 JM103 JM105 JM107 JM109 JM110 Top10 Top10F BL21（DE3） HB101 ER2529 E2566 C2566 MG1655 XL-10gold XL blue M15 JF1125 K802 SG1117 BL21（AI） BL21（DE3）plysS TG1 TB1 DH5a（pir） Tuner（DE3） Bl21 codonplusRIPL Novablue（DE3） Rosetta Rosetta（DE3） Rosetta（DE3）plys Rosetta-gami（DE3） RosettagamiB（DE3）, Rosetta-gamiB（DE3）plysS Orgami（DE3） OrgamiB（DE3） HMS174（DE3） 植物表达/RNAi载体农杆菌pBI121,pBI121-GFP,pBI101,pBI221,pSN1301, pUN1301,pRTL2 , pRTL2-GFP , pRTL2-CFP, pRTL2-RFP , pRTL2YFP,pCAMBIA 1300, 1301, 1302,1303,1304,1305, 1381Z,1391Z,2300, 2301,3300,3301,pCAMBIA super1300,pCAMBIA super1300GFP,pPZP212,pPZP2121,pPZP212-GFP,pGDG,RNAi载 体pART27,pHANNIBAL,pKANNIBAL, pFGC5941,pTCK303, pTRV1,pTRV2, T-DNA插入载体（随机突变体库）pSKI015,pSKI074,真 菌ATMT载体pBIG2RHPH2-GUS-GFP,pBHt1 枯草芽孢杆菌表达载体pWB980,pHT43,pHP13,pHP43, pBE2,pMUTIN4,pUB110,pE194,pMA5, pMK3,pMK4,pHT304,pHY300PLK, pBest502,pDG1363,pSG1154,pAX01, pSAS144,pDL,pDG148-stu,pDG641, pAL12,pUCX05-bgaB,pHT01， 配套 菌株BS 168,WB600,WB800,WB700, WB800N,1012,FZB42,1A747,广宿主 质粒pVLT33
哺乳动物细胞表达系统
30年前由HEK细胞系转化腺病毒5的裸露DNA
表达腺病毒13 S Ela 蛋白显著增强CMV启动子的转录
HEK293T
293细胞同时转化了SV40 TAg（大T抗原）
SV40 ori (293T)
和293细胞相比，没有显著增强表达
HEK293E
293细胞同时转化了Epstein-Barr virus (EBV) nuclear antigen-1 (EBNA1)
EBV oriP(293E)
和293细胞相比，显著增强表达
HKB11（人体细胞杂交细胞系）
HEK293细胞杂交淋巴瘤细胞而成。转化了EBNA1
EBV oriP/（HKB11）
用HIV-1 Tat/TAR反式作用因子载体。相比293能提高20倍
遗传标记
编码产物
筛选标记
作用机理
APH-
氨基糖苷转移酶
G418
APH灭火G418
DHFR
二氢叶酸还原酶
氨甲喋呤
DHFR变体抗氨甲喋呤
HPH-
潮霉素B磷酸转移酶
潮霉素B
HPH-灭活潮霉素
TK-
胸腺嘧啶核苷激酶
氨甲喋呤
TK合成TMP
XGPRT-
黄嘌呤鸟嘌呤磷酸核糖转移酶
霉酚酸
XGPRT-合成GMP
ADA-
腺嘌呤核苷脱氨酶
腺嘌呤木酮糖苷
ADA-灭活Xyl-A
载体标记
表达细胞
细胞
Hale Waihona Puke 起源特点应用Cos-1和COS-7（非洲绿猴肾细胞）
SV-1（非洲绿猴肾细胞）细胞系紫外照射和复制缺陷病毒SV40转化
表达SV40 TAg（大T抗原）允许具有SV40 ori 的载体大量复制
哺乳动物细胞表达系统
据文献报道，在不断提高的选择压力下，dhfr及侧翼序列 能扩增至上千个拷贝，大大增加目的基因的表达水平。
真核表达载体-启动子
外源基因在哺乳动物细胞中的表达与多种因素有关，主要 是启动子和增强子的强弱以及它们之间的搭配。
常用的高等哺乳动物受体细胞
迄今为止，用于医疗用品（药物、抗体、诊断试剂）大规 模生产的高等哺乳动物受体细胞主要还是中国仓鼠卵巢细 胞（CHO），其优势有如下几个方面： 遗传背景清楚，生理代谢稳定 与人的亲缘关系接近，外源蛋白修饰准确 基因转移和载体表达系统完善 耐受剪切力，便于大规模培养 被美国FDA确认为安全的基因工程受体细胞
Pei等用该细胞株表达分泌型的基质金属蛋白酶MMPI3，发现 高表达的阳性细胞克隆可占转染细胞的5％~l0％，其中一个 克隆的表达量可占细胞上清总蛋白的l5％~20％，在细胞单层 贴壁培养情况下表达量达10 mg/L。
对细胞株选择性地进行遗传改造
BHK/vl6细胞株是稳定表达单纯疱疹病毒(HSV)VP16蛋白 的BHK细胞，由于VP16的转录激活作用，载体中的HSV 早期启动子在该工程细胞中有很高的活性。
Clontech公司开发的Tet-off系统中的启动子则由CMV启动 子的核心序列和7个Tet阻遏蛋白结合位点组成。
这些启动子在诱导前后活性可相差4个数量级。
真核表达载体-启动子
在哺乳动物细胞中已发现存在大量在低氧环境中可诱导转录 的基因，如编码红细胞生成素(EPO)、转铁蛋白、血红素加 氧酶-1等的基因，它们都有一个共同的顺式作用元件 (CGTG )，有利于在5’或3’侧翼区的低氧诱导作用因子1(HIF-1)和低氧反应增强子(HRE)结合，激活靶基因的转录， 在低氧浓度下可使重组蛋白大量表达。
载体——精选推荐
NEB
原核表达 载体
NEB
配套大肠 杆菌
Amersham 原核表达载体
Amersham 原核表达载体
Amersham 原核表达载体
Invitrogen 原核表达载体
Lucigen
原核表达 菌株
Stratagene 原核表达菌株
Qiagen
原核表达 载体
Qiagen
原核表达 载体
Invitrogen 基因载体
700 元 现货 800 元 现货
900 元 现货
800 元 现货 800 元 现货 800 元 现货 800 元 现货 800 元 现货 700 元 现货 800 元 现货 800 元 现货 800 元 现货 800 元 现货 900 元 现货 1000 元 现货 1000 元 现货 1000 元 现货 900 元 现货 1000 元 现货 1500 元 现货 600 元 现货 300 元 现货 300 元 现货 600 元 现货 600 元 现货 800 元 现货 900 元 现货 900 元 现货 900 元 现货
BL21(DE3) BL21(DE3)plysS Rosetta(DE3) Rosetta(DE3)plysS Rosetta-gami(DE3) Rosetta-gami B(DE3)pLysS Orgami(DE3) OrgamiB(DE3) BLR(DE3) pTWIN1 pTYB11 pMXB10 pTXB1 ER2566 pGEX-6P-1 pGEX-4T-2 pGEX-4T-3 pRset-a C41(DE3) BL21 codonplus（DE3）RIPL TAGZyme pQE-2 pQE-Trisystem pOG44 pORF-lacZ pORF-HSV1tk pSP73 pUC18 pUC19 pBR322 pSP64 pBluescript II SK(+) SuperCos 1 pGAPZα A pPICZα A
所有质粒载体汇总
所有质粒载体汇总酿酒酵母表达载体pYES2,pYES2/NT,pYES2/CT,pYES3,pYES6, pYCplac22-GFP,酵母载体pAUR123,pRS303TEF,pRS304, pRS305,pRS306,pY13TEF,pY14TEF，pY15TEF，pY16TEF, 酵母基因重组表达载体pUG6, pSH47,酵母单杂载体pHISi,pLacZi,pHIS2, pGAD424, 酵母双杂交系统:酿酒酵母Y187, 酿酒酵母AH109；质粒pGADT7,pGBKT7；对照质粒pGBKT7-53，pGBKT7-lam，pGADT7-T，PCL1，酿酒酵母菌株INVSc1,YM4271, AH109,Y187,Y190,毕赤酵母表达载体pPIC9K,pPIC9K-His,pPIC3.5K,pPICZalphaA,B,C,pPICZA,B,C,pGAPZαA,pAO815,pPIC9k-His,pHIL-S1,pPink hc，配套毕赤酵母Pichiapink,毕赤酵母宿主X33，KM71，KM71H，GS115，原核表达载体pQE30,31,32,40,60,61,62,等原核表达载体,包括pET系列，pET-GST，pGEX系列（含GST标签），pMAL系列pMAL-c2x,-c4x,-c4e,-c5x,-p5x,pBAD,pBADHis,pBADmycHis系列，pQE系列,pTrc99a,pTrcHis系列，pBV220,221,222,pTXB系列，pLLP-ompA,pIN-III-ompA （分泌型表达系列）,pQBI63（原核表达带荧光）pET3a, pET 3d, pET 11a, pET 12a, pET 14b, pET 15b, pET 16b, pET 17b, pET 19b, pET 20b, pET 21a,b,d, pET 22b, pET 23a, pET 23b, pET 24a,b, pET 25b, pET 26b, pET 27b, pET 28a,b, pET 29a, pET 30a, pET 31b, pET 32a, pET 35b, pET 38b, pET 39b, pET 40b, pET 41a,b pET 42a, pET 43.1a,b pET 44a, pET 49b pET302,303 pET His,pET Dsb,pET GST,pET Trx pQE2, pQE9 pQE30,31,32, pQE 40pQE70 pQE80L pQETirs system pRSET-A pRSET-B pRSET-C pGEX4T-1,-2,-3,5x-1,6p-1,6p-2,2tk,3c pBV220,221,222 pTrcHisA,B,C pBAD24,34,43 pBAD HisA,B,C pPinPoint-Xa1,Xa2,Xa3 pMALc2x, p2x pBV220 pGEM Ex1, pGEM7ZF（+）, pTrc99A, pTwin1, pEZZ18 pkk232-8,pkk233-3,pACYC184,pBR322,pUC119pTYB1,pTYB2,pTYB4,pTYB11 pBlueScript SK （+）,pBlueScript SK （-）pLLP ompA, pINIIIompA, pMBP-P ,pMBP-C, 大肠杆菌冷激质粒： pColdI pColdII pColdIII pColdTF 原核共表达质粒：pACYCduet-1,pETduet-1,pCDFduet-1，pRSFduet-1 Takara公司大肠杆菌分子伴侣： pG-KJE8 pGro7 pKJE7 pGTf2 pTf16 大肠杆菌宿主细胞： DH5a JM101 JM103JM105 JM107 JM109 JM110 Top10 Top10F BL21（DE3）HB101 ER2529 E2566 C2566 MG1655 XL-10gold XL blue M15 JF1125 K802 SG1117 BL21（AI）BL21（DE3）plysS TG1 TB1 DH5a（pir）Tuner（DE3）Bl21 codonplusRIPL Novablue （DE3）Rosetta Rosetta（DE3）Rosetta（DE3）plys Rosetta-gami（DE3）Rosetta-gamiB（DE3）, Rosetta-gamiB（DE3）plysS Orgami （DE3）OrgamiB（DE3）HMS174（DE3）植物表达/RNAi载体农杆菌pBI121,pBI121-GFP,pBI101,pBI221,pSN1301,pUN1301,pRTL2 , pRTL2-GFP , pRTL2-CFP, pRTL2-RFP , pRTL2-YFP,pCAMBIA 1300, 1301, 1302,1303,1304,1305, 1381Z,1391Z,2300, 2301,3300,3301,pCAMBIA super1300,pCAMBIAsuper1300-GFP,pPZP212,pPZP2121,pPZP212-GFP,pGDG,RNAi载体pART27,pHANNIBAL,pKANNIBAL, pFGC5941,pTCK303, pTRV1,pTRV2,T-DNA插入载体（随机突变体库）pSKI015,pSKI074,真菌ATMT载体pBIG2RHPH2-GUS-GFP,pBHt1枯草芽孢杆菌表达载体pWB980,pHT43,pHP13,pHP43,pBE2,pMUTIN4,pUB110,pE194,pMA5,pMK3,pMK4,pHT304,pHY300PLK,pBest502,pDG1363,pSG1154,pAX01, pSAS144,pDL,pDG148-stu,pDG641,pAL12,pUCX05-bgaB,pHT01，配套菌株BS 168,WB600,WB800,WB700,WB800N,1012,FZB42,1A747,广宿主质粒pVLT33RNAi基因沉默干扰敲除载体pSilencer1.0，pSilencer 2.1-U6 hygro，pSilencer 3.1-H1 hygro，pSilencer 3.1-H1 neo，pSilencer 4.1-CMV neo， pSilencer 4.1-CMV puro pMIR-REPORT Luciferase RNAi载体（oligoengine）pSuper-puro RNAi逆转录病毒载体（clontech）: RNAi-Ready pSIREN-Retro Q， RNAi-Ready pSIREN-RetroQ-ZsGreen（Luciferase shRNA Annealed Oligonucleotide）RNAi慢病毒载体（addgene）: pLKO.1 哺乳动物表达载体pcDNA3.1+/-,pcDNA4/HisMax B，pSecTag2 A，pVAX1，pBudCE4.1,pTracer CMV2，pcDNA3.1（-）/myc-His A ，pcDNA6-Myc/His B，pCEP4， pIRES，pIRESneo，pIRES hyg3，pCMV-myc，pCMV-HA，pIRES-puro3，pIRES-neo3,pCAGGS哺乳动物双杂交系统pACT，pBIND，pACT-MyoD，pBIND-Id，pG5luc,pCMV-BD, pCMV-AD, pBD-p53, pFR-luc,Cytotrap Two-Hybrid System:pSos, pSos MAFB, pMyr蜕皮激素诱导系统pIND, pVgRxR,LacSwith II哺乳动物诱导表达系统:pOPRSVI ，pOPI3CAT，pCMVLacI,GeneSwitch System:pSwitch哺乳动物表面展示系统:pDisplay, 四环素调控系统（Invitrogen）：pcDNA4/TO/Myc-His A，pcDNA4/TO/Myc-His B，pcDNA4/TO/Myc-His C，pcDNA4/TO/Myc-His/LacZ，pcDNA6/TR四环素调控系统（Clontech）：pTet-On，pTet-Off，pTRE2,pRevTRE，pRevTet-On，pRevT et-off信号通路报告载体:pGAS-TA-Luc，pSTAT3-TA-Luc, pISRE-TA-Luc, pTA-Luc，pIκB-EGFP，pNFAT-TA-Luc，pCaspase3-sensor，pAP1（PMA）-Luc;pGL4.26[luc2P/minP/Hygro],pGL4.29[luc2P/CRE/Hygro],pGL 4.30[luc2P/NFA T-RE/Hygro]，pGL4.75;p53-Luc，pAP-1-Luc，pNF-κB-Luc，pSRE-Luc，pFA2-Elk1，pFC-MEKK，pFR-luc,Gateway系统（invitrogen）pcDNA6.2-GWEmGFP-miR negative, pLenti 6/TR,pcDNA 6.2-GW EmGFP-miR，乳酸菌表达载体及各种乳酸菌乳酸杆菌菌株，pNZ8148,pLEISS,pMG36e,pBBR1MCS-5,pBBR1MCS-6,pRV610,pLEM415,pHY3 00PLK,分泌型乳酸菌表达载体pVE5523，pPG611.1,pPG612.1等和乳酸杆菌菌株宿主菌NZ9000,MG1363,Lactobacillus casei 1.539,Lactobacillus casei,acidophilus NCFM,1.2,Lactobacillus sakei 23K,L.plantarum,L.rhamnosusGG,B.coagulans,Bifidobacterium bifidum,Bifidobacterium infantis,Lactococcus lactis M17,1663,Lactobacillus reuterii 广宿主表达载体链球菌表达敲除载体假单胞菌表达载体pVLT33,pBBR1MCS-2,3,4,5,6, pJRD215,pJN105,pME6032,Cos 载体pLAFR3,pMP2444（GFP）, pHY300PLK,pRT102,pRL1063a, 转座子载体pUT-miniTn5,pMGS100, pWHM10,pKC1139,pSET152,pOJ260,pPG611.1,pPG612.1，腺病毒载体/慢病毒，逆转录病毒表达载体及包装包膜质粒，腺病毒系统（Stratagene）: pAdEasy-1,pShuttle-CMV,pShuttle,pAdTrack, pAdTrack-CMV, pShuttle-IRES-hrGFP-1、pShuttle-IRES-hrGFP-2、pShuttle-CMV-lacZ,pShuttle-CMV-EGFP-C,pXC1, pBHGE3, 配套大肠杆菌BJ5183,293,293T cellline 腺相关病毒系统（Stratagene）：pAAV-MCS，pAAV-RC，pHelper，pAAV-LacZ，pAAV-IRES-hrGFP，pCMV-MCS，慢病毒载体:pLVX-DsRed-Monomer-N1,pLVX-IRES-ZsGreen1,pLVX-AcGFP1-N1,Lenti6/v 5-EDST-EGFP,pWPXL, FUGW,pLentilox 3.7,RNAi-Ready pSIREN-Retro Q,RNAi-Ready pSIREN-Retro Q-ZsGreen,pSUPER.Retro-GFP/Neo,pSUPER-Retro-Neo, pSUPER.Retro-puro,PLNCX PLNCX2 pMSCV-HYG pMSCV-neo pMSCV-puro pLEGFP-C1 pLOX-CW-CRE pLOX-GFP-IRES-TK pRetroX-IRES-DsRedExpress, pLVX-IRES-mCherry质粒载体。
pTT5哺乳动物表达载体说明
pTT5编号 载体名称北京华越洋生物VECT6098 pTT5pTT5载体基本信息载体名称: pTT5质粒类型: 哺乳动物细胞载体高拷贝/低拷贝: 高拷贝克隆方法: 限制性内切酶，多克隆位点启动子: CMV载体大小: 4401 bp5' 测序引物及序列: CMV-F:CGCAAATGGGCGGTAGGCGTG 3' 测序引物及序列: --载体标签: 无载体抗性: 氨苄青霉素筛选标记: --克隆菌株: DH5α等宿主细胞（系）: 常规细胞系（293、CV-1、CHO等）备注: 利用该质粒构建的基因表达是非融合表达，在构建质粒时需要在基因前面添加Kozak序列来增加蛋白表达效率。
稳定性: 瞬时表达组成型/诱导型: 诱导性病毒/非病毒: 非病毒pTT5载体质粒图谱和多克隆位点信息pTT5载体简介pTT5载体是很好的哺乳细胞瞬时蛋白表达载体，经常用于哺乳细胞的抗体轻链或者重链的表达。
pTT5载体序列ORIGIN1 GTACATTTAT ATTGGCTCAT GTCCAATATG ACCGCCATGT TGACATTGAT TATTGACTAG 61 TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT 121 TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC 181 GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG 241 GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG 301 TCCGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT 361 GACCTTACGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT 421 GGTGATGCGG TTTTGGCAGT ACACCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT 481 TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA 541 CTTTCCAAAA TGTCGTAATA ACCCCGCCCC GTTGACGCAA ATGGGCGGTA GGCGTGTACG 601 GTGGGAGGTC TATATAAGCA GAGCTCGTTT AGTGAACCGT CAGATCCTCA CTCTCTTCCG 661 CATCGCTGTC TGCGAGGGCC AGCTGTTGGG CTCGCGGTTG AGGACAAACT CTTCGCGGTC721 TTTCCAGTAC TCTTGGATCG GAAACCCGTC GGCCTCCGAA CGGTACTCCG CCACCGAGGG 781 ACCTGAGCGA GTCCGCATCG ACCGGATCGG AAAACCTCTC GAGAAAGGCG TCTAACCAGT 841 CACAGTCGCA AGGTAGGCTG AGCACCGTGG CGGGCGGCAG CGGGTGGCGG TCGGGGTTGT 901 TTCTGGCGGA GGTGCTGCTG ATGATGTAAT TAAAGTAGGC GGTCTTGAGA CGGCGGATGG 961 TCGAGGTGAG GTGTGGCAGG CTTGAGATCC AGCTGTTGGG GTGAGTACTC CCTCTCAAAA 1021 GCGGGCATTA CTTCTGCGCT AAGATTGTCA GTTTCCAAAA ACGAGGAGGA TTTGATATTC 1081 ACCTGGCCCG ATCTGGCCAT ACACTTGAGT GACAATGACA TCCACTTTGC CTTTCTCTCC 1141 ACAGGTGTCC ACTCCCAGGT CCAAGTTTAA ACGGATCTCT AGCGAATTCC CTCTAGAGGG 1201 CCCGTTTCTG CTAGCAAGCT TGCTAGCGGC CGCTCGAGGC CGGCAAGGCC GGATCCCCCG 1261 ACCTCGACCT CTGGCTAATA AAGGAAATTT ATTTTCATTG CAATAGTGTG TTGGAATTTT 1321 TTGTGTCTCT CACTCGGAAG GACATATGGG AGGGCAAATC ATTTGGTCGA GATCCCTCGG 1381 AGATCTCTAG CTAGAGGATC GATCCCCGCC CCGGACGAAC TAAACCTGAC TACGACATCT 1441 CTGCCCCTTC TTCGCGGGGC AGTGCATGTA ATCCCTTCAG TTGGTTGGTA CAACTTGCCA 1501 ACTGAACCCT AAACGGGTAG CATATGCTTC CCGGGTAGTA GTATATACTA TCCAGACTAA 1561 CCCTAATTCA ATAGCATATG TTACCCAACG GGAAGCATAT GCTATCGAAT TAGGGTTAGT 1621 AAAAGGGTCC TAAGGAACAG CGATGTAGGT GGGCGGGCCA AGATAGGGGC GCGATTGCTG 1681 CGATCTGGAG GACAAATTAC ACACACTTGC GCCTGAGCGC CAAGCACAGG GTTGTTGGTC 1741 CTCATATTCA CGAGGTCGCT GAGAGCACGG TGGGCTAATG TTGCCATGGG TAGCATATAC 1801 TACCCAAATA TCTGGATAGC ATATGCTATC CTAATCTATA TCTGGGTAGC ATAGGCTATC 1861 CTAATCTATA TCTGGGTAGC ATATGCTATC CTAATCTATA TCTGGGTAGT ATATGCTATC 1921 CTAATTTATA TCTGGGTAGC ATAGGCTATC CTAATCTATA TCTGGGTAGC ATATGCTATC 1981 CTAATCTATA TCTGGGTAGT ATATGCTATC CTAATCTGTA TCCGGGTAGC ATATGCTATC 2041 CTAATAGAGA TTAGGGTAGT ATATGCTATC CTAATTTATA TCTGGGTAGC ATATACTACC 2101 CAAATATCTG GATAGCATAT GCTATCCTAA TCTATATCTG GGTAGCATAT GCTATCCTAA 2161 TCTATATCTG GGTAGCATAG GCTATCCTAA TCTATATCTG GGTAGCATAT GCTATCCTAA 2221 TCTATATCTG GGTAGTATAT GCTATCCTAA TTTATATCTG GGTAGCATAG GCTATCCTAA 2281 TCTATATCTG GGTAGCATAT GCTATCCTAA TCTATATCTG GGTAGTATAT GCTATCCTAA 2341 TCTGTATCCG GGTAGCATAT GCTATCCTCA TGATAAGCTG TCAAACATGA GAATTAATTC 2401 TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT 2461 GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT 2521 ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT 2581 TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC 2641 CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA 2701 AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG 2761 TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT 2821 TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG 2881 CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC 2941 GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC 3001 GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA 3061 CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC 3121 AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT 3181 AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA 3241 TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA 3301 ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA3361 GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA3421 TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT3481 TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT3541 GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG3601 AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT3661 AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA3721 AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC3781 TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC3841 ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT3901 TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG3961 GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA4021 GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT4081 AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA4141 TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC4201 GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC4261 CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA4321 CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG4381 CGAGTCAGTG AGCGAGGAAG C//其他哺乳动物表达载体：pCHO1.0 pBApo-CMV-Pur pOPRSVIpcDNA3.1/His C pcDNA5/FRT/V5-His-TOPO pREP4pcDNA3.1/His B pcDNA5/FRT/TO-TOPO pDual-GCpcDNA3.1/His A pcDNA5/TO pBK-RSVpIRESpuro3 pcDNA5/FRT/TO pBK-CMVpIRES2-EGFP pcDNA5/FRT pBI-CMV4pTT5 pFLAG-CMV2 pcDNA4/TO/Myc-His/LacZ pNFkB-DD-tdTomato pcDNA3.1/CT-GFP-TOPO pOPI3CATpBI-CMV5 pcDNA3.1/NT-GFP-TOPO pGene/V5-His B pSEAP2-Basic pOptiVEC-TOPO pSwitchpSEAP2-Control pCMV-MEKK1 pCMVLacIpBI-CMV3 pCMV-MEK1 pVgRxRpBI-CMV2 pCMV-PKA pINDpBI-CMV1 pcDNA6.2/nTC-Tag-DEST pTRE3G-LucpNFκB-MetLuc2-Reporter pcDNA6.2/cTC-Tag-DEST pTRE3GpCRE-MetLuc2-Reporter pcDNA3.2/V5/GW/D-TOPO pTRE2-hygropAcGFP1-Actin pcDNA6.2/V5/GW/D-TOPO pTRE-TightpAcGFP1-N In-Fusion Ready pcDNA6.2/nGeneBLAzer-GW/D-TOPO pTK-hygpAcGFP1-C3 pcDNA6.2/C-YFP-DEST pTet-OnpAcGFP1-C pcDNA6.2/cGeneBLAzer-DEST pTet-OffpAcGFP1-p53 pcDNA6/V5-His A pTet on advanced pAcGFP1-Mito pcDNA6/V5-His B pRevTREpAcGFP1-Mem pcDNA6/V5-His C pRevTet-OnpAcGFP1-Lam pcDNA6/myc-His C pRevTet-Off pAcGFP1-Golgi pcDNA6/myc-His A pCMV-Tet3G pAcGFP1-F pcDNA6/myc-His B pTRE2pAcGFP1-Hyg-C1 pcDNA6.2/nGeneBLAzer-DEST pBD-NF-κB pAsRed2-N1 pcDNA4/HisMax-TOPO pCMV-AD ptdTomato-N1 pcDNA6.2/nLumio-DEST pCMV-BDpCMV-tdTomato pcDNA6.2/cLumio-DEST pBIND-Id Control pCRE-DD-tdTomato pcDNA4/myc-His C pBINDpCMV-DsRed-Express2 pcDNA4/HisMax C pG5 luciferasepEF1α-tdTomato pcDNA4/HisMax A pACT-MyoDpCRE-hrGFP c-Flag pcDNA3 pACTptdTomato-C1 pcDNA4/HisMax B pCMV-SPORT6 pAsRed2-C1 pcDNA4/myc-His B pGL4.13pGL3-Promoter pcDNA4/myc-His A pGL4.19pGL3 basic pcDNA4/His C pGL4.26pAcGFP1-C2 pcDNA4/His B pGL4.20pAcGFP1-C1 pcDNA4/His A pGL4.29pAcGFP1-N3 pcDNA6/TR pGL4.30pAcGFP1-N2 pcDNA4/TO/Myc-His A pGL4.27pAcGFP1-N1 pcDNA4/TO pGL4.75pAcGFP1-C In-Fusion Ready pcDNA4/TO/Myc-His B pGL4.10pCRE-DD-AmCyan1 pcDNA4/TO/Myc-His C pGRN145pNFkB-DD-AmCyan1 pcDNA3.3-TOPO pSecTag2 A pDsRed2-Bid pBudCE4.1 pEBVHis B pDsRED2-Mito pFLAG-CMV-4 pEBVHis ApDD-AmCyan1 Reporter pFLAG-CMV-3 pCMV-Tag 3C pAmCyan1-N1 pFLAG-CMV-2 pCMV-Tag 3A pAmCyan1-C1 pFLAG-CMV-5a pCMV-Tag 3BpEF1α-IRES-DsRed-Express2 p3XFLAG-CMV-9 pCMV-Tag 5CpEF1α-DsRed-Monomer-N1 p3xFLAG-CMV-10 pCMV-Tag 5A pDsRED-Monomer-N1 p3XFLAG-CMV-8 pCMV-Tag 4A pDsRed-Express-N1 p3XFLAG-CMV-7.1 pCMV-Tag 5Bp3XFLAG-CMV-7 pDsRed-Monomer-N In-Fusion Ready pCMV-Tag 4B pDsRed-Express-C1 p3XFLAG-CMV-13 pCMV-Tag 2C pIRES2-ZsGreen1 p3XFLAG-CMV-14 pCMV-Tag 2B pDsRed-Express2-C1 plRES2-ZsGreen1 pCMV-Tag 2A pDsRed-Express2-N1 pBApo-EF1α-pur pCMV-LacZpEF1α-DsRed-Express2 pBApo-EF1α-neo pCMV-MycpIRES2-DsRed-Express pBApo-CMV pEF1α-IRES-AcGFP1 pIRES2-DsRed-Express2 pBApo-CMV-neo pEF1α-IRES-ZsGreen1 pIRES-hrGFP-1a pIRES-EGFP pEF1α-AcGFP1-N1 pIRESneo2 pIRESneo3 pIRES2-DsRed2 pIRESneo pDsRed-Monomer pIRES2-AcGFP1 pIREShyg3 pIRES。
所有质粒载体汇总
酿酒酵母表达载体pYES2,pYES2/NT,pYES2/CT,pYES3,pYES6, pYCplac22-GFP,酵母载体pAUR123,pRS303TEF,pRS304, pRS305,pRS306,pY13TEF,pY14TEF，pY15TEF，pY16TEF,酵母基因重组表达载体pUG6, pSH47,酵母单杂载体pHISi,pLacZi,pHIS2, pGAD424, 酵母双杂交系统:酿酒酵母Y187, 酿酒酵母AH109；质粒pGADT7,pGBKT7；对照质粒pGBKT7-53，pGBKT7-lam，pGADT7-T，PCL1，酿酒酵母菌株INVSc1,YM4271, AH109,Y187,Y190,毕赤酵母表达载体pPIC9K,pPIC9K-His,pPIC3.5K,pPICZalphaA,B,C,pPICZA,B,C,pGAPZαA,pAO815,pPIC9k-His,pHIL-S1,pPink hc，配套毕赤酵母Pichiapink,毕赤酵母宿主X33，KM71，KM71H，GS115，原核表达载体pQE30,31,32,40,60,61,62,等原核表达载体,包括pET系列，pET-GST，pGEX系列（含GST标签），pMAL系列pMAL-c2x,-c4x,-c4e,-c5x,-p5x,pBAD,pBADHis,pBADmycHis系列，pQE系列,pTrc99a,pTrcHis系列，pBV220,221,222,pTXB系列，pLLP-ompA,pIN-III-ompA （分泌型表达系列）,pQBI63（原核表达带荧光）pET3a, pET 3d, pET 11a, pET 12a, pET 14b, pET 15b, pET 16b, pET 17b, pET 19b, pET 20b, pET 21a,b,d, pET 22b, pET 23a, pET 23b, pET 24a,b, pET 25b, pET 26b, pET 27b, pET 28a,b, pET 29a, pET 30a, pET 31b, pET 32a, pET 35b, pET 38b, pET 39b, pET 40b, pET 41a,b pET 42a, pET 43.1a,b pET 44a, pET 49b pET302,303 pET His,pET Dsb,pET GST,pET Trx pQE2, pQE9 pQE30,31,32, pQE 40 pQE70 pQE80L pQETirs system pRSET-A pRSET-B pRSET-C pGEX4T-1,-2,-3,5x-1,6p-1,6p-2,2tk,3c pBV220,221,222 pTrcHisA,B,C pBAD24,34,43 pBAD HisA,B,C pPinPoint-Xa1,Xa2,Xa3 pMALc2x, p2x pBV220 pGEM Ex1, pGEM7ZF（+）, pTrc99A, pTwin1, pEZZ18 pkk232-8,pkk233-3,pACYC184,pBR322,pUC119 pTYB1,pTYB2,pTYB4,pTYB11 pBlueScript SK （+）,pBlueScript SK（-）pLLP ompA, pINIIIompA, pMBP-P ,pMBP-C, 大肠杆菌冷激质粒： pColdI pColdII pColdIII pColdTF 原核共表达质粒：pACYCduet-1,pETduet-1,pCDFduet-1，pRSFduet-1 Takara公司大肠杆菌分子伴侣： pG-KJE8 pGro7 pKJE7 pGTf2 pTf16 大肠杆菌宿主细胞： DH5a JM101 JM103JM105 JM107 JM109 JM110 Top10 Top10F BL21（DE3）HB101 ER2529 E2566 C2566 MG1655 XL-10gold XL blue M15 JF1125 K802 SG1117 BL21（AI）BL21（DE3）plysS TG1 TB1 DH5a（pir）Tuner（DE3）Bl21 codonplusRIPL Novablue （DE3）Rosetta Rosetta（DE3）Rosetta（DE3）plys Rosetta-gami（DE3）Rosetta-gamiB（DE3）, Rosetta-gamiB（DE3）plysS Orgami（DE3）OrgamiB（DE3）HMS174（DE3）植物表达/RNAi载体农杆菌pBI121,pBI121-GFP,pBI101,pBI221,pSN1301,pUN1301,pRTL2 , pRTL2-GFP , pRTL2-CFP, pRTL2-RFP , pRTL2-YFP,pCAMBIA 1300, 1301, 1302,1303,1304,1305, 1381Z,1391Z,2300, 2301,3300,3301,pCAMBIA super1300,pCAMBIAsuper1300-GFP,pPZP212,pPZP2121,pPZP212-GFP,pGDG,RNAi载体pART27,pHANNIBAL,pKANNIBAL, pFGC5941,pTCK303, pTRV1,pTRV2,T-DNA插入载体（随机突变体库）pSKI015,pSKI074,真菌ATMT载体pBIG2RHPH2-GUS-GFP,pBHt1枯草芽孢杆菌表达载体pWB980,pHT43,pHP13,pHP43,pBE2,pMUTIN4,pUB110,pE194,pMA5, pMK3,pMK4,pHT304,pHY300PLK,pBest502,pDG1363,pSG1154,pAX01, pSAS144,pDL,pDG148-stu,pDG641,pAL12,pUCX05-bgaB,pHT01，配套菌株BS 168,WB600,WB800,WB700,WB800N,1012,FZB42,1A747,广宿主质粒pVLT33RNAi基因沉默干扰敲除载体pSilencer1.0，pSilencer 2.1-U6 hygro， pSilencer 3.1-H1 hygro，pSilencer 3.1-H1 neo， pSilencer 4.1-CMV neo， pSilencer 4.1-CMV puro pMIR-REPORT Luciferase RNAi载体（oligoengine）pSuper-puro RNAi逆转录病毒载体（clontech）: RNAi-Ready pSIREN-Retro Q， RNAi-ReadypSIREN-RetroQ-ZsGreen（Luciferase shRNA Annealed Oligonucleotide）RNAi慢病毒载体（addgene）: pLKO.1哺乳动物表达载体pcDNA3.1+/-,pcDNA4/HisMax B，pSecTag2 A，pVAX1，pBudCE4.1,pTracer CMV2，pcDNA3.1（-）/myc-His A ，pcDNA6-Myc/His B，pCEP4， pIRES，pIRESneo，pIRES hyg3，pCMV-myc，pCMV-HA，pIRES-puro3，pIRES-neo3,pCAGGS哺乳动物双杂交系统pACT，pBIND，pACT-MyoD，pBIND-Id，pG5luc,pCMV-BD, pCMV-AD, pBD-p53, pFR-luc,Cytotrap Two-Hybrid System:pSos, pSos MAFB, pMyr蜕皮激素诱导系统pIND, pVgRxR,LacSwith II哺乳动物诱导表达系统:pOPRSVI ，pOPI3CAT，pCMVLacI,GeneSwitch System:pSwitch哺乳动物表面展示系统:pDisplay, 四环素调控系统（Invitrogen）：pcDNA4/TO/Myc-His A，pcDNA4/TO/Myc-His B，pcDNA4/TO/Myc-His C，pcDNA4/TO/Myc-His/LacZ，pcDNA6/TR四环素调控系统（Clontech）：pTet-On，pTet-Off，pTRE2,pRevTRE，pRevTet-On，pRevTet-off信号通路报告载体:pGAS-TA-Luc，pSTAT3-TA-Luc, pISRE-TA-Luc, pTA-Luc，pIκB-EGFP，pNFAT-TA-Luc，pCaspase3-sensor，pAP1（PMA）-Luc;pGL4.26[luc2P/minP/Hygro],pGL4.29[luc2P/CRE/Hygro],pGL4.30[luc2P/NFA T-RE/Hygro]，pGL4.75;p53-Luc，pAP-1-Luc， pNF-κB-Luc，pSRE-Luc，pFA2-Elk1，pFC-MEKK，pFR-luc,Gateway系统（invitrogen）pcDNA6.2-GWEmGFP-miR negative, pLenti 6/TR,pcDNA 6.2-GW EmGFP-miR，乳酸菌表达载体及各种乳酸菌乳酸杆菌菌株，pNZ8148,pLEISS,pMG36e,pBBR1MCS-5,pBBR1MCS-6,pRV610,pLEM415,pHY3 00PLK,分泌型乳酸菌表达载体pVE5523，pPG611.1,pPG612.1等和乳酸杆菌菌株宿主菌NZ9000,MG1363,Lactobacillus casei 1.539,Lactobacillus casei,acidophilus NCFM,1.2,Lactobacillus sakei 23K,L.plantarum,L.rhamnosusGG,B.coagulans,Bifidobacterium bifidum,Bifidobacterium infantis,Lactococcus lactis M17,1663,Lactobacillus reuterii广宿主表达载体链球菌表达敲除载体假单胞菌表达载体pVLT33,pBBR1MCS-2,3,4,5,6, pJRD215,pJN105,pME6032,Cos载体pLAFR3,pMP2444（GFP）, pHY300PLK,pRT102,pRL1063a, 转座子载体pUT-miniTn5,pMGS100, pWHM10,pKC1139,pSET152,pOJ260,pPG611.1,pPG612.1，腺病毒载体/慢病毒，逆转录病毒表达载体及包装包膜质粒，腺病毒系统（Stratagene）: pAdEasy-1,pShuttle-CMV,pShuttle,pAdTrack, pAdTrack-CMV, pShuttle-IRES-hrGFP-1、pShuttle-IRES-hrGFP-2、pShuttle-CMV-lacZ,pShuttle-CMV-EGFP-C,pXC1, pBHGE3, 配套大肠杆菌BJ5183,293,293T cellline 腺相关病毒系统（Stratagene）： pAAV-MCS，pAAV-RC，pHelper，pAAV-LacZ，pAAV-IRES-hrGFP，pCMV-MCS，慢病毒载体:pLVX-DsRed-Monomer-N1,pLVX-IRES-ZsGreen1,pLVX-AcGFP1-N1,Lenti6/v 5-EDST-EGFP,pWPXL, FUGW,pLentilox 3.7,RNAi-Ready pSIREN-Retro Q,RNAi-Ready pSIREN-Retro Q-ZsGreen,pSUPER.Retro-GFP/Neo,pSUPER-Retro-Neo, pSUPER.Retro-puro,PLNCX PLNCX2 pMSCV-HYG pMSCV-neo pMSCV-puro pLEGFP-C1 pLOX-CW-CRE pLOX-GFP-IRES-TK pRetroX-IRES-DsRedExpress, pLVX-IRES-mCherry质粒载体。
哺乳动物基因表达Tol2载体是什么？
哺乳动物基因表达Tol2载体是什么？Tol2载体系统能高效地将外源DNA插入宿主细胞基因组中。
该系统技术简单，利用质粒转染（非病毒转导）将目的基因永久整合到宿主基因组中。
该系统来源于Tol2转座子，它最初是从硬骨鱼青鳉鱼（Oryzias latipes）中分离出来的。
基于序列同源性分析，发现T ol2转座子与整个脊椎动物基因组中发现的非自主元件的hAT家族密切相关。
Tol2系统包含两个载体，一个载体被称为辅助质粒，负责编码转座酶；另一个载体被称为转座子质粒，包含两个反向末端重复序列（ITRs）以及两者之间的转座区域，需要被转座到宿主基因组中的目的基因就克隆在这个区域。
当辅助质粒和转座子质粒共转染靶细胞时，辅助质粒产生的转座酶将会识别转座子的两个ITR，然后将被转座区和两个ITR元件插入到宿主基因组中。
T ol2转座子在宿主细胞中的插入位点没有明显碱基偏好性，这不同于具有特定靶标位点的转座子系统。
如，piggyBac转座插入通常发生在包含TTAA序列的宿主染色体位点。
Tol2是II类转座子，通过“剪切—粘贴”的机制移动，从一个地方转座到另一个地方，而不留下序列本身（恰好相反，I类转座子是通过“复制—粘贴”的方式移动）。
Tol2通过“剪切-粘贴”机制以单拷贝进行整合，在每一个插入位点都会产生一个8 bp的重复序列。
有两种途径可将转座酶导入靶细胞。
第一种是将辅助质粒瞬时转染到细胞中，使转座酶的瞬时表达。
第二种是利用辅助质粒体外转录出Tol2 mRNA，然后注射到靶细胞中。
无论哪种情况，转座酶都只能短时间表达。
随着辅助质粒的丢失或转座酶mRNA的降解，转座子会永久整合到宿主基因组中。
如果将T ol2转座酶重新导入细胞中，整合的转座子会再次通过“剪切—粘贴”的机制移动。
pTRE3G-Luc哺乳动物表达载体说明
pTRE3G-Luc哺乳动物表达载体说明pTRE3G-Luc编号载体名称北京华越洋⽣物VECT6088 pTRE3G--‐LucpTRE3G--‐Luc载体基本信息载体名称: pTRE3G-Luc质粒类型: 哺乳细胞表达；四环素调控载体⾼拷贝/低拷贝: --启动⼦: --克隆⽅法: 多克隆位点，限制性内切酶载体⼤⼩: 5075bp5' 测序引物及序列: --3' 测序引物及序列: --载体标签: --载体抗性: 氨苄青霉素（Ampicillin)筛选标记: --备注: --稳定性: --组成型: --病毒/⾮病毒: --pTRE3G--‐Luc载体质粒图谱和多克隆位点信息其他哺乳动物表达载体：pCHO1.0 pBApo-CMV-Pur pOPRSVIpcDNA3.1/His C pcDNA5/FRT/V5-His-TOPO pREP4pcDNA3.1/His B pcDNA5/FRT/TO-TOPO pDual-GCpcDNA3.1/His A pcDNA5/TO pBK-RSVpIRESpuro3 pcDNA5/FRT/TO pBK-CMVpIRES2-EGFP pcDNA5/FRT pBI-CMV4pTT5 pFLAG-CMV2 pcDNA4/TO/Myc-His/LacZ pNFkB-DD-tdTomato pcDNA3.1/CT-GFP-TOPO pOPI3CAT pBI-CMV5 pcDNA3.1/NT-GFP-TOPO pGene/V5-His B pSEAP2-Basic pOptiVEC-TOPO pSwitchpSEAP2-Control pCMV-MEKK1 pCMVLacIpBI-CMV3 pCMV-MEK1 pVgRxRpBI-CMV2 pCMV-PKA pINDpBI-CMV1 pcDNA6.2/nTC-Tag-DEST pTRE3G-LucpNFκB-MetLuc2-Reporter pcDNA6.2/cTC-Tag-DEST pTRE3GpCRE-MetLuc2-Reporter pcDNA3.2/V5/GW/D-TOPO pTRE2-hygropAcGFP1-Actin pcDNA6.2/V5/GW/D-TOPO pTRE-TightpAcGFP1-N In-Fusion Ready pcDNA6.2/nGeneBLAzer-GW/D-TOPO pTK-hygpAcGFP1-C3 pcDNA6.2/C-YFP-DEST pTet-OnpAcGFP1-C pcDNA6.2/cGeneBLAzer-DEST pTet-OffpAcGFP1-p53 pcDNA6/V5-His A pTet on advanced pAcGFP1-Mito pcDNA6/V5-His B pRevTREpAcGFP1-Mem pcDNA6/V5-His C pRevTet-OnpAcGFP1-Lam pcDNA6/myc-His C pRevTet-OffpAcGFP1-Golgi pcDNA6/myc-His A pCMV-Tet3GpAcGFP1-F pcDNA6/myc-His B pTRE2pAcGFP1-Hyg-C1 pcDNA6.2/nGeneBLAzer-DEST pBD-NF-κBpAsRed2-N1 pcDNA4/HisMax-TOPO pCMV-ADptdTomato-N1 pcDNA6.2/nLumio-DEST pCMV-BDpCMV-tdTomato pcDNA6.2/cLumio-DEST pBIND-Id ControlpCRE-DD-tdTomato pcDNA4/myc-His C pBINDpCMV-DsRed-Express2 pcDNA4/HisMax C pG5 luciferasepEF1α-tdTomato pcDNA4/HisMax A pACT-MyoDpCRE-hrGFP c-Flag pcDNA3 pACTptdTomato-C1 pcDNA4/HisMax B pCMV-SPORT6 pAsRed2-C1 pcDNA4/myc-His B pGL4.13pGL3-Promoter pcDNA4/myc-His A pGL4.19pGL3 basic pcDNA4/His C pGL4.26pAcGFP1-C2 pcDNA4/His B pGL4.20pAcGFP1-C1 pcDNA4/His A pGL4.29pAcGFP1-N3 pcDNA6/TR pGL4.30pAcGFP1-N2 pcDNA4/TO/Myc-His A pGL4.27pAcGFP1-N1 pcDNA4/TO pGL4.75pAcGFP1-C In-Fusion Ready pcDNA4/TO/Myc-His B pGL4.10pCRE-DD-AmCyan1 pcDNA4/TO/Myc-His C pGRN145pNFkB-DD-AmCyan1 pcDNA3.3-TOPO pSecTag2 A pDsRed2-Bid pBudCE4.1 pEBVHis B pDsRED2-Mito pFLAG-CMV-4 pEBVHis ApDD-AmCyan1 Reporter pFLAG-CMV-3 pCMV-Tag 3C pAmCyan1-N1 pFLAG-CMV-2 pCMV-Tag 3A pAmCyan1-C1 pFLAG-CMV-5a pCMV-Tag 3BpEF1α-IRES-DsRed-Express2 p3XFLAG-CMV-9 pCMV-Tag 5CpEF1α-DsRed-Monomer-N1 p3xFLAG-CMV-10 pCMV-Tag 5A pDsRED-Monomer-N1 p3XFLAG-CMV-8 pCMV-Tag 4A pDsRed-Express-N1 p3XFLAG-CMV-7.1 pCMV-Tag 5Bp3XFLAG-CMV-7 pDsRed-Monomer-N In-Fusion Ready pCMV-Tag 4B pDsRed-Express-C1 p3XFLAG-CMV-13 pCMV-Tag2C pIRES2-ZsGreen1 p3XFLAG-CMV-14 pCMV-Tag 2B pDsRed-Express2-C1 plRES2-ZsGreen1 pCMV-Tag 2A pDsRed-Express2-N1 pBApo-EF1α-pur pCMV-LacZpEF1α-DsRed-Express2 pBApo-EF1α-neo pCMV-MycpIRES2-DsRed-Express pBApo-CMV pEF1α-IRES-AcGFP1 pIRES2-DsRed-Express2 pBApo-CMV-neo pEF1α-IRES-ZsGreen1 pIRES-hrGFP-1a pIRES-EGFP pEF1α-AcGFP1-N1 pIRESneo2 pIRESneo3 pIRES2-DsRed2 pIRESneo pDsRed-Monomer pIRES2-AcGFP1 pIREShyg3 pIRES。
猪瘟病毒E2蛋白重组杆状病毒灭活疫苗(WH-09株)说明书和内包装标签2020版
猪瘟病毒E2蛋白重组杆状病毒灭活疫苗（WH-09株）说明书和内包装标签（一）猪瘟病毒E2蛋白重组杆状病毒灭活疫苗（WH-09株）说明书【兽药名称】通用名猪瘟病毒E2蛋白重组杆状病毒灭活疫苗（WH-09株）商品名无英文名Classical Swine Fever Virus E2 Protein Recombinant BaculovirusVaccine,Inactivated（Strain WH-09）汉语拼音Zhuwenbingdu E2 Danbai Chongzu Ganzhuangbingdu Miehuoyimiao (WH-09 Zhu)【主要成份及含量】主要成分为猪瘟病毒E2蛋白，每头份含量为50μg。
【性状】混悬液，久置可形成易摇散的沉淀。
【作用与用途】用于预防猪瘟。
免疫期为6个月。
【用法与用量】耳后颈部肌肉注射。
使用前将疫苗恢复至室温并充分摇匀，每次2ml（1头份）。
推荐免疫程序为：母猪产前6～7周接种2ml（1头份），21日后二免，所产仔猪在6～7周龄接种2ml（1头份），21日后二免；无母源抗体仔猪3～4周龄接种2ml（1头份），21日后二免。
【不良反应】无可见不良反应。
【注意事项】（1）本疫苗仅用于接种健康猪。
（2）使用前如发现包装破损、残缺、文字模糊、过期失效等，严禁使用。
（3）切勿冻结或长时间暴露于高温下。
（4）疫苗接种前应恢复至室温并充分摇匀，启封后一次用完。
（5）剩余疫苗及接种器具应做无害化处理。
【规格】（1）4ml/瓶（2）6ml/瓶（3）20ml/瓶（4）50ml/瓶（5）100ml/瓶（6）250ml/瓶【包装】(1)10瓶/盒(2) 1瓶/盒【贮藏与有效期】2～8℃保存，有效期为18个月。
【批准文号】【生产企业】仅在兽医指导下使用（二）猪瘟病毒E2蛋白重组杆状病毒灭活疫苗（WH-09株）内包装标签猪瘟病毒E2蛋白重组杆状病毒灭活疫苗（WH-09株）4（6、20、50、100、250）ml /瓶批准文号：批号：有效期至：【作用与用途】用于预防猪瘟。
- 1、下载文档前请自行甄别文档内容的完整性，平台不提供额外的编辑、内容补充、找答案等附加服务。
- 2、"仅部分预览"的文档,不可在线预览部分如存在完整性等问题,可反馈申请退款(可完整预览的文档不适用该条件!)。
- 3、如文档侵犯您的权益，请联系客服反馈,我们会尽快为您处理(人工客服工作时间：9:00-18:30)。
GTCGAGTTTA TATCAGTGAT GGTGGGAGGC ATCCACGCTG AATTCGAGCT CGATAAGCTT TGATTGTTCT CAGGGTGTTG TTGTTTCTTT TTCTGTAACT TCGTTTATTT GTAATTATAT TAGAATATTT CTACATCCTG GATGAGGATA CTTCTTTTTC CAAAGAATTC TGCCCTGGCT CATGAAGCCC AGTGTGTGGG AAAACATCAG ATGAACAAAG CCTTATTCCA TATTTTTTTC TCCTCTCCTG AATGAATCGG CGCTCACTGA AGGCGGTAAT AAGGCCAGCA TCCGCCCCCC CAGGACTATA CGACCCTGCC CTCAATGCTC GTGTGCACGA AGTCCAACCC GCAGAGCGAG ACACTAGAAG GAGTTGGTAG GCAAGCAGCA CGGGGTCTGA CAAAAAGGAT GTATATATGA CAGCGATCTG CGATACGGGA
2821 2881 2941 3001 3061 3121 3181 3241 3301 3361 3421 3481 3541 3601 3661 3721 3781 3841 3901 3961 4021 4081 4141 4201 4261 4321 4381 4441 4501 4561 4621 4681 4741 4801 4861 4921 4981 5041 5101 5161 5221 5281 //
TCAGTGATAG GAAAGTCGAG AGAGCTCGTT CATAGAAGAC GGATCCTCTA TCTCTAGAGC CTATTGTAAA GAAGATGTCC TCTGTTGACA AACTTTAGCT AAGTACTTTC CACACC TGGGCAACGT TGCAGGCTGC ACTGAGATCT TGACTTCTGG GTCTCTCACT TGGTTTAGAG AGAGGTCATC TTGACTTGAG CTAAAATTTT GTCATAGCTG GGGAGAGGCG TCGGTCGTTC ACAGAATCAG AACCGTAAAA CACAAAAATC GCGTTTCCCC TACCTGTCCG TATCTCAGTT CAGCCCGACC GACTTATCGC GGTGCTACAG GGTATCTGCG GGCAAACAAA AGAAAAAAAG AACGAAAACT ATCCTTTTAA TCTGACAGTT TCATCCATAG TCTGGCCCCA
GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCTAAGA AACCATTATT TAACCTATAA AAATAGGCGT ATCACGAGGC CCTTTCGTCT CGAGGCAGTG TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA CCATTATAAG CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG TTCAGGGGGA GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG CTGATTATGA CTCTATTCCT TTGCCCTCGG ACGAGTGCTG GGGCGTCGGT TTCCACTATC TCTACACAGC CATCGGTCCA GACGGCCGCG CTTCTGCGGG CGATTTGTGT GTCCCGGCTC CGGATCGGAC GATTGCGTCG CATCGACCCT GCGCCCAAGC AAATTGCCGT CAACCAAGCT CTGATAGAGT TGGTCAAGAC CAATGCGGAG CGGAGCCGCG GCGATCCTGC AAGCTCCGGA TGCCTCCGCT CGAAGTAGCG TCCATACAAG CCAACCACGG CCTCCAGAAG AAGATGTTGG CGACCTCGTA CCGAACATCG CCTCGCTCCA GTCAATGACC GCTGTTATGC GGCCATTGTC TTGTTGGAGC CGAAATCCGC GTGCACGAGG TGCCGGACTT CGGGGCAGTC AGCATCAGCT CATCGAGAGC CTGCGCGACG GACGCACTGA CGGTGTCGTC TGCCAGTGAT ACACATGGGG ATCAGCAATC GCGCATATGA AATCACGCCA TGACCGATTC CTTGCGGTCC GAATGGGCCG AACCCGCTCG TCTGGCTAAG GCGATCGCAT CCATGGCCTC CGCGACCGGC TGCAGAACAG CGGGCAGTTC AGGTCTTGCA ACGTGACACC CTGTGCACGG CGGGAGATGC AATAGGTCAG AATTCCCCAA TGTCAAGCAC TTCCGGAATC GGGAGCGCGG CCGATGCAAA ACATAACGAT CTTTGTAGAA ACCATCGGCG CAGCTATTTA CCCGCAGGAC CCTCCTACAT CGAAGCTGAA AGCACGAGAT TCTTCGCCCT CCGAGAGCTG GAGACGCTGT CGAACTTTTC GATCAGAAAC TTCTCGACAG ACGTCGCGGT TTTTTCATGG AAGCTTTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACA
181 241 301 361 421 481 541 601 661 721 781 841 901 961 1021 1081 1141 1201 1261 1321 1381 1441 1501 1561 1621 1681 1741 1801 1861 1921 1981 2041 2101 2161 2221 2281 2341 2401 2461 2521 2581 2641 2701 2761
pTRE2-hygro
编号 载体名称
北京华越洋生物 VECT6188 pTRE2-‐hygro pTRE2-hygro 载体基本信息 pTRE2-hygro 载体名称 : 哺乳细胞表达；四环素调控载体 质粒类型 : -高拷贝 /低拷贝 : -启动子 : 多克隆位点，限制性内切酶 克隆方法 : 5325bp 载体大小 : 5' 测序引物及序列 : -3' 测序引物及序列 : --载体标签 : 氨苄 载体抗性 : Hygromycin 筛选标记 : -备注 : 稳表达 稳定性 : 诱导型 组成型 : 非病毒 病毒 /非病毒 : pTRE2-‐hygro 载体质粒图谱和多克隆位点信息
AAAAGTGAAA TACCACTCCC AGGCGTGTAC TGGAGACGCC CGCGGCCCCG GCGGCCGCAT TGGGGACCCT GCAAAGTTTT CATGATAATT TCTTTTCATT AAATTCACTT GGCAATCAGG AATGATAAGG GTAGAAACAA CACTGTTTGA TTCATGCCTT ATCATTTTGG GTGTGGCCAA ATGGGGACAT TCATTGCAAT CAAATCATTT GCTGGCTGCC GCTGTCCATT TGTTTTGTGT AGATTTTTCC CGACTGCATT TCCGCTTCCT GCTCACTCAA ATGTGAGCAA TTCCATAGGC CGAAACCCGA TCTCCTGTTC GTGGCGCTTT AAGCTGGGCT TATCGTCTTG AACAGGATTA AACTACGGCT TTCGGAAAAA TTTTTTGTTT ATCTTTTCTA ATGAGATTAT TCAATCTAAA GCACCTATCT TAGATAACTA