基因诊断与基因治疗PPT课件(3)
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基因诊断与基因治疗
回顾：
1。细胞内决定生物性状的物质是什么？其功能 单位是什么？
2。不同的基因结构上的差异表现在哪些方面？ 3。什么碱基互补配对原则？ 4。现有某DNA片断上一条链上的碱基排列顺序
是AAGGCGTTA，你能写出另一条链上碱基 的排列顺序吗？
基因诊断
基因 蛋白质 性状
基因 诊断
生化 诊断
• We first bought a fridge and an electric rice cooker. My parents suggested we buy a microwave oven so that we can save a lot of time reheating food.
• My parents suggested we buy a microwave oven so that we can save a lot of time reheating food.
Then we bought a washing machine. After that, a vacuum cleaner came into our package as it is useful for keeping our house clean. We had a tiring but happy day.
Then we bought a washing machine. After that, a vacuum cleaner came into our package as it is useful for keeping our house clean. We had a tiring but happy day.
基因治疗
1。基因治疗： 将特定外源基因导入有基因缺陷的细
胞来治疗疾病 2。基因治疗过程： 选择治疗基因 治疗基因与载体结合
治疗基因正常表达
基因治疗对癌症的治疗方案
• 抑制癌细胞增生基因 导入癌细胞
• 抑制癌细胞转录 DNA片断导入癌细胞
阻断癌 细胞繁 殖
• 提高机体免疫力基因 导入免疫系统
提高免 疫力
• My family will move to a new flat. We need a lot of new household appliances before we can move in. Last Saturday, we went to a department store. The household appliances section is on the fifth floor. We first bought a fridge and an electric rice cooker.
Answers (1) for (2) at (3) until (4) by (5) during (6) to
Prepostions
Prepostions of time at , in ,for,by, since,during, before,after,
from, until Prepostions of place at ,in,on,above,against,behind, between,under,oppostite Prepostions of movement Across, along, down, into ,off, over,round, through, uder, up
• List all the prepositions in the story and think how they are used.
• My family will move to a new flat. We need a lot of new household appliances before we can move in. Last Saturday, we went to a department store. The household appliances section is on the fifth floor.
临床 诊断
基因诊断
基因诊断
源自文库
工具：
放射性同位素标记或荧光分子标记的 DNA分子探针
原理： 碱基互补配对原则（DNA分子杂交）
PCR技术：
在DNA聚合酶作用下，利用4种脱氧核苷酸， 依据模板DNA序列合成相同序列的DNA片断
1。DNA分子的复制需要什么条件？2。 哪4种脱氧核苷酸
基因诊断过程： 关键要素：
Prepositions are used before a noun or a noun phrase. And when a verb is used after a preposition, it is usually used in
its-ing form.
Read the dialogue on Page 8 and fill in the blanks with proper prepositions.
基因治疗
Unit 1 Living with technology
• Prepositions are words that are used before a noun or a noun phrase to show time, place, movement, etc.
• A preposition can also be used before a verb in the –ing form.
基因治疗的机理
• 基因置换：正常基因取代致病基因 • 基因修正：纠正致病基因的突变碱基序列 • 基因修饰：目的基因表达产物补偿致病基因
的功能 • 基因抑制：外源基因干扰、抑制有害基因的
表达 • 基因封闭：封闭特定基因的表达
小结
• 基本概念 基因诊断 PCR技术 基因芯片
• 基本理论 • 基因诊断过程 • 基因诊断及基因芯片的应用 • 基因治疗的过程 • 基因治疗的机理
探针DNA、目
基因诊断过程：
的DNA、信号
1. 制备基因探针
检测
2. 提取目的基因，获得单链DNA
3. PCR扩增DNA
4. 目的DNA与尼龙膜结合
5. 探针与目的DNA互补配对
6. 冲洗尼龙膜
7. 检测杂合分子
基因芯片
• 阅读课本，思考： – 什么是基因芯片？ – 基因芯片与基因诊断有什么关系？ – 基因芯片有什么优点？ – 基因芯片有哪些应用？
回顾：
1。细胞内决定生物性状的物质是什么？其功能 单位是什么？
2。不同的基因结构上的差异表现在哪些方面？ 3。什么碱基互补配对原则？ 4。现有某DNA片断上一条链上的碱基排列顺序
是AAGGCGTTA，你能写出另一条链上碱基 的排列顺序吗？
基因诊断
基因 蛋白质 性状
基因 诊断
生化 诊断
• We first bought a fridge and an electric rice cooker. My parents suggested we buy a microwave oven so that we can save a lot of time reheating food.
• My parents suggested we buy a microwave oven so that we can save a lot of time reheating food.
Then we bought a washing machine. After that, a vacuum cleaner came into our package as it is useful for keeping our house clean. We had a tiring but happy day.
Then we bought a washing machine. After that, a vacuum cleaner came into our package as it is useful for keeping our house clean. We had a tiring but happy day.
基因治疗
1。基因治疗： 将特定外源基因导入有基因缺陷的细
胞来治疗疾病 2。基因治疗过程： 选择治疗基因 治疗基因与载体结合
治疗基因正常表达
基因治疗对癌症的治疗方案
• 抑制癌细胞增生基因 导入癌细胞
• 抑制癌细胞转录 DNA片断导入癌细胞
阻断癌 细胞繁 殖
• 提高机体免疫力基因 导入免疫系统
提高免 疫力
• My family will move to a new flat. We need a lot of new household appliances before we can move in. Last Saturday, we went to a department store. The household appliances section is on the fifth floor. We first bought a fridge and an electric rice cooker.
Answers (1) for (2) at (3) until (4) by (5) during (6) to
Prepostions
Prepostions of time at , in ,for,by, since,during, before,after,
from, until Prepostions of place at ,in,on,above,against,behind, between,under,oppostite Prepostions of movement Across, along, down, into ,off, over,round, through, uder, up
• List all the prepositions in the story and think how they are used.
• My family will move to a new flat. We need a lot of new household appliances before we can move in. Last Saturday, we went to a department store. The household appliances section is on the fifth floor.
临床 诊断
基因诊断
基因诊断
源自文库
工具：
放射性同位素标记或荧光分子标记的 DNA分子探针
原理： 碱基互补配对原则（DNA分子杂交）
PCR技术：
在DNA聚合酶作用下，利用4种脱氧核苷酸， 依据模板DNA序列合成相同序列的DNA片断
1。DNA分子的复制需要什么条件？2。 哪4种脱氧核苷酸
基因诊断过程： 关键要素：
Prepositions are used before a noun or a noun phrase. And when a verb is used after a preposition, it is usually used in
its-ing form.
Read the dialogue on Page 8 and fill in the blanks with proper prepositions.
基因治疗
Unit 1 Living with technology
• Prepositions are words that are used before a noun or a noun phrase to show time, place, movement, etc.
• A preposition can also be used before a verb in the –ing form.
基因治疗的机理
• 基因置换：正常基因取代致病基因 • 基因修正：纠正致病基因的突变碱基序列 • 基因修饰：目的基因表达产物补偿致病基因
的功能 • 基因抑制：外源基因干扰、抑制有害基因的
表达 • 基因封闭：封闭特定基因的表达
小结
• 基本概念 基因诊断 PCR技术 基因芯片
• 基本理论 • 基因诊断过程 • 基因诊断及基因芯片的应用 • 基因治疗的过程 • 基因治疗的机理
探针DNA、目
基因诊断过程：
的DNA、信号
1. 制备基因探针
检测
2. 提取目的基因，获得单链DNA
3. PCR扩增DNA
4. 目的DNA与尼龙膜结合
5. 探针与目的DNA互补配对
6. 冲洗尼龙膜
7. 检测杂合分子
基因芯片
• 阅读课本，思考： – 什么是基因芯片？ – 基因芯片与基因诊断有什么关系？ – 基因芯片有什么优点？ – 基因芯片有哪些应用？