Rabeprazole_117976-89-3_DataSheet_MedChemExpress
Roche MagNA Lyser 产品说明书
Ordering Information Cat. No. Product ***********MagNA Lyser Instrument (230 Volt)***********MagNA Lyser Instrument (110 Volt)(Instruments supplied with rotor and rotor cooling block)***********MagNA Lyser Green Beads (100 tubes)Related Products Cat. No. Product***********MagNA Pure LC DNA Isolation Kit II (Tissue)***********MagNA Pure LC mRNA Isolation Kit II (Tissue)03 330 591 001MagNA Pure LC RNA Isolation Kit III (Tissue)***********MagNA Pure LC DNA Isolation Kit III (Bacteria, Fungi)***********MagNA Pure LC RNA Isolation Tissue Lysis Buffer – Refill (70 ml)System DescriptionHomogenize up to 16 samples in just a few seconds.Save valuable lab space with a small benchtop instrument.Reduce hands-on time by replacing the mortar and pestle and other manual methods.Integrate your workflow with the automated nucleic acid isolation of the MagNA Pure LC Instrument.Perform consistent and reproducible sample disruption.Process many different sample types.Prevent nucleic acid degradation with the benchtop cooling unit.Ease your setup with a removable rotor and prefilled disposable vials.Automate with an easy-to-use instrumentVersatile, efficient, and rapid pre-preparationFigure 71. Add your sample and lysis buffer to the MagNA Lyser Green Beads.2. Homogenize with the MagNA Lyser Instrument.3. Centrifuge to pellet the debris.4. Proceeed with the supernatant to prepare nucleic acids or proteins.For detailed information,visit or contact your local representative.Trademarks:MagNA Pure, MagNA Lyser, LightCycler, and the MagNA Pure Logo are trademarks of a member of the Roche Group.The technology used for the LightCycler System is licensed from Idaho Technology Inc., Salt Lake City, UT, USA.Fully automated sample preparationon the PCR Workflow SystemRoche Diagnostics GmbH Roche Applied Science Nonnenwald 282372 Penzberg Germany0000Roche Applied Science Part of Roche DiagnosticsMagNA Lyser InstrumentStart the Ball Rollingwith Automated Tissue HomogenizationᕤᕣᕢᕡFigure 6Components of the system.The MagNA Lyser InstrumentAutomated tissue homogenizationProcessing conditionsRefer to the following tables for guidelines on setting up your homogenizationSample material(10 mg)*Time settings(seconds)Cooling(between the runs)Speed Average yield(µg)***Average purity(OD 280/260 nm)***Spleen 2 x 25 906,00030–40 1.9Liver 25-6,00016–18 1.8Lung 2 x 25906,00025 1.8Kidney25-6,000201.8Maize leaves **20-5,00010n.d.Maize polenta **20-5,0008n.d.Tortilla chips **20-5,0001n.d.*Aliqout containing 10 mg sample material (here mouse and food samples) was taken for the DNA purificationusing the MagNA Pure LC DNA Isolation Kit II (Tissue), (see pack insert)**Centrifugation after the homogenization for 5 minutes at 2,200 x g*** Yield and purity strongly depend on the condition of the sample material n.d.not determinedData kindly provided by Dr. Peterhänsel, RWTH Aachen, GermanyFigure 1Gel electrophoresis from genomic DNA isolated from tissue homogenized with the MagNA LyserInstrument, using the MagNA Pure LC DNA Kit II (Tissue).Marker: DNA Marker III*Aliquot containing 10 mg sample material (here mouse and human research samples) was taken to purify RNAeither with the MagNA Pure LC RNA Isolation Kit III (Tissue) or the MagNA Pure LC mRNA Isolation Kit II (Tissue) homogenized with the MagNA Lyser Instrument.** Yield and purity strongly depend on the condition of the sample material. The yield for mRNA was not determined.Sample material(10 mg)*Time settings(cycles/seconds)Cooling(between/afterthe runs in seconds)SpeedAverage yield (mg)(total RNA)**Average purity(OD 280/260 nm)**RNA/mRNARarely expressed targets in small numbers of target cells,as seen in experiments about minimalresidual diseases,are difficult to detect.Increasing the cell number can improve sensitivity and lead to accurate results.Without the MagNA Lyser pre-processing,the MagNA Pure mRNA HS Kit can efficiently obtain mRNA from a maximum of 1 x 107white blood cells (WBCs),as shown in research studies with human samples.However,using greater cell numbers results in a saturation effect with quantitative assays (Figure 3).Homogenization of the lysate with the MagNA Lyser Instrument prior to the purification eliminatesthe amplification saturation at 1 x 107cells and allows the use of up to 2.5 x 107WBCs (Figure 4 and 5),enhancing the analytical sensitivity of the assay.Eliminate sensitivity barriers with increased sample inputFigure 3mRNA was purified from different amounts of human white blood cells with the MagNA Pure mRNA HS Kit. G6PDH was amplified using the LightCycler t(9;22) Quantification Kit (see text beside).Figure 4mRNA was purified from different amounts of human white blood cells with the MagNA Pure mRNA HS Kit. The lysates from 2.5 x 107cells and 5 x 107cells were homogenized with the MagNA Lyser Instrument (2x50 seconds with 90 seconds cooling in between) prior to the mRNA purification. G6PDH was amplified using the LightCycler t(9;22) Quantification Kit (see text beside).Figure 5Scalability from 1 x 106cells to 2.5 x 107cells is represented in the graph and the table of the relationship between crossing points and cell numbers. The limitation of cell input is indicated by no change in crossing point with increased cell number (see text beside).Cell number 5 x 1072.5 x 1071 x 1075 x 1061 x 106Log (cell number)7.77.47.06.76.0Crossing point 20.320.321.822.424.4crossingpointLog(cell number)252423222120195.86.36.87.37.8Figure 2Gel electrophoresis from total RNA isolated from tissue homogenized with the MagNA Lyser Instrument, using the MagNA Pure LC RNA Kit III (Tissue).Ma r k e rS p l e e nL i v e rL u n gK i d n e yM a r k e rMa i z e l e a v e sMa i z e l e a v e sS p l e e nL i v e r11 kb5 kb5 kb28 S rRNA 18 S rRNASpleen 2 x 50 90 6,500–7,000 30–40 1.9Liver 50 - 6,500–7,000 13–17 2.0Thymoid tissue60906,500n.d.n.d.Heart 60 90 6,500 n.d. n.d.Abdominal fat 60 90 6,500 n.d. n.d.Aorta 60 90 6,500 n.d. n.d. Other samples1+n x 50 90 6,500–7,000- -1 x 105 x 101 x 10- 5 x 101 x 105 x 105 x 10- 5 x 102.5 x 10 5 x 10。
Perkadox L-W75 粉型氯乙酰胺酸酯 USP 纯度产品数据表说明书
Product Data SheetPerkadox L-W75 Powder USP Grade Dibenzoyl peroxide, 75% with waterPerkadox® L-W75 Powder USP Grade is a free-flowing powder form of dibenzoyl peroxide suitable for use in various pharmaceutical applications including anti-acne creams, face and body washes, and shampoos.CAS number94-36-0EINECS/ELINCS No.202-327-6TSCA statuslisted on inventoryMolecular weight242.2Active oxygen contentperoxide6.61%Concentration4.95-5.28%ApplicationsPerkadox® L-W75 Powder USP Grade is a free-flowing granular form of dibenzoyl peroxide suitable for use in various pharmaceutical applications including anti-acne creams, face and body washes, and shampoos.Perkadox® L-W75 Powder USP Grade is manufactured in accordance with U.S. current Good Manufacturing Practices and relative regulations as they apply to Bulk Pharmaceutical Chemicals.Half-life dataThe reactivity of an organic peroxide is usually given by its half-life (t1/2) at various temperatures. The half-life of Perkadox® L-W75 Powder USP Grade in chlorobenzene is:0.1 hr at 113°C (235°F)1 hr at 91°C (196°F)10 hr at 71°C (160°F)Formula 1kd = A·e-Ea/RTFormula 2t½ = (ln2)/kdEa122.35 kJ/moleA 6.94E+13 s-1R8.3142 J/mole·KT(273.15+°C) KThermal stabilityOrganic peroxides are thermally unstable substances, which may undergo self-accelerating decomposition. The lowest temperature at which self-accelerating decomposition of a substance in the original packaging may occur is the Self-Accelerating Decomposition Temperature (SADT). The SADT is determined on the basis of the Heat Accumulation Storage Test.SADT80°C (176°F)Method The Heat Accumulation Storage Test is a recognized test method for thedetermination of the SADT of organic peroxides (see Recommendations on theTransport of Dangerous Goods, Manual of Tests and Criteria - United Nations, NewYork and Geneva).StorageDue to the relatively unstable nature of organic peroxides a loss of quality can be detected over a period of time. To minimize the loss of quality, Nouryon recommends a maximum storage temperature (Ts max.) for each organic peroxide product.Ts Max.40°C (104°F)Note When stored under these recommended storage conditions, Perkadox® L-W75Powder USP Grade will remain within the Nouryon specifications for a period of atleast 12 months after production.Packaging and transportPerkadox® L-W75 Powder USP Grade is packaged in a single 33.3 lb. HDPE bag in a protective box.Both packaging and transport meet the international regulations. For the availability of other packed quantities contact your Nouryon representative.Perkadox® L-W75 Powder USP Grade is classified as Organic peroxide type C; solid, Division 5.2; UN 3104.Safety and handlingKeep containers tightly closed. Store and handle Perkadox® L-W75 Powder USP Grade in a dry well-ventilated place away from sources of heat or ignition and direct sunlight. Never weigh out in the storage room.Avoid contact with reducing agents (e.g. amines), acids, alkalis and heavy metal compounds (e.g. accelerators, driers and metal soaps). Please refer to the Safety Data Sheet (SDS) for further information on the safe storage, use and handling of Perkadox® L-W75 Powder USP Grade. This information should be thoroughly reviewed prior to acceptance of this product.The SDS is available at /sds-search.All information concerning this product and/or suggestions for handling and use contained herein are offered in good faith and are believed to be reliable.Nouryon, however, makes no warranty as to accuracy and/or sufficiency of such information and/or suggestions, as to the product's merchantability or fitness for any particular purpose, or that any suggested use will not infringe any patent. Nouryon does not accept any liability whatsoever arising out of the use of or reliance on this information, or out of the use or the performance of the product. Nothing contained herein shall be construed as granting or extending any license under any patent. Customer must determine for himself, by preliminary tests or otherwise, the suitability of this product for his purposes.The information contained herein supersedes all previously issued information on the subject matter covered. The customer may forward, distribute, and/or photocopy this document only if unaltered and complete, including all of its headers and footers, and should refrain from any unauthorized use. Don’t copythis document to a website.Perkadox® is a registered trademark of Nouryon Chemicals B.V. or affiliates in one or more territories.Contact UsPolymer Specialties Americas************************Polymer Specialties Europe, Middle East, India and Africa*************************Polymer Specialties Asia Pacific************************2022-6-30© 2022Pharmaceutical industry Perkadox L-W75 Powder USP Grade。
Genebre S.A. Y-type filter 说明书
08908 L’HOSPITALET DE LLOBREGAT Barcelona(Spain)internet: http://www.genebre.esMANUAL DE INSTALACION Y OPERACIÓN DE LOS FILTROS EN “Y” CON CONEXIONES ROSCADAS.Leer toda la información e instrucciones antes de instalar el filtro. El incumplimiento de estas instrucciones puede provocar lesiones corporales o daños a la propiedad.RECEPCIÓN, MANIPULACIÓN E INSPECION1. Inspeccione los daños ocurridos durante el transporte. Informe de daños a la compañía de transporte. Si el filtro no se instala inmediatamente, almacenar el filtro en un lugar limpio y seco.2. Compruebe que la calificación del filtro es mayor o igual a la presión máxima y temperatura de la instalación.3. Inspeccionar la malla y eliminar cualquier material extraño o suelto que pueda ser arrastrado por el fluido.INSTALACIÓNPRECAUCIÓNAntes de la instalación, comprobar la aplicación y la compatibilidad química de los fluidos de proceso con el material de construcción del filtro. Compruebe que la calificación del filtro es mayor o igual a la presión máxima y temperatura de la instalación.PRECAUCIÓNPara las medidas de filtro más grandes, para levantar el filtro poner soportes bajo las conexiones de entrada y de salida.1. En el caso de que exista riesgo de incendio exterior, se debe montar el equipo adecuado para proteger el propio filtro y el resto de la instalación.2. Inspeccionar la malla y eliminar cualquier material extraño o suelto que pueda ser arrastrado por el fluido, antes de instalar el filtro.3. Coloque el filtro en la tubería de forma que el líquido entre en la dirección de la flecha de fundición grabada en el filtro. Siempre instale el filtro con el drenaje en la posición más baja.08908 L’HOSPITALET DE LLOBREGAT Barcelona(Spain)e-mail:******************internet:http://www.genebre.es4. Asegúrese de que se proporciona espacio suficiente para facilitar la apertura de la tapa del filtro y para sacar la malla del filtro.5. Para los filtros de mayor tamaño, los soportes que se coloquen para sostener el filtro deben evitar que las fuerzas y momentos de la tubería actúen sobre el mismo. La tubería debe permitir ajustes debido a las tolerancias del filtro.6. Conectar el filtro a la tubería utilizando las prácticas estándar de instalación en tuberías.7. Una vez instalado el filtro, las conexiones del mismo no deben de poder moverse libremente.PRECAUCIÓNEl filtro en “Y” no está diseñado para ser soporte de anclaje de la línea de tuberías.Asegúrese de apoyar adecuadamente las tuberías a ambos lados del filtro. Tenga cuidado para evitar que las fuerzas y momentos de las tuberías actúen sobre las conexiones del filtro. Pueden producirse daños al filtro si es conectado incorrectamente.8. Los filtros tipo “Y” no tienen rosca de acoplamiento para manómetros. Es necesario colocar un manómetro cerca de la entrada y otro a la salida del filtro para determinar la presión diferencial, para determinar la frecuencia de limpieza del filtro. Estos manómetros son esenciales para la operación segura del filtro y se deben instalar utilizando las prácticas estándar de instalación en tuberías.08908 L’HOSPITALET DE LLOBREGAT Barcelona(Spain)e-mail:******************internet: http://www.genebre.es PUESTA EN MARCHARealice la puesta en marcha de forma gradual. Esto elimina los golpes de ariete al filtro y a otros equipos de la línea.CUANDO LIMPIAR LA MALLAUn aumento de la presión diferencial a través del filtro de 0.3 a 0.7 bar indica que la malla está llena de suciedad y necesita ser limpiada.PRECAUCIÓNPara evitar daños en la malla, NO permitir que la diferencia de presión a través del filtro supere los 1.4 bar.RETIRAR, LIMPIAR O SUSTITUIR LA MALLA. PERIODOS DE PARO DE LA LINEA. PRECAUCIÓNAl abrir el filtro existe peligro debido a la presión interna de dicho filtro. El montado y desmontado del filtro tendrá que llevarse a cabo teniendo la precaución de eliminar lapresión en las conducciones. Una vez aislado el filtro de las conducciones, si el fluido está caliente esperar a que se enfríe antes de abrir el filtro. Siempre quedará líquido en la parte baja del filtro, que saldrá al abrir la tapa de éste. Para proteger al operador debe usar equipo de protección adecuado (gafas, guantes, chalecos, ropa, etc) en consonancia con el fluido de proceso, su presión y su temperatura.1. Poco a poco cerrar las válvulas aguas arriba del filtro. A continuación, abrir lentamente las válvulas tubería abajo del filtro. Después, cerrar también las válvulas tubería abajo del filtro. Asegúrese de que todas las válvulas están bien cerradas.2. El filtro tendrá en su interior presión interna y restos del fluido. Lentamente desenroscar la tapa y retirarla.3. Retirar los desechos de la malla. No permita que los desechos de la malla se sequen, ya que entonces sería difícil quitarlos y limpiar la malla.4. Invertir la malla y lavar los desechos mediante una corriente de aire o de agua en contra del exterior de la malla.5. Inspeccione cada uno de los agujeros de la malla para su limpieza. Reemplazar la malla si es necesario.6. Inspeccionar la junta de sellado de la tapa. Limpiar las superficies de sellado y la tapa.7. Limpiar el alojamiento de la malla. Colocar la malla en ángulo recto en el asiento de la tapa.8. Vuelva a colocar la tapa.9. Siga las instrucciones de puesta en marcha.。
SN-38_Topoisomerase_CAS号86639-52-3说明书_AbMole中国
0.15
1.8
0.4
0.08
10
体表面积 (m2)
0.007
0.025
0.15
0.05
0.02
0.5
Km 系数
3
6
12
8
5
20
动例物如，A依(m据g/体kg表) =面动积物折算B (法m，g/k将g白) ×藜动动芦物物醇BA用的的于KK小mm系系鼠数数的剂量22.4 （6），得到白藜芦醇用于大鼠的等效剂量为11.2 。 mg/kg
SN-38 目录号M3016
化学数据
分子量 分子式 号 CAS 储存条件
392.4 C22H20N2O5 86639-52-3
3年 -20°C 粉末状
溶解性（25°C）
DMSO 21 mg/mL Water <1 mg/mL EthanT-11的一种活性代谢物，抑制DNA topoisomerase I（DNA拓扑异构酶I） ， 为 IC50 1 μM，抑制DNA合成，且造成频繁的DNA单链断裂。SN-38是伊立替 康盐酸盐（CPT-11）的生物活性代谢物。SN-38引起DNA拓扑异构酶I的抑制最强，其次是CPT，然后是CPT-11。 CPT-11剂量依赖性地转移宽松的DNA在切口的 DNA的方向上的位置，但SN-38和CPT对宽松的DNA的位置没有影响。
实验操作 来自于公开的文献，仅供参考
细胞实验
细胞系
A-172, U-87, and LA-567
方法
MTT assay
浓度
0 -1000 nM
处理时间
48 h
动物实验 动物模型 配制 剂量 给药处理
不同实验动物依据体表面积的等效剂量转换表（数据来源于FDA指南）
苯丙氨酸解氨酶活性检测试剂盒说明书 BC0215
苯丙氨酸解氨酶（PAL微量法注意：本产品试剂有所变动，请注意并严格按照该说明书操作。
货号：BC0215规格：100T/96S产品组成：使用前请认真核对试剂体积与瓶内体积是否一致，有疑问请及时联系索莱宝工作人员。
试剂名称规格保存条件提取液液体110 mL×1瓶4℃保存试剂一液体15 mL×1瓶4℃保存试剂二粉剂×2瓶4℃保存试剂三液体1 mL×1支4℃保存溶液的配制：1、试剂二：临用前取1瓶加入2.5 mL蒸馏水充分溶解待用；现配现用，4℃可保存2周。
产品说明：PAL（EC4. 3 1 5）广泛存在于各种植物和少数微生物中，是植物体内苯丙烷类代谢的关键酶和限速酶，在动物体内尚未发现。
与一些重要的次生物质如木质素、类黄酮类植保素、黄酮类色素等合成密切相关，在植物正常生长发育、抗病、抗逆反应中起重要作用。
PAL催化L-苯丙氨酸裂解为反式肉桂酸和氨，反式肉桂酸在290nm处有最大吸收值，通过测定吸光值升高速率计算PAL活性。
注意：实验之前建议选择2-3个预期差异大的样本做预实验。
如果样本吸光值不在测量范围内建议稀释或者增加样本量进行检测。
需自备的仪器和用品：紫外分光光度计/酶标仪、水浴锅、台式离心机、可调式移液器、微量石英比色皿/96孔UV板、研钵/匀浆器、冰和蒸馏水。
操作步骤：一、样本处理（可适当调整待测样本量，具体比例可以参考文献）称取约0.1g组织，加入1mL提取液进行冰浴匀浆。
10000g 4℃离心10分钟，取上清，置冰上待测。
二、测定步骤1、分光光度计或酶标仪预热30min以上，调节波长至290nm，蒸馏水调零。
2、操作表：（在EP管或96孔板中按顺序加入下列试剂）试剂名称（μL）测定管空白管样本5试剂一145150试剂二4040BC0215 -- 第1 页，共2 页混匀，30℃准确反应30min试剂三1010混匀，静置10min后，290nm处记录测定管吸光值A1和空白管吸光值A2，ΔA=A1-A2。
普腾免疫试剂二抗应用说明书
IntroductionApplications ForSecondary AntibodiesIn many biological assays, the target antigen is visualizedindirectly. That is, the secondary antibody, bound to the primary antibody, produces the signal. Since many secondary antibodies can bind to one primary antibody, the result is signal amplification and increased sensitivity.The choice of the appropriate secondary antibody is determined by the specifications (i.e. species, subclass, and fragment type) of the primary antibody and the application. Proteintech ®* offers a variety of secondary antibodies suitable for Western Blotting, ELISA, cellular imaging, and flow cytometry: –Enzyme/Biotin conjugated–Conventional fluorescent conjugatedWestern Blotting ELISAImmunofluorescence Staining ImmunohistochemistryFlow CytometrySECONDARY ANTIBODIES*Proteintech and the Proteintech logo are trademarks of Proteintech Group registered in the US Patent and Trademark Office.Secondary AntibodiesSelecting A SuitableSecondary AntibodyFor Your ApplicationUnderstanding Common Secondary Antibody Nomenclature 1. Host Species Of Primary AntibodyThe secondary antibody has to be raised against the host species of the primary antibody (e.g., anti-mouse, rabbit, rat).2. Class Of Primary AntibodyIn addition to the host specificity, the secondary antibody also has to be specific for the isotype of the primary antibody (e.g., IgG, IgA, IgM).4. Assay RelevanceThe intended application determines the type of conjugate (eg., enzyme orfluorescent conjugated).5. Additional Purification StepsA common purification technique for secondary antibodies is High Affinity Purification, resulting in reduced non-specific binding. In addition, secondary antibodies can go through an additional purification step to reduce potential cross-reactions with other species. Therefore, the secondary antibody solution is passed over different columns containing sera proteins of different species to filter out the non-specific secondary antibody (e.g., for multi-stainings).3. Antibody FragmentsIf the primary antibody is not thewhole antibody but a fragment,the secondary antibody shouldalso be fragment-specific to reducenoise signal (e.g., F(ab’)₂ fragmentsecondary antibodies penetratetissue easier in IHC).Ms Mouse Gt Goat rRb Rabbit Sh SheepRt Rat BV Bovine Hn Human Sw SwineIgG (H+L), heavy and light chain IgG3IgG1 IgG, Fc, receptor bindingIgG2a IgAIgG2bIgMUnderstanding the needs of the primary antibody and assay-dependent requirements are essential for selecting an appropriatesecondary antibody.2Seconday Antibodies3 Enzyme Conjugated/ Biotin Secondary AntibodiesConventional Fluorescent ConjugatedSecondary AntibodiesOPTIMIZE YOUR ANTIBODY EXPERIMENTS WITH PROTEINTECHRequest your free technical guide from .LIVE CHAT TWITTER@proteintechBLOG/news/blog YOUTUBE/ProteintechSupportAvailable 24 hours via Live Chat and 9-5 (CDT) via phone.Email support also available.1 (888) 4PTGLAB (1-888-478-4522) (toll free in USA),or 1(312) 455-8498 (outside USA)1 (312) 455-8408Proteintech Group, Inc.5400 Pearl Street, Suite 300, Rosemont, IL 60018, USA **********************PHONEFAX ADDRESSEMAILPHONE EMAIL FAX ADDRESSEMAIL+44 (161) 8393007+44 (161) 2413103Proteintech Europe, Ltd. 4th Floor,196 Deansgate, Manchester, M3 3WF ***********************************Sales and technical support only.PHONE FAX ADDRESSEMAIL************or 4006-900-926************Wuhan Sanying Biotechnologies D3-3, No.666 Gaoxin Avenue,Wuhan East Lake Hi-tech Development Zone Wuhan, Hubei, P .R.C******************We are ISO 9001 and ISO 13485 accredited.Proteintech GroupUS Head OfficeProteintech EuropeUnited KingdomProteintech EuropeGermanyProteintechChina OfficeCONTACT US。
安捷伦产品目录
15
Real-Time PCR
16
Mx3000P QPCR System
17
Brilliant III Ultra-Fast SYBR Green QPCR and QRT-PCR Reagents
18
Brilliant III Ultra-Fast QPCR and QRT-PCR Reagents
Agilent / STRATAGENE
Agilent website: /genomics
Welgene | Agilent Stratagene
威健股份有限公司 | Stratagene 總代理
Table of Content
Table of Contents
/ XL1-Red Competent Cells SoloPack Gold Supercompetent Cells
/ TK Competent Cells Specialty Cells
/ Classic Cells / Fine Chemicals For Competent Cells
適用於 UNG 去汙染或 bisulphite
sequencing
適用於 TA Cloning
最高敏感性
取代傳統 Taq 的好選擇
-
2
威健股份有限公司 | Stratagene 總代理
PCR Enzyme & Instrument
Agilent SureCycler 8800
市場上領先的 cycling 速度和 sample 體積 10 ～ 100 μL 簡易快速可以選擇 96 well 和 384 well 操作盤 優秀的溫控設備讓各個 well 都能保持溫度的穩定 七吋的高解析度觸控螢幕讓操作上更為簡便 可以透過網路遠端操控儀器及監控儀器 Agilent 專業的技術支援可以幫助您應對各種 PCR 的問題
Abcam Bis-Tris 蛋白质凝胶 - RunBlue - 12% 17 孔说明书
Version 1 Last updated 14 January 2020ab270466Bis-Tris Protein Gels - RunBlue™ 12% 17-wellView ab270466Bis-Tris Protein Gels - RunBlue™ - 12% 17-well datasheet: /ab270466(use /ab270466 for China, or www.abcam.co.jp/ab270466 for Japan) This product is for research use only and is not intended for diagnostic use.Table of Contents1.Overview12.Materials Supplied and Storage23.Materials Required, Not Supplied24.General guidelines, precautions, and troubleshooting35.Reagent Preparation46.Sample Preparation57.Assay Procedure68.Notes81.OverviewBis-Tris Protein Gels - RunBlue™ - 12% 17-well (ab270466) precast gels have superior rigidity and stability over traditional polyacrylamide gels. For your convenience we have already removed the comb. The cassette locks the fingers in place and there is no tape or strip to be removed.2.Materials Supplied and StorageStore at +4°C immediately on receipt. Bis-Tris Protein Gels - RunBlue™ can also be stored for 3 months at room temperature.3.Materials Required, Not SuppliedThese materials are not included in the kit, but will be required to successfully perform this assay:-MES or MOPS buffer-Adjustable pipette or multiple-channel pipette4.General guidelines, precautions, andtroubleshootingPlease observe safe laboratory practice and consult the safety datasheet.For general guidelines, precautions, limitations on the use of our assay kits and general assay troubleshooting tips, particularly for first time users, please consult our guide:/assaykitguidelinesFor typical data produced using the assay, please see the assay kit datasheet on our website.Prepare fresh reagents immediately prior to use.Running Buffer Preparation:Reagent Volume (mL) MES Run Buffer - RunBlue™ (ab270224) or50MOPS Run Buffer - RunBlue™ (ab270225)Ultrapure water950Total Volume1000When running reduced samples, we recommend adding Antioxidant (800X) (ab119200) to the 1X SDS Running buffer to provide the best possible resolution of bands.Only use MES Run Buffer - RunBlue™ (ab270224) or MOPS Run Buffer - RunBlue™ (ab270225) running buffers. Other Running Buffer formulas will not produce good results. Ensure buffers are correctly diluted with ultra-pure water if made from stock solutions. We recommend using fresh buffer for each run for both the inner and outer chamber. Never use old buffers for the inner chamber (cathode).6.1We recommend using 4x LDS Sample Buffer – Bis-Tris -RunBlue™ (ab270226) which has been specifically formulated for use with our gels. The ions in the sample buffer match thegel buffer and it has a higher density, making it compatiblewith the density of the running buffer.Reagent Reduced Non-ReducedSample X μL X μLDeionized water 6.5 - X μL7.5 - X μL4x LDS Sample Buffer – Bis-Tris -2.5 μL 2.5 μL RunBlue™ (ab270226)DTT Reducer (10X) (ab119199) 1 μL---Total Volume10 μL10 μL6.2Heat the samples, reduced or non-reduced, for 10 minutes at70°C.6.3Reduced samples should be run within 2 hours to prevent re-oxidation.6.4Maximum volume that can be loaded per well for 17-well gelsis 25 μL.7.Assay ProcedureEquilibrate all materials and prepared reagents to room temperature just prior to use and gently agitate.7.1Sample Loading:7.1.1Shortly before loading the samples, rinse the wells two timeswith ultrapure water. Use thin pipette tips to load samplesnear the bottom of the well.7.2Run Conditions:7.2.1Place the RunBlue TM gel cassette in the tank so that theshorter plate faces the buffer core.7.2.2When running one gel, use a buffer dam to seal the otherside.7.2.3Fill the inner (cathode) chamber with 200 ml fresh runningbuffer up to the top.7.2.4Check whether the cell has been assembled properly so thatthere are no leaks, then pour approximately 600 ml runningbuffer into the outer chamber.7.2.5Do not use less than 400 ml in the outer chamber.7.2.6Run the gel(s) until the blue dye front nears the bottom of thecassette.MES buffer MOPS buffer Voltage200V Voltage200VStart current 90-120 mA/gel Start current 80-115 mA/gelEnd current40-60 mA/gel End current40-60 mA/gelRun time~ 45 min Run time~ 45 min7.3Gel staining:7.3.1Remove the gel from the cassette into a staining tray andcover with 25 mL InstantBlue™ (ab119211). Protein bands willbe visible within minutes. Leave the gel in stain for at leastone hour before transferring into water, if you wish to dry orstore the gel. Alternatively store the gel in stain.7.3.2For silver staining, fix proteins for 10 minutes with a solution of50% methanol, 12% acetic acid and 20mM sodium bisulfite.Substitute this fix step with the manufacturer’s silver stainingprotocol and follow the remaining manufacturer’s method. 7.3.3Other gel stains can be used with RunBlue TM gels, please referto protocols relevant to the specific stain.7.4Gel drying:The gels can be dried without cracking between cellophaneafter equilibrating with gel drying solution.7.4.1Ensure that the gel has been staining for at least 1 hour.Further processing of the gel prior to completion of thestaining process may result in protein destaining and reducedsensitivity. If this occurs simply restain the gel by incubatingovernight in InstantBlue™.7.4.2Submerse the gel in approximately 100 mL ultrapure waterand incubate for at least 1 hour while gently rocking.Optionally adsorbent paper or paper towel can be added.Gels can be incubated overnight in water.7.4.3Incubate the gel in gel drying solution for 10 minutes and wet2 cellophane membranes.7.4.4The gel is now ready for drying between the wettedcellophane membranes.7.5Gel blotting:7.5.1Follow the general guidelines for your blotting unit. Tris-Glycine SDS Blot Buffer - RunBlue™ (ab270227) contains 0.25MTris (base), 1.92M Glycine, and 1% SDS. Dilute the Blot buffer: 7.5.2Equilibrate gels in 1x Blot buffer for 5 to 10 minutes prior toblotting.7.5.3Equilibrate pre-cut Nitrocellulose (NC) or PVDF membranes in1x Blot buffer for 3-5 minutes. (PVDF must be wetted in 100%methanol or ethanol prior to equilibration in buffer.)Note: You can now speed up your transfer to as little as20 minutes using Transfer Buffer - InstantBlot (ab270047) transferbuffer.8.Notesab270466 Bis-Tris Protein Gels - RunBlue™ - 12% 17-well9Technical SupportCopyright © 2020 Abcam. All Rights Reserved. The Abcam logo is a registered trademark. All information / detail is correct at time of going to print.Austria**********************************|019-288-259France*****************************|01.46.94.62.96Germany**********************************|030-896-779-154Spain***************************|91-114-65-60Switzerland*******************Deutsch: 043-501-64-24 | Français: 061-500-05-30UK, EU and ROW*******************|+44(0)1223-696000Canada**********************|877-749-8807US and Latin America**********************|888-772-2226Asia Pacific**********************|(852)2603-6823China**********************|4009210189|+862120700500 Japan******************.jp|+81-(0)3-6231-0940Singapore**********************|800188-5244Australia**********************|+61-(0)3-8652-1450New Zealand********************|+64-(0)9-909-7829Copyright © 2020 Abcam. All rights reserved。
罗米司亭及艾曲波帕药品说明书
根据病人的病情选择：1.确定什么原因引起的血小板减少，至少要找到疾病的诱因。
免疫系统？骨髓造血系统？分泌（激素）？2.若不能找到诱因，一定要使用该两种药品，先用6周，看看效果，查血小板计数，是否继续使用，再做评估。
罗米司亭【商品名】Nplate【药品名称】罗米司亭/ romiplostim【适应症】治疗脾切除和脾未切除慢性免疫性血小板减少性紫癜（ITP）成人患者的血小板生成药。
【用法用量】（1）初始剂量1μg/kg每周1次皮下注射。
（2）因为需要减低出血的风险，通过增量1μg/kg调整每周剂量以达到和维持血小板计数50 × 109/L.（3）最大剂量不要超过每周10μg/kg。
如血小板计数达>400×109/L不要给药。
（4）如在最大剂量4周后血小板计数不增加中断Nplate。
（5）在配制期间不要震荡；避光保护配制好的Nplate； 24小时给配制好的Nplate。
（6）注射容积可能非常小。
使用刻度0.01 mL的注射器。
（7）遗弃单次使用小瓶中未使用部份。
【注意事项】（1）Nplate增加骨髓网硬蛋白(reticulin)沉积的风险；临床研究未除外网硬蛋白和其它纤维沉积导致有血细胞减少的骨髓纤维化的可能性。
监查外周血骨髓纤维化征象。
（2）中止Nplate可能导致血小板减少比Nplate治疗前更坏。
Nplate中止后监查全血细胞计数(CBCs)，包括血小板计数至少2周。
（3）过量Nplate可能增加血小板计数至产生血栓形成/栓塞并发症的水平。
（4）如随Nplate初期反应后血小板计数严重减低评估患者中和抗体的形成。
（5）Nplate可能增加血液学恶性病的风险，尤其是有骨髓增生异常综合征患者。
（6）每周监查CBCs，包括血小板计数和外周血涂片，直至达到稳定的Nplate 剂量。
其后，至少每月监查CBCs，包括血小板计数和外周血涂片。
（7）只能通过受限制的分配计划，称为Nplate NEXUS(了解和支持Nplate专家和患者网络)计划，才能获得Nplate。
IFCC Aspartate Aminotransferase 检测手册说明书
ASTAspartate Aminotransferase IFCCMANUAL RX MONZAINTENDED USEFor the quantitative in vitro determination of AspartateAminotransferase (AST) in serum and plasma. This product is suitable for manual use and on the Rx Monza analyser.Cat. No. AS 1202 R1a. Buffer/Substrate 1 x 70 ml 20 x 2 ml R1b. Enzyme/Coenzyme/ 20 x 2 ml α-oxoglutarate GTIN: 05055273200416AS 1204 R1a. Buffer/Substrate 1 x 105 ml 10 x 10 ml R1b. Enzyme/Coenzyme/ 10 x 10 ml α-oxoglutarate GTIN: 05055273200423AS 1267 R1a. Buffer/Substrate 1 x 105 ml 5 x 20 ml R1b. Enzyme/Coenzyme/ 5 x 20 ml α-oxoglutarate GTIN: 05055273200430AS 2359 R1a. Buffer/Substrate 5 x 100 ml 5 x 100 ml R1b. Enzyme/Coenzyme/ 5 x 100 ml α-oxoglutarate GTIN: 05055273200454UV METHODThis is an optimised standard method according to the concentrations recommended by the IFCC.CLINICAL SIGNIFICANCE (1,2,3,4)The aminotransferases are a group of enzymes that catalyse the inter conversions of amino acids and α-oxoacids by transfer of amino groups. AST (aspartate aminotransferase or glutamate oxaloacetatetransaminase) has been found in the cytoplasm and the mitochondria of cells that have been studied. In cases of mild tissue damage, e.g. liver, the predominant form of serum AST is that from the cytoplasm, with a smaller amount coming from the mitochondria. Severe tissue damage will result in more mitochondrial enzyme being released. Elevated levels of AST can signal myocardial infarction, hepatic disease, muscular dystrophy and organ damage.Although heart muscle is found to have the most activity of the enzyme, significant activity has also been seen in the brain, liver, gastric mucosa, adipose tissue and kidneys of humans.The IFCC has now recommended (1980) standardised procedures for AST determinations including:-1. optimization of substrate concentrations.2. Employment of Tris buffers (instead of phosphate, which has beenshown to inhibit recombination of the apoenzyme with pyridoxal phosphate).3. Pre-incubation of combined buffer and serum to allow sidereactions with NADH to occur. 4. Substrate start (α-oxoglutarate)5. Optional pyridoxal phosphate activation.This is an optimised standard method according to the recommendations of the IFCC.PRINCIPLEα-oxoglutarate reacts with L-aspartate in the presence of AST to form L-glutamate plus oxaloacetate. The indicator reaction utilises the oxaloacetate for a kinetic determination of NADH consumption. AST -oxoglutarate + L-aspartate L-glutamate + oxaloacetate MDH oxaloacetate + NADH + H + L-malate + NAD +SPECIMEN COLLECTION AND PREPARATION (5) Serum:- Use serum free from haemolysis.Plasma:- EDTA or heparin can be used as the anticoagulant.Plasma should be separated from cells within one hour after collection.Specimens should be refrigerated if not used immediately:-Specimens stored longer than 3 days should be frozen at -20︒C.REAGENT COMPOSITIONContents Concentrations in the TestR1a. Buffer/Substrate Tris buffer 80 mmol/l, pH 7.5 L-aspartate 240 mmol/l R1b. Enzyme/Coenzyme/α-oxoglutarate α-oxoglutarate 12 mmol/l MDH ≥420 U/l LD ≥600 U/l NADH 0.18 mmol/lSAFETY PRECAUTIONS AND WARNINGS For in vitro diagnostic use only. Do not pipette by mouth.Exercise the normal precautions required for handling laboratory reagents.Solution R1a contains Sodium Azide. Avoid ingestion or contact with skin or mucous membranes. In case of skin contact, flush affected area with copious amounts of water. In case of contact with eyes or if ingested, seek immediate medical attention.Sodium Azide reacts with lead and copper plumbing, to form potentially explosive azides. When disposing of such reagents flush with large volumes of water to prevent azide build up. Exposed metal surfaces should be cleaned with 10% sodium hydroxide.Health and Safety data sheets available on request.The reagents must be used only for the purpose intended by suitably qualified laboratory personnel, under appropriate laboratory conditions.STABILITY AND PREPARATION OF REAGENTS R1a. Buffer/SubstrateContents ready for use. Stable up to the expiry date when stored at +2 to +8︒C.R1b. Enzyme/Coenzyme/α-oxoglutarate Reconstitute one vial of Enzyme/Coenzyme/α-oxoglutarate R1b with the appropriate volume of Buffer/Substrate R1a: 2 ml for the 20 x 2 ml kit (AS 1202) 10 ml for the 10 x 10 ml kit (AS 1204) 20 ml for the 5 x 20 ml kit (AS 1267) Stable for 14 days at +2 to +8︒C or 24 hours at +15 to +25︒C. Cat. AS 2359 5 x 100 mlReconstitute one vial of Enzyme/Coenzyme/α-oxoglutarate R1b with a portion of Buffer/Substrate R1a and then transfer the entire contents to bottle R1a rinsing bottle R1b several times. Stable for 14 days at +2 to +8︒C or 24 hours at +15 to +25︒C.MATERIALS PROVIDED Buffer/SubstrateEnzyme/Coenzyme/ -oxoglutarateMATERIALS REQUIRED BUT NOT PROVIDEDRandox Assayed Multisera Level 2 (Cat. No. HN 1530) and Level 3 (Cat. No. HE 1532)Randox Calibration Serum Level 3 (Cat. No. CAL 2351) RX series Saline (Cat. No. SA 3854)PROCEDUREAspirate fresh ddH 2O and perform a new Gain Calibration in flow cell mode. Select AST in the Run Test screen and carry out a water blank as instructed.Pipette into a test tube:Sample 0.05 ml Reagent 0.5 mlMix and aspirate into the Rx Monza.CALIBRATION FOR RX MONZAThe use of Saline and Randox Calibration Serum Level 3 isrecommended for calibration. Calibration is recommended with change of reagent lot or as indicated by quality control procedures.FOR MANUAL USEWavelength: 340 nm (Hg 334 nm or Hg 365 nm) Cuvette: 1 cm light path Temperature: 25/30/37︒C Measurement: against airPipette into cuvette: Macro MicroSample 0.2 ml 0.1 ml Enzyme/Coenzyme/ α-oxoglutarate R1 2.0 ml 1.0 mlMix, read initial absorbance after 1 minute. Read again after 1, 2 and 3 minutes. Note: If the absorbance change per minute is between 0.11 and 0.16 at 340/Hg 334 nm 0.06 and 0.08 at Hg 365 nmuse only the values for the first 2 minutes for the calculation.MANUAL CALCULATIONTo calculate the AST activity, use the following formulae:U/l = 1746 x A 340 nm/min U/l = 1780 x A Hg 334 nm/min U/l = 3235 x A Hg 365 nm/minSTANDARDISATIONRandox Calibration Serum Level 3 is traceable to AST reference material JSCC TS01.QUALITY CONTROLRandox Assayed Multisera, Level 2 and Level 3 are recommended for daily quality control. Two levels of controls should be assayed at least once a day. Values obtained should fall within a specified range. If these values fall outside the range and repetition excludes error the following steps should be taken:1. Check instrument settings and light source.2. Check cleanliness of all equipment in use.3. Check water. Contaminants, i.e. bacterial growth, maycontribute to inaccurate results. 4. Check reaction temperature.5. Check expiry date of kit and contents.6. Contact Randox Laboratories Customer Technical Services, Northern Ireland +44 (0) 28 9445 1070.SPECIFICITY/INTERFERENCE (6,7)Gross haemolysis will produce falsely elevated test results. The effects of various drugs on AST activity should be taken intoconsideration in the case of patients receiving large doses of drugs.The analytes below were tested up to the following levels and were found not to interfere: Haemoglobin 250 mg/dl Free Bilirubin 25 mg/dl Conjugate Bilirubin 25 mg/dl Triglycerides 1000 mg/dlIntralipid ® 200 mg/dlA list of substances and conditions known to effect AST activity in vivo is given by both Young et al and Friedman et al. Norepresentation is made by Randox Laboratories Ltd regarding the completeness of these lists and the accuracy of the information contained therein.NORMAL VALUES IN SERUM (8,9) +25︒C +30︒C +37︒C Men up to 18 U/l up to 25 U/l up to 37 U/l Women up to 15 U/l up to 21 U/l up to 31 U/lIt is recommended that each laboratory establish its own reference range to reflect the age, sex, diet and geographical location of the population.SPECIFIC PERFORMANCE CHARACTERISTICS The following performance data were obtained using an Rx Monza analyser running at +37o C.LINEARITYThis method is linear up to 562 U/l. If the sample concentration exceeds this value, dilute the sample 1+9 with 0.9% NaCl solution and re-assay. Multiply the result by 10.SENSITIVITYThe minimum detectable concentration of AST with an acceptable level of precision was determined as 9.3 U/l.PRECISIONIntra AssayLevel 2 Level 3Mean (U/l) 35.6 153SD 1.66 1.47CV(%) 4.65 0.96n 20 20Inter AssayLevel 2 Level 3Mean (U/l) 35.6 153SD 1.77 7.10CV(%) 4.96 4.63n 20 20CORRELATIONThis method (Y) was compared with another commerciallyavailable method (X) and the following linear regression equationobtained:Y = 1.07X + 4.9and a correlation coefficient of r = 0.997543 patient samples were analysed spanning the range 28 to 559U/l.REFERENCES1. Wroblewski F, La Due J.S: Ann Intern Med. 1956; 45: 801.2. Wroblewski F, La Due J.S: Proc Soc Exp Biol Med 1956;91: 569.3. Bergmeyer HU, Bowers GN Jr, et al: Clin Chem 1977; 23:887.4. Bergmeyer HU, Bowers GN Jr, et al: J.Clin Chem ClinBiochem 1980; 18: 521-534.5. Tietz N W: Fundamentals of Clinical Chemistry ed 3.Philadelphia, WB Saunders Co. 1987, pg 372.6. Young D S, et al: Clin Chem 1975, 21; No5.7. Friedman RB, et al: Clin Chem 1980, 26; No4.8. Wallnofer H, Schmidt.E, Schmidt FW, eds: Synopsis derLeberkrankheiten Stuttgart, Georg Thieme Verlag, 1974.9. Thefeld W, et al: Dtsch Med Wschr 1974; 99: 343.Revised 26 Apr 16 biRev. 003THIS PAGE IS INTENTIONALLY BLANK。
AZD7545_SDS_MedChemExpress
Inhibitors, Agonists, Screening LibrariesSafety Data Sheet Revision Date:May-24-2017Print Date:May-24-20171. PRODUCT AND COMPANY IDENTIFICATION1.1 Product identifierProduct name :AZD7545Catalog No. :HY-16082CAS No. :252017-04-21.2 Relevant identified uses of the substance or mixture and uses advised againstIdentified uses :Laboratory chemicals, manufacture of substances.1.3 Details of the supplier of the safety data sheetCompany:MedChemExpress USATel:609-228-6898Fax:609-228-5909E-mail:sales@1.4 Emergency telephone numberEmergency Phone #:609-228-68982. HAZARDS IDENTIFICATION2.1 Classification of the substance or mixtureGHS Classification in accordance with 29 CFR 1910 (OSHA HCS)Acute toxicity, Oral (Category 4),H302Acute aquatic toxicity (Category 1),H400Chronic aquatic toxicity (Category 1),H4102.2 GHS Label elements, including precautionary statementsPictogramSignal word WarningHazard statement(s)H302 Harmful if swallowed.H410 Very toxic to aquatic life with long lasting effects.Precautionary statement(s)P264 Wash skin thoroughly after handling.P270 Do not eat, drink or smoke when using this product.P273 Avoid release to the environment.P301 + P312 IF SWALLOWED: Call a POISON CENTER or doctor/ physician if you feel unwell.P330 Rinse mouth.P391 Collect spillage.P501 Dispose of contents/ container to an approved waste disposal plant.2.3 Other hazardsNone.3. COMPOSITION/INFORMATION ON INGREDIENTS3.1 SubstancesSynonyms:AZD 7545; AZD⁻7545Formula:C19H18ClF3N2O5SMolecular Weight:478.87CAS No. :252017-04-24. FIRST AID MEASURES4.1 Description of first aid measuresEye contactRemove any contact lenses, locate eye-wash station, and flush eyes immediately with large amounts of water. Separate eyelids with fingers to ensure adequate flushing. Promptly call a physician.Skin contactRinse skin thoroughly with large amounts of water. Remove contaminated clothing and shoes and call a physician.InhalationImmediately relocate self or casualty to fresh air. If breathing is difficult, give cardiopulmonary resuscitation (CPR). Avoid mouth-to-mouth resuscitation.IngestionWash out mouth with water; Do NOT induce vomiting; call a physician.4.2 Most important symptoms and effects, both acute and delayedThe most important known symptoms and effects are described in the labelling (see section 2.2).4.3 Indication of any immediate medical attention and special treatment neededTreat symptomatically.5. FIRE FIGHTING MEASURES5.1 Extinguishing mediaSuitable extinguishing mediaUse water spray, dry chemical, foam, and carbon dioxide fire extinguisher.5.2 Special hazards arising from the substance or mixtureDuring combustion, may emit irritant fumes.5.3 Advice for firefightersWear self-contained breathing apparatus and protective clothing.6. ACCIDENTAL RELEASE MEASURES6.1 Personal precautions, protective equipment and emergency proceduresUse full personal protective equipment. Avoid breathing vapors, mist, dust or gas. Ensure adequate ventilation. Evacuate personnel to safe areas.Refer to protective measures listed in sections 8.6.2 Environmental precautionsTry to prevent further leakage or spillage. Keep the product away from drains or water courses.6.3 Methods and materials for containment and cleaning upAbsorb solutions with finely-powdered liquid-binding material (diatomite, universal binders); Decontaminate surfaces and equipment by scrubbing with alcohol; Dispose of contaminated material according to Section 13.7. HANDLING AND STORAGE7.1 Precautions for safe handlingAvoid inhalation, contact with eyes and skin. Avoid dust and aerosol formation. Use only in areas with appropriate exhaust ventilation.7.2 Conditions for safe storage, including any incompatibilitiesKeep container tightly sealed in cool, well-ventilated area. Keep away from direct sunlight and sources of ignition.Recommended storage temperature:Powder-20°C 3 years4°C 2 yearsIn solvent-80°C 6 months-20°C 1 monthShipping at room temperature if less than 2 weeks.7.3 Specific end use(s)No data available.8. EXPOSURE CONTROLS/PERSONAL PROTECTION8.1 Control parametersComponents with workplace control parametersThis product contains no substances with occupational exposure limit values.8.2 Exposure controlsEngineering controlsEnsure adequate ventilation. Provide accessible safety shower and eye wash station.Personal protective equipmentEye protection Safety goggles with side-shields.Hand protection Protective gloves.Skin and body protection Impervious clothing.Respiratory protection Suitable respirator.Environmental exposure controls Keep the product away from drains, water courses or the soil. Cleanspillages in a safe way as soon as possible.9. PHYSICAL AND CHEMICAL PROPERTIES9.1 Information on basic physical and chemical propertiesAppearance White to off-white (Solid)Odor No data availableOdor threshold No data availablepH No data availableMelting/freezing point No data availableBoiling point/range No data availableFlash point No data availableEvaporation rate No data availableFlammability (solid, gas)No data availableUpper/lower flammability or explosive limits No data availableVapor pressure No data availableVapor density No data availableRelative density No data availableWater Solubility No data availablePartition coefficient No data availableAuto-ignition temperature No data availableDecomposition temperature No data availableViscosity No data availableExplosive properties No data availableOxidizing properties No data available9.2 Other safety informationNo data available.10. STABILITY AND REACTIVITY10.1 ReactivityNo data available.10.2 Chemical stabilityStable under recommended storage conditions.10.3 Possibility of hazardous reactionsNo data available.10.4 Conditions to avoidNo data available.10.5 Incompatible materialsStrong acids/alkalis, strong oxidising/reducing agents.10.6 Hazardous decomposition productsUnder fire conditions, may decompose and emit toxic fumes.Other decomposition products - no data available.11.TOXICOLOGICAL INFORMATION11.1 Information on toxicological effectsAcute toxicityClassified based on available data. For more details, see section 2Skin corrosion/irritationClassified based on available data. For more details, see section 2Serious eye damage/irritationClassified based on available data. For more details, see section 2Respiratory or skin sensitizationClassified based on available data. For more details, see section 2Germ cell mutagenicityClassified based on available data. For more details, see section 2CarcinogenicityIARC: No component of this product present at a level equal to or greater than 0.1% is identified as probable, possible or confirmed human carcinogen by IARC.ACGIH: No component of this product present at a level equal to or greater than 0.1% is identified as a potential or confirmed carcinogen by ACGIH.NTP: No component of this product present at a level equal to or greater than 0.1% is identified as a anticipated or confirmed carcinogen by NTP.OSHA: No component of this product present at a level equal to or greater than 0.1% is identified as a potential or confirmed carcinogen by OSHA.Reproductive toxicityClassified based on available data. For more details, see section 2Specific target organ toxicity - single exposureClassified based on available data. For more details, see section 2Specific target organ toxicity - repeated exposureClassified based on available data. For more details, see section 2Aspiration hazardClassified based on available data. For more details, see section 212. ECOLOGICAL INFORMATION12.1 ToxicityNo data available.12.2 Persistence and degradabilityNo data available.12.3 Bioaccumlative potentialNo data available.12.4 Mobility in soilNo data available.12.5 Results of PBT and vPvB assessmentPBT/vPvB assessment unavailable as chemical safety assessment not required or not conducted.12.6 Other adverse effectsNo data available.13. DISPOSAL CONSIDERATIONS13.1 Waste treatment methodsProductDispose substance in accordance with prevailing country, federal, state and local regulations.Contaminated packagingConduct recycling or disposal in accordance with prevailing country, federal, state and local regulations.14. TRANSPORT INFORMATIONDOT (US)This substance is considered to be non-hazardous for transport.IMDGUN number: 3077Class: 9Packing group: IIIEMS-No: F-A, S-FProper shipping name: ENVIRONMENTALLY HAZARDOUS SUBSTANCE, SOLID, N.O.S.Marine pollutant: Marine pollutantIATAUN number: 3077Class: 9Packing group: IIIProper shipping name: Environmentally hazardous substance, solid, n.o.s.15. REGULATORY INFORMATIONSARA 302 Components:No chemicals in this material are subject to the reporting requirements of SARA Title III, Section 302.SARA 313 Components:This material does not contain any chemical components with known CAS numbers that exceed the threshold (De Minimis) reporting levels established by SARA Title III, Section 313.SARA 311/312 Hazards:No SARA Hazards.Massachusetts Right To Know Components:No components are subject to the Massachusetts Right to Know Act.Pennsylvania Right To Know Components:No components are subject to the Pennsylvania Right to Know Act.New Jersey Right To Know Components:No components are subject to the New Jersey Right to Know Act.California Prop. 65 Components:This product does not contain any chemicals known to State of California to cause cancer, birth defects, or anyother reproductive harm.16. OTHER INFORMATIONCopyright 2017 MedChemExpress. The above information is correct to the best of our present knowledge but does not purport to be all inclusive and should be used only as a guide. The product is for research use only and for experienced personnel. It must only be handled by suitably qualified experienced scientists in appropriately equipped and authorized facilities. The burden of safe use of this material rests entirely with the user. MedChemExpress disclaims all liability for any damage resulting from handling or from contact with this product.Caution: Product has not been fully validated for medical applications. For research use only.Tel: 609-228-6898 Fax: 609-228-5909 E-mail: tech@Address: 1 Deer Park Dr, Suite Q, Monmouth Junction, NJ 08852, USA。
Anti-HiBiT Monoclonal Antibody n7200 N7210 使用说明书
2023版 CTM719原英文技术手册TM719中 文 说 明 书适用产品目录号：N7200、N7210Anti-HiBiT Monoclonal Antibody普洛麦格(北京)生物技术有限公司Promega (Beijing) Biotech Co., Ltd 地址：北京市东城区北三环东路36号环球贸易中心B座907-909电话：************网址：技术支持电话：400 810 8133技术支持邮箱：*************************CTM7192023制作1所有技术文献的英文原版均可在/ protocols 获得。
请访问该网址以确定您使用的说明书是否为最新版本。
如果您在使用该试剂盒时有任何问题，请与Promega 北京技术服务部联系。
电子邮箱：*************************1. 描述 (2)2. 产品组分和储存条件 (3)3. 免疫印迹分析 (3)3. A. 一般注意事项 (3)3. B. 示例操作流程 (4)3. C. 示例数据 (5)4. 免疫荧光分析 (7)4. A. 一般注意事项 (7)4. B. 示例操作流程 (8)4. C. 示例数据 (9)5. 免疫沉淀 (10)5. A. 一般注意事项 (10)5. B. 示例操作流程 (11)5. C. 示例数据 (12)6. 用活细胞进行荧光激活细胞分选（FACS） (15)6. A. 一般注意事项 (15)6. B. 示例操作流程 (15)6. C. 示例数据 (17)7. 用固定细胞进行荧光激活细胞分选 (17)7. A. 一般注意事项 (17)7. B. 示例操作流程 (18)7. C. 示例数据 (19)8. 抗体结合亲和力和与LgBiT的竞争 (19)9. 疑难排查 (21)9. A. 免疫印迹分析 (21)9. B. 免疫荧光分析 (22)9. C. 免疫沉淀 (22)9. D. 荧光激活细胞分选 (23)10.参考文献 (24)11.相关产品 (24)Anti-HiBiT Monoclonal Antibody普洛麦格(北京)生物技术有限公司Promega (Beijing) Biotech Co., Ltd 地址：北京市东城区北三环东路36号环球贸易中心B座907-909电话：************网址：技术支持电话：400 810 8133技术支持邮箱：*************************CTM7192023制作21.描述HiBiT蛋白标签为含有11个氨基酸的多肽，在NanoLuc® Binary Technology（NanoBiT®）中，可与大亚基（LgBiT）以高亲和力结合，以重建NanoBiT®萤光素酶，一种明亮的发光酶（1,2）。
Atto 488 Protein Labeling Kit产品说明书
38371 Atto 488 Protein Labeling KitIntroductionThe Atto 488 Protein Labeling Kit provides a fast labeling of purified proteins as well as an easy purification of the resulting conjugate. The succinimidyl ester group of the dye enables an effective reaction with primary amino groups of the protein. The kit contains everything for 5 labelings including purification procedures. It is optimized for 1 mg protein per reaction. A final molar dye / protein (D/P) ratio between 2 – 9 can be expected, depending on the kind of protein 1).Kit componentsA) 5 vials Atto 488 reactive dye, each containing 0.24 mg dye (optimized for 1 mg protein)product no. 41698B) Sodium bicarbonate buffer solution, pH 9.5, 10 ml, product no. 88975C) Phosphate buffer solution, pH 7.5, 100 ml, product no. 76847D) 5 gel filtration columns (PD-10), product no. 73226E) Application noteStorageStore the kit at 4 °C, protected from moisture. Protect the reactive dye from light.Label characteristicsAtto 488 NHS ester (product no. 41698)λabs max = 500 nm (in 0.1 M phosphate buffer solution pH 7.0)λem max = 520 nm (in 0.1 M phosphate buffer solution pH 7.0)ε = 90 000 cm-1M-1The vibrant M and Sigma-Aldrich are trademarks of Merck KGaA, Darmstadt, Germany or its affiliates.Detailed information on trademarks is available via publicly accessible resources.© 2018 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.Labeling protocol1. Dissolve 2 – 10 mg/ml protein in sodium bicarbonate buffer solution (B). Protein concentration< 2 mg/ml will decrease the labeling efficiency of the labeling reaction. The protein solution has to be free of amino group-containing substances (e.g. Tris buffer solution or ammonium ions). Azide with concentration < 3 mM will not affect the labeling efficiency.2. Dissolve the reactive dye (A) in 20 µl sodium bicarbonate buffer (B). Proceed quickly with thelabeling procedure, so that there is no chance for hydrolysis before coupling to the protein.3. Transfer the protein solution to the vial containing the reactive dye.4. Incubate the reaction mixture for 2 hours while gently stirring and protecting the vial fromlight. It is recommended to prepare the dye solution immediately before starting the coupling reaction. Due to the different reactivity of proteins, the degree of labeling might vary between 2 – 9. The incubation time can be prolonged up to 12 h at 4 °C.Separation of protein-dye-conjugates from free dyeThe gel filtration columns are used in order to achieve good separation of the protein-dye-conjugates from excess free dye (size exclusion limit: 5 kDa). Solvent flow in the columns is achieved by gravity only, there’s no need to apply pressure.1. Remove the top cap and pour off excess liquid (contains 0.15 % Kathon TM 2); take care forsafety issues, wear gloves)2. Remove the bottom cap, then cut off the bottom tip of the column (D)3. Equilibrate the column first with 10 ml water, then with 15 ml phosphate buffer (C). Discard theflow-through.4. Add the labeling mixture to the column and allow to infiltrate5. Add 5ml phosphate buffer (C) and start the elution6. Separation between a first band (dye-labeled protein) and a second band (free dye) willbecome visible7. Collect the dye-labeled protein fraction (= first band)Determination of dye/protein (D/P) ratioAfter a successful separation of the free dye from the labeled protein, the dye to protein ratio (D/P) of the conjugate can be calculated:Measure the absorbance of the conjugates at 280 nm and 498 nm. Before measuring, the conjugate should be diluted so that the maximum absorbance measured is between 0.5 and 1.0 A.U.The D/P molar ratio can be calculated as follows:[]000,90)10.0(/498280Pr 498××−×=A A A P D otein εA 498 = absorbance at 498 nm measured in a cuvette with a path length of 1 cmA 280 = absorbance at 280 nm measured in a cuvette with a path length of 1 cmεProtein = molar extinction coefficient of the protein at 280 nm [cm -1M -1]. IgG usually have ε of 203,000. 90,000 = molar extinction coefficient (εdye ) of the Atto 488 dye at 498 nm [cm -1M -1]0.10 = correction factor due to the fluorophore’s absorbance at 280 nmThe vibrant M and Sigma-Aldrich are trademarks of Merck KGaA, Darmstadt, Germany or its affiliates.Detailed information on trademarks is available via publicly accessible resources.© 2018 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.The final protein concentration can be calculated as follows:factor Dilution MW A A ml mg c otein otein otein ×××−=Pr Pr 498280Pr )10.0()/(εMW Protein = Molecular weight of the protein (for IgG 150,000 g / mol)Dilution factor = Dilution of the labeled conjugate prior to measurement by spectrophotometerHandling of labeled conjugatesLabeled conjugates should be stored at 4 °C and protected from light. To improve stability, sodium azide (0.01 %) may be added. Depending on the type of protein, the conjugates can be stable for several months. For extended storage, the solution should be kept at –20 °C. Avoid repeated freeze-thaw cycles. In order to prevent denaturation of the conjugates add bovine serum albumin (BSA) to a final concentration of 1 – 10 mg / ml or use one of our BioStab stabilizers.ReferencesE. Harlow, D. Lane, Using Antibodies, A Laboratory Manual , Cold Spring Harbor Laboratory Press, New York, 1999Kathon is a registered trademark of Rohm and Haas Co.Precautions and Disclaimer:This product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.。
CoraLite488-Phalloidin(绿色) 产品说明书
Proteintech Group,USA, 5400Pearl Street,Suite300, Rosemont,IL60018,USA t1-888-478-4522Proteintech Europe,4th Floor,196Deansgate,Manchester,M33WFt(+44)-161-83-93-007San Ying Biotechnology,China,D3-3,No.666Gaoxin Avenue,Wuhan East LakeHi-tech Development Zone,Wuhan,P.R.C.t86-27-87531629SAFETY DATA SHEET(SDS)1.IDENTIFICATION OF THE SUBSTANCE/PREPARATION AND OF THECOMPANY/UNDERTAKINGProduct Name:CoraLite®488-Phalloidin(green)Catalog Number:PF00001REACH registration number:No registration number is given yet for this substance/substances in this mixture since the annual import quantity is less than one tonnage per annum or the transition period for its registration according to Article23of REACH has not yet expired.Company/undertaking Identification:Proteintech Group5400Pearl StreetSTE300Rosemont,IL60018312-455-8498Emergency telephone number:312-455-8498,ext8022.HAZARDOUS IDENTIFICATION:Classification according to Regulation(EC)No1272/2008[CLP]Physical hazards:Not hazardousHealth hazards:Not HazardousEnvironmental Hazards:Not HazardousAdditional information:Not applicableLabel elementsLabelling according to Regulation(EC)No1272/2008[CLP]Hazard pictograms:Signal word:DangerHazard statements:Harmful if swallowedEU Specific Hazard Statements:Not applicablePrecautionary StatementsPrevention:Not applicableResponse:Not applicableStorage:Not applicableDisposal:Not applicableOther Hazards:Not applicable3.INGREDIENT COMPOSITION/INFORMATION:Component Cas No.Weight Classification according to Regulation ECNo1272/2008(CLP)Phalloidin None100%Acute tox2–H300,H310,H3304.FIRST AID MEASURES:Skin Contact Wash off skin thoroughly with soap and water.Remove contaminated clothing and wash before reuse.Eye Contact Ensure adequate flushing of eye contamination for at least 15minutes.Inhalation Remove from exposure,rest and keep warm.Ingestion Wash mouth out thoroughly with water and drink plenty of water.Most important symptoms and effects:both acute and delayed Symptoms of allergic reaction may include rash,itching,swelling,trouble breathing,tingling of the hands and feet,dizziness,lightheadedness,chest pain,muscle pain,or flushing.Indication of any immediate medical attention and special treatment needed: Treat symptomatically.Advice for emergency responders:For further assistance,contact your local Poison Control Center.Protection of first-aid responders:Ensure that medical personnel are aware of the material(s)involved,and take precautions to protect themselves.5.FIRE FIGHTING MEASURES:Suitable extinguishing media:Water Spray,Carbon Dioxide,DryChemical Powder,or appropriate foam.Unsuitable extinguishing media:No information availableSpecial hazards arising from the substance or mixture:Not known.Advice for fire-fighters:Standard procedure for chemical fires6.ACCIDENTAL RELEASE MEASURES:Wear appropriate personal protective clothing(Section8).Mop up with an absorbent cloth and arrange removal by a disposal company.Wash site of spillage thoroughly with water and detergent and prevent further leakage or spillage if safe to do so.No special environmental precautions required.7.HANDLING AND STORAGE:Use only in area provided with exhaust ventilation.Avoid contact with skin,eyes and clothing.Wear appropriate protective clothing(Section8).Keep container tightly closed,and in a dark,well-ventilated area.Store at-20℃.8.EXPOSURE CONTROLS/PPE:Follow good laboratory practice when handling this product.Hygiene measures:Handle in accordance with good industrial hygiene and safety practiceRecommended personal protection equipmentEye protection:Laboratory safety goggles.Hands:Chemical resistant gloves.Skin:Laboratory protective clothing.Respiratory:Fume hood or in areas with adequate ventilation.Control Parameters:Not applicable9.PHYSICAL AND CHEMICAL PROPERTIES: Appearance:SolidOdor:NoneMelting point/melting range:UntestedBoiling point/boiling range:UntestedProteintech Group,USA, 5400Pearl Street,Suite300, Rosemont,IL60018,USA t1-888-478-4522Proteintech Europe,4th Floor,196Deansgate,Manchester,M33WFt(+44)-161-83-93-007San Ying Biotechnology,China,D3-3,No.666Gaoxin Avenue,Wuhan East LakeHi-tech Development Zone,Wuhan,P.R.C.t86-27-87531629Flash point:UntestedAutoignition temperature:UntestedDecomposition temperature:UntestedEvaporation rate:UntestedFlammability:UntestedUpper explosion limit:UntestedLower explosion limit:UntestedVapor pressure:UntestedRelative density:UntestedSpecific gravity:UntestedSolubility:UntestedPartition coefficient:UntestedExplosive properties:Untested10.CHEMICAL STABILITY AND REACTIVITY:Reactivity:None knownChemical stability:Stable under normal conditionsPossibility of hazardous reactions:None reportedConditions to avoid:No information availableIncompatible materials:No dangerous reaction knownHazardous decomposition products:None11.TOXICOLOGICAL INFORMATION:Inhalation:May be irritating to mucous membranes and upper respiratory tract.Eye contact:May cause irritationSkin contact:May cause irritationIngestion:May be harmful if swallowedCarcinogenic effects:Not availableMutagenic effects:Not availableReproductive toxicity:Not availableSTOT–Single exposure:Not availableSTOT–Repeated exposure:Not availableTeratogenic effects:Not available12.ECOLOGICAL INFORMATION:Data not yet available.13.DISPOSAL CONSIDERATIONS:Waste disposal methods:Should not be released into the environment.Disposal considerations:Do not empty into drains;dispose of material and container in safe way.14.TRANSPORT INFORMATION:DOTUN number UN2811UN proper shipping name Toxic solid,organic,n.o.s.(phalloidin)Transport hazard class(es)6.1Packing group II Special provisions IB8,IP2,IP4,T3,TP33Emergency response guide154numberIATAUN number UN2811UN proper shipping name Toxic solid,organic,n.o.s.(phalloidin)Transport hazard class(es)6.1Packing group IISpecial provisions A3,A5.15.REGULATORY INFORMATION:Substances of Very High Concern:None.Restricted substances under EC1907/2006,Annex XVII:None. Substances listed under Annex I of Regulation(EC)No689/2008:None. Restricted substances under Annex V of Regulation(EC)No689/2008:None. Substances under Regulation(EC)No850/2004of the European Parliament and of the Council of29April2004on persistent organic pollutants and amending Directive79/117/EEC:None.German Water hazard classes(Wassergefährdungsklassen):Not classified. Other International Inventories:No information available16.OTHER INFORMATION:Reason for revision:New productRevision number:1Revision:2/4/2021The above information is believed to be correct but should only be used as a guide for experienced personnel.Proteintech Group Inc.will not be liable for any damage resulting from the handling of or from contact with the above product.For lab use only,not for diagnostic or therapeutic work.。
N6-isopentenyladenosine_AbMole中国_CAS号7724-76-7_M9450说明书Protocol
狗
重量 (kg) 体表面积 (m2)
0.02 0.007
0.15 0.025
1.8
0.4
0.08
10
0.15
0.05
0.02
0.5
Km 系数
3
6
12
8
5
20
动物 A (mg/kg) = 动物 B (mg/kg) × 动物 B的Km系数 动物 A的Km系数
Concentration / Solvent Volume / Mass
1 mg
5 mg
10 mg
1 mM
2.9819 mL
14.9094 mL
29.8187 mL
5 mM
0.5964 mL
2.9819 mL
5.9637 mL
10 mM
0.2982 mL
1.4909 mL
2.9819 mL
所有产品仅供科研使用，严禁用于人或动物的治疗等任何其他用途，不为任何个人提供产品和服务。 抑制剂 化合物库 CCK-8 / 蛋白酶抑制剂Cocktail
例如，依据体表面积折算法，将白藜芦醇用于小鼠的剂量22.4 mg/kg 换算成大鼠的剂量，需要将22.4 mg/kg 乘以小鼠的Km系数（3）， 再除以大鼠的Km系数（6），得到白藜芦醇用于大鼠的等效剂量为11.2 mg/kg。
储备液配制 以下数据基于产品分子量，对于特殊产品，请参照COA中的储备液配制条件和说明进行操作。
AbMole中国
产品说明 Protocol
N6-isopentenyladenosine 目录号M9450
化学数据
分子量
335.36