斑节对虾真核生物翻译起始因子3亚基4(btseIF3g)序列(精)

All Rights Reserved. South China Fisheries Science

第5卷第3期2009年6月

南　方　水　产

South China Fisheries Science

Vol 15,No 13

Jun 1,2009

doi:1013969/j 1issn 11673-2227120091031001收稿日期:2009203216;修回日期:2009203224

资助项目:国家自然科学基金项目(30571447);国家高技术研究发展计划(863计划)项目(2006AA603120);“十一五”国家科技支撑计划项目(2007BAD29B0129);广东省自然科学基金项目(7003894)

作者简介:陈　亮(1982-),男,硕士研究生,从事水产经济动物繁殖生物学研究。E 2mail:cl820619@1631com 通讯作者:江世贵,E 2mail:jiangsg@21cn 1com

斑节对虾真核生物翻译起始因子3亚基

4(btseI F3g )

序列分析及组织表达特性

陈　亮1,2,张殿昌1,杨丽诗1,黄建华1,杨其彬1,江世贵1

(11中国水产科学研究院南海水产研究所,广东广州510300;21广东海洋大学水产学院,广东湛江524088)摘要:通过mRNA 差异显示(differential dis p lay,DD )技术筛选的差异片段,与斑节对虾(Penaeus m onodon )

混合组织

c DNA 文库中的EST 进行拼接,获得斑节对虾真核生物翻译起始因子3亚基4(btse I F3g )的全序列(Gen Bank 登录号:FJ829364)。btse I F3g 全长1041bp,由72bp 5′非编码区、90bp 3′非编码区以及879bp 的开放阅读框组成,推测编码一个长度为292个氨基酸的蛋白。经BLAST 分析,btse I F3g C 端含有一个RNA 识别基序(RNA recogniti on motif,RR M )的保守结构域(E 值为2172e 225)。利用氨基酸序列进行系统发育分析表明,btse I F3g 与e I F3g 属于同一分支。采用荧光定量PCR (Real 2ti m e PCR )方法对250日龄的雌、雄斑节对虾不同组织表达特性研究发现,btse I F3g 在眼柄神经节、脑神经、肝胰腺、性腺和肌肉组织中均有表达,在卵巢中的相对表达量极显著

(P <0101)高于其他组织,这一结果提示e I F3g 可能与斑节对虾卵巢发育相关。关键词:斑节对虾;e I F3g;克隆;组织表达中图分类号:Q785文献标识码:A 文章编号:1673-2227-(2009)03-0001-09Sequence analysis of eukaryoti

c transl ati on i n iti a ti on factor 3subun it G (eI F 3g)of bl ack ti ger shr imp (Penaeus m onodon )an

d its mRNA tissu

e expressi on CHEN L iang 1,2,Z HANG D ianchang 1,Y ANG L ishi 1,HUANG J ianhua 1,Y ANG Q ibin 1,J I A NG Shigui

1

(1.South China Sea F isheries R esearch Institute,Chinese A cade m y of Fishery Sciences,Guangzhou 510300,China ;

21Fisheries College of Guangdong O cean U niversity,Zhanjiang 524088,China )Abstract :I n order t o analyze the functi on of a differential exp ressi on c DNA frag ment of gonads of domesticated br oodst ock black tiger

shri m p (Penaeus m onodon ),the black tiger shri m p eukaryotic translati on initiati on fact or 3subunit G (btse I F3g )had been f ound by

s p liced with the EST,after screening in the P 1m onodon m ix c DNA library 1The full length cDNA of btse I F3g (Gen Bank accessi on no 1

FJ829364)contained a 5′untranslated regi on (UT R )of 72bp,a 3′UTR of 90bp and an ORF of 879bp encoding a polypep tide of

292a m ino acids with an esti m ated molecular mass of 32197k Da 1RNA recogniti on motif (RR M )was f ound in the btse I F3g deduced a 2

m ino acid sequence 1Phyl ogenetic analysis of the e I F3gene fa m ily indicated that the btse I F3g was homol ogous t o the e I F3g 1mRNA ex 2

p ressi on of btse I F3g could be detected in 2502day 2old P 1m onodon ′s eyestalk,brain,hepat opancreas,gonad and muscle by fluorescent

quantitative real 2ti m e PCR analysis 1The highest exp ressi on level of btse I F3g in the ovary indicated that btse I F3g p layed an i m portant

r ole in oogenesis 1

Key words :Penaeus m onodon ;e I F3g;molecular cl oning;tissue exp ressi on