艾叶提取工艺及抗氧化活性的研究(1)

华东理工大学学报(自然科学版)

Journal of East Ch ina U niversity of Science and T echno logy (N atural Science Editi on )

V o l .31N o.62005212

基金项目:上海市科委纳米专项项目(0452nm 009)资助

E -ma il :m inbo lan@ecust .edu .cn

收稿日期:2004212210

作者简介:袁慧慧(19732),女,助理研究员。

文章编号:100623080(2005)0620768204

艾叶提取工艺及抗氧化活性的研究

袁慧慧1,　殷日祥2,　陆冬英2,　蓝闽波1,2,33

(1.华东理工大学超细材料制备与应用教育部重点实验室,上海200237;

2.华东理工大学分析与测试中心;

3.华东理工大学结构可控先进功能材料及其制备教育部重点实验室)

摘要:通过正交实验方法研究了艾叶醇2水体系的提取工艺,采用比色法测定其黄酮类和多糖类化合物的含量,得到不同因素对总黄酮和总多糖提取的影响程度依次为:乙醇浓度>提取温度>提取时间>料液比;并以化学发光法测定各提取液的抗氧化活性。结果表明;艾叶中含有丰富的黄酮类、多糖类化合物,具有很强的抗氧化活性。

关键词:艾叶;抗氧化活性;总黄酮;总多糖;化学发光

中图分类号:TQ 284.2

文献标识码:A

Study on Extracti ng Processi ng of Foliu m A rtem isiae a rgyi

and An ti -ox idan t Activ ity

YUA N H u i 2hu i 1

,　Y IN R i 2x iang 2

,　L U D ong 2y ing 2

,　L A N M in 2bo

1,2,33

(1.K ey L abora tory f or U ltraf ine M a teria ls of M in istry of E d uca tion ,E ast Ch ina U n iversity

of S cience and T echnology ,S hang ha i 200237,Ch ina ;2.A na ly sis and R esea rch Cen ter ,E ast Ch ina U n iversity of S cience and T echnology ;3.K ey L abora tory f or A d vanced M a teria ls of M in istry of E d uca tion ,E ast Ch ina U n iversity of S cience and T echnology )

Abstract :In th is paper o rthogonal experi m en t m ethod w as u sed to study the ex tracti on p rocess in alcoho l 2w ater system of fo lium A rte m isiae a rgy i .Its con ten ts of to tal flavono ids and to tal po lysaccharides w ere m easu red by co lo ri m etry .A nd it w as found that differen t facto rs i m pacting on ex tracti on w ere as fo llow ed :the concen trati on of alcoho l ,ex tracti on tem p eratu re ,ex tracti on ti m e and the rati o of m aterials fo r ex tracti on liqu id .T he an ti 2ox idan t activities of the ex tracti on liqu id w ere also studied by the chem ical lum inescence m ethod .R esu lts show ed that fo lium A rte m isiae a rgy i con tain s abundan t of flavono id and po lysaccharide ,

and its ex tract po ssesses strong an ti 2ox idan t activity .

T herefo re the researh and

developm en t of fo lium A rte m isiae a rgy i are of great sign ificance .

Key words :fo lium A rte m isiae a rgy i ;an ti 2ox idan t activity ;to tal flavono ids ;to tal po lysaccharides ;chem ical lum inescence

艾叶(Fo lium A rte m isiae a rgy i ),别名大艾叶、

杜艾叶、萎蒿,为菊科植物艾的叶。性温,味苦、辛;散

寒止痛,温经止血。艾叶中含有黄酮类、多糖类化合物,这些化合物具有较强的抗氧化活性[1]。

现有的研究表明,不少疾病的发生与发展与自由基有密切的关系,所以,直接或间接抑制自由基、清除自由基成了药物开发的一个突破口,而寻找一

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