生工生物常用载体测序引物列表

44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89
pDONR/Zeo pDONR221 pDrive pDsRED1-C1 pDSRED2-N1 pDsRed-Monomer-N1 pEASY-BLUNT pEASY-E1 pEASY-T1 pEASY-T3 pECFP-C1 pECFP-N1 pENTR/D-TOPO pET-*(His)
M13-20 M13-20 T7 pDsRED-C1-F pEGFP-N-5 pEGFP-N-5 M13-20 T7 M13-47/T7 M13-47/T7 pEGFP-C-5’ pEGFP-N-5’ M13-20 T7
M13-26 M13-26 SP6 pECFP-C-3 pDsRed1-N pDsRed1-N M13-26 T7 Ter M13-48 M13-48/SP6 pECFP-C-3' pEGFP-N-3’ M13-26 T7 Ter
常见载体引物表
序号 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 载体名称 pZeroBack/blunt P3xFlag-CMV-14 P3xFlag-CMV-7.1 P3xFlag-MYC-CMV-24 pACT pACT2 PACYCDUET-1(MCSI) PACYCDUET-1(MCSII) pAS2-1 PB42AD pBabe-puro PBACPAK8 pBAD PBIND pBK-CMV pBlueScript II SK(+) pBluescript SK (+) pBluescript SK (-) pBluescript KS (+) pBluescript KS (-) pBV220 PCANTB5E pCAT3-enhancer pcDNA3.0 pcDNA3.1 pcDNA4.0 pcDNA6/V5-His pcDNA6/myc-His pcDNAII pCEP4 pCI-neo pCMS-EGFP pCMV-3Tag-4A pCMV5-Flag pCMV-MYC/NUC pCMV-Sport pCMV-Tag pCMV-TNT PCOLDI pCR2.1-TOPO pCR3.1 pCS2 PDNR-LIB 正向引物(多个引物可根 据酶切位点选用) PJET1.2-F CMV-30 CMV-F CMV-F T7 5’AD DuetUP1 DuetUP2 5’BD PB42ADF pBABE5’ BAC1 PBAD-F 5’BD T7/M13-47 M13-47 M13-47 M13-47 M13-47 M13-47 PBV220+ S1 pGL3+ pEGFP-N5/T7 pEGFP-N5/T7 pEGFP-N5/T7 T7 T7 T7 pCMV5R/PCEP-F T7 T7 T3 pCMV5F pCMV5F M13-20/T7 T3 T7/SP6 pCold-F M13-47 T7 SP6 M13-20 反向引物(多个引物可根 据酶切位点选用) PJET1.2-R CMV-24 CMV-24 CMV-24 T3 3'AD DuetDOWN1 T7 Ter 3'BD PB42ADR pBABE3’ BAC2 PBAD-R T3 T3/M13-48 M13-48 M13-48 M13-48 M13-48 M13-48 PBV220S6 SP6/BGH BGH BGH BGH BGH SP6 pEGFP-N5/EBV-R T3 T3 T7 pCMV5R pCMV5R SP6/M13-26 T7 pCMV5R pCold-R M13-48 BGH T7 M13-26
PETA-IRES
PETDUET-1(MCSI) PETDUET-1(MCSII) pEYFP-C1 pEYFP-N1 pFastBac Dual (MCSI) pFastBac HT_A,B,C pFastBac1 pFLAG-CMV pGAD424 pGADGH pGADT7-Rec pGAPZa-A/B/C pGBKT7 pGEM-T(-Easy) pGEX-(*)T PGEX-6P-1 pGL2-Basic pGL2-Control Pgl3-BASIC pGL3-Enhancer PGL4.10 pIRES2-EGFP pJET1.2/blunt PLEXA
人源U6 鼠源U6 PLNCX-F 人源U6 PEGFP-N5 pLXSN-F MalE primer PIRES2-EGFP.P5/PMALC2X-F MalE primer M13-48 M13-47 M13-47 MSCV-F 5’AOX/a-factor 5'AOX M13-48 pQE30+ pQE30+ pQE30+ T7 DuetUP1 DuetUP2 T7 psi-check2-F M13-47 T7 人源U6 M13-20 T7 SP6 T7 SP6 T7 T7 T7 M13-47 M13-47 /T7 TRX-FORWARD T7 PQE30+/PTRC99C-F M13-47 M13-26/M13-48 M13-47/T7 T7 T7
PLNCX-R
pLXSN-R M13-47 PIRES2-EGFP.P5/PMALC2X-R M13-47 M13-47 M13-48 T7 Ter MSCV-R 3’AOX 3'AOX PBV220pQE30pQE30pQE30T7 Ter DuetDOWN1 T7 Ter BGH psi-check2-R 3.0rev 2.0rev M13-26 T3 SP6 T7 SP6 T3 M13-47 T3/M13-48 M13-48 TRX-REVERSE T7 Ter PBV220-/PTRC99C-R M13-48 M13-20/M13-47 M13-48 BGH SP6
90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135
PET-15B
pET22(a,b,c) pET28,30,24,49 pET32(a,b,c) pET-42a (-b, -c)(+) pET44 pET50
T7
T7 T7 T7/S.tag Stag T7/s.tag T7/s.tag
T7 Ter
T7 Ter T7 Ter T7 Ter T7 Ter T7 Ter T7 Ter DuetDOMN1 T7 Ter pEGFP-C3' pEGFP-N-3’ pECFP-C3 pECFP-C3 pECFP-C3 CMV-24/pFLAG-CMV-R 3'AD 3'AD 3'AD 3'AOX 3'BD M13-48 pGEX-3’ pGEX-3’ GLP2 GLP2 PGL3PGL3GLP2 pIRES2-EGFP.P3’ pJET1.2R PLEXA-R
EF-1aF
DuetUP1 DuetUP2 pEGFP-C5’ pEGFP-N5’ pFastBac-PH pFastBac-PH pFastBac-PH CMV30/pFLAG-CMV-F 5’AD 5’AD 5’AD/T7 a-FACTOR T7 M13-47 pGEX-5’ pGEX-5’ GLP1 GLP1 pGL3+ pGL3+ RVP3 pIRES2-EGFP.P5’ T7/pJET1.2F PLEXA-F
136
pYES2
T7
ຫໍສະໝຸດ BaidupYes2.R
PLK0.1 PLL3.7 PLNCX PLVX-SHRNA1 PLVX-IRES-NEO pLXSN pMAL-c2E pMAL-C2x pMAL-P2x PMD18-T PMD19-T pmirGLO pMIR-report pmscv pPIC9K pPICZa pPROEXHTa/b/c pQE2 pQE30 pQE40 pRSET PRSFDUET-1(MCSI) PRSFDUET-1(MCSII) pSectag(v5/His/a,b,c) psi-check2 pSilencer3.1-H1 hygro pSILENCEV2.0-U6 PsiREN-retroq pSK01-T pSK-CMV PSP64/65 pSP72 pSport1 pSTBlue-1 pSUPER pT7Blue(R) pTA2 pT-Adv PTHIOHISA,B,C pTO-T7 pTRC99a-c pUC18(19)/118(119) pUC57 pUCm-T pVAX1 pXT7