生物安全柜工作原理示意图)

生物安全柜工作原理示意图

生物安全柜：是为操作原代培养物、菌毒株以及诊断性标本等具有感染性的实验材料时，用来保护操作者本人、实验室环境以及实验材料，使其避免暴露于上述操作过程中可能产生的感染性气溶胶和溅出物而设计的。生物安全柜可提供样品和工作人员的双重保护。

Class II Type A1 BSC Characteristics

Exhaust approximately 30% of total air handled and recirculate 70% in cabinet

Minimum inflow air velocity is 75 fpm (0.38 m/s) through the front opening

May exhaust HEPA filtered air back into the room or through a canopy (to the outdoors)

Audiovisual alarm required when connected to a building exhaust system

May have contaminated ducts and plenums under positive pressure to the room

Type A1 cabinets are not suitable for work with volatile toxic chemical and volatile radionuclides.

生物安全柜工作原理示意图－图片说明：

Class II Type A1 BSC Characteristics Exhaust approximately 30% of total air handled and recirculate 70% in cabinet Minimum inflow air velocity is 75 fpm (0.38 m/s) through the front opening May exhaust HEPA filtered air back into the room or through

Biological Safety Cabinets

9.1 Introduction

When properly maintained and used in conjunction with good laboratory techniques, BSCs provide effective primary containment for work with human pathogens. In containment level 2 facilities, BSCs are used for procedures with the potential to produce infectious aerosols and for high concentrations or large volumes of infectious material. In containment levels 3 and 4, all open vessel activities with infectious materials are conducted in a BSC. Every employee working in a BSC must be trained in its correct use and have a good understanding of the different types of cabinets and how they work. Detailed information on the selection, function, and use of BSCs can be found elsewhere in the literature(1-3).

9.2 Classes and Characteristics of Biological Safety Cabinets

There are three classes of BSC: Class I, Class II and Class III. Selection of the proper class of BSC requires careful evaluation of the activities to be carried out. Horizontal, clean benches that direct air towards the operator are not biological safety cabinets and must not be used for handling infectious, toxic or sensitizing materials. Only cabinets that meet the National Sanitation Foundation (NSF) Standard No. 49-2002 (independent standard for the design, manufacture and testing of BSCs) and bear an NSF 49 seal should be purchased(4).

Class I Cabinets (Figures 1a and 1b)

These cabinets have unrecirculated airflow away from the operator that is discharged to the atmosphere after filtration through a HEPA filter. They provide good operator protection but do not protect the material