常用载体测序引物列表
载体通用引物序列
5'-GGC AAC TAG AAG GCA CAG TC-3'
PCDNA3.1F
5'-CTA GAG AAC CCA CTG CTT AC-3’
PCDNA3.1R
5'-TAG AAG GCA CAG TCG AGG-3’
pCEP Frorward
5'-AGC AGA GCT CGT TTA GTG AAC CG-3'
P31430
5'-CGT TTT AAAACCTAAGAGTCAC-3'
P12584
5'-TTT TCA GTA TCT ACG AT-3’
P17110
5'-TAC CAC TAC AAT GGA TG-3’
PAS2-1F
5'-TCA TCG GAA GCG AGT AG-3'
PAS2-1R
5'-CGT TTT AAA ACC TAA GAG TCA C-3'
PQE30R
5'-GTT CTG AGG TCA TTA CTG G-3'
pTARGET-SEQ
5'-TTA CGC CAA GTT ATT TAG GTG ACA-3’
PTRC99C-F
5'-TTG CGC CGA CAT CAT AAC-3’
PTRC99C-R
5'-CTG CGT TCT GAT TTA ATC TG-3’
PEGFP-C-3’
5'-TAT GGC TGA TTA TGA TCA GT-3'
PEGFP-C-5’
5'-CAT GGT CCT GCT GGA GTT CGT G-3'
常用pGEX载体图谱
常用pGEX载体图谱Rosetta系列的表达菌株可以提供T7 RNA聚合酶，它能表达PET系列载体上的外源基因。
pGEX系列载体上的外源基因不需要T7 RNA 聚合酶，普通的大肠杆菌经IPTG诱导即可表达Tac启动子是一组由Lac和trp启动子人工构建的杂合启动子，受Lac阻遏蛋白的负调节，它的启动能力比Lac和trp都强。
其中Tac 1是由Trp启动子的－35区加上一个合成的46 bp DNA片段(包括Pribnow 盒)和Lac操纵基因构成，Tac 12是由Trp的启动子-35区和Lac 启动子的-10区，加上Lac操纵子中的操纵基因部分和SD序列融合而成蛋白标签：A myc tag is a polypeptide proteintag derived from the c-myc gene product that can be added to a proteinusing recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. Itcan also be used in the isolation of protein complexes with multiple subunits.A myc tag can be used in many different assays that require recognition byan antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detectionby Western blotting.The peptide sequence of the myc-tag is (in 1- and 3-letter codes, respectively):N-EQKLISEEDL-C,N-Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu -C, where N stands for Amino-terminus and C stands for Carboxy terminus. The tag is approximately 1202 Daltons in atomic mass and has 10 amino acids.It can be fused to the C-terminus andthe N-terminus of a protein. It is advisablenot to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation intothe secretory pathway.A monoclonal antibody against themyc epitope, named 9E10, is available from the non-commercial Developmental Studies Hybridoma BankpGEX4T1载体基本信息出品公司: GE别名: pGEX-4T-1, pGEX4T1, pGEX 4T 1质粒类型: 大肠杆菌蛋白表达载体表达水平: 高拷贝启动子: Tac克隆方法: 多克隆位点，限制性内切酶载体大小: 4969 bp5' 测序引物及序列: pGEX5': GGGCTGGCAAGCCACGTTTGGTG3' 测序引物及序列: pGEX3': CCGGGAGCTGCATGTGTCAGAGG载体标签: N-GST载体抗性: Ampicillin 氨苄备注: 复制子是pMB1产品目录号: 27-4580-01稳定性: 瞬时表达 Transient组成型: 诱导表达病毒/非病毒: 非病毒pGEX4T1载体质粒图谱和多克隆位点信息原核生物DNA复制起始点，是DNA链上独特的具有起始DNA复制功能的碱基序列。
测序通用引物列表
测序通⽤引物列表测序通⽤引物列表载体名称上游引物（5'primer）下游引物（3'primer）P3*FLAG-CMV CMV-30(825-854) CMV-24(1129-1152) pAc5.1/V5-His(_A,_B,_C) pAc5-5 BGH rev pAcG2T pGEX-5 not availablepACT T7EEV T3/EBVrevpACT2 GAL4 ADfor pGAL4-ADrvpACT2 p17110 p12584pACYC184(BamHI-site) PBRforBam PBRrevBampACYCDuet-1(MCSI) ACYCDuetUP1 Primer DuetDOWN1pACYCDuet-1(MCSII) DuetUp2 T7-Terminator pAdEasy-1 not available not availablepAdTrack-CMV not available not available pAS2-1 GAL4 Bdfor/P24990 pGAL4-BDrv/P31430pB42AD pB42ADF PB42ADRpBABE pBMN_5'pBacPAK8 BAC1 BAC2pBAD pBAD-F pBAD-R pBAD/HisABC pBAD-5'pBBR1MCS T3/M13rev T7/M13forpBD-GAL4 pBD-GAL4-F PBD-GAL4-RpBI121 35S NOSpBIND GAL4-Bdfor T3,EBVrevpBK-CMV M13F/T7 M13R/T3 pBluescriptIIKS(+/-) T3/M13rev T7/M13for（优先⽤M13F）pBluescriptIISK(+/-) T3/M13rev T7/M13forpBluescriptKS(+/-) T3/M13rev T7/M13forpBluescriptSK(+/-) T3/M13rev T7/M13forpBR322(BamHI-site) PBRforBam PBRrevBampBR322(EcoRI/HindIII-sites) pBRforEco pBRrevHind pBR325(BamHI-site) PBRforBam PBRrevBampBR329(BamHI-site) PBRforBam PBRrevBam pBridge(MCSI) GAL4-Bdfor 3'BDpBridge(MCSII) not available not available pBS-T II T3/M13rev T7/M13for pBS（AMP）T7 T3 pBSR T3 T7ter pBudCE4.1(MCSI) CMV-Profor,T7 c-mycrev,EBVrevpBudCE4.1(MCSII) EF-1α Fwd BGHpBudCE4.1/lacZ/CAT CMV-Profor,T7 c-mycrev,EBVrev pBV220 pBV220F PBV220RpCAL-c T7/Tac-Profor T7-TerminatorpCAL-n T7/Tac-Profor T7-Terminator pCAMBIA1301(1300) pCAMBIA1301-5'(ECORI)/M13R pCAMBIA1301-3'(HindIII) pCAMBIA1304 pCAMBIA1301-5'(ECORI) pCAMBIA1301-3'(HindIII) pCAMBIA2300 M13R(-48) M13F(-47)/M13F(-49) pCANTB5E S1 S6pCAT3-enhancer RVP3 此载体⽆反向引物pCDFDuet-1(MCSI) ACYCDuetUP1 Primer DuetDOWN1pCDFDuet-1(MCSII) DuetUP2 T7TER pcDNA1.1 CMV-Profor,T7 pCDM8-3pcDNA2.1 M13for/T7 M13rev/Tac-ProforpcDNA3 T7/CMV-Profor BGH rev/SP6 pcDNA3.1(+/-) pcDNA3.1F/T7/CMV-Profor BGH rev pcDNA3.1/His(_A,_B,_C)T7/CMV-Profor BGH rev pcDNA3.1/myc-His(_A,_B,_C) T7/CMV-Profor BGH rev/c-mycrev pcDNA3.1/V5-His(_A,_B,_C) T7/CMV-Profor BGH rev/V5rev pcDNA3.1/Hygro(+,-) T7/CMV-Profor BGH rev/V5revpcDNA3.1/Zeo(+.-) T7 BGHrevpcDNA3.3TOPOTA CMV-Profor TKPAreverse pcDNA4/HisMax(_A,_B,_C) Xpressfor BGH revpcDNA4/HisMax-TOPO Xpressfor BGH rev pcDNA4/HisMax-TOPO/lacZ Xpressfor BGH rev pcDNA4/myc-His(_A,_B,_C) T7,CMV-Profor BGH rev,c-mycrev pcDNA4/TO TREfor/CMV-Profor BGH rev pcDNA4/V5-His(_A,_B,_C) CMV-Profor,T7 BGH rev,V5rev pcDNA4-TET/ON/AMP+ CMV-ProforpcDNA5/FRT T7/CMV-Profor BGH rev pcDNA6/myc-His(_A,_B,_C) CMV-Profor,T7 BGH rev,c-mycrev pcDNA6/V5-His(_A,_B,_C) CMV-Profor,T7 BGH rev,V5rev pCEP4 pCEP-F/CMV-Profor EBVrevpCI T7（17BASE）EBVrevpCI-neo T7（17BASE）EBVrev,T3pCMS-EGFP T7 T3pCMS-EGFP T7 EBVrev,T3pCMV-3Tag-4A T3 /PFLAG-CMV-F T7 pCMV5 pCMV5F PCMV5RpCMV6 CMV-Profor pCMV6-3、HGHrevpCMV6-XL4 CMV-Profor/T7 pCMV6-3、HGHrevpCMV-HA PCMV-F,CMV-Profor,TREfor PCMV-R，EBVrev pCMV-MYC(AMP+) PCMV-F PCMV-R, EBVrev pCMVmyc/nuc CMV-Profor BGH rev,c-mycrev pcmv-scriptEXVector T3 T7 pCMV-Sport6 T7/M13for SP6/M13rev pCOLADuet-1(MCSI) ACYCDuetUP1 Primer DuetDOWN1pCOLADuet-1(MCSII) DuetUP2 T7TER pCR2.1 T7,M13for M13rev pCR2.1-TOPO M13for,T7 M13rev pCR3.1 T7 BGH rev pCR3.1-Uni T7 BGH rev pCR4 T3/M13rev T7/M13for pCR4-Blunt T3/M13rev T7/M13forpCR4-TOPO M13rev,T3 M13for,T7pCR-Blunt M13rev T7,M13forpCR-BluntII M13rev/SP6 T7/M13for pCR-BluntII-TOPO M13rev/SP6 M13for/T7 pCRII M13rev/SP6 T7/M13for pCRII-TOPO M13rev/SP6 M13for/T7pCR-ScriptSK(+) T7/M13for T3/M13revpCR-XL-TOPO M13rev T7,M13forpCS2+(MCSI) SP6 EBVrev,T7?pCS2+(MCSII) T3 pSG5-3pDB_GAL4cam T7PpDB_leu PDBLeu-5 PDBLeu-3pDEST22 DEST22F DEST22RpDEST15 pGEX-5 T7-TerminatorpDEST20 pGEX-5 pFastBac-3pDEST26 TREfor/CMV-Profor T7/M13forpDisplay T7/CMV-Profor c-mycrev pDNR-LIB(MCS_A) M13for,T7 pSG5-3,M13R pDonar M13F M13RpDONR201 PDONR-F PDONR-RpDONR207 PDONR-F PDONR-RpDONR223 M13F M13RpDrive T7/M13rev SP6/M13for pDsRED1-C1 (DsRed1-N-f) DsRed1-C-rpDsRed1-N1 CMV-Profor RFP-Nrev,CMV-R?pDsRed2-N1 (DsRed1-N-f) DsRed1-C-rpDsRed2-C1 (DsRed1-N-f) DsRed1-C-r pDsRED2-C1(KAN+) pDsRED-ex-C1-F pEGFP-N-3’ pDsRed-ExpressdsRed1_N_primer/CMV-F dsRed1_C_primer/CMV-R pDSRED-monomer-N1(KAN+) dsRed1_N_primerpDSRED-N1(KAN+) pEGFP-N-5’ pDSRED-N-RpEASY-Blunt M13F T7/ M13RpEASY-BluntSimple M13F T7/ M13R（距离插⼊位点合适，优先安排）pEASY-T1 Simple M13F M13R（距离插⼊位点合适，优先安排）pEASY-T1 M13F/T7 M13RpEASY-T3 M13F/T7 M13R/SP6pECFP-N(C) PECFP-N-5. PECFP-N-3,pEF/myc/cyto/ER/mito/nuc pEF-5 Pcdna3.1R/BGH rev pEF1(4,6/myc-His) T7/pEF-5 Pcdna3.1R/BGH rev pEF1(4,6/V5-His) T7/pEF-5 Pcdna3.1R/BGH rev pEF1(4;6/His_A;_B;_C) T7/Xpressfor Pcdna3.1R/BGH rev pEF5/FRT/V5-D(-TOPO) T7/pEF-5 Pcdna3.1R/BGH rev pEGFP M13rev EGFP-NrevpEGFP-1 not available EGFP-Nrev/ PEGFP-N-3′pEGFP-C PEGFP-C-5′ PEGFP-C-3′pEGFP-C1 EGFP-Cfor SV40-pArevpEGFP-C2 EGFP-Cfor SV40-pArevpEGFP-C3 EGFP-Cfor SV40-pArevpEGFP-F EGFP-Cfor SV40-pArevpEGFP-N PEGFP-N-5′ PEGFP-N-3′pEGFP-N1 CMV-Profor EGFP-NrevpEGFP-N2 CMV-Profor EGFP-NrevpEGFP-N3 CMV-Profor EGFP-NrevpENTR M13F M13R pET-11(-a,-b,-c,-d) T7 T7-TerminatorpET-12(-a,-b,-c) T7 T7-Terminator pET-14b T7 T7-TerminatorpET-15b T7 T7-TerminatorpET-16b T7 T7-TerminatorpET-17b T7 T7-TerminatorpET-17xb T7 T7-TerminatorpET-19b T7 T7-TerminatorpET-20b(+) T7 T7-TerminatorpET-21(-a,-b,-c,-d)(+) T7 T7-Terminator pET-22b(+) T7 T7-TerminatorpET-23(-a,-b,-c,-d)(+) T7 T7-TerminatorpET-24(-a,-b,-c,-d)(+) T7 T7-Terminator pET-25b(+) T7 T7-TerminatorpET-26b(+) T7 T7-TerminatorpET-27b(+) T7 T7-Terminator pET-28a(-b,-c)(+) T7 T7-TerminatorpET-29a(-b,-c)(+) S.tag/T7 T7-TerminatorpET-3(-a,-b,-c,-d) T7 T7-Terminator pET-30_Ek/LIC T7 T7-Terminator pET-30_Xa/LIC T7 T7-Terminator pET-30a(-b,-c)(+) S.tag/T7 T7-Terminator pET-31b(+) not available T7-Terminator pET-32_Ek/LIC TRXfor T7-Terminator pET-32_XA/LIC TRXfor T7-Terminator pET-32a(-b,-c)(+) S.tag/TRXfor T7-Terminator pET-33b(+) T7 T7-Terminator pET-34b(+) S.tag T7-Terminator pET-35b(+) S.tag T7-Terminator pET-36b(+) S.tag T7-Terminator pET-37b(+) S.tag T7-Terminator pET-38b(+) not available T7-Terminator pET-39b(+) S.tag T7-Terminator pET-40b(+) S.tag T7-Terminator pET-41_Ek/LIC GST-Cfor T7-Terminator pET-41a(-b,-c)(+) S.tag/pGEX-5 T7-Terminator pET-42a(-b,-c)(+) S.tag/pGEX-5 T7-Terminator pET-43.1a(-b,-c) (+) S.tag coliDOWN pET-43.1a_EK/LIC S.tag not available pET-44a(-b,-c)(+) S.tag T7-Terminator pET-44a_EK/LIC S.tag not available pET-45b(+) T7 T7-Terminator pET-46_Ek/LIC T7 T7-Terminator pET-5(-a,-b,-c) T7 T7-Terminator pET-52b(+) T7 T7-Terminator pET-7 T7 T7-Terminator pET-9(-a,-b,-c,-d) T7 T7-Terminator pETcoco-1 T7 T7-Terminator pETDuet-1(MCSI) pETUP1 DuetDOWN1 pETDuet-1(MCSII) DuetUp2 T7-Terminator pET-T4 T7 T7-Terminator pEYFP-C1 pEGFP-C-5’pEGFP-C-3' pEYFP-N1 pEGFP-N-5’,CMV-Profor pEGFP-N-3’ pFLAG-ATS N26for pTrcHis-rv；C24rev pFLAG-CTC N26for pTrcHis-rv；C24rev pFLAG1 N26for pTrcHis-rv；C24rev pFLAG-CMV(-2) CMV-30；CMV-Profor CMV-24 pFLAG-CMV1 CMV-30 CMV-24pGAD424 GAL4_ADfor 3'ADpGADGH GAL4_ADfor 3'ADpGADT7 T7/GAL4_ADfor 3'AD pGADT7-Rec T7/GAL4_ADfor 3'AD pGADT-T T7 3,BD pGAD10 GAL4_ADfor 3'AD pGAPZa-A a-FACTOR 3'AOX pGBKT7 T7 3,BDpGBT9 MATCHMAKER5'DNA-BD/GAL4BD MATCHMAKER3'DNA-BDpGEM-1 T7 SP6pGEM-3 T7 SP6 pGEM-3Zf(+)(-) SP6/M13rev T7/M13for pGEM-4 T7 SP6pGEM-4Z T7/M13for SP6/M13rev pGEM-5Zf(+)(-) SP6/M13rev T7/M13for pGEM-7Zf(+)(-) SP6/M13rev T7/M13for pGEM-9Zf(+)(-) T7/M13for SP6/M13rev pGEM-T T7/M13for SP6/M13rev pGEM-T_Easy T7/M13for SP6/M13rev pGene/V5-His_A(_B,_C) pGENE-5 BGH rev,V5rev pGEX-2T pGEX-5 pGEX-3pGEX-2TK pGEX-5 pGEX-3pGEX-3X pGEX-5 pGEX-3 pGEX-4T-1(-2,-3) pGEX-5 pGEX-3 pGEX-5X-1(-2,-3) pGEX-5 pGEX-3 pGEX-6P-1(-2,-3) pGEX-5 pGEX-3 pGL2 GLP1 GLP2pGL3 RVP3 GLP2(RVP4)(没有特别说明⽤GLP2)pGL3-Basic(MCSI) RVP3(PGL3+)? GLP2(PGL3-)?pGL3-Basic(MCSII) RVP4, PLXSN-3'?pGL4pGlow(-TOPO) T7 pGlow-3 pGM-T T7 SP6pGWC SP6 T7pHook-2 T7/CMV-Profor not availablepIND/V5-His_A(_B,_C) not available BGH rev,V5rev PinpointTMVector PinpointPrimer SP6pIRES(MCSI) T7 pIRES-3pIRES(MCSII) not available T3,EBVrevpIRES2-EGFP CMV-Profor,pIRES2-EGFP.P5’ IRESrev,pIRES2-EGFP.P3’ pIRES-hrGFP-2a CMV-Profor,T3 pIRES-hrGFP-3 pIRESneo2 T7/CMV-Profor pIRES-3pIVEX(all) T7/pBRrevBam T7-TerminatorpLEXA pLEXA-F pLEXA-RpLNCX pLNCX-F pLNCX-RpLNCX2 CMV-Profor pRevTRE-3 pLVTHM（H1启动⼦下游）H1primer SP6 pLXSN pLXSN-F pLXSN-RpMALc MalE primer M13F(-47)pMAL-c2E MalE primer M13F(-47)pMAL-C2x Pmal-c2x-F(P5’) Pmal-C2X-R(P3’ )pMALd MalE primer M13F(-47)pMALe MalE primer M13F(-47)pMAL-P4X MalE primer M13F(-47)pMAL-p2X MalE primer M13F(-47)pMET A/B/C AUG1F AUG1RpMD18-T M13R(-48)ECORI M13F(-47)(HINDⅢ)pMD19-T M13F47 M13R48pMD20-T M13F47 M13R48pMOSBlue T7/M13rev M13forpMSCV-puro pLXSNfw pMSCVrev pMT/V5-His_A(_B,_C) Mtfor BGH rev,V5rev pNTAP T3 T7pCTAP pCTAP5'primer T7pOTB7 M13rev/T7 M13for/SP6 pOptiVEC-TOPO TA CMV-F/CMV-Profor EMCVIRES reverse pPC86 PPC86-F PPC86-R pPC97 PBD-GAL4-F PBD-GAL4-RpPIC3.5K 5'AOX 3'AOXpPic9k(A+,Z+) 5’AOX(Ppic9k-f)/a-factor 3’AOX(Ppic9k-R) pPICZa 5'AOX/PPICZa-F 3'AOX pPROEXHTA M13R(-48) ⽆反向引物pProEX-HTa(b,c) M13R48/ Tac-Profor pBADrv/pTrcHis-3 pPD129.36 M13F20/M13F pQE30or40or60(AMP+) PQE30-F PQE-30R pRC/CMV2/CAT CMV/T7pRECEIVER CMV-F 此载体⽆反向引物pRECEIVER-M03 pRECEIVER-M03F pRECEIVER-M03R pREP4 pREP4 forward primer EBV reverse primerpRK5 SP6 pSG5-3pRSET T7/Xpressfor T7ter pRSFDuet-1(MCSI) DuetUP1 DuetDOWN1 pRSFDuet-1(MCSII) DuetUP2 T7TER pSecTac2T7/CMV-Profor BGH rev/c-mycrevpSG5 pSG5-5,/T7 PSG5-R(SV40)pSHlox1 T7 SP6 pSHUTTLE-CMV PSHUTTLE-CMV-F PSHUTTLE-CMV-R pSI T7 EBVrev pSilencer1.0-U6 T7 T3pSilencer2.0-U6 T7 M13for pSilencer2.1-U6neo T7 M13for pSilencer3.1-H1hygro M13F(-47) 3.0REV pSilencer4_1-CMVneo pSilener4.1-5' CMV-Profor pSILENCEV2.0-U6 T7 2.0REV pSIneo T7 EBVrev,T3Psit T7 T7T pSK01-T M13F M13RpSK-CMV(KAN+) T7 T3 pSOS Psos5' Psos3'pSP64 SP6 M13revpSP64/65 SP6 此载体⽆反向引物pSP65 SP6 M13revpSP70 SP6 T7pSP71 SP6 T7pSP72 SP6 T7pSP73 SP6 T7pSport1 SP6/M13F(-47) T7/M13RpSPORT1 SP6/M13for T7/M13revpSPT18 SP6 T7pSTBlue T7/M13rev SP6/M13for pSUPER.Basic T7/M13F M13R pSUPER T7 T3pSURE-T M13F M13R pSURE-Tsimple M13F-47 M13R-48 pSuperCos pBRforEco T7pT3T7 M13rev/T3 M13for/T7pT7-7 T7 not availablepT7Blue? T7 M13F(-47)pT7T3_18U M13rev/T7 M13for/T3 pT7T3D-PAC M13rev/T7 M13for/T3 pTA2 M13F/T7(优先使⽤M13F）T3/M13RpT-Adv M13rev T7,M13forpTAg T7/M13rev M13for pTARGETTM pTarget.F(在T7前⾯）/T7 pTarget.R pTG19-T M13F(-47) M13R(-48) pTARGETTM T7/M13F PTARGET-SEQ pThioHisA,B,andC Trx Forward Trx Reverse / M13forpTO-T7 T7 T7TERpTRC99A PTRC99A-F pTrcHis-rvpTrc99a-c PTRC99C-F PTRC99C-RpTRC99a-c PTRC99C-F PTRC99C-RpTrcHisB PTrcHisA-F/Xpressfor PTrcHisA-RpTrcHisA PTrcHisA-F PTrcHisA-RpT-REX-DEST30 TREfor T7 pTriEx1 T7/TriExup TriExdownpTriEx1.1 T7/TriExup TriExdownpTriEx1.1_Hygro T7/TriExup not availablepTriEx1.1_Neo T7/TriExup IRESrevpTriplEX1 TriplEx-LD-5 T7,TriplEx-LD-3pTriplEX2 TriplEx-LD-5；pTR5‘ T7,TriplEx-LD-3pTWIN-1 T7 T7TERpTXB1 T7 MxeInteinrevpTXB2 T7 MxeInteinrevpTXB3 T7 MxeInteinrevpTYB1 T7 InteinrevpTYB2 T7 InteinrevpTYB3 T7 InteinrevpTYB4 T7 InteinrevpTZ_18U T7/M13rev M13forpUB6/V5-His UB-F BGH rev,V5rev pUC13 M13rev/M13rev(-48) M13for/M13for(-49)pUC18 M13rev/M13rev(-48) M13for/M13for(-49) pUC18(19)/118(119) M13F(-47) M13R(-48)/PUC19(HINDⅢ) pUC19M13rev/M13rev(-48) M13for/M13for(-49)pUC57 M13rev/M13rev(-48) M13for/M13for(-49)pUC8 M13rev/M13rev(-48) M13for/M13for(-49)pUC9 M13rev/M13rev(-48) M13for/M13for(-49)pUM-T M13F(-20) M13R(-20)pUCm-T(AMP+) M13F/T7 M13RpUNI/V5-His not available BGH rev,V5revpVAX1T7 T7/CMV-Profor PCDNA3.1R/BGHR pVP22/mycHis VP22-Cfor BGH rev,c-mycrev pVP22/mycHis2 T7/CMV-Profor VP22-Nrev pWE15 pBRrevHind T7pYES2 T7 pYes2.R pYES-DEST52 T7/GAL1-Profor V5rev/CYC1-Terminator pYEX_4T_1 Clontech pYEX_4T-3pYEX_4T_2 pGEX-5 pYEX_4T-4pYEX_4T_3 pGEX-5 pYEX_4T-4pYEX_4T_4 pGEX-5 pYEX_4T-4 pZeoSV2+/hu-Tyr T7 not available pZERO-1 M13rev/SP6 T7/M13forpZERO-2 M13rev/SP6 M13for/T7pZome-1-C Zome-F Zome-RpZome-1-N Zome-F Zome-R YCp50(BamHI-site) PBRforBam PBRrevBam YEp13(BamHI-site) PBRforBam PBRrevBam YEp24(BamHI-site) PBRforBam PBRrevBam YIp5(BamHI-site) PBRforBam PBRrevBam RNAi-ReadypSIREN-RetroQ-U6ZsGreen U6菌PCDB T7 BGH 天为时代TA载体T7 T3 m13（+）T7？T3？pMSCV-neo pMSCV-5' pMSCV-3'。
常用载体测序引物列表
常用载体测序引物列表常用载体测序引物列表载体名称正向引物反向引物pACT T7 T3pACT2 GAL4 AD(5’AD) 3'AD/pACT2-R PACYCDUET-1(MCSII) DuetUP2 T7TERpAD/pl-DEST CMV-FpAD-Track pAD-Track-F 无pAS2-1 5’BD M13-26/48 PB42AD PB42ADF PB42ADR PBAD PBAD-F PBAD-R pBABE pBABE5’ pBABE3’ PBACPAK8 BAC1 BAC2 pBK-CMV T7/M13-20 T3/M13-26 PBS(SK/KS)/M13- M13F/T7T3/M13R pBV220 PBV220F PBV220R pBI121需根据酶切位点确认需根据酶切位点确认 pCAMBIA 1301(1300) 需根据酶切位点确认需根据酶切位点确认pCAMBIA 2300 需根据酶切位点确认需根据酶切位点确认PCANTB5E S1/M13R S6pCAT3-enhancer pGL3+pcDNA3.0 pEGFP-N5/T7 SP6/BGHpcDNA3.1 pEGFP-N5/T7 BGHpcDNA4 T7/pEGFP-N5 BGHpcDNA6 T7/pEGFP-N5 BGHpcDNAII T7SP6 pCE2.1 M13FM13R pCEP4 pCMV5R/PCEP-F pEGFP-N5/EBV-R pCF-T M13FM13R pCIT7（17Base ） pCMV5R pCI-neo T7（17Base ） T3pCMS-EGFP T7T3 pCMV-3Tag-4A T3 /pFlag-CMV-F T7pCMV5 pCMV5F pCMV5R pCMV5-Flag pCMV5FpCMV5R pCMV-MYC/NUC pCMV5FpCMV5R PCMV-HA pCMV5FpCMV5R pCMV-SportSP6/M13RpCMV-Tag T3T7 pCMV-TNT T7/SP6pCMV5R pCR2.1-TOPO M13F/T7M13R pCR3.1 T7BGH pCS2 SP6T7PDNR-LIB M13F M13RpDonar M13F M13R pDONR201 pDONR-F pDONR-R pDONR221 M13F M13RpDrive T7 SP6 pDRIveR T7 SP6pDsRED1-C1 pDsRED-C1-F pECFP-C-3 pDsRed1-N1 pEGFP-N-5 RFP-NrevpDsRed-Monomer-N1 pEGFP-N-5 pDsRed1-NpDsRED2-C1 pDsRED-ex-C1-F pEGFP-N-3’pEGFP-N-5 pDsRed1-N pDSRED2-N1pDSRED-N1 pEGFP-N-5’ PDSRED-N-R pEASY-BLUNT M13F/T7 M13RTerpEASY-E1 T7 T7pEASY-T1 M13F/T7 M13RpEASY-T3 M13F/T7 M13R/SP6 pECFP-C pEGFP-C-5’ pECFP-C-3' pECFP-N1 pEGFP-N-5’ pEGFP-N-3’pEF/myc/ER pEF-F pCDNA3.1R pEGFP-C pEGFP-C-5’ pEGFP-C-3' pEGFP-N pEGFP-N-5’ pEGFP-N-3’ pENTR/D-TOPO M13F M13RTerpET-*(His) T7 T7pET22(a,b,c) T7 T7TerTerpET28,30,24,49 T7 T7TerpET32(a,b,c) T7/S.tag T7pET-42a (-b, -c)(+) Stag T7 TerpET50 T7/s.tag T7TERpET44 T7/s.tag T7Ter pEYFP-N1 pEGFP-N-5’ pEGFP-N-3’ PETBlue M13-20 PETBlueDOWN PETDUET-1(MCSI) PBRrevBam DuetDOMN1 PETDUET-1(MCSII) DuetUP2 T7TERpEYFP-C1 pEGFP-C-5’ pEGFP-C-3' pEYFP-N1 pEGFP-N-5’ pEGFP-N-3’ pFastBac pFastBac-F pFastBac-R pFastBac HT_A,B,C pFastBac-PH pECFP-C-3 pFastBac1 pFastBac-PH pECFP-C-3 pFastBac Dual (MCSI) pFastBac-PH pECFP-C-3 pFLAG-CMV CMV30/pFLAG-CMV-F CMV-24/pFLAG-CMV-Rp3xFlag-CMV-7.1 CMV-F/CMV24 CMV30pGAD424 5’AD 3'ADpGADGH 5’AD 3'ADpGADT7-Rec 5’AD/T7 3'ADpGAPZa-A/B/C a-FACTOR 3'AOXforward 3'AOXpGAPZ-A/B/C pGAPpGBKT7 T7 3'BDpGEM-T(-Easy) M13F/T7 SP6/M13RpGEX-(*)T pGEX-5’ pGEX-3’pGL2 pCMV5R PGL3-Pgl3-BASIC pGL3+ PGL3-pGL3-Enhancer pGL3+ PGL3-PinPoint TM PinPoint Primer SP6pIRES PIRES-F/T7 T3/PIRES-RpIRES2-EGFP pIRES2-EGFP.P5’pIRES2-EGFP.P3’ pLenti6/v5-Dest pEGFP-N-5’PLEXA PLEXA-F PLEXA-R PLNCX PLNCX-F PLNCX-R pLXSN pLXSN-F pLXSN-RPjc1-tac T7pJet.1.21.Blunt T7primer M13F(-47) pMAL-c2E MalEP3/PMAL-C2X-R pMAL-C2x P5/PMAL-C2X-Fprimer M13F(-47) pMAL-p2X MalEPMD18-T M13R(-48) M13F(-47) PMD19-T M13F(-47) M13R(-48) pMIR-report M13FPPC86 PPC86-F PPC86-R3’AOXpPIC9K 5’AOX/a-factor3'AOX pPICZa 5'AOX/PPICZa-FpPROEXHTA M13R(-48)pQE30or40 pQE30+ pQE30- pRECEIVER CMV-FpRSET T7 T7TER PRSFDUET-1(MCSI) DuetUP1 DuetDOWN1 PRSFDUET-1(MCSII) DuetUP2 T7TERpShuttle-CMV pShuttle-CMV-F pShuttle-CMV-R PSILENCE-1.0-U6 T7 T3pSilencer3.1-H1 hygro M13F(-47) 3.0revpSILENCEV2.0-U6 T7 2.0revpSK01-T M13F M13RpSK-CMV T7 T3Psos H1PSP64/65 SP6pSP72 T7 SP6T7/M13RpSport1 SP6/M13F(-47)pSTBlue-1 T7 SP6pSUPER T7 T3pT7Blue(R) T7 M13F(-47)pTA2 M13F/T7 T3/M13R/T7 M13RpT-Adv M13FpTARGET TM pTarget-F/T7 arget-R PTHIOHISA,B,C TRX-FORWARD TRX-REVERSEpTO-T7 T7 T7TERPBV220-/PTRC99C-R pTRC99a-c PQE30+/PTRC99C-F pTriPLEx2 PT5/5'TriplEx2 T7pTz57r/t M13+ M13-pTWIN-1 T7 T7TERpUC18(19)/118(119) M13F(-47) M13R(-48)M13F(-47)/M13F pUC57 M13R(-48)/M13RpUCm-T M13F/T7 M13RpVAX1 T7 BGHT3/M13R/M13R(-48) pWSK29 T7/M13F/M13F(-47) pXT7 T7 SP6pYES2 T7 pYes2.R。
载体与引物表(完全)
SP6 PYES2.R
M13R T3
载体抗性 Amp+
Amp+ Amp+ Amp+ Amp+ Amp+ Amp+ Amp+ Amp+
Amp+ Amp+ Amp+ Amp+
共3第3页
PM
PM-F
pMAL-c2E
MalE primer
pMAL-C2x
P5’
pMAL-p2X
MalE primer
pMD18-T
PQE30F/M13R(-48)/RV-M
Pmep4
pMEP4-F
pPIC9K(A+,Z+)
5’AOX(pPIC9K-F)
PPICZa pPROEXHTA
5'AOX/PPICZa-F M13R(-48)
pcDNA3.1R BGH
Amp+
M13F
M13R
pCEP-F pCI-F2/ T7(17base)
EBV-R (此载体没有反向引物)EBR
pCI-F2/ T7(19/17base)
T3
Amp+
T7 2G
T3
pCMV-F
pCMV-R
T3 /PFLAG-CMV-F
T7
Pcmv5f
Pcmv5r
Amp+
Pcmv5f
PDC316.R Primer(R)
载体抗性
pDonar
M13F
M13R
pDONR207
FORWARD
REVERSE
Pdonr221
M13F
常见的通用引物序列
5’TGCTAGTTATTGCTCAGCGG3'
S.TAG
5’CGAACGCCAGCACATGGACA3’
T3
5'ATTAACCCTCACTAAAGGGA3’
M13F（47）
5'CGCCAGGGTTTTCCCAGTCACGAC3’
M13R（48)
5'AGCGGATAACAATTTCACACAGGA3'
TGC GTA CTG CGG TGA TCA AC
P3'
CTG CAA GGC GAT TAA GTT GG
DuetDOWN1
5’-GATTATGCGGCCGTGTACAA-3'
PET Upstream
ATG CGT CCG GCG TAG AGG AT
DuetUP2
5'-TTGTACACGGCCGCATAATC-3’
PTRCHISAF
5’CAT GGT ATG GCT AGC ATG AC3’
PTRCHISAR
5'CAG GCT GAA AAT CTT CTC TC3'
pDC316 F
5’ —TAA CTT GTT TAT TGC AGC —3'
pDC316 A
5' -TAT GCT TAC CGT AAC TTG -3'
PEFF
5' TCA AGC CTC AGA CAG TGG TTC3’
PCEPF
5’ AGA GCT CGT TTA GTG AAC CG3’
EBVR
5’ GTG GTT TGT CCA AAC TCA TC3’
CMVF
5'CGCAAATGGGCGGTAGGCGTG3'
8 常用载体通用引物
EF-1α Fwd
BGH
pBudCE4.1/lacZ/CAT
CMV-Profor,T7
c-mycrev,EBVrev
pBV220
pBV220F
PBV220R
pCAL-c
T7/Tac-Profor
T7-Terminator
pCAL-n
T7/Tac-Profor
T7-Terminator
M13rev/Tac-Profor
pcDNA3
T7/CMV-Profor
BGH rev/SP6
pcDNA3.1(+/-)
pcDNA3.1F/T7/CMV-Profor
BGH rev
pcDNA3.1/His(_A,_B,_C)
T7/CMV-Profor
BGH rev
pcDNA3.1/myc-His(_A,_B,_C)
BAC1
BAC2
pBAD
pBAD-F
pBAD-R
pBAD/HisABC
pBAD-5'
pBBR1MCS
T3/M13rev
T7/M13for
pBD-GAL4
pBD-GAL4-F
PBD-GAL4-R
pBI121
35S
NOS
pBIND
GAL4-Bdfor
T3,EBVrev
pBK-CMV
M13F/T7
M13R/T3
载体名称
上游引物（5'primer）
下游引物（3'primer）
P3*FLAG-CMV
CMV-30(825-854)
CMV-24(1129-1152)
pAc5.1/V5-His(_A,_B,_C)
表4 主要使用的引物及序列
表4 主要使用的引物及序列序列技术在现代生物学研究中发挥着越来越重要的作用。
在进行序列分析之前，需要先选择合适的引物，以确保检测到所需的目标序列，并避免检测到不必要的杂交或引物间的相互杂交。
本文将介绍主要使用的引物及其序列。
1. 基因测序引物基因测序引物是用于测序DNA或RNA的引物。
这些引物通常包括一个DNA或RNA序列和一个与之匹配的引物序列。
常用的基因测序引物包括以下几种：- M13引物M13引物是一种通用的测序引物，用于测序单链DNA。
其序列为：5'-GTAAAACGACGGCCAGT-3'。
- T7和SP6引物T7和SP6引物用于测序DNA文库中的克隆DNA。
其中T7引物的序列为：5'-TAATACGACTCACTATAGGG-3'，SP6引物的序列为：5'-ATTTAGGTGACACTATAG-3'。
- M13F和M13R引物M13F和M13R引物也用于测序单链DNA。
M13F引物的序列为：5'-GTAAAACGACGGCCAGT-3'，M13R引物的序列为：5'-CAGGAAACAGCTATGAC-3'。
- T3和T7引物T3和T7引物与RNA兼容，并用于测序RNA和RNA-DNA杂交分子。
T3引物的序列为：5'-AATTAACCCTCACTAAAGGGA-3'，T7引物的序列为：5'-TAATACGACTCACTATAG-3'。
2. PCR引物PCR引物是用于荧光定量PCR、数字PCR、标准PCR等技术的引物。
以下是几种常用的PCR引物：- GAPDH引物GAPDH引物用于检测人体组织中的GAPDH基因表达水平。
其序列为：5'-GAAGGTGAAGGTCGGAGT-3'（前引物）、5'-GAAGATGGTGATGGGATTTC-3'（后引物）。
载体及通用引物
S1
S6
pCAT3-enhancer
RVP3
此载体无反向引物
pCDFDuet-1(MCSI)
ACYCDuetUP1 Primer
DuetDOWN1
pCDFDuet-1(MCSII)
DuetUP2
T7TER
pcDNA1.1
CMV-Profor,T7
pCDM8-3
pcDNA2.1
M13for/T7
EGFP-Nrev/ PEGFP-N-3′
pEGFP-C
PEGFP-C-5′
PEGFP-C-3′
pEGFP-C1
EGFP-Cfor
SV40-pArev
pEGFP-C2
EGFP-Cfor
SV40-pArev
pEGFP-C3
EGFP-Cfor
SV40-pArev
pEGFP-F
EGFP-Cfor
SV40-pArev
T7
T7-Terminator
pET-25b(+)
T7
T7-Terminator
pET-26b(+)
T7
T7-Terminator
pET-27b(+)
T7
T7-Terminator
pET-28a(-b,-c)(+)
T7
T7-Terminator
pET-29a(-b,-c)(+)
S.tag/T7
T7-Terminator
T7-Terminator
pET-20b(+)
T7
T7-Terminator
pET-21(-a,-b,-c,-d)(+)
T7
测序DNA引物列表
M13F M13F（-47）
5′-TGT AAA ACG ACG GCC AGT-3’ 5′-CGC CAG GGT TTT CCC AGT CAC GAC-3’
M13F-21
5′-TGT AAA ACG ACG GCC AGT-3’
M13R M13R（-48）
5′-CAG GAA ACA GCT ATG ACC-3’ 5′-AGC GGA TAA CAA TTT CAC ACA GGA-3’
P12584
5′-TTT TCA GTA TCT ACG AT-3’
P17110
5′-TAC CAC TAC AAT GGA TG-3’
pbd-gal4- f
5′-GCC TCT AAC ATT GAG ACA GC-3’
pbd-gal4-r
5′-AAG AGT TAC TCA AGA ACA AGA A-3’
PIRES2-EGFP-F 5′-GTA GGC GTG TAC GGT GGG AG-3’
PIRES3-EGFP-R 5′-AAC GCA CAC CGG CCT TAT TC-3’
pMAL-C2X-F pMAL-C2X-R PQE30F PQE30R RVP3 RVP4 S.TAG SP6 T3 T7 T7（17BASE） T7TER NOSR
5′ -TGT ATC TTA TGG TAC TGT AAC TG-3’
GLP2
5′ -CTT TAT GTT TTT GGC GTC TTC CA-3’
M13(-96)
5′-CCC TCA TAG TTA GCG TAA CG-3’
M13-20
5′-GTA AAA CGA CGG CCA GTG-3’
A-FACTOR
常见载体的测序引物
常见载体的测序引物常见载体的测序引物：Primer of Vector:Vector：Primer(F)；Primer(R)pACT T7 T3pACT2 GAL4 AD pACT2-RpAS2-1 GAL4 BD pAS2-1.RpB42AD pB42ADF pB42ADRpBACPAK8 BAC1 BAC2pBK-CMV T7 T3PBS(SK/KS)/M13- M13F/T7 T3/M13RpBV220 PBV220F PBV220RpCAMBIA 1301(1300) P1 P2pCAMBIA 2300 M13R(-48) M13F(-47)PCANTB5E S1/M13R S6pCAT3-enhancer RVP3 此载体无反向引物pcDNA3.0 CMV-F/T7 SP6/BGHpcDNA3.1 T7 BGHpcDNA4 T7/CMV-F BGHpcDNA6 T7/CMV-F(J21025在T7前面〕 BGHpcDNAII T7 SP6pCE2.1 M13F M13RpCEP4 pCEP-F EBV-RpCF-T M13F M13RpCI T7〔17Base〕此载体无反向引物pCI-neo T7〔17Base〕 T3pCMS-EGFP T7 T3pCMV-3Tag-4A T3 /PFLAG-CMV-F T7pCMV5 pCMV5F pCMV5RpCMV5-Flag pCMV5F pCMV5RpCMV-MYC/HA pCMV-F pCMV-RpCMV-Sport M13F/T7 SP6/M13RpCMV-Tag(KAN+) T3 T7pCR2.1-TOPO M13F/T7 M13RpCR3.1 T7 BGHpCS2 SP6 T7pDonar M13F M13RpDONR221 M13F M13RpDrive T7 SP6pDRIveR(KAN+) T7 SP6pDsRED1-C1 pDsRED-ex-C1-F pEGFP-N-3’(距离很近,一般不用) pDsRED2-C1(KAN+) pDsRED-ex-C1-F pEGFP-N-3’pDSRED-N1(KAN+) pEGFP-N-5’ PDSRED-N-RpECFP-C pEGFP-C-5’ pECFP-C-3'pECFP-N1 pEGFP-N-5’ pEGFP-N-3’pEF/myc/ER pEF-F pCDNA3.1RpEGFP-C(KAN+) pEGFP-C-5’ pEGFP-C-3'pEGFP-N(KAN+) pEGFP-N-5’ pEGFP-N-3’pENTR/D-TOPO(KAN+) M13F M13RpET-*(His)(KAN+) T7 T7 TerpET22(a,b,c)(AMP+) T7 T7 TerpET28,30,24,49(KAN+) T7 T7 TerpET32(a,b,c)(AMP+) T7/S.tag T7 TerpET50(amp+) T7/s.tag T7TERpET44(AMP+) T7/s.tag T7 TerpEYFP-N1 pEGFP-N-5’ pEGFP-N-3’pEYFP-C1 pEGFP-C-5’ pEGFP-C-3'pFLAG-CMV pFLAG-CMV -F pFLAG-CMV -RpGAD424 GAL4 AD 3'ADpGADGH GAL4 AD 3'ADpGADT7-Rec GAL4 AD/T7 3'ADpGAPZa-A a-FACTOR 3'AOXpGBKT7(Kana+) T7 3'BDpGEM-T(-Easy) M13F/T7 SP6/M13RpGEX-(*)T pGEX-4T-5’ pGEX-4T-3’pGL2 GLP1 GLP2pGL3 RVP3 GLP2(RVP4)(没有特别说明用GLP2) PinpointTM Vector Pinpoint Primer SP6pIRES PIRES-F/T7 T3/PIRES-R (J40720)’’pLXSN pLXSN-F pLXSN-RpMAL-c2E MalE primer M13F(-47)pMAL-C2x P5’ P3’pMAL-p2X MalE primer M13F(-47)PMD18-T M13R(-48) M13F(-47)PMD19-T M13F(-47) M13R(-48)pPIC9K(AMP+、ZEO+〕 5’AOX/a-factor 3’AOX pPICZa 5'AOX/PPICZa-F 3'AOXpPROEXHTA M13R(-48) 无反向引物pQE30or40 pQE30-F pQE-R:pRSET T7 T7TERpSilencer3.1-H1 hygro M13F(-47) 3.0REVpSILENCEV2.0-U6 T7 2.0REVpSK01-T M13F M13RpSK-CMV(KAN+) T7 T3pSP72 T7 SP6pSport1 SP6/M13F(-47) T7/M13RpSUPER T7 T3pTA2 M13F/T7(优先使用M13F〕 T3/M13RpT-Adv M13F /T7 M13RpTARGET TM pTarget.F(在T7前面〕/T7 pTarget.R pTO-T7 T7 T7TERpTRC99a-c pTRC99C-F pTRC99C-RpTriPLEx2 5'pTriPLEx2 T7pTWIN-1 T7 T7TER(客户确认再用〕pUC18(19)/118(119) M13F(-47) M13R(-48)pUCm-T M13F/T7 M13RpVAX1 T7 BGHpWSK29 T7/M13F/M13F(-47) T3/M13R/M13R(-48)pXT7 T7 SP6pYES2 T7 pYes2.RpLEXA pLEXA-F pLEXA-RpLNCX pLNCX-F pLNCX-RpRECEIVER CMV-F 此载体无反向引物pSHUTTLE-CMV PSHUTTLE-CMV-F PSHUTTLE-CMV-RpSP64/65 SP6 此载体无反向引物pSTBlue-1 T7 SP6pT7Blue(R) T7 M13F(-47)引物名称序列(5'-3')：M13R：CAG GAA ACA GCT ATG ACC。
常用载体及相关通用引物
常用载体及相关通用引物默认分类2008-10-06 13:16:31 阅读512 评论0 字号：大中小载体一端另一端pBlueScriptSK(+) M13F(-77)/M13F(-47)/M13F/T7 M13R(-88)/M13R(-48)/M13R/T3 pcDNA3 H1.3F/pCMV-F /pEGFP-N-5/T7 BGHpcDNA3.1(+) H1.3F/pCMV-F /pEGFP-N-5/T7 pCDNA3.1R/BGHpcDNA3.0(+)/myc-His C/A/B H1.3F/pCMV-F /pEGFP-N-5/T7 BGHpcDNA3.1(+)/myc-His C/A/B H1.3F/pCMV-F /pEGFP-N-5/T7 pCDNA3.1R/BGHpcDNA3.1(+)/myc-His/LacZ H1.3F/pCMV-F /pEGFP-N-5/T7 pCDNA3.1R/BGHpcDNA3.1/His C/A/B H1.3F/pCMV-F /pEGFP-N-5/T7 pCDNA3.1R/BGHpcDNA3.1/His/LacZ H1.3F/pCMV-F /pEGFP-N-5/T7 pCDNA3.1R/BGHpcDNA3.1/Hygro(-) H1.3F/pCMV-F /pEGFP-N-5/T7 pCDNA3.1R/BGHpcDNA3.1/Hygro(+) H1.3F/pCMV-F /pEGFP-N-5/T7 pCDNA3.1R/BGHpcDNA3.1/Hygro/LacZ H1.3F/pCMV-F /pEGFP-N-5/T7 pCDNA3.1R/BGHpcDNA3.1/V5-His C/A/B H1.3F/pCMV-F /pEGFP-N-5/T7 pCDNA3.1R/BGHpcDNA3.1/V5-His/LacZ H1.3F/pCMV-F /pEGFP-N-5/T7 pCDNA3.1R/BGHpcDNA3.1/V5-His-TOPO H1.3F/pCMV-F /pEGFP-N-5/T7 pCDNA3.1R/BGHpcDNA3.1/V5-His-TOPO/LacZ H1.3F/pCMV-F /pEGFP-N-5/T7 pCDNA3.1R/BGHpcDNA3.1/Zeo(-) H1.3F/pCMV-F /pEGFP-N-5/T7 pCDNA3.1R/BGHpcDNA3.1/Zeo(+) H1.3F/pCMV-F /pEGFP-N-5/T7 pCDNA3.1R/BGHpcDNA3.1/Zeo/CAT H1.3F/pCMV-F /pEGFP-N-5/T7 pCDNA3.1R/BGHpColdI DNA p-GEX3’pColdII DNA p-GEX3’pColdIII DNA p-GEX3’pColdIV DNA p-GEX3’pDream2.1 H1.3F/pCMV-F /pEGFP-N-5/T7 SP6pEGFP-N1,2,3 H1.3F/pCMV-F /pEGFP-N-5 pEGFP-N-3’/pEGFP-C-5’/pEGFP-C-3' pET15b T7 T7terpET20b T7 T7terpET22b T7 T7terpET28a T7 T7terpET28b T7 T7terpET30b T7 T7terpFastBac Dual pEGFP-C-3'pFastBac HT A,B,C pEGFP-C-3'pFastBac1 pEGFP-C-3'pGEM-T Easy M13F(-77)/M13F(-47)/M13F/T7 M13R(-88)/M13R(-48)/M13RpGEX-4T-1/3 p-GEX5’ p-GEX3’pGS-21a p-GEX5’ T7terpLenti6/V5-D-TOPO M13F(-47)/M13F/T7 M13R(-48)/M13R/T3pLenti6/V5-GW/lacZ M13F(-47)/M13F/T7 M13R(-48)/M13R/T3pMAL-p2x M13F(-47)/M13F/PBV220R M13R(-48)pMD18-T M13F(-77)/M13F(-47)/M13F M13R(-88)/M13R(-48)/M13RpMD19-T M13F(-77)/M13F(-47)/M13F M13R(-88)/M13R(-48)/M13RpMTBiPV5-His GFP p-GEX3’/ M13F(-47)/M13F M13R(-88)/M13R(-48)/M13R /pEGFP-C-3'/BGH pPCR script M13F(-77)/M13F(-47)/M13F/T7 M13R(-88)/M13R(-48)/M13R/T3pPIC3.5 5’AOX 3’AOXpPIC9 5’AOX 3’AOXpPICZ C/A/B 5’AOX 3’AOXpPICZalpha C/A/B 5’AOX/α-Factor 3’AOXpSecTag2 A,B,C H1.3F/pCMV-F /pEGFP-N-5/T7 BGHpTWIN T7 T7terpUC57 M13F(-77)/M13F(-47)/M13F M13R(-88)/M13R(-48)/M13RpUC18 M13F(-47)/M13F M13R(-48)/M13RpUC19 M13F(-47)/M13F M13R(-48)/M13R其他…… ……注：1）排列顺序为：有左到右==（离插入为点）由远到近；2）若有多个引物，红色的为首选引物：3）选择测序通用引物时，以距离插入位点约50碱基为佳；4）同时有T7，T7ter的载体，单反应首选T7ter；5）同时有T7，pCDNA3.1R的载体，单反应首选pCDNA3.1R ；6）同时有T7，BGH的载体，单反应首选BGH；7）同时有pCMV-F，BGH的载体，单反应首选BGH；。
通用引物_??????
通用引物
通用引物是一种在分子生物学中广泛应用的引物，用于扩增特定目标序列。
通用引物可以被设计成能够结合到多个不同物种的目标序列上，因此被称为“通用”。
以下是一些常见的通用引物：
1. 通用PCR引物：
- Forward引物：5' - GTGCCAGCMGCCGCGGTAA - 3'
- Reverse引物：5' - GGACTACHVGGGTWTCTAAT - 3'
2. 通用寡核苷酸引物（oligo-dT）：
- 5' - AAGCAGTGGTATCAACGCAGAGTAC(T)18 - 3'
3. 通用测序引物：
- Forward引物：5' - GTGCCAGCMGCCGCGGTAA - 3'
- Reverse引物：5' - GGACTACHVGGGTWTCTAAT - 3'
这些通用引物设计得具有较高的特异性和亲和力，可以在各种不同的生物种类中扩增目标序列，例如基因组DNA、cDNA或特定的基因片段。
但是，由于不同物种的基因序列差异和变异，通用引物在不同物种中的扩增效率和特异性可能会有所差异，因此在实验设计和优化过程中需要注意选择合适的引物。
测序通用引物序列
测序通用引物序列常见载体的测序引物：Primer of Vector:Vector：Primer(F)；Primer(R)pACT T7 T3pACT2 GAL4 AD pACT2-RpAS2-1 GAL4 BD pAS2-1.RpB42AD pB42ADF pB42ADRpBACPAK8 BAC1 BAC2pBK-CMV T7 T3PBS(SK/KS)/M13- M13F/T7 T3/M13RpBV220 PBV220F PBV220RpCAMBIA 1301(1300) P1 P2pCAMBIA 2300 M13R(-48) M13F(-47)PCANTB5E S1/M13R S6pCAT3-enhancer RVP3 此载体无反向引物pcDNA3.0 CMV-F/T7 SP6/BGHpcDNA3.1 T7 BGHpcDNA4 T7/CMV-F BGHpcDNA6 T7/CMV-F(J21025在T7前面）BGHpcDNAII T7 SP6pCE2.1 M13F M13RpCEP4 pCEP-F EBV-RpCF-T M13F M13RpCI T7（17Base）此载体无反向引物pCI-neo T7（17Base）T3pCMS-EGFP T7 T3pCMV-3Tag-4A T3 /PFLAG-CMV-F T7pCMV5 pCMV5F pCMV5RpCMV5-Flag pCMV5F pCMV5RpCMV-MYC/HA pCMV-F pCMV-RpCMV-Sport M13F/T7 SP6/M13RpCMV-Tag(KAN+) T3 T7pCR2.1-TOPO M13F/T7 M13RpCR3.1 T7 BGHpCS2 SP6 T7pDonar M13F M13RpDONR221 M13F M13RpDrive T7 SP6pDRIveR(KAN+) T7 SP6pDsRED1-C1 pDsRED-ex-C1-F pEGFP-N-3’(距离很近,一般不用) pDsRED2-C1(KAN+) pDsRED-ex-C1-F pEGFP-N-3’pDSRED-N1(KAN+) pEGFP-N-5’ PDSRED-N-RpECFP-C pEGFP-C-5’ pECFP-C-3′pECFP-N1 pEGFP-N-5’ pEGFP-N-3’pEF/myc/ER pEF-F pCDNA3.1RpEGFP-C(KAN+) pEGFP-C-5’ pEGFP-C-3′pEGFP-N(KAN+) pEGFP-N-5’ pEGFP-N-3’pENTR/D-TOPO(KAN+) M13F M13RpET-*(His)(KAN+) T7 T7 TerpET22(a,b,c)(AMP+) T7 T7 TerpET28,30,24,49(KAN+) T7 T7 TerpET32(a,b,c)(AMP+) T7/S.tag T7 TerpET50(amp+) T7/s.tag T7TERpET44(AMP+) T7/s.tag T7 TerpEYFP-N1 pEGFP-N-5’ pEGFP-N-3’pEYFP-C1 pEGFP-C-5’ pEGFP-C-3′pFLAG-CMV pFLAG-CMV -F pFLAG-CMV -R pGAD424 GAL4 AD 3′ADpGADGH GAL4 AD 3′ADpGADT7-Rec GAL4 AD/T7 3′ADpGAPZa-A a-FACTOR 3′AOXpGBKT7(Kana+) T7 3′BDpGEM-T(-Easy) M13F/T7 SP6/M13RpGEX-(*)T pGEX-4T-5’ pGEX-4T-3’pGL2 GLP1 GLP2pGL3 RVP3 GLP2(RVP4)(没有特别说明用GLP2) PinpointTM Vector Pinpoint Primer SP6pIRES PIRES-F/T7 T3/PIRES-R (J40720)pIRES2-EGFP pIRES2-EGFP.P5’ pIRES2-EGFP.P3’pLXSN pLXSN-F pLXSN-RpMAL-c2E MalE primer M13F(-47)pMAL-C2x P5’ P3’pMAL-p2X MalE primer M13F(-47)PMD18-T M13R(-48) M13F(-47)PMD19-T M13F(-47) M13R(-48)pPIC9K(AMP+、ZEO+）5’AOX/a-factor 3’AOX pPICZa 5′AOX/PPICZa-F 3′AOXpPROEXHTA M13R(-48) 无反向引物pQE30or40 pQE30-F pQE-R:pRSET T7 T7TERpSilencer3.1-H1 hygro M13F(-47) 3.0REV pSILENCEV2.0-U6 T7 2.0REVpSK01-T M13F M13RpSK-CMV(KAN+) T7 T3pSP72 T7 SP6pSport1 SP6/M13F(-47) T7/M13RpSUPER T7 T3pTA2 M13F/T7(优先使用M13F）T3/M13RpT-Adv M13F /T7 M13RpTARGET TM pTarget.F(在T7前面）/T7 pTarget.R pTO-T7 T7 T7TERpTRC99a-c pTRC99C-F pTRC99C-RpTriPLEx2 5′pTriPLEx2 T7pTWIN-1 T7 T7TER(客户确认再用）pUC18(19)/118(119) M13F(-47) M13R(-48)pUCm-T M13F/T7 M13RpVAX1 T7 BGHpWSK29 T7/M13F/M13F(-47) T3/M13R/M13R(-48)pXT7 T7 SP6pYES2 T7 pYes2.RpLEXA pLEXA-F pLEXA-RpLNCX pLNCX-F pLNCX-RpRECEIVER CMV-F 此载体无反向引物pSHUTTLE-CMV PSHUTTLE-CMV-F PSHUTTLE-CMV-R pSP64/65 SP6 此载体无反向引物pSTBlue-1 T7 SP6pT7Blue(R) T7 M13F(-47)引物名称序列(5′-3′)：M13R：CAG GAA ACA GCT ATG ACCM13F：TGT AAA ACG ACG GCC AGTM13F(-47)：CGC CAG GGT TTT CCC AGT CAC GACM13R(-48)：AGC GGA TAA CAA TTT CAC ACA GGA M13(-96)：CCC TCA TAG TTA GCG TAA CGSP6：ATT TAG GTG ACA CTA TAGT7：TAA TAC GAC TCA CTA TAG GGT7 terminator：TGC TAG TTA TTG CTC AGC GGT3：ATT AAC CCT CAC TAA AGG GApGEX-4T-5′：GGG CTG GCA AGC CAC GTT TGG TG pGEX-4T-3′：CCG GGA GCT GCA TGT GTC AGA GG GLp1：TGT ATC TTA TGG TAC TGT AAC TGGLp2：CTT TAT GTT TTT GGC GTC TTC CARVp3：CTA GCA AAA TAG GCT GTC CCRVp4：GAC GAT AGT CAT GCC CCG CGpcDNA3.1R：TAG AAG GCA CAG TCG AGGPinPoint primer：CGT GAC GCG GTG CAG GGC G pCMV-F：TCT AAA AGC TGC GGA ATT GTpCMV-R：TCCAAACTCATCAATGTATCpTRC99C-F: TTG CGC CGA CAT CAT AACpTRC99C-R: CTGCGTTCTGATTTAATCTGpCEP-F: AGA GCT CGT TTA GTG AAC CGEBV-R : GTG GTT TGT CCA AAC TCA TCpIRES2-EGFP.P5’: GTA GGC GTG TAC GGT GGG AG pIRES2-EGFP.P3’: AAC GCA CAC CGG CCT TAT TC3′AD: AGA TGG TGC ACG ATG CAC AGCMV -F：CGC AAA TGG GCG GTA GGC GTGS1：CAA CGT GAA AAA ATT ATT ATT CGCS6：GTA AAT GAA TTT TCT GTA GTA GG5`AOX1：GAC TGG TTC CAA TTG ACA AGC3`AOX1：GCA AAT GGC ATT CTG ACA TCCα-Factor：TAC TAT TGC CAG CAT TGC TGCGAL4 AD：TAC CAC TAC AAT GGA TGpACT2-R：GTGCACGATGCACAGTTGAApB42ADF：CCA GCC TCT TGC TGA GTG GAG ATGpB42ADR：AAG CCG ACA ACC TTG ATT GGA GpEGFP-N-5’：TGG GAG GTC TAT ATA AGC AGA G pEGFP-N-3’：CGT CGC CGT CCA GCT CGA CCA G pEGFP-C-5’：CAT GGT CCT GCT GGA GTT CGT G pEGFP-C-3′ ：TAT GGC TGA TTA TGA TCA GTPBV220F：AAG AAG GGC AGC ATT CAA AGPBV220R：CTG CGT TCT GAT TTA ATC TGU6：ATG GAC TAT CAT ATG CTT ACC GTA2.0rev primer：AGG CGA TTA AGT TGG GTA3.0rev：GAG TTA GCT CAC TCA TTA GGCS.tag：GAA CGC CAG CAC ATG GAC5′TriplEx2：CTC CGA GAT CTG GAC GAG CRVP4：GAC GAT AGT CAT GCC CCG CGRVP3：CTA GCA AAA TAG GCT GTC CCpQE30-R：GTT CTG AGG TCA TTA CTG GpQE30-F：TGA GCG GAT AAC AAT TTC ACpEF-F：TCA AGC CTC AGA CAG TGG TTCpDSRED-N1-R：TGA AGC GCA TGA ACT CCT TG pDsRED-express-C1-F：TCC CAC AAC GAG GAC TAC AC pCMV5R：ATT ATA GAG GAC ACC TAG TCpCMV5F：TTC CAA AAT GTC GTA ATA ACP5′：TGC GTA CTG CGG TGA TCA ACP3′：CTG CAA GGC GAT TAA GTT GGBAC2：ACG CAC AGA ATC TAG CGC TTBAC1：AAC CAT CTC GCA AAT AAA TA3′BD：TAA GAG TCA CTT TAA AAT TTG TAT AC pTarget.F：CCA GGA TTT TCC CAG TCA CpTarget.R：GGC TTT ACA CTT TAT GCT TCT7(17base)：ACA TCC ACT TTG CCT TTC TCpYes2.R：TCG GTT AGA GCG GAT GTGGAL4 BD：TCA TCG GAA GAG AGT AGpAS2-1.R：AAG AGT TAC TCA AGA ACA AGA ApFlag-CMV-F: GGT AGG CGT GTA CGG TGGpFlag-CMV-R: GCA CTG GAG TGG CAA CTTpIRES-F: CTT ACT GAC ATC CAC TTT GCpIRES.R: CAC TGC ATT CTA GTT GTG GTP1：CCA GGC TTT ACA CTT TAT GCP2：GCG ATT AAG TTG GGT AAC GCpLEXA-F：CGT CAG CAG AGC TTC ACC ATpLEXA-R：TAA AAC CTA AGA GTC ACT TTpLNCX-F：AGC TCG TTT AGT GAA CCG TCA GAT CG pLNCX-R：ACC TAC AGG TGG GGT CTT TCA TTC CC pLXSN-F：CTT GAA CCT CCT CGT TCG ACpLXSN-R：GTT GCT GAC TAA TTG AGA TGpSHUTTLE-CMV-F：GGT CTA TAT AAG CAG AGG TG pSHUTTLE-CMV-R：GTG GTA TGG CTG ATT ATG ATC AG MalE Primer：GGT CGT CAG ACT GTC GAT GAA GCC。
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常用载体测序引物列表 载体名称 正向引物 反向引物
pACT T7 T3
pACT2 GAL4 AD(5’AD) 3'AD/pACT2-R PACYCDUET-1(MCSII) DuetUP2 T7TER
pAD/pl-DEST CMV-F
pAD-Track pAD-Track-F 无
pAS2-1 5’BD M13-26/48 PB42AD PB42ADF PB42ADR PBAD PBAD-F PBAD-R pBABE pBABE5’ pBABE3’ PBACPAK8 BAC1
BAC2 pBK-CMV T7/M13-20 T3/M13-26 PBS(SK/KS)/M13- M13F/T7
T3/M13R pBV220 PBV220F PBV220R pBI121
需根据酶切位点确认 需根据酶切位点确认 pCAMBIA 1301(1300)
需根据酶切位点确认 需根据酶切位点确认 pCAMBIA 2300 需根据酶切位点确认 需根据酶切位点确认
PCANTB5E S1/M13R S6
pCAT3-enhancer pGL3+
pcDNA3.0 pEGFP-N5/T7 SP6/BGH
pcDNA3.1 pEGFP-N5/T7 BGH
pcDNA4 T7/pEGFP-N5 BGH
pcDNA6 T7/pEGFP-N5 BGH
pcDNAII T7
SP6 pCE2.1 M13F
M13R pCEP4 pCMV5R/PCEP-F pEGFP-N5/EBV-R pCF-T M13F
M13R pCI
T7（17Base ） pCMV5R pCI-neo T7（17Base ） T3
pCMS-EGFP T7
T3 pCMV-3Tag-4A T3 /pFlag-CMV-F T7
pCMV5 pCMV5F pCMV5R pCMV5-Flag pCMV5F
pCMV5R pCMV-MYC/NUC pCMV5F
pCMV5R PCMV-HA pCMV5F
pCMV5R pCMV-Sport
SP6/M13R
pCMV-Tag T3
T7 pCMV-TNT T7/SP6
pCMV5R pCR2.1-TOPO M13F/T7
M13R pCR3.1 T7
BGH pCS2 SP6
T7
PDNR-LIB M13F M13R
pDonar M13F M13R pDONR201 pDONR-F pDONR-R pDONR221 M13F M13R
pDrive T7 SP6 pDRIveR T7 SP6
pDsRED1-C1 pDsRED-C1-F pECFP-C-3 pDsRed1-N1 pEGFP-N-5 RFP-Nrev
pDsRed-Monomer-N1 pEGFP-N-5 pDsRed1-N
pDsRED2-C1 pDsRED-ex-C1-F pEGFP-N-3’
pEGFP-N-5 pDsRed1-N pDSRED2-N1
pDSRED-N1 pEGFP-N-5’ PDSRED-N-R pEASY-BLUNT M13F/T7 M13R
Ter
pEASY-E1 T7 T7
pEASY-T1 M13F/T7 M13R
pEASY-T3 M13F/T7 M13R/SP6 pECFP-C pEGFP-C-5’ pECFP-C-3' pECFP-N1 pEGFP-N-5’ pEGFP-N-3’
pEF/myc/ER pEF-F pCDNA3.1R pEGFP-C pEGFP-C-5’ pEGFP-C-3' pEGFP-N pEGFP-N-5’ pEGFP-N-3’ pENTR/D-TOPO M13F M13R
Ter
pET-*(His) T7 T7
pET22(a,b,c) T7 T7
Ter
Ter
pET28,30,24,49 T7 T7
Ter
pET32(a,b,c) T7/S.tag T7
pET-42a (-b, -c)(+) Stag T7 Ter
pET50 T7/s.tag T7TER
pET44 T7/s.tag T7
Ter pEYFP-N1 pEGFP-N-5’ pEGFP-N-3’ PETBlue M13-20 PETBlueDOWN PETDUET-1(MCSI) PBRrevBam DuetDOMN1
PETDUET-1(MCSII) DuetUP2 T7TER
pEYFP-C1 pEGFP-C-5’ pEGFP-C-3' pEYFP-N1 pEGFP-N-5’ pEGFP-N-3’ pFastBac pFastBac-F pFastBac-R pFastBac HT_A,B,C pFastBac-PH pECFP-C-3 pFastBac1 pFastBac-PH pECFP-C-3 pFastBac Dual (MCSI) pFastBac-PH pECFP-C-3 pFLAG-CMV CMV30/pFLAG-CMV-F CMV-24/pFLAG-CMV-R
p3xFlag-CMV-7.1 CMV-F/CMV24 CMV30
pGAD424 5’AD 3'AD
pGADGH 5’AD 3'AD
pGADT7-Rec 5’AD/T7 3'AD
pGAPZa-A/B/C a-FACTOR 3'AOX
forward 3'AOX
pGAPZ-A/B/C pGAP
pGBKT7 T7 3'BD
pGEM-T(-Easy) M13F/T7 SP6/M13R
pGEX-(*)T pGEX-5’ pGEX-3’
pGL2 pCMV5R PGL3-
Pgl3-BASIC pGL3+ PGL3-
pGL3-Enhancer pGL3+ PGL3-
PinPoint TM PinPoint Primer SP6
pIRES PIRES-F/T7 T3/PIRES-R
pIRES2-EGFP pIRES2-EGFP.P5’ pIRES2-EGFP.P3’ pLenti6/v5-Dest pEGFP-N-5’
PLEXA PLEXA-F PLEXA-R PLNCX PLNCX-F PLNCX-R pLXSN pLXSN-F pLXSN-R
Pjc1-tac T7
pJet.1.21.Blunt T7
primer M13F(-47) pMAL-c2E MalE
P3/PMAL-C2X-R pMAL-C2x P5/PMAL-C2X-F
primer M13F(-47) pMAL-p2X MalE
PMD18-T M13R(-48) M13F(-47) PMD19-T M13F(-47) M13R(-48) pMIR-report M13F
PPC86 PPC86-F PPC86-R
3’AOX
pPIC9K 5’AOX/a-factor
3'AOX pPICZa 5'AOX/PPICZa-F
pPROEXHTA M13R(-48)
pQE30or40 pQE30+ pQE30- pRECEIVER CMV-F
pRSET T7 T7TER PRSFDUET-1(MCSI) DuetUP1 DuetDOWN1 PRSFDUET-1(MCSII) DuetUP2 T7TER
pShuttle-CMV pShuttle-CMV-F pShuttle-CMV-R PSILENCE-1.0-U6 T7 T3
pSilencer3.1-H1 hygro M13F(-47) 3.0rev
pSILENCEV2.0-U6 T7 2.0rev
pSK01-T M13F M13R
pSK-CMV T7 T3
Psos H1
PSP64/65 SP6
pSP72 T7 SP6
T7/M13R
pSport1 SP6/M13F(-47)
pSTBlue-1 T7 SP6
pSUPER T7 T3
pT7Blue(R) T7 M13F(-47)
pTA2 M13F/T7 T3/M13R
/T7 M13R
pT-Adv M13F
pTARGET TM pTarget-F/T7 arget-R
PTHIOHISA,B,C TRX-FORWARD TRX-REVERSE
pTO-T7 T7 T7TER
PBV220-/PTRC99C-R pTRC99a-c PQE30+/PTRC99C-F
pTriPLEx2 PT5/5'TriplEx2 T7
pTz57r/t M13+ M13-
pTWIN-1 T7 T7TER
pUC18(19)/118(119) M13F(-47) M13R(-48)
M13F(-47)/M13F pUC57 M13R(-48)/M13R
pUCm-T M13F/T7 M13R
pVAX1 T7 BGH
T3/M13R/M13R(-48) pWSK29 T7/M13F/M13F(-47)
pXT7 T7 SP6
pYES2 T7 pYes2.R。