YARS抗体使用说明
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Species Research Area Target Names Image
CSB-PA00465A0Rb
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
P54577
Recombinant Human Tyrosine--tRNA ligase, cytoplasmic protein (2-528AA)
Liquid
Non-conjugated
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
>95%, Protein G purified
IgG
Polyclonal
Tyrosine--tRNA ligase, cytoplasmic (EC 6.1.1.1) (Tyrosyl-tRNA synthetase) (TyrRS) [Cleaved into: Tyrosine--tRNA ligase, cytoplasmic, N-terminally processed], YARS
Human
Epigenetics and Nuclear Signaling
YARS
Immunofluorescence staining of HepG2 cells with CSB-PA00465A0Rb at 1:100, counterstained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA00465A0Rb at dilution of 1:100
Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA00465A0Rb at dilution of 1:100
2
Immunofluorescence staining of SH-SY5Y cells with CSB-PA00465A0Rb at 1:100, counterstained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Rabbit
Human
ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200
Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr)
YARS Antibody
Biblioteka Baidu
Product Code Storage Uniprot No. Immunogen Raised In Species Reactivity Tested Applications
Relevance
Form Conjugate Storage Buffer
Purification Method Isotype Clonality Alias
1
Western blot All lanes: YARS antibody at 2µg/ml + 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 60 kDa Observed band size: 60 kDa
CSB-PA00465A0Rb
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
P54577
Recombinant Human Tyrosine--tRNA ligase, cytoplasmic protein (2-528AA)
Liquid
Non-conjugated
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
>95%, Protein G purified
IgG
Polyclonal
Tyrosine--tRNA ligase, cytoplasmic (EC 6.1.1.1) (Tyrosyl-tRNA synthetase) (TyrRS) [Cleaved into: Tyrosine--tRNA ligase, cytoplasmic, N-terminally processed], YARS
Human
Epigenetics and Nuclear Signaling
YARS
Immunofluorescence staining of HepG2 cells with CSB-PA00465A0Rb at 1:100, counterstained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA00465A0Rb at dilution of 1:100
Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA00465A0Rb at dilution of 1:100
2
Immunofluorescence staining of SH-SY5Y cells with CSB-PA00465A0Rb at 1:100, counterstained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Rabbit
Human
ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200
Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr)
YARS Antibody
Biblioteka Baidu
Product Code Storage Uniprot No. Immunogen Raised In Species Reactivity Tested Applications
Relevance
Form Conjugate Storage Buffer
Purification Method Isotype Clonality Alias
1
Western blot All lanes: YARS antibody at 2µg/ml + 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 60 kDa Observed band size: 60 kDa