系统生物学-合成生物学与转录组学
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2010年5月20日 J. Craig Venter Institute (JCVI), a notfor-profit genomic research organization, published results today describing the successful construction of the first self-replicating, synthetic bacterial cell. The team synthesized the 1.08 million base pair(1080kb) chromosome of a modified Mycoplasma mycoides genome. The synthetic cell is called Mycoplasma mycoides JCVI-syn1.0 and is the proof of principle that genomes can be designed in the computer, chemically made in the laboratory and transplanted into a recipient cell to produce a new self-replicating cell controlled only by the synthetic genome.
原体 ） large colony (LC), virtually
free of protein, was transplanted into
Mycoplasma capricolum（山羊支原体）
cells by polyethylene glycol(PEG)mediated transformation. Cells selected for tetracycline resistance, carried by the M. mycoides LC chromosome, contain the complete donor genome and are free of detectable recipient genomic sequences. These cells that result from genome transplantation are phenotypically identical to the M. mycoides LC donor strain as judged by several criteria.
Genome transplantation in bacteria: changing one species to another
天然完整基因组种间转移:
As a step toward propagation of synthetic genomes, we completely replaced the genome of a bacterial cell with one from another species by transplanting a whole genome as naked DNA. Intact genomic DNA from Mycoplasma mycoides（蕈状支
Daniel G. Gibson,1 John I. Glass et al
We report the design, synthesis, and assembly of the 1.08–mega–base pair Mycoplasma mycoides JCVI-syn1.0 genome starting from digitized genome sequence information and its transplantation into a M. capricolum recipient cell to create new M. mycoides cells that are controlled only by the synthetic chromosome. The only DNA in the cells is the designed synthetic DNA sequence, including "watermark" sequences and other designed gene deletions and polymorphisms, and mutations acquired during the building process. The new cells have expected phenotypic properties and are capable of continuous selfreplication.
--- Science. 2007 Aug 3; 317:632-8
Biblioteka Baidu
合成基因组---Science论文
Science 2 July 2010: Vol. 329. no. 5987, pp. 52 - 56
Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome
人工生命—artificial life
2007年6月 美国科学家克雷格•文特尔和他领导的研究小组宣布， 他们首次实现了完整的基因组在物种间的移植，这一成功为首个 「人造生命」的降生奏响了序曲。
文特尔说，这次成功让他向着制造出首个「人造生命」又迈进一 步，他将在几个月内利用人工合成的基因组展开类似的移植试验， 实现科研史上零的突破。如果试验成功，文特尔就能宣布他造出 全球第一个「合成生命」形式。
This research will be published by Daniel Gibson et al in the May 20th edition of Science Express and will
appear in an upcoming print issue of Science.
原体 ） large colony (LC), virtually
free of protein, was transplanted into
Mycoplasma capricolum（山羊支原体）
cells by polyethylene glycol(PEG)mediated transformation. Cells selected for tetracycline resistance, carried by the M. mycoides LC chromosome, contain the complete donor genome and are free of detectable recipient genomic sequences. These cells that result from genome transplantation are phenotypically identical to the M. mycoides LC donor strain as judged by several criteria.
Genome transplantation in bacteria: changing one species to another
天然完整基因组种间转移:
As a step toward propagation of synthetic genomes, we completely replaced the genome of a bacterial cell with one from another species by transplanting a whole genome as naked DNA. Intact genomic DNA from Mycoplasma mycoides（蕈状支
Daniel G. Gibson,1 John I. Glass et al
We report the design, synthesis, and assembly of the 1.08–mega–base pair Mycoplasma mycoides JCVI-syn1.0 genome starting from digitized genome sequence information and its transplantation into a M. capricolum recipient cell to create new M. mycoides cells that are controlled only by the synthetic chromosome. The only DNA in the cells is the designed synthetic DNA sequence, including "watermark" sequences and other designed gene deletions and polymorphisms, and mutations acquired during the building process. The new cells have expected phenotypic properties and are capable of continuous selfreplication.
--- Science. 2007 Aug 3; 317:632-8
Biblioteka Baidu
合成基因组---Science论文
Science 2 July 2010: Vol. 329. no. 5987, pp. 52 - 56
Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome
人工生命—artificial life
2007年6月 美国科学家克雷格•文特尔和他领导的研究小组宣布， 他们首次实现了完整的基因组在物种间的移植，这一成功为首个 「人造生命」的降生奏响了序曲。
文特尔说，这次成功让他向着制造出首个「人造生命」又迈进一 步，他将在几个月内利用人工合成的基因组展开类似的移植试验， 实现科研史上零的突破。如果试验成功，文特尔就能宣布他造出 全球第一个「合成生命」形式。
This research will be published by Daniel Gibson et al in the May 20th edition of Science Express and will
appear in an upcoming print issue of Science.