肝癌小鼠脾脏免疫细胞的变化及其意义

were elevated on week 1 ，then declined on week 2. CD8 +T cells reduced on week 2 , and NK cells decreased on week
3. There was no any
change in the
percentage
•1980 •
李 宝 华 ，等 肝 癌 小 鼠 脾 脏 免 疫 细 胞 的 变 化 及 其 意 义
肝癌小鼠脾脏免疫细胞的变化及其意义
李宝华1 黄 娜 1 陈海燕1 张 澍 2 ，王佩俊2 ，杨 军 3 , 李宗芳2’4
The dynamic alteration of splenic
immune
cells and its significance
percentages of
positive immune
in
spleen increased，the
negative immune status
the spleen’ thus facilitated hepatoma growth.
【K ey words ] spleen，H 22，hepatoma，immune cells
of NKT cells. After splenectomy ,the percentage
that of CD8 +T cells increased
in
peripheral blood and
tumor tissue. The
tumor weight
mice had no
changes, whereas, the
a murine H22 or
The murine
H22
orthotopic hepatoma model was established. During
the
different stage
of tumo
ges of total CD3 +T cells , CD4 +T cells , CD8 +T cells , NK cells and NKT cells in spleen were examined by flow cytom­
search Center o f Biodiagnosis and Biotherapy /The Second Affiliated Hospital ^School o f Medicine ,Xia.n Jiaotong University ^Shaanxi Xicin
710004 ’China. 【A b s tra c t] O b je ctive : To observe the percentage changes of positive and negative immune cells in spleen’ and to
Modern Oncology 2 0 1 8 ,2 6 (1 3 ) ：1980 -1 9 8 5
【摘要】 目的: 观察小鼠H 2 2 细胞原位种植性肝癌模型中，脾脏正性免疫细胞和负性免疫细胞比例的变化， 并且观察脾脏切除后外周血和肿瘤组织免疫细胞比例的变化以及对肿瘤生长的影响。方法: 建 立 小 鼠 H22 细胞原位种植性肝癌模型，在荷瘤的不同时期，用流式细胞术检测脾脏中负性免疫细胞（MDSC、T r g ) 和正性 免疫细胞（总 CD3 +T 细胞、CD4+T 细胞、CD8 +T 细胞、N K 细胞、N K T 细胞）比例的变化。荷 瘤 1 w 后切除脾 脏 ，用流式细胞术检测外周血及肿瘤组织中免疫细胞比例的变化，并比较切脾前后肿瘤重量、腹水量、荷瘤小 鼠生存期的变化。 结果: 荷瘤小鼠脾脏MDSC细胞的比例一直高于正常组;T r g 细胞在荷瘤2 w 时显著升高; 总 CD3 +T 细胞 和 CD4 +T 细胞在荷瘤1 w 时显著升高,2 w 时显著下降; CD8 +T 细胞在荷瘤2 w 时明显下降; N K 细胞在荷瘤3 w 时明显下降; N K T 细胞无显著变化。脾脏切除后外周血和肿瘤组织M D SC 的比例下降， CD8 +T 细胞的比例升高; 肿瘤重量和荷瘤小鼠生存期无显著变化，腹水量在荷瘤2 w 时显著减少，腹水发生 率明显降低。结论: 小鼠肝脏种植H 2 2 细胞株后，脾脏中正性免疫细胞的比例下降，负性免疫细胞的比例升
of tumor - bearingmice were also compared after splenectomy. R esults: Compared to normal m ice，splenic MDSCs
were progressively higher in TB mice. Treg significantly increased on week 2. The total CD3 +T cells and CD4 +T cells
etry. Splenectomy was performed 1 w a te r tumor inoculation ’ then the percentage alterations of immunocytes in periph­
eral blood and tumor tissue were detected by flow cytometry. The tumor weight,the amount of ascites and the survival
amount
clusion ：W ith the development of murine
H22
reduced and that of negative
immune cells
and
the
incidence of ascites significantly
reduce
orthotopic hepatoma,the
detect the alteration of the immune status in peripheral
blood and tumor tissue
after
splenectomy. Mea
growth was compared
in
splenectomy
group and
control group in
Li Baohua1, Huang N a1,C Biblioteka Baiduen H aiyan1, Zhang Shu2, Wang Peijun2 , Yang Jun3 ,L i Zongfang2,4
1Core Research Laboratory; Department of Surgery， Cadre Ward;3Department o f Pathology; National & Local Joint Engineering Re­