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A reduced glutamine synthetase activity indicated that disturbances in the nitrogen assimilation occur earlier than it was previously thought under salt stress. This study was created with the focus on very fast metabolomic responses that ensue during the initiation period of salt stress,secondly metabolomic responses were not only investigated in the above ground tissue but also in the roots.
3.Information on metabolic changes at early stages of salt stress is scant, regardless of the fact that salinity reduces biomass and yield formation. This study reports for the first that the proline analogue trans-4-hydroxy-L-proline that is known to inhibit cell elongation growth (Cleland, 1967)increased 60 min after NaCl stress initiation. 4.The reduced glutamine synthetase activity together with an accumulation of glutamate indicate that disturbances in the nitrogen assimilation occur earlier than it was previously thought under salt stress.
3.Metabolite extraction
4.GC-MS analysis of metabolites
5.Statistic evaluation of metabolite profile(PCA)
6.Photosynthesis rate
7.Sodium and chloride concentration
Fig 7
THANKS
L-2-aminoadipate,L-alanine,L-aspartate,L-methionine,L-proline, L-serine,melibiose,pyruvate,spermidine,urea,glycerol-3phosphate,trans-4-hydroxy-L-proline.
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Table 1
Fig 3
Fig 4
Fig 5
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Fig 6
Discussion
1. Fast responses of metabolites to salinity are different from long term responses. 2. Changes in leaf metabolites associated with the generation and scavenging of reactive oxygen species (ROS) together with the oxidation of the fluorogenic ROS sensor OxyBURST Green H2HFF suggest excessive ROS formation in this early phase.
8.Monitoring reactive oxygen species with
OxyBURST dye
9.Glutamine synthetase activity
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Fig1
Based on this PCA, the most relevant metabolites, 19 in number, were selected for a further individual analysis. They are:2oxoglutarate,Dgluconic acid,ethanolamine, glycine,inositol-1phosphate,isocitrate,
杨建宇 2015年10月23日
目 录
01 02 03 Introduction Methods Results
04
Discussion
Introduction
The novelty of this study is the demonstration of complex stress-induced metabolic changes at the very beginning of a moderate salt stress in V. faba （15-90min）, information that are very scant for this early stage. This study reports for the first that the proline analogue trans-4-hydroxy-L-proline, known to inhibit cell elongation, was increasingly synthesized after NaCl-stress initiation.
Fast responses of metabolites in Vicia faba L. to moderate NaCl stress
Christoph-Martin Geilfus Plant Physiology and Biochemistry 2014Impact Factor 2.756
Methods
1.Cultivation of plant material
Faba bean (V. faba L.) of the minor cultivar Scirocco (Saaten-Union GmbH , Isernhagen, Germany) were grown hydroponically in a climate chamber(14/10 h day/night; 20/15℃; 50/80% humidity).
Results
1.Metabolite responses in root and leaf tissue upon the initiation of the salt stress 2.Photosynthetic response 3.Leaf sodium and chloride concentration 4.ROS production 5.Glutamine synthetase activity
2.Experimental set up and sampling
Leaves (without petioles) and roots were sampled in liquid nitrogen before (0 min) and after (15, 30, 45, 60, 75, 90 min) the addition of 50 mM NaCl to the nutrient solution.