生物分离工程双语版2

生物分离工程双语版2
activity, higher conversion and higher lysisrate at longer time v D oerskovia systems high glucanase low protease activity,
contain some inhibitors v E an initial lag in yeast lysis by both enzyme v F simple two step model ( cell mass solubilized, protein
生物分离工程双语版2
1 Introduction
v Three factors of designing bioseparation processes
v (1) purity
v (2) cost
v (3) market
v Material state and choice of separation methods
v Structure (positive displacement pump, pistons, nozzle)
v Mechanisms
v (1) Shear
v (2) Cavitation （气穴）
v (3) impingement (冲击）
v Condition
v (1) concentration (4-175 g dry wt/liter)
v (2) pressure (30-95 MPa)
v (3) passes number (~5time)
v (4) modeled by equation
v (5) wild-type E. coli cells more difficult to disrupt (heat
induction at 42C)
PPT文档演模板
生物分离工程双语版2
•Ln(1/(1-R))=KNPΓ
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生物分离工程双语版2
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生物分离工程双语版2
Delineating mechanisms of cell disruption
v (1) facilitate controlling v (2) validating v (3) reproducible v (4) better design and optimize equipment v (5) Turbulent eddies (湍流涡旋）
生物分离工程-双语版2
PPT文档演模板
2020/11/26
生物分离工程双语版2
1 Introduction
v Considering protein properties for bioseparation
v (1) size
v (2) charge
v (3) biological affinity
understood v (3) sterilizable v (4) containable（兼容） and validatable v (5) amenable to being made automatic v (6) continuous and compact（紧凑的） v (7) economical
v Recombinant growth hormone
v Protein A and G.
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生物分离工程双语版2
Ideal large scale cell disrupter
v (1) disrupt tough organisms v (2) mechanism of cell disruption should be well
v Subdomain 亚域
v Domains 蛋白质结构的域
v Subunit 亚单位
v Endoplasmic reticulum 内质网
v Disulfide bond 二硫键
v Academia 学术界
v Delicate 脆弱的
v Flowchart 流程图
v Cytoplasmic 细胞质的
v Glyceraldehyde-3-phosphate dehydrogenase 甘油醛-3-磷酸 脱氢酶
v Reduced nicotinamide adenine 还原型尼克酰胺腺嘌呤
v Dinucleotide 二核苷
v Reductase 还原酶
v Hydrophobic 疏水的
PvPT文档H演模y板drophilic 亲水的
New words and vocabulary
v Amylase 淀粉酶
v Modulate 调节
v Interferons 干扰素
v Viscous fluids 粘性流体
v Signal peptide 信号肽
v Carboxypeptidase 羧肽酶
v Alkaline phosphatase 碱性磷酸酯酶
Responsible for cell disruption oscillate) v (6) shear stress, turbulence by v impingement
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生物分离工程双语版2
ຫໍສະໝຸດ Baidu
Enzymatic lysis cell disruption
v (1) a lytic protease (dissolve the outer part of the cell wall v (2) endo-β-(l-3) glucanase（葡聚糖酶） to disintergrate glucan net v (3) source（酶的来源） and culture system kinetic analysis v A understanding the system v B designing the small and large scale systems v C cytophaga （吞噬细胞）system exhibits high protease
v Toxin (enterotoxin)
v Subcellular components (mitochondrai, chloroplast)
v Intracellular constituents (DNA, RNA preparation, and
virus-like particles)
v Recombinant insulin
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生物分离工程双语版2
Products requiring cell disruption
v Vaccines (tetanus(破伤风）, meningitis（脑膜炎)
v Glucokinase（葡萄糖激酶）
v Glycerokinase （甘油激酶）
v Invertase （转化酶）
v Material secreted ( ultrafiltration, centrifugation)
v Not secreted material (cell disruption, solid-liquid separation)
v Material in liquid ( ultrafiltration, adsorption)
v (4) hydrophobicity-solubility
v Products from mammalian cell techniques
v (1) enzyme
v (2) hormones
v (3) nonantibody immunoregulators
v (4) monoclonal antibodies
v (2) easy downstream processing steps
v (3) not require cell disruption
v (4) avoid handling viscous fluids and proteinase
release
v (5) facility extraction of products
v (6) signal peptide determines secretion
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生物分离工程双语版2
Cell disruption
v (1) energy-intensive(强烈） and violent v (2) without destroying interest protein v (3) contain(兼容） process ( bacteria, v yeast, mammalian, plant, and insect v sources) v (4) be controlled v (5) be validated
hydrolyzed to peptides) v G Mishaelis-Menten kinetic equation predict cell wall lysis v H production of food grade single cell protein
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生物分离工程双语版2
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生物分离工程双语版2
Cell disruption goal
v Objective function (monetary（金钱） value of
v product)
v Small scale cell disruption
v (1) ultrasonic v (2) freeze thawing (熔化）
PPT文档演模板
生物分离工程双语版2
1 Introduction
v Initial bioseparation importance
v (1) filtration
v (2) centrifugation
v (3) chromatography
v Classified heuristics（论据，规则）
(subdomains, domains, and subunit assemblies) Second generation biotechnological products v (1) larger molecules v (2) more complex v (3) more glycosylate v (4) more sulphydryl bonds
v Periplasmic 外周胞质
v Osmotic 渗透
v urea 脲
v Guanidine 胍
生物分离工程双语版2
II Product excretion from the cell or cell disruption
v Excreted protein advantage
v (1) contamination level low
v (3) force cells through very small orifices at high
pressure
v (4) glass or porcelain ball mills
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生物分离工程双语版2
Large scale cell disruption
v Homogenizer
v (1) unit operation methods
v (2) design step sequence
v (3) species (components properties)
v (4) composition
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生物分离工程双语版2
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生物分离工程双语版2
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v Material in solid (extraction into aqueous solution)
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生物分离工程双语版2
PPT文档演模板
New and words
v Protacte lawsuits 诉讼
v Deleterious 有害的，有毒的
v Vexing 烦恼
v Hierarchic 分等级
(5) vPPT文档演模板 tumor specific antigens
生物分离工程双语版2
1 Introduction
v Protein folding v (1) short ranged interaction ( secondary structure ) v (2) gradual combination and reshuffling (重新组合）