DNA实验-免疫细胞表面抗原分子CD家族对照表(CD1-CD24

免疫细胞表面抗原分子CD家族对照表(CD1-CD247)

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Note:no information is available on CDw145, CD181, CD182, CD185-194, CD196-199, CD211, CD214-216,

CD218-220, CD237

Background

CD:cluster designation of monoclonal antibodies (clusters of differentiation)

Designated at the 1st to 7th Workshops on International Human Leukocyte Differentiation Antigens

The last conference was in 2000. The next conference is in 2004.

HLDA Workshops are the primary mechanism to characterize leukocyte surface antigenic molecules and epies; erythroid antigens are now included

For 1st to 6th workshops, antibodies were submitted to the organizing laboratory, coded and sent to participating laboratories for testing against various cell types. For the 7th workshop, a CD designation could be established for a molecule if its gene has been cloned and at least one specific monoclonal antibody had been studied in the Workshop

Interpretation should be based on cellular distribution of staining, proportion of positively stained cells, staining intensity and cutoff levels.

CD1

Family of non-polymorphic MHC class I-like glycoproteins

Also member of immunoglobulin superfamily

On chromosome 1q22-23 (not MHC linked)

Has 5 different subsets, all noncovalently associated with 12 kd beta 2 microglobulin

Function:restrict T cell responses to certain antigens; may mediate thymic T cell development

Positive staining (normal):cortical thymocytes (70%), activated T cells, Langerhans cells, interdigitating dendritic cells

Positive staining (disease):pre T ALL with cortical thymocyte phenotype; Langerhans cell histiocytosis

Negative staining:mature peripheral T cells

CD1a

Positive staining (normal):Dendritic cells in dermis/epidermis of benign inflammatory skin disorders

Positive staining (disease):Langerhans cell histiocytosis (fairly specific), myeloid leukemias, some B cell malignancies; dendritic cells in most peripheral cutaneous T cell lymphomas, AJCP 2001;116:72

Negative staining:normal B cells, most cutaneous peripheral B cell lymphomas (? reflects replacement of reactive pattern containing dendritic cells with a neoplastic pattern of B cells)

Micro images:Langerhans cell histiocytosis

Micro images (AJSP subscribers): pulmonary Langerhans cell histiocytosis

Micro images (Hum Path subscribers): Langerhans cell histiocytosis

References: AJSP 2001;25:630

CD1b

Positive staining (disease):myeloid leukemias and some B cell malignancies Negative staining:normal B cells