中国锹甲科分子系统发育研究(鞘翅目：金龟总科)-生态学专业毕业论文

中国锹甲科分子系统发育研究（鞘翅目：金龟总科）-生态学专业毕业论文

Abstract

Abstract

Lucanidae is one of the earliest branching lineages of Scarabaeoidea(Insecta：Coleoptera)．The common name of stag beetle is derived from the large and striking mandibles in males．Most of stag beetles have distinctly sexual dimorphism and male

and interspecific polymorphism，which usually caused the intraspecific differences

similarity．Many amateur entomologists often overlooked these traits and named numerous SO-called“new species and subspecies”．A lot of problematic genera and species need to be clarified in this family．In addition，there has been an argument

about the higher system of Lucanidae for a long time．

In this study,we tried to explore the phylogeny of Lucanidae using molecular systematic techniques．26 genera including 1 03 species(or subspecies)from Chinese fauna were analysed(mitochondrial COI，1 6S rDNA，nuclear册ngless and 28S rDNA) Totally over 500 sequences were amplified，sequenced and aligned of the four genes and other alternative genes．We conducted a phylogenetic analysis by using MP,ML and BI methods based on the combined sequences ofthe four genes．

As the results，the phylogeny tree exhibited a monophyly of Lucaninae．At the

subfamily level，the Syndesinae is recovered as paraphyletic．The genus of Sinodendron is the most primitive group in Lucanidae from Chinese fauna，which

should be treated as an independent subfamily Sinodendroninae．At the tribe level，the

Lucanidae tribes are Sinodendrini+ phylogenetic relationships among the ten Chinese

((Ceruchini+Lamprimini)+((Platycerini+Aesalini)+(Figulini+(Lucanini+

(Dorcini+(Odontolabini+AegiIli))))))．At the generic level，the genus Odontolabis is sister group of Neolucanus；the relationship of Pseudolucanus，Lucanus and Noseolucanus still need further research；the current genus of Prosopocoilus is a paraphyly,which is close relative to Rhaetulus，Hexthrius，Rhaetus，Pseudorhaetus and Dorcus 0．f．)．

Additionally,we sequenced，annotated and analyzed the mitochondrial genomes

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万方数据

中国锹甲科分子系统发育研究(鞘翅目：金龟总科)

of 8 species representing 7 genera of Lucanidae(6 completed mitochondrial genomes and 2 partial genomes)．The gene orders of most of them are identical to that of the putative ancestral arrangement of insects，while a trnL rearrangement occurred in the mtgenome ofSinodendronyunnanense．Three ofthem have a poly(Aft)stretch within their control regions．A phylogenetic analysis for 1 8 species of Scarabaeidae(others from Genbank)is conducted based on protein-coding genes．The main topological structures are consistent with the results obtained from the four genes．

Moreover,a time-calibrated phylogeny tree obtained from BEAST based Off four fossil data estimated the origin of crown group Lucanidae from China as circa 130million years ago(Mya)，and indicated that rapidly speciation occurred during

Oligocene．

Keywords：Molecular phylogeny,mtgenome，Lucanidae，divergence time

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万方数据