大豆籽粒异黄酮含量与IFS1基因相对表达量的关系_练云

分子植物育种，2014年，第12卷，第1期，第62－68页

Molecular Plant Breeding,2014,Vol.12,No.1,62－68

研究报告

Research Report

大豆籽粒异黄酮含量与IFS1基因相对表达量的关系

练云魏荷雷晨芳武永康李海朝王金社卢为国*

河南省农业科学院经济作物研究所,国家大豆改良中心郑州分中心,农业部黄淮海油料作物重点实验室,郑州,450002

*通讯作者,123bean@

摘要异黄酮合酶基因(isoflavone synthase,IFS)是合成异黄酮的关键酶基因之一，能够在转录水平对异黄酮的生物合成进行调控。为研究大豆籽粒中IFS基因与大豆异黄酮合成的关系，本研究选择8个不同地理来源的大豆品种，利用高效液相色谱法(HPLC)测定大豆籽粒中异黄酮4种主要组分含量(染料木素,大豆甙元,染料木甙和大豆甙)，并利用荧光定量PCR的方法测定了IFS1基因(IFS基因的同源基因之一)在大豆籽粒中的相对表达量。结果表明：大豆异黄酮4种主要组分含量在不同品种中差异较大，在所测试的品种中，中品03－5368和中豆27籽粒的总异黄酮含量较高，分别达到1873.136μg/g、1526.203μg/g，兴县灰皮支和Wiliams82总异黄酮含量较低，分别为1258.591μg/g和1255.300μg/g；大豆籽粒中大豆异黄酮总含量与籽粒中IFS1基因的相对表达量呈显著正相关(r=0.9955,P<0.0001)，大豆异黄酮4种主要成分中的染料木素和大豆甙与IFS1基因的相对表达量也成正相关，这一结果说明IFS1基因作为苯丙氨酸合成路径中进入异黄酮合成途径的第一个酶，在异黄酮化合物合成中起关键作用。本研究不仅可以为明确大豆异黄酮合成途径中IFS基因分子调控机理提供科学理论依据，而且能够为高异黄酮生物合成和大豆品质改良奠定理论基础。关键词大豆,大豆异黄酮,异黄酮合酶基因,高效液相色谱

Relationship between the Contents of Soybean Isoflavone and the Relative Expression Quantity of IFS1Gene in Soybean

Lian Yun Wei He Lei Chenfang Wu Yongkang Li Haichao Wang Jinshe Lu Weiguo*

Zhengzhou Subcenter of National Soybean Improvement Center,Key Laboratory of Oil Crops in Huanghuaihai Plains of Ministry of Agriculture,Insti-tute of Industrial Crops,Henan Academy of Agricultural Sciences,Zhengzhou,450002

*Corresponding author,123bean@

DOI:10.13271/j.mpb.012.000062

Abstract Isoflavone synthase(IFS)is one of the key enzymes involved in the synthesis of isoflavones,which participate in the regulation of isoflavone biosynthesis at the transcriptional level.In order to reveal the relat-ionship between the isoflavone content in soybean seed and the expression level of IFS1gene,eight soybean varieties,coming from different geographical origin,were selected to carry out the following experiments.The expression of IFS1was quantified by fluorescence quantitative PCR and the isoflavones components of four main content(Genistein,Daidzein,Genistin and Daidzin)were measured by high performance liquid chromatography (HPLC).The four main components of the isoflavones varied among different soybean varieties.Total isoflavone contents in Zhongpin03－5368and Zhongdou27was1873.136μg/g and1526.203μg/g,respectively.While the contents were as lower as1258.591μg/g and1255.300μg/g in Xingxian Huipizhi and Wiliams82,respectively. There was a significant positive correlation between the total contents of isoflavone(r=0.9955,P<0.0001), genistein,daidzin and the relative expression quantity of IFS1gene.It could be induced that IFS gene,as the first enzyme participating into isoflavones compounds synthesized,might play a key role in phenylalanine synthesis

收稿日期：2013－04－09接受日期：2013－05－21网络出版日期：2013－07－02

URL:/abstract－1598－mpbopa

基金项目：本研究由国家转基因生物新品种培育重大专项(2011ZX08004－003)和国家863项目(2012AA101106)共同资助