基因工程2

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Teamwork 1 scores / 团队竞赛1 得分表
One letter words Team 1 7 Team 2 8 Team 3 8 Team 4 6 Team 5 5 Team 6 8 Team 7 7 Team 8 10 ?
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A5 → Transcription can be terminated in two ways 转录能以两种方式终止
Intrinsic termination / 内在型终止
ρ-dependent termination / ρ依赖型终止
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Questions for Lecture 6
α σ
β’ β α
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A4 → Transcription errors can be corrected in two ways 有两种方式可以用来纠正转录错误
Pyrophosphorolytic editing 焦磷酸化编辑
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Hydrolytic editing 1 水解编辑（1）
Score 95 100 100 90 85 100 95 100
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Team members / 团队成员
Team 1 朱琳颖、潘薇、王芳、侯莎莎、张歌奕 Team 2 张琴、吴又琼、张静华、马红、郭春燕 Team 3 陈亚志、汪晓云、沈晓丹、曹婷婷、丁娣
Team 4 张若花、杨芳、赵宝林、李博中、张欣
Condition A
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Regulation of gene expression 基因表达调控
Condition B
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3.3.1 Coordinate Regulation 协同调控
Coordinate regulation: Transcriptional regulation in which a set of genes are regulated together.
Q1 → How can E. coli survive when there is only lactose ?
只有乳糖时大肠杆菌如何生存呢？
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Vocabulary of Lecture 6 (1/2)
gene expression coordinate regulation polycistronic mRNA cistron operon operator lactose galactose permease galactosidase
22 / 45 Glucose – Lactose –
E. coli: ―I will use lactose only when there is no glucose and there is lactose.‖
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Regulated Expression of Lactose Metabolizing Genes 乳糖代谢基因表达调控
Team 5 汪宇言、彭展鸿、单宝、任新龙、曹洋 Team 6 王泽、傅建军、朱楚楠、夏昌选、赵寒松 Team 7 王斌、唐懿骏、邢兵、陈杰、钱晨明
Team 8 蒋琛、孔培中、王鹏飞
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Lecture 6
Chapter 3 第3章 3.1 为什么使用RNA
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A2 → The player is RNA polymerase ! RNA聚合酶是玩家 !
α σ β’ β α
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A3 → Transcription is initiated at the promoter region. σ subunit of the RNA polymerase is responsible for recognizing the promoter. 转录是从启动子区域起始的，RNA聚合酶的 σ亚基负责识别启动子。
1. Negative Regulation – the lac Repressor 负调控──lac阻遏蛋白 2. Positive Regulation – CAP 正调控──CAP 代谢物激活蛋白
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3.1 Why Use an RNA Intermediate?
3.2 Mechanism of Transcription 3.3 Regulation of Gene Expression in Prokaryotes 3.3.1 Coordinate Regulation 3.3.2 The Lac Operon 3.3.3 The Trp Operon 3.3.4 Ara & Gal Operons
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Hydrolytic editing 2 水解编辑（2）
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乳糖渗透酶 转乙酰基酶
Β-半乳糖苷酶
E. coli: ―I need lactose permease, β-galactosidase, and transacetylase .‖ 21 / 45
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When to use lactose / 何时利用乳糖
Glucose + Lactose + Glucose – Lactose +
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3.3.2 The lac Operon / 乳糖操纵子
Lac operon: An operon containing genes involved in lactose metabolism. lac操纵子：含有乳糖 （lactose）代谢基因的 操纵子。
Promoter Operator
3.3.1 Coordinate Regulation
3.3.2 The Lac Operon 3.3.3 The Trp Operon 3.3.4 Ara & Gal Operons
3.3.4 阿拉伯糖与
半乳糖操纵子
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Regulation of gene expression 基因表达调控
Lactose 乳糖
CH2OH OH O O OH OH CH2OH O OH
OH OH OH
Glucose 葡萄糖
CH2OH O OH
OH
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E. coli: ―I do not usually eat lactose, but in case when there is no glucose . lacA
The lac operon
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