LAS-Trangenic animal

LDL-rich
Yes No Chylomicron Downregulated Sensitive Susceptible
LDL-rich
Yes No Chylomicron Downregulated ? ?
VEGF Transgenic
Creating a transgenic rabbit model
Yeast, bacterial and cultured cells harboring a foreign DNA fragment are known as recombinant or transformed cells. Transgenesis does not include the techniques of radiation, chemical or viral mutagenesis, selective breeding, nor does it includes gene therapy and animal cloning.
转基因动物的制作
How we can create transgenic animal
Pronuclear DNA microinjection Knock-out / Knock-in Timed and tissue-specific gene
targeting Other methods
The large size of rabbits facilitates many
biomedical studies
Comparison of lipoprotein metabolism characteristics between mouse, rabbit, and human
Mouse Rabbit Human
刘恩岐等. 动物学杂志. 2006；41:64-71
原核显微注射 Pronuclear DNA microinjection
Construction of vector (recombinant

DNA) Getting the zygote Microinjection of foreign DNA Embryo transfer Detection and maintenance of transgenic animal strain
Pronuclear DNA microinjection
Construction of vector (recombinant

DNA) Getting the zygote Microinjection of foreign DNA Embryo transfer Detection and maintenance of transgenic animal strain
VEGF Transgenic
Transgene of VEGF expression construct
Pronuclear DNA microinjection
Construction of vector (recombinant

DNA) Getting the zygote Microinjection of foreign DNA Embryo transfer Detection and maintenance of transgenic animal strain
It has not been defined whether VEGF
is a cause or consequence of these kidney disorders.
假设 Hypothesis
To examine whether increased VEGF in the kidney may directly participate in the process of glomerulopathies.
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03
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Zhao et al. Scand J Lab Anim Sci. 2007
Why we need transgenic animal What is transgenic animal How we can create transgenic animal Where we can use transgenic animal
Why we need transgenic animal What is transgenic animal How we can create transgenic animal Where we can use transgenic animal
血管内皮生长因子
(Vascular endothelial growth factor, VEGF)
Comparison of the two forms of transgenes
The genomic form includes all naturallyoccurring intron elements that are involved in mRNA splicing and expression, whereas the shorter cDNA form is a synthetic sequence representing only the protein-encoding exon elements of the gene.
overexpressing VEGF
Studying VEGF in the process of
glomerulopathy.
!!!
Why we need to transgenic animal What is transgenic animal How we can create transgenic animal Where we can use transgenic animal
In a broad sense, transgenic animals is used to refer to transgenic, knock-out, knock-in and knock-down animals.
Oct 18, 2007
Gene engineering animal 4,970,000 Genetic engineering animal 1, 480,000 Transgenic animal 1,630,000
转基因 Transgene
Transgene is a piece of foreign DNA that has become incorporated into the genome of a cell.
转基因技术 Transgenesis
Transgenesis implies that the intentional introduction of foreign genetic material into the genome of plants or animals.
One of the best experimental approaches is to use transgenic animals that overexpress VEGF.
VS.
Rabbits are a useful model for the study of
vascular biology
Schematic diagram of the double-stranded DNA regions of the transgene expression cassette.



Restriction enzyme recognition sites are clustered at either end of the cassette. ATG, the beginning of the transcriptional reading frame. SIG, the signal sequence. AAA, the poly-A tail
转基因动物 Transgenic Animal
Transgenic animals are animals which carrying and expressing a foreign DNA. Foreign DNA is introduced into the animal, using recombinant DNA technology, and then must be transmitted through the germ line so that every cell, including germ cells, of the animal contains the same modified genetic material.
转基因动物的制作
How we can create transgenic animal
Pronuclear DNA microinjection
Knock-out / Knock-in
Timed and tissue-specific gene
targeting Other methods
转基因动物 Transgenic animal, Where we are & where we will go
Liu Enqi, PhD Laboratory Animal Center Medical School, Xi’an Jiaotong University
Why we need transgenic animal What is transgenic animal How we can create transgenic animal Where we can use transgenic animal