各种荧光染料光谱数据

GFP light cube GFP light cube GFP light cube GFP light cube GFP light cube GFP light cube GFP light cube
Dye
GFP light cube 5-FAM (carboxyfluorescein) Acridine Orange Acridine Yellow Alexa Fluor 430™ Alexa Fluor 480™ Alexa Fluor 488™ Amplex Gold ATTO 465 Auramine Aurophosphine BCECF BODIPY 492/515 BODIPY 505/515 Bodipy FL BTC Calcein Calcium Green-1 Catskill Green 540 CellTracker Green CFDA CFP Cy2 cyQUANT GR DAF-FM DCF DiO Diversa Green-FP Emerald FITC Fluo-3 Fluo-4 Fluoro-Emerald Fluoro-Jade B Fluor-X FluoSpheres Yellow-Green GFP (EGFP) HCS LipidTox Green HPTS (Solvent Green 7) Live/Dead Fixable Green Lucifer Yellow LysoSensor Green
EVOS®fl FLUOROPHORE LIST
Other Cubes
(click to view)
GFP light cube RFP light cube Texas Red light cube Cy5 light cube CFP light cube YFP light cube
DAPI light cube DAPI light cube DAPI light cube DAPI light cube DAPI light cube DAPI light cube DAPI light cube
EM Peak
447/60 EM 480 440 430 449 445 462 442 421 425 442 448 440 451 440 420 525 455 450 554 440 412 487 483 442 424 422 460 410 461 455 420 430 461 450 460 449 460 483 425 435 442
EVOS LED Light Cube Compatibility REV 2 Published 20 JUL 2009
EVOS®fl FLUOROPHORE LIST
Other Cubes
(click to view)
DAPI light cube GFP light cube Texas Red light cube Cy5 light cube CFP light cube YFP light cube
ABS Peak
504 506 478 490 450 467 497 465 498 512 502 UV UV 496 507 497 496 498 479 484 488 474 506 498 469 495 497 507 506 487 490 452 482 503 504 520 515 513 505 475 512 491 491

ABS Peak
470/22 EX 492 500 470 432 490 500 512 453 460 470 499 490 502 502 464 494 506 482 490 494 434 492 500 493 504 489 484 490 492 506 494 494 480 494 501 488 498 455 498 423 448
Spectrum Match*
Excellent Excellent Excellent Excellent Excellent Excellent Excellent Excellent Excellent Excellent Excellent
Good Excellent Excellent
Good Fair Excellent Excellent Excellent Excellent Excellent Excellent Excellent Excellent Excellent Excellent Excellent Good Good Excellent Good Good Excellent Good Good Excellent Excellent Good Excellent Excellent Excellent
EM Peak
511 530 561 512 530 538 524 510 524 532 522 525 543 520 529 523 523 516 507 510 511 509 532 522 526 439 521 524 525 505 520 484 500 527 523 541 530 531 532 509 529 507 509
Spectrum Match*
Good Fair Excellent Excellent Excellent Excellent Excellent Excellent Good Fair Good Fair Fair Good Fair Good Good Good Good Good Good Excellent Good Good Excellent Good Good Good Fair Good Excellent Good Excellent Good Good Fair Fair Fair Good Excellent Fair Excellent Excellent
EVOS LED Light Cube Compatibility REV 2 Published 20 JUL 2009
EVOS®fl FLUOROPHORE LIST
Other Cubes
(click to view)
DAPI light cube RFP light cube Texas Red light cube Cy5 light cube CFP light cube YFP light cube
Spectrum Match*
Good Good Excellent Good Excellent Good Good Excellent Excellent Excellent Good Good Good Good Excellent Good Fair Excellent Excellent Good Fair Excellent Excellent Good Good Excellent Excellent Good Excellent Fair Good Good Excellent Good Good Excellent Fair Excellent Good Fair Excellent
EVOS LED Light Cube Compatibility REV 2 Published 20 JUL 2009
EVOS®fl FLUOROPHORE LIST
Other Cubes
(click to view)
DAPI light cube RFP light cube Texas Red light cube Cy5 light cube CFP light cube YFP light cube
Dye
DAPI light cube 1,8-ANS 4-methylumbelliferone 7-amino-4-methylcoumarin 7-hydroxy-4-methylcoumarin ABQ Acridine Alexa Fluor 350 Alexa Fluor 405 AMCA AMCA-X BFP Calcein Blue Calcein Violet Calcofluor White Cascade Blue Dansyl DAPI DiFMU (pH 9.0) ER-Tracker Blue-White DPX Fast Blue Fluospheres Blue Hoechst 33258 Hoechst 33342 Live/Dead Fixable Blue Dead Cell Stain LysoSensor Blue LysoTracker Blue Marina Blue Methoxycoumarin Monochlorobimane Pacific Blue PBFI Phorwite AR Quinine Sulfate sgBFP™ (Super Glow BFP) SITS SpectrumBlue® Thioflavin TCN Thiolyte True Blue Uvitex SFC Y66H

GFP light cube GFP light cube GFP light cube GFP light cube GFP light cube GFP light cube GFP light cube
Dye
LysoTracker Green Magnesium Green mHoneydew MitoTracker™ Green FM NBD Amine NBD-X Neurotrace 500/525 Green Nitrobenzoxadidole Oregon Green™ 488 Oregon Green™ 514 PicoGreen Qdot 525 Qdot 545 Rhodamine 110 Rhodamine 123 Rhodamine Green Rhodol Green rsGFP (red shifted GFP, S65T) S65C S65L S65T sgGFP™ (Super Glow GFP) Sodium Green SpectrumGreen® Surf Green EX SYBR Gold SYBR Green I SYBR Safe SYTO 11 SYTO 13 SYTO 16 SYTO 45 SYTO 9 SYTO RNASelect SYTOX Green TET TO-PRO-1 TOTO-1 Vibrant Dyecycle Green wtGFP (wild type GFP, non-UV excitation) YFP YO-PRO-1 YOYO-1

ABS Peak
357/44 EX 372 360 351 360 344 362 346 401 345 353 382 373 401 350 377 335 345 357 371 360 356 345 347 344 374 373 350 378 365 365 360
RFP light cube RFP light cube RFP light cube RFP light cube RFP light cube RFP light cube RFP light cube
Dye
RFP light cube Alexa Fluor 532 Alexa Fluor 546 Alexa Fluor 555 Alexa Fluor 568 Alexa Fluor 594 Amplex Red Amplex UltraRed BOBO-3 Bodipy R6G Bodipy TMR Bodipy TRX Calcium Orange Carboxy SNARF-1 Carboxyrhodamine 6G CellMask Orange CellTrace Calcein Red-Orange CellTracker Orange CellTracker Red Cy3 Cy3.5 DiI DsRed Monomer DsRed2 DsRed-Express dTomato Eosin Ethidium Bromide Ethidium Homodimer-1 Fluospheres Orange Fluospheres Red FocalCheck Double Orange FocalCheck Double Red HCS LipidTox Red HEX JOJO-1 Live/Dead Fixable Red LOLO-1 Lysotracker Red MitoTracker Orange MitoTracker Red
EM Peak
525/50 EM 518 526 550 540 510 540 540 507 540 540 540 540 540 540 540 540 540 540 540 540 540 540 540 540 540 540 507 540 520 540 540 518 525 520 513 507 506 510 526 543 503