1.串联液质系统分析原理

串联液质系统分析原理
美国应用生物系统中国公司 应用技术服务部
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What is a mass spectrometer ?
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Date | AB CONFIDENTIAL
© 2006 Applied Biosystems
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质谱技术：
● 质谱分析过程：样品通过进样系统进入离子源，由于结构 性质不同而电离为各种不同质荷比（m/z）的分子离子和碎 片离子，而后，带有样品信息的离子碎片被加速进入质量 分析器，不同的离子在质量分析器中被分离并按质荷比大 小依次抵达检测器，经纪录即得到按不同质荷比排列的离 子质量谱，也就是质谱 (mass spectrum)。 ● 质谱：按离子的质荷比的大小依次排列形成的图谱
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Date | AB CONFIDENTIAL
© 2006 Applied Biosystems
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质谱仪的主要部件： 真空系统 数据系统
样品导入 系统
直接进样 GC LC CE
离子源
质量分析器
离子检测器
MALDI ESI API APCI APPI
四极杆：Quadrupole 离子阱、线性离子阱：IT，LIT 飞行时间：TOF 傅立叶变换离子回旋共振：FT-ICR
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Date | AB CONFIDENTIAL
© 2006 Applied Biosystems
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高效分离系统
高灵敏度检测系统
GC 大量的载气
EI、CI…
MS 高真空体系
HPLC 大量的液态流动相
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MS/MS 高真空体系
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Date | AB CONFIDENTIAL
© 2006 Applied Biosystems
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液相色谱与质谱的联用技术
● 主要障碍：
1、能否把大量的溶剂除去并不使质谱仪受到污染 2、能否使被分析样品在温和条件下带上电荷得到分析而 不被分解
上面两点是影响灵敏度的主要因素
GS2 TEM
GS1 GS2 TEM
1、GS2↑，TEM↓ 2、H2O比例大时，TEM ↑，有利于离子化
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Date | AB CONFIDENTIAL
© 2006 Applied Biosystems
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Triple Quadrupole sequence of events
Ion production Ion transport Ion filtering Ion processing Ion filtering Ion detection
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Date | AB CONFIDENTIAL
© 2006 Applied Biosystems
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1st Step：Ion Production
Ion production
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Principle of Electrospray (ESI)
IonSpray™ source: Electrospray Ionization (ESI) softest ionization technique polar to ionic substances
11 Date | AB CONFIDENTIAL © 2006 Applied Biosystems
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Principle of Atmospheric Pressure Chemical Ionization (APCI)
Heated Nebulizer source: Atmospheric Pressure Chemical Ionization (APCI) corona discharge needle polar to unpolar and thermally stable compounds
12 Date | AB CONFIDENTIAL © 2006 Applied Biosystems
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2nd Step：Ion transport
Ion transport
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Date | AB CONFIDENTIAL
© 2006 Applied Biosystems
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Curtain Gas™ interface
ATMOSPHERIC PRESSURE
CUR DP SK Q0 + + FP + + + + + + +
VACUUM
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N2
1. Assists in droplet evaporation 2. Declusters ions – CID (in-source) fragmentation 3. Collisional focussing in Q0
14 Date | AB CONFIDENTIAL © 2006 Applied Biosystems
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