激素性股骨头坏死病程中股骨头局部

・实验研究・

激素性股骨头坏死病程中股骨头局部

V EGF mRNA 表达的变化及意义

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樊粤光　徐传毅　何　伟　方　斌　李　雄　刘少军　曾意荣　袁　浩

摘　要　目的:探讨激素性股骨头坏死病程中股骨头局部血管内皮生长因子(vascular endothelial growth factor ,

V EGF )mRNA 表达的变化及意义。方法:新西兰大白兔36只,随机均分成正常对照组和模型组,每组18只。模型组用

内毒素加激素注射造成股骨头坏死。两组动物分别于造模后6、10、14周各处死6只行股骨头组织学检查和V EGFmR 2

NA 原位杂交观察。结果:光镜观察示模型组股骨头骨小梁变细、空骨陷窝增加,成骨细胞数量减少,这些表现随着时间

的推移逐渐加重;软骨下血窦计数减少,各时段均低于正常组,差异有显著性意义(P <0.05,或P <0.01)。原位杂交显示,模型组兔的股骨头6周时血管周围有V EGFmRNA 阳性表达,10周时血管、成骨细胞的表达逐渐减少,14周时稍有所增加。结论:股骨头坏死后血管修复能力下降,血管内皮细胞的功能受到破坏影响,增加股骨头局部V EGF 可能有助于股骨头坏死的修复。

关键词　股骨头坏死;　血管内皮生长因子;　原位杂交

中图分类号　R681 文献标识码　A 文章编号　1005-8478(2002)08-0784-03

Change and Its Signif icance of the Expression of VEGF Messenger RNA of the Femoral H ead in the Steroid 2induced Avascular N ecrosis of Femoral H ead ∥FA N Y ue 2guang ,XU Chuan 2yi ,HE Wei ,et al.The f irst A f f iliated Hospital of Guangz hou U niversity of T raditional Chinese Medicine ,Guangz hou 510405

Abstract Objective :To explore the change and its significance of the expression of vascular endothelial growth factor (V EGF )messenger RNA of the femoral head in the steroid 2induced avascular necrosis of femoral head (ANFH ).Method :36New Zealand rabbits were randomly and equally divided into normal control group and model group.18rabbits in model group were injected endotoxin and methylprednisolone.6rabbits were killed at the time of the sixth week ,tenth week ,fourteenth week in each group respectively.Femoral head s pecimens were studied by histological examination and in situ hybridization.Results :By light microscopy ,empty lacunae increased and numbers of osteoblasts and subchondral sinosoids decreased in model group.By in situ hybridization ,expression of V EGF mRNA showed decrease in the osteoblasts and vascular endothelial cells in the femoral head of model group in the sixth week.Expression decased in the tenth week and increased slightly in the fourteenth week.Con 2clusior :The ability of blood vessel reparation dereases during avascular necrosis of the femoral head.Function of endothelial cells suffers the interruption as well.Increasing local V EGF in the femoral head may be beneficial to the reparative process of necrosis of the femoral head.

K ey w ords Avascular necrosis of the femoral head ;　Vascular endothelial growth factor ;　In situ hybridization

△广东省自然科学基金项目(990367)

　教育部高校骨干教师重点资助项目(200055)

作者单位:广州中医药大学第一附属医院髋中心,广州510405作者简介:樊粤光(19542),男,山西籍,教授,主任医师。研究方向:

髋、膝关节疾病。电话:(020)36591348

在组织的发育、改建和修复的过程中,均有生长因子的参与和作用,特别在骨骼的形成、骨折的愈合与骨骼的改建过程中。在股骨头坏死修复与重建中,血管与骨的新生是一个病理逆转的关键。而骨的新生又离不开血管的新生,已知的与血管新生有关的生长因子有碱性成纤维细胞生长因子

(bF GF )、转化因子β(TGF -β)、血管内皮生长因子(V EGF )等,其中V EGF 特异作用于内皮细胞,促进其增殖和血管生成,并可增加血管通透性,也可能在骨的形成与改建中发挥重要作用〔1〕。本研究用原位杂交法,观察激素性股骨头坏死病程中股骨头局部V EGFmRNA 表达的变化及意义,旨在深入探讨股骨头坏死的病理机制。

1　材料与方法

1.1　实验材料1.1.1　实验动物

健康成年新西兰大白兔36只,雌雄各半,体重(2.0±0.5)

kg ,由广东省医学实验动物中心提供。1.1.2　试剂盒