荧光探针和分子传感器

3566 篇 206 篇 1890 篇 120 篇
研究与应用领域涉及：生命 环境 材料 等
讲座要求了解： 荧光 探针 表征 应用 研究意义
Molecular Probe
Molecular reporter
From J. Fluorescence
Molecuhloamr perpoabgees www. probes. com
郭祥群博士，博士生导师
全国高校化学教育研究中心第四届学术委员会副主任， 教育部高等学校本科教学工作水平评估专家，中国化 学会理事
先后获得国家级奖2项，省部级奖4项，发表科研与 教学研究论文100余篇。
研究兴趣：荧光光谱分析的原理与技术，在研项目涉 及生物活性自由基的荧光表征，海洋污染生物监测技 术研究，食品安全等。
decay rate
Expression
Average lifetime: the average time the molecule spends in the excited state prior to return to the ground state.
1
Γ knr
Fluorescence Quantum Yield
Jablonski diagram
S2
Vibrational relax, VR, 10-1210-14 s
S1
LUMO
Absorption 10-15 s
hA hA
S0
HOMO
Internal conversion, IC, 10-1110-13 s
Intersystem crossing, ISC, 10-210-6 s
Stoke’s shift
Fluorescence lifetime 荧光寿命 Fluorescence quantum yield 荧光量子产率
Fluorescence Anisotropy 各项异性 r Fluorescence Polarization 荧光偏振 p
Excitation spectrum em
S
R
T
+
自由基传感
Z.-Y. Bian, X.-Q. Guo*, Analytical Science, 21 553-559, 2005
Sensor (2)
hu hv e - R S
D
T hv
hv
D
PET Photoinduced Electron Transfer
Hg2+ hv
E. M. Nolan, et al., J. Am. Chem. Soc., 2003, 125: 14270-14271
Definition
The fluorescence quantum yield is the ratio of the number of photons emitted to the number absorbed
Expression
Φ Γ Γ knr
Sensor (1)
S 信号单元， R 识别单元， T 靶分子
F
Emission spectra
ex
F
1
ex
em
Fluorescence lifetime
Simplified Jablonski diagram
S1
hvA S0
Definition
S1 hvF
relaxation (10-12 s)
: Emission rate
knr Knr: nonradiative
膜 鼠细胞
内
蛋
白
侧
向
运
37°C
动
融合
罗丹明标记 人蛋白质 人细胞
异核体
15°C
From Instant notes, Biochemistry, E2
Cell tracer
C7000 C68H105Cl2N3O
荧光染色的斑马鱼胚胎
A 30 µm–thick section of a zebrafish embryo stained with CellTracker CM-DiI (C7000, C7001) prior to immunohistochemical analysis. A one-dayold zebrafish embryo was immobilized and impaled in the hindbrain with a microelectrode filled with 1 mg/mL CellTracker CM-DiI in 95% ethanol. One day later, the brain was fixed, embedded, frozen in liquid nitrogen and sectioned on a cryostat. After blocking with 0.1% Triton X100 in PBS containing 2% BSA and 2% normal goat serum, the 30 µm–thick section was incubated with primary anti-glial antibody in conjunction with fluorescein goat anti–mouse IgG antibody. This section was then viewed sequentially through optical filter sets appropriate for rhodamine and fluorescein, and the resulting images were superimposed. The image was contributed by William Trevarrow, Beckman Institute, California Institute of Technology.
Sensor (3)
Fluorescence Resonance Energy Transfer
hv
FRET S
R
T
+
hv
hv’
hv
D,exD,emA,ex A,em F
D,exD,emA,ex A,em F
Probing DNA
Moleculຫໍສະໝຸດ Baidur beacons 分子信标
F
Q
Target DNA
ssDNA binding protein
Q
Denaturing reagent
QF AD
+
S-R
T
hv hv
Al3+
hv
Sensor (5)
竞争型
+
S-R
T
催化型
+
S-R
T
+
hv
+
催化剂中间体
Tracer The Fusion of Integal Membrane Protein
荧光素标记 鼠蛋白质
T1 Fluorescence
IC
hF 10-8 s
Phosphorescence ISC
10-3100 s hP
3210
Characteristics of Fluorophore
Excitation wavelength 激发波长 ex Emission wavelength 发射波长 em Extinction (absorption) coefficient 吸光系数
联系方式:
(L), 2187288(O), 2182355(H)
Fluorescent Probes
Introduction Fluorescent Sensor 荧光传感 Fluorescent Tracer 荧光示踪 FRET 共振能量转移荧光
Application
1. Radical probes 2. Ion probes 3. NIRF Fluorescence image 4. DNA sequence
5. Protein probes 6. DNA probes 7. Membrane probes 8. pH probes
Fluorescence research
Key word: Fluorescen*
Period: Jan/2005-Jun/ 2005
Data base:
➢ 美国化学会 ( ACS )数据库 ➢ WPS电子期刊全文数据库 ➢ SDOS全文数据库（上交 镜像） ➢ 英国皇家化学会（RSC) 数据库