PD-1和PD-L1在结直肠癌中的表达及其临床病理意义

PD-1和PD-L1在结直肠癌中的表达及其临床病理意义

摘要】目的：探讨程序性死亡受体１(programmed cell death-1,PD-1）与程序性死

亡配体１(programmed cell death-ligand 1,PD-L1）在结直肠癌（CRC）中的表达及

其与临床病理参数的相关性。方法：采用免疫组织化学方法检测病例组（172例

结直肠癌患者癌组织及82例对应淋巴结转移癌组织）与对照组（50例癌旁组织

及50例非肿瘤肠黏膜组织）中PD-1、PD-L1的表达情况，分析其与各临床病理特征的相关性。结果：病例组PD-1、PD-L1的表达均高于对照组，差异有统计学意

义(P<0.05)，癌细胞中PD-L1表达与年龄密切相关(P<0.01)，肿瘤浸润淋巴细胞中PD-L1表达与年龄、TNM分期以及浸润深度(P<0.05)密切相关。癌间质PD-1表达

与性别和肿瘤大小(P<0.05)密切相关。结论：结直肠癌中PD-1、PD-L1表达显著

升高，并与患者临床病理特征密切相关；二者可能成为预测结直肠癌进展和预后

的新分子标志物，阻断PD-1/PDL1信号通路有望成为结直肠癌免疫治疗的新策略。

【关键词】结直肠癌；PD-1；PD-L1；肿瘤浸润淋巴细胞（TIL）；免疫抑制

【中图分类号】R735.3 【文献标识码】A 【文章编号】2095-1752（2019）28-0018-03

Expression and clinical significance of PD-1 and PD-L1 in Carcinoma of the colon and rectum(CRC)

Han Lifang1, Wang Zhen2, Guan Ying3

1 Department of Pathology, Clifford Hospital of Guangzhou University of Traditional Chinese Medicine, Guangzhou, Guangdong 511495, China

2 Science and Educadon Section, Clifford Hospital of Guangzhou University of Traditional Chinese Medicine. Guangzhou Guangdong 511495, China

3 Department of Statistics, Southern Medical University, Guangzhou, Guangdong 511495, China

【Abstract】Objective To investigate the correlation between the expression of PD-1/PD- L1 in Carcinoma of the colon and rectum(CRC) and clinicopathologic features. Methods The study comprised tumor sections from 172 CRC patients treated w ith curative resection, 82 matched lymph nodes, 50 cases of adjacent normal tissues and

50 cases of health controls, which were evaluated for PD-1 and PD-L1 protein expression by immunohistochemistry. Results The expression of PD-1 and PD-L1 in CRC was significantly (P<0.05) higher than that in normal tissues, which were highly positively correlated with each other (P<0.001). The expression of PD-L1 in cancer nests was significantly correlated with age(P<0.01). and which in tumor infiltrating lymphocytes was correlated with age, TNM classification, and depth of

invasion(P<0.05). PD-1 expression in interstitial lymphocyte was correlated with sex

and tumor volume(P<0.05). Conclusion PD-1 and PD- L1 are overexpressed and significantlycorrelated with clinicopathologic features in CRC. PD-1/PD-L1 expression plays a critical role in the pathogenesis of human CRC, suggesting that it might be used as a new bio-marker to predict the disease progression and prognosis .

【Key words】 Carcinoma of the colon and rectum(CRC);PD-1;PD-L1;tumor infiltrating lymphocytes(TIL); Immune evasion

结直肠癌（CRC）作为最常见的恶性肿瘤之一，在全世界男性的发病率排名

第３位，病死率第４位，女性的发病率排名第２位，病死率第３位。晚期结直肠

癌(指局部晚期或远处转移），占所有结直肠癌的50%。我国结直肠癌的发病率和

病死率呈明显上升趋势，且发病年龄有所提前。而针对结直肠癌的治疗，仍然是