补肾化瘀祛痰方对雄激素致不孕大鼠卵巢局部调控因子的影响

补肾化瘀祛痰方对雄激素致不孕大鼠卵巢局部调控因子的影响

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【摘要】目的：观察补肾化瘀祛痰方（Bushen Huayu Qutan Recipe, BHQR）对雄激素致不孕大鼠（androgen sterilized rat, ASR）卵巢局部调控因子的影响。方法：选择9日龄幼年SD雌性大鼠105只，随机分为病理模型组（90只）和溶媒对照组（15只），丙酸睾丸酮诱导形成ASR。造模成功的30只大鼠随机分为中药干预组和模型对照组。中药干预组用BHQR灌胃干预，模型对照组及溶媒对照组给予蒸馏水灌胃处理，疗程30 d。疗程结束后观察大鼠性周期情况，用放射免疫方法检测血睾酮和胰岛素（insulin, INS）含量，用免疫组织化学方法检测卵巢组织抑制素（inhibin, INH）、胰岛素样生长因子Ⅰ(insulin like growth factorⅠ, IGFⅠ)和血管内皮生长因子（vascular endothelial growth factor, VEGF）的表达。结果：BHQR 不仅能明显降低大鼠血INS水平（P0.05），还能显著降低INH、IGF Ⅰ和VEGF在卵巢局部的表达（P0.01）。大鼠排卵恢复率为66.67％。结论：BHQR可能通过改善胰岛素抵抗，降低INH、IGFⅠ及VEGF

水平，改善卵巢内部环境，多途径、多靶点促使卵巢功能恢复，促进卵泡生长发育，诱发排卵。

【关键词】补肾化瘀祛痰方; 不育; 抑制素类; 胰岛素样生长因子Ⅰ; 血管内皮生长因子类; 大鼠

Objective: To explore the effects of Bushen Huayu Qutan Recipe (BHQR), a compound traditional Chinese herbal medicine, on local ovarian factors in androgen sterilized rats (ASR).Methods: One hundred and five 9day old SD rats were randomly divided into normal control group (15 rats) and ASR group (90 rats). ASR was induced by subcutaneous injection of testosterone propionate, and the rat model of ASR was established successfully in 30 rats in the ASR group. Thirty ASR rats were randomly divided into untreated group and BHQR treated group. There were 15 rats in each group. The rats in BHQR treated group were intragastrically administered with BHQR. The rats in the normal control and untreated groups were intragastrically administered with distilled water. They were all treated for 30 days. The levels of serum testosterone and insulin (INS) were measured by radioimmunoassay. The inhibin (INH) level, and the expressions of insulin like growth factorⅠ(IGFⅠ) and vascular endothelial growth factor (VEGF) in ovary were determined by immunohistochemical method.Results: None of the ASR ovulated and all the rats in the normal control group ovulated.

The rate of ovulation in BHQR treated group was 66.67%. The serum concentrations of testosterone and INS in the untreated group were significantly higher than those in the normal control group (P0.05), and the serum concentrations of testosterone and INS in BHQR treated group were decreased as compared with the untreated group (P0.05). The levels of ovarian INH, IGFⅠand VEGF in the untreated group were significantly higher than those in the normal control group (P0.01), and the levels of ovarian INH, IGFⅠand VEGF in BHQR treated group were decreased as compared with the untreated group (P0.01).

Conclusion: BHQR is an effective recipe in promoting ovulation, and the effects of BHQR on balancing the internal environment of ovary may be due to the reduction of serum INS level and decrease in the expressions of ovarian INH, IGFⅠand VEGF.

Keywords: Bushen Huayu Qutan Recipe; infertility; inhibins; insulin like growth factorⅠ; vascular endothelial growth factors; rats 多囊卵巢综合征（polycystic ovary syndrome, PCOS），又称Stein Leventhal综合征，是一种发病多因性、临床表现多态性疾病，以长期无排卵和持续高雄激素为主要特征［1］。目前关于PCOS的发病机制学说众多，但确切病因仍不明确，且缺乏理想的治疗方法。西医主要有手术和药物等治疗方法，但由于手术创伤较大，而西药长