BIQ-Analyzer 演示过程

Description: When the BiQ Analyzer program is properly installed you can start BiQ Analyzer simply by clicking on the "start BiQ_Analyzer.bat"

batch file (Windows) or by running the shell script

"unix_start_BiQ_Analyzer" (Unix/Linux/MacOS). If you have any problems, please consult the Installation section and the FAQ / Troubleshooting

section.

Description: This is the main screen of BiQ Analyzer. Its main elements are the status indicator on the top left, the text box for the genomic sequence at the top, some parameter settings on the top right, an empty space for the input sequences on the left and an empty space for the multiple sequence alignment on the right. In addition - and most importantly - there is a message box at the bottom right, which will give you hints on how to proceed in each step. Please read these messages carefully - they will help you to understand how the program works. We will now proceed as suggested: first paste the genomic sequence into the text box at the top and then press the "Next" button. Importantly, the genomic sequence must not contain primers and must be unconverted!

Description: The program requests us now to select the raw sequence files that were bisulphite converted and sequenced. These files must be in FASTA format. They should have the same orientation as the genomic sequence and it is helpful if they don't contain any primers (although both can be adjusted for later on). Each file must contain exactly one sequence, it is not possible to import multiple sequences from one file. Furthermore, all sequences must be located in the same directory (if all this sounds complicated, don't worry: just select the sequences as they come out of the sequencer's software and, most likely, it will work!). You can find the example sequences for this guided tour in the "Demo_data" subdirectory of your BiQ Analyzer installation directory.

If you have trouble to select multiple files, here are some hints for Windows users: to select all sequences from one directory, please select one and then press . To select sequences individually, keep or pressed and click at the files with the mouse. Finally, press the "Open" button to start the analysis.

Description: If everything works fine, you will now see two thing happen. Firstly, the sequences that you selected will appear on the left. Secondly, there will briefly appear a dialog box indicating the the program is currently busy with an alignment calculation. Please be patient until this is finished. For this exemplary analysis it should not take more than 30 seconds but if you use a high number of sequences (>20) the waiting time may be significantly longer.