蛋白质Geneontology-KEGG分析软件David使用方法介绍

DAVID Gene Functional classification
The Functional Classification Tool generates a gene-to-gene similarity matrix based shared functional annotation using over 75,000 terms from 14 functional annotation sources. The novel clustering algorithms classifies highly related genes into functionally related groups.
Gene List Manager
DAVID Analytic Modules
Any given gene is associating with a set of annotationterms. If genes share similar set of those terms, they are most likely involved in similar biologicalmechanisms. The algorithm adopts kappa statistics to quantitatively measure the degree of the agreement how genes share the similar annotation terms. Kappa result ranges from 0 to 1. The higher the value of Kappa, the stronger the agreement. Kappa more than 0.7 typically indicates that agreement of two genes are strong. Kappa values greater
2. before proceeding to analysis with other more comprehensive analytic tools, investigators can quickly glance at the gene names to further gain insight about their study and源自文库to answer questions.
than 0.9 are considered excellent.
Parameter Panel
Gene Clusters Identified by
DAVID
User’s gene IDs & Names
1
3 Percentage,e.g.44/163
3 Genes from the list
DAVID
Database for Annotation, Visualization and Integrated Discovery
DAVID provides a comprehensive set of functional annotation tools for investigators to understand biological meaning behind large list of genes
involved in this annotation category
2 View and select
annotation categories of your interests
3 Single chart report
only for this Annotation categories
Table report for all Selected annotation categories
DAVID Gene Name Batch Viewer
1. The gene name batch viewer is able to quickly attach meaning to a list of gene IDs by rapidly translating them into their corresponding gene names.
Identify enriched biological themes, particularly GO terms Discover enriched functional-related gene groups Cluster redundant annotation terms Visualize genes on BioCarta & KEGG pathway maps Display related many-genes-to-many-terms on 2-D view. Search for other functionally related genes not in the list List interacting proteins Explore gene names in batch Link gene-disease associations Highlight protein functional domains and motifs Redirect to related literatures Convert gene identifiers from one type to another.
DAVID Gene ID Conversion Tool (DGCT)
If a significant portion (>20%) of input gene IDs fail to bemapped to an internal DAVID ID, a specially designed module, the DAVID Gene ID Conversion Tool, will start up to help map such IDs.
Linear or redundant chart report of annotation terms for all selected annotation categories above
Clustered or non-redundant chart report of annotation terms for all selected annotation categories above