江苏淮北地区小麦品种资源遗传多样性的SSR分析

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麦类作物学报 2014,34(5):628-

634Journal of Triticeae Crops 

doi:10.7606/j

.issn.1009-1041.2014.05.09网络出版时间:2014-5-

12网络出版地址:http

://www.cnki.net/kcms/doi/10.7606/j.issn.1009-1041.2014.05.09.html江苏淮北地区小麦品种资源遗传多样性的SSR分析

收稿日期:2013-12-22 修回日期:2014-02-

13基金项目:江苏省农业科技自主创新项目(CX(13)2022);江苏省科技支撑项目(BE2013439);淮安市农科院院长基金项目;淮安市产学研合作促进计划项目(HAC201118

)第一作者E-mail:yang

zibo1986@126.com通讯作者:顾正中(E-mail:hynksg

zz@163.com)杨子博1,顾正中1,周羊梅1,王安邦1,李立群2,李学军2

(1.江苏徐淮地区淮阴农业科学研究所,江苏淮安223001;2.西北农林科技大学农学院,陕西杨凌712100

)摘 要:为明确江苏淮北地区小麦品种资源的遗传基础,选用31对SSR标记对107份近年来淮北地区所育小麦材料进行了遗传多样性分析,共检测出170个等位变异,单个引物的等位变异数为3~8个,平均为5.48个;位点多态性信息含量变幅为0.176~0.791,平均为0.543;3个基因组的平均等位变异丰富度及遗传多样性指数均为D>B>A;江苏淮北5个地区中以徐州小麦材料的平均遗传多样性指数最高(0.613),以淮安小麦材料与江苏淮北另外4个地区的平均遗传距离最小(0.282)。聚类结果表明,品种间遗传距离变幅为0~0.935,平均为0.586,除淮麦20与华瑞0049外,SSR标记能将其他供试材料相互区分开;所有供试材料被聚为3大类,聚类结果与品种(系)的系谱来源比较吻合。

关键词:小麦;遗传多样性;SSR标记

中图分类号:S512.1;S330 文献标识码:A 文章编号:1009-1041(2014)05-0628-

07Analysis of Genetic Diversity among Wheat Cultivars fromHuaibei Region of Jiangsu Province using 

SSR MarkersYANG Zibo1,GU Zhengzhong1,ZHOU Yangmei 1,WANG Anbang1,LI Liqun2,LI Xuej

un2

(1.Huaiyin Institute of Agricultural Sciences in Xuhuai Region of Jiangsu Province,Huai’an,Jiang

su 223001,China;2.College of Agronomy,Northwest A&F University,Yangling

,Shaanxi 712100,China)Abstract:Thirty-one SSR primers located on different wheat chromosome were used to analyze the ge-netic diversity of 107wheat cultivars from Huaibei region of Jiangsu province.Totally 

170variationswere detected among all tested materials,with allele number per locus ranged from 3to 8and a meanallele number of 5.48.The range of polymorphic information content was from 0.176to 0.791,withan average of 0.543.Both the average genetic richness and the genetic diversity indexes for three ge-nomes were as D>B>A.The genetic diversity index of Xuzhou wheat cultivars were the highest a-mong the five Huaibei regions(0.613),and the average genetic distance between Huaian wheat culti-vars and wheat cultivars from another four regions were the smallest(0.282).Cluster analy

sisshowed the genetic distance(GD)variation range was 0~0.935,with an average of 0.586.Except forHuaimai 20and Huarui 0049,all the other cultivars could be distinguished by 

SSR markers.All culti-vars were clustered into 3groups,which is generally consistent with their pedigrees.Key words:Wheat cultivar;Genetic diversity;SSR marker 长期以来,

小麦育种工作者的注意力主要集中于对产量、品质、抗性等主要目标性状的选择

上,在育种过程中又大量使用少数骨干亲本,致使所育成的小麦品种遗传基础狭窄,遗传多样性水

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