植物免疫系统

Plant immune system activation by pathogen effectors that generate modified self molecular patterns
Co-evolution of host R genes and the pathogen effector complement
定位克隆技术是根据目标基因在染色体上的位置进行基因克隆的一种方法,目标基因精确定位在染色体特定位置之后, 用目标基因两侧紧密连锁的标记筛选含有大的插入片段的基因组文库(如BCA和YA)C,通过染色体步行 (ChormosomeWalkign)筛选到含有目标基因的克隆,最后通过遗传转化和功能互补实验进行验证。其核心任务是染 色体步行,如果能找到与目标基因很近的标记,以至于二者之间的距离小于基因组文库中克隆的平均插入片段大小,就可 以直接筛选到含有目标基因的克隆,最终得到候选基因,这种策略称为染色体登陆(ChromosomeLandign)。该方法的 关键是需要高密度的分子标记图谱和找到与目标基因紧密连锁的分子标记。
A zigzag model illustrates the quantitative output of the plant immune system.
MAMPS or PAMPs：microbial- or pathogen-associated molecular patterns PTI:PAMP-triggered immunity PRRs: pattern recognition receptors ETS:effector-triggered susceptibility ETI: effector-triggered immunity HR: hypersensitive cell death response
1.图位克隆法 2.转座子标签法 3.同源序列法
转座子标签法技术克隆基因的主要步骤是:①采取农杆菌介导等方法把转座子导人 目标生物体;②转座子在目标生物体内的初步定位;③转座子插入突变的鉴定和分离; ④利用一些带转座子及报告基因的重组质粒来检测转座子在目标生物体内的活动性;⑤ 对转座子插入引起的突变体,利用转座子序列作探针,分离克隆目的基因。
The plant immune system
A zigzag model illustrates the quantitative output of the plant immune system.
MAMPS or PAMPs：microbial- or pathogen-associated molecular patterns PTI:PAMP-triggered immunity PRRs: pattern recognition receptors ETS:effector-triggered susceptibility ETI: effector-triggered immunity HR: hypersensitive cell death response
A zigzag model illustrates the quantitative output of the plant immune system.
MAMPS or PAMPs：microbial- or pathogen-associated molecular patterns PTI:PAMP-triggered immunity PRRs: pattern recognition receptors ETS:effector-triggered susceptibility ETI: effector-triggered immunity HR: hypersensitive cell death response
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