黑果枸杞花青素类型、含量及结构分析研究

食品研究与开发

F ood Research And Development

圆园18年7月第39卷第13期

DOI：10.3969/j.issn.1005-6521.2018.13.026

黑果枸杞花青素类型、含量及结构分析研究

杭园园1，李悦1，王朝警1，束彤2，陈虎1，王茜2，王树林1，2，*

（1.青海大学农牧学院，青海西宁810016；2.青海大学省部共建三江源生态与高原农牧业国家重点实

验室，青海西宁810016）

摘要：研究高效液相色谱（high performance liquid chromatography，HPLC）检测黑果枸杞中花青素含量的最佳方法，采用高效液相色谱-串联质谱（high performance liquid chromatography-tandem mass spectrometry，HPLC-MS）分析黑枸杞中的花色苷结构。以超声波辅助提取获得的花青素粉末作为样品，对影响检测结果的样品水解时间，液相流速等因素进行研究，并采用高效液相色谱-串联质谱检测花青素。确定水解液比例为乙醇∶水∶盐酸=3∶2∶1（体积比），水解液中HCl浓度为3.0mol/L，水解时间1h，流速为0.7mL/min时，液相检测效果较好。结果表明，黑果枸杞中飞燕草素、矢车菊素、矮牵牛素和锦葵素的浓度分别为2.32、15.44、35.18、2.63μg/mL。通过试验确定高效液相色谱法的最佳检测条件，确定黑果枸杞花青素的含量；并对黑果枸杞花青素进行一定的结构分析，得到较好的结果，并检测出14种花色苷成分。

关键词：黑果枸杞；花青素；类型；结构；高效液相色谱；高效液相色谱-串联质谱

Analyze of Types，Contents and Structure from Lycium ruthenicum Murr.Anthocyanidins HANG Yuan-yuan1，LI Yue1，WANG Zhao-jing1，SHU Tong2，CHEN Hu1，WANG Qian2，WANG Shu-lin1，2,*（1.Academy of Agriculture and Animal Husbandry，Qinghai University，Xining810016，Qinghai，China；

2.State Key Laboratory of Plateau Ecology and Agriculture，Qinghai University，Xining810016，Qinghai，

China）

Abstract：A best method of analyzing anthocyanidins of Lycium ruthenicum Murr.was studied by using high performance liquid chromatography（HPLC）and study on structure of anthocyanin in Lycium ruthenicum Murr. by high performance liquid chromatography-tandem mass spectrometry（HPLC-MS）.The HPLC-MS was used to analysis kind and content of anthocyanidins from Lycium ruthenicum Murr..The anthocyanidins was extracted by ultrasonic assistance extraction using powder of Lycium ruthenicum Murr.dissolved The conditions of samples hydrolyzed and detection by HPLC was studied using the chromatogram quality as index.The results showed that anthocyanidin of Lycium ruthenicum Murr.was hydrolyzed in hydrolysate solution comprised of alcohol solu-tion，distilled water and hydrochloric acid solution according to the volum ratio of3∶2∶1.The concentration of HCl in hydrolysate solution was3.0mol/L.It was hydrolyzed in boiling water for1h.The flow speed of liquid phase was0.7mL/min.There were good results of HPLC detection according to the conditions that had been se-lected.The anthocyanins in Lycium ruthenicum Murr.had been detected by the HPLC.The result showed that the content of delphinidin，cyanidin，petunidin，and malvidin was2.32，15.44，35.18，2.63μg/mL in Lycium ruthenicum Murr.respectively.The HPLC-MS method was used to analyze the components of anthocyanidins in Lycium ruthenicum Murr..The optimum conditions for the determination of anthocyanin were determined by ex-periment.The results showed that the anthocyanin content of Lycium ruthenicum Murr.was determined and the results were obtained and detected14kinds of anthocyanin components.

Key words：Lycium ruthenicum Murr.；anthocyanidins；types；structure；high performance liquid chromatogra-phy（HPLC）；high performance liquid chromatography-tandem mass spectrometry（HPLC-MS）

作者简介：杭园园（1994—），女（汉），硕士，研究方向：食品工程。

*通信作者：王树林（1970—），男（汉），教授，博士，研究方向：青藏高原特色食品资源开发。

标准与检测143