小鼠早期胚胎发育

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Development 139, 3-14, 2012
Two possible mechanisms for the generation of multiple cell types and polarity
Development 139, 3-14, 2012
Mouse trophectoderm distinguish from inner cell mass regulated by Hippo signaling pathway
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Development 139, 3-14, 2012
Early mouse development
E3.5 ICM
E4.5
‘‘Salt and Pepper’’ expression of Nanog and Gata6 in the ICM of E3.5 blastocysts
Dev Cell 10, 615-624, 2006
Dev Cell 15, 509-520, 2008
Cell lineages in early mouse embryo
Cell lineage in early mouse embryo
Epiblast
Ectoderm
Mesendoderm Definitive endoderm
Epidermis
Development 139, 3-14, 2012
Mouse embryo development from fertilization to gastrulation
Development 139, 3-14, 2012
Early mouse embryo development and lineage segregation
Fossat, N., et al. (2012) Cell Cycle 11: 26-32
“It is not birth, marriage, or death, but gastrulation, which is truly the most important time in your life.” Lewis Wolpert, 1986
Lateral plate mesoderm
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Paraxial mesoderm
Neuroectoderm
Definitive endoderm
Axial mesoderm
The markers of germ layers
Epiblast Nodal, T, Wnt3, Gsc Mesendoderm
Emerging asymmetry and establishment of anteriorposterior polarity in the E5.75 mouse embryo
Axis of Anterior-Posterior Axis of Proximal & Distal
Cell movements in early mouse embryos
FGF5, Nodal
Ectoderm
Neural Sox1 Pax6 Six3 Nestin Epidermis Ck5 Ck8 Ck18
Definitive endoderm
Mesoderm
Sox17 Gata4 Gata6
Foxa2
Nkx2.5 Mixl1 Flk1
Mesp1
Zo-1
Fate-mapping of the distal cap epiblast by carbocyanine dye labeling
CNS
Mesoderm
T
Ectoderm
Mesendoderm
E7.0
Mesendoderm markers
Bra
E7.5
Wnt3
Gene expression and regionalized allocation of tissue progenitors
Extraembryonic mesoderm
E7.5 E8.5
Axis of Dorsal-Ventral
Myocardin
Cell lineages in early mouse embryo
Embryo culture system for time-lapse movie acquisition of node morphogenesis
Distal VE to AVE
Epiblast to posterior
Extraembryonic ectoderm to posterior epiblast
Epiblast to posterior
Movement of Otx2-positive cells from DVE to AVE
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Dev Cell 16, 398-410, 2009
Cell position-dependent differentiation mediated by Hippo signaling
Development 139, 3-14, 2012
Mouse embryo development from fertilization to gastrulation
Primitive streak formation
VE: visceral endoderm AVE: anterior VE DVE: distal VE
PS: Primitive Streak
EMT at mouse primitive streak
EMT at mouse primitive streak
Nat Cell Biol 13, 743-752, 2011
Origins of the dorsal and anterior visceral endoderm
Development 139, 3-14, 2012
Signaling pathways involved in A-P axis determination
Dev Cell 10, 451-459, 2006
Two types of Lefty1+ cell in mouse embryos between E3.5 and E4.5
Nat Cell Biol 13, 743-752, 2011
Distinct origins of DVE and AVE
Segregation and relocation of epiblast and PrE cells
Development 139, 3-14, 2012
Mouse embryo development from fertilization to gastrulation
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Development 139, 3-14, 2012
E6.5
Dye injection
The distal cap epiblast cells migrate to the anterior neuroectoderm
M ag a zi ne
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ht yol k Epi bl ast nd
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pxm l m ht
Yol k cel l s
in vitro embryo culture
GFP knock-in tracing
Dev Cell 13, 884-896, 2007
Time-lapse images of node morphogenesis
Noto-
Dev Cell 13, 884-896, 2007
The displacement model of endoderm morphogenesis in the mouse gastrula
Trophectoderm
Epiblast
Parietal endoderm
Visceral endoderm
Mesendoderm
Ectoderm
Definitive endoderm
Mesoderm
Epidermis
CNS
Mouse embryo development from fertilization to gastrulation
小鼠早期胚胎发育
景乃禾
中国科学院上海生物化学与细胞生物学研究所
体轴形成和原肠运动
Axis formation & Gastrulation
小鼠早期胚胎发育
Early mouse development
Preimplantation
Early mouse development
Postimplantation
The dispersal model of endoderm morphogenesis in the mouse gastrula
Visceral endoderm-derived cells are incorporated into the gut tube
12–18 somite embryos revealed the presence of Afp-GFP+ cells in the gut tube
This was not new expression of the transgene, as GFP mRNA could only be detected in the yolk sac at this stage
These visceral endoderm derived cells continue to divide and are eventually incorporated into the epithelium of the gut tube
Nervous system 3.5dpc ICM Skin and hair
4.5dpc early epiblast
Heart
……
Muscle
liver
……
Lungs 5.5dpc late epiblast 6.5dpc
Primitive streak
Rapid proliferation during gastrulation: dramatic signaling and gene expression changes
(early) Epiblast
VE: visceral endoderm AVE: anterior VE DVE: distal VE
(late) Epiblast
Anterior neuroectoderm
Cell lineages in early mouse embryo
Morula
Inner Cell Mass Primitive endoderm
5.5 dpc 5.75 dpc
wenku.baidu.com
6.0 dpc
6.25 dpc
Otx2 VEcis-lacZ
Otx2 KI-lacZ
DVE: distal visceral endoderm; AVE: anterior visceral endoderm
Asymmetric expression of Lefty1 transgenes in peri-implantation mouse embryos
Epiblast Primitve Streak Nascent Mesoderm
Cell Adhesion & Migration 4:3, 447-457
Mouse gastrulation and germ layer formation
Cell 132, 661–680, 2008
Late mouse development