生物基尼龙

30 ℃ baffled flasks 200 mL medium 8 h.
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2015-1-5
problem
global warming ozone depletion acid rain smog
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Without doubt, a highly promising bio-block for polyamides is 1,5-diaminopentane (cadaverine, diaminopentane), a fivecarbondiamine. In this work, we describe a sustainable value chain, extending from renewable resources to a novel, bio-based polyamide PA5.10. The development integrated engineering of C. glutamicumat the cellular and the process level to ensure fermentative supply of the polyamide building block, diaminopentane.
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Result
1.Expression of lysine decarboxylase in the lysine hyperproducer C. glutamicum LYS-12 provides diaminopentane as major product
戊二胺产量
Lys高产
N-乙酰-戊二胺产量
From zero to hero - Production of bio-based nylon from renewable resources using engineered Corynebacterium glutamicum
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菌体OD
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5.Bio-nylon PA5.10 reveals excellent material properties as compared to those of the petrochemical polyamides PA6 and PA6.6.
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Materials and Methods
C. glutamicum LYS-12 Escherichia coli strains DH5α and NM522 plasmids pClik int sacB and pTc
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3.Metabolic engineering of the exporters for lysine and diaminopentane increases product yield
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Fermenter
Cells were harvested by centrifugation (8800g, 5 min, 4℃)
Feeding was started at a glucose concentration of10 g/L and adjusted such that the glucose concentration in the fermenter remained above 10 g/ L washed once with fermenter sterile 5%NaCl, resuspended in 30 mL of 5% NaCl
赖氨酸产量
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2.Deletion of the N-acetyl transferase NCgl1469 eliminates N-acetyldiaminopentane as by-product.
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DAP-15 DAP-13 DAP-14 DAP-16
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Introduction Materials and Methods Results Discussion
第一PPT模板网 WWW.1PPT.COM
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Introduction
Polyamide (PA), commonly known as nylon, is a polymer with a myriad of pharmaceutical and industrial applications. Chemically, the polymer backbone is composed of repetitive units of diamines and dicarboxylic acids that contain different numbers of carbon atoms, imparting a variety of material properties.
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7. Reinforcement of the bio-nylon PA5.10 with glassfibers increases mechanical stability.
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Materials and Methods
strain
shakeflasks
fermenter
test method
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4.The designed producer C. glutamicum DAP-16 accumulates 88 g /L diaminopentane under industrial fermentation conditions.
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Discussion
1.The synthetic producer C. glutamicum DAP-16 enables good diaminopentane production from renewable resources 2.From the viewpoint of material performance, bio-based PA5.10 might replace conventional petrochemical nylons 3.Bio-based polyamide PA5.10–a pioneering bio-based polymer with advanced material properties