国外微生物学基础实验(三)-英文版
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Blood Agar = very dark red McConkeys = lighter, purplish-pink Mannitol Salt -= orangish-pink
MacConkey's (already prepared)
MacConkey’s is both a selective & differential media.
• You may turn in your dirty copy of Lab #3 to save for use next week.
• Lab Report #2 and #3 due next week, as week, as well as Pre-lab #4.
Laboratory 3
Getting Ready for Today’s Lab
• Grab your lab check-in card and the dirty copies of Lab #2 and #3 that we saved from last week.
• Grab your plastic box of supplies.
Blood agar
Most specimens received in a clinical microbiology lab plated onto Blood Agar, since it supports the growth of most organisms. Blood agar contains
Members of the family Enterobacteriaceae (Gram negative bacilli) are the most frequently encountered bacteria isolated from many types of clinical specimens. They are most commonly lactose fermenters.
Differential Staining
G
s
u
பைடு நூலகம்
e
Three Main Things We are Doing Today
I. Looking at Results from Last Weeks Cultures
II. Learning to use an Inoculation Loop for Streak Plate Method
• Put on your lab coat and have your goggles, lab card and dirty copies of lab exercises/reports on the bench next to you.
• Plug in and turn on your microincinerator.
III. Differential Stains
I. Looking at Results from Last Weeks Cultures
Other Types of Media: McConkey’s, Blood Agar & Mannitol Salt
Look at the plates on your lab bench that are red/pink In color.
Gamma hemolysis: No effect on the red cells.
Blood agar is usually inoculated from a patient’s throat swab.
• TURN IN: No lab report or Pre-lab due this week.
End of Today’s Lab
• After you have cleaned your lab bench, copy your Lab #2 and #3 material on to a clean copy to take home. I must see you throw out your dirty copy of Lab #2.
- Bacteria that use lactose (a type of sugar) for food, produce acidic metabolites which causes the pH indicator neutral red to turn red.
- These “lactose fermenters” will grow in red colonies while non-lactose fermenters will be colorless and clear.
1. What Does Selective Mean? MacConkey’s is selective media because it inhibits the growth of some organisms [Gram positive bacteria].
2. What Does Differential Mean? Because “neutral red” has been added to the media, it is also differential.
5% sheep blood.
This media is differential because:
Certain bacteria produce enzymes (hemolysins…hemo-lice-ins) that act on the red cells to produce either:
Beta hemolysis: Enzymes lyse the blood cells completely, producing a clear area around the colony.
Alpha hemolysis: Incomplete hemolysis produces a greenish discoloration around the colony.
MacConkey's (already prepared)
MacConkey’s is both a selective & differential media.
• You may turn in your dirty copy of Lab #3 to save for use next week.
• Lab Report #2 and #3 due next week, as week, as well as Pre-lab #4.
Laboratory 3
Getting Ready for Today’s Lab
• Grab your lab check-in card and the dirty copies of Lab #2 and #3 that we saved from last week.
• Grab your plastic box of supplies.
Blood agar
Most specimens received in a clinical microbiology lab plated onto Blood Agar, since it supports the growth of most organisms. Blood agar contains
Members of the family Enterobacteriaceae (Gram negative bacilli) are the most frequently encountered bacteria isolated from many types of clinical specimens. They are most commonly lactose fermenters.
Differential Staining
G
s
u
பைடு நூலகம்
e
Three Main Things We are Doing Today
I. Looking at Results from Last Weeks Cultures
II. Learning to use an Inoculation Loop for Streak Plate Method
• Put on your lab coat and have your goggles, lab card and dirty copies of lab exercises/reports on the bench next to you.
• Plug in and turn on your microincinerator.
III. Differential Stains
I. Looking at Results from Last Weeks Cultures
Other Types of Media: McConkey’s, Blood Agar & Mannitol Salt
Look at the plates on your lab bench that are red/pink In color.
Gamma hemolysis: No effect on the red cells.
Blood agar is usually inoculated from a patient’s throat swab.
• TURN IN: No lab report or Pre-lab due this week.
End of Today’s Lab
• After you have cleaned your lab bench, copy your Lab #2 and #3 material on to a clean copy to take home. I must see you throw out your dirty copy of Lab #2.
- Bacteria that use lactose (a type of sugar) for food, produce acidic metabolites which causes the pH indicator neutral red to turn red.
- These “lactose fermenters” will grow in red colonies while non-lactose fermenters will be colorless and clear.
1. What Does Selective Mean? MacConkey’s is selective media because it inhibits the growth of some organisms [Gram positive bacteria].
2. What Does Differential Mean? Because “neutral red” has been added to the media, it is also differential.
5% sheep blood.
This media is differential because:
Certain bacteria produce enzymes (hemolysins…hemo-lice-ins) that act on the red cells to produce either:
Beta hemolysis: Enzymes lyse the blood cells completely, producing a clear area around the colony.
Alpha hemolysis: Incomplete hemolysis produces a greenish discoloration around the colony.