黄芪中总黄酮的含量测定方法_图文(精)

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黄芩中总黄酮的含量测定 To determine the content of total flavonoids in Radix Scutellariae

班级 08级 2班

专业药学

姓名崔立靖

同组人陆晓娅关雪李娟张栋材

摘要目的:黄芩中总黄酮含量的测定。方法:采用紫外分光光度法测定黄芩中总黄酮的含量。结果:黄芩中总黄酮含量是 0.034mg/ml。

Abstract Objective :To determine the content of total flavonoids in Radix Scutellariae.

Methods :Ultraviolet spectrophotometry was used to To determine the content of total flavonoids content.

Results :Total flavonoids content of Radix Scutellariae reached 0.034mg/ml。

关键词黄芩;总黄酮;紫外分光光度法

Key WorldRadix Scutellariae; Total flavonoids ; uv-spectrophotometry

目录

要 ........................................................................................................................................... .. (1)

Abstract ................................................................................................................................. ............ 1关键

词 ...........................................................................................................................................

.... 1 Key

World .................................................................................................................................... ..... 1目

录 ........................................................................................................................................... .. (2)

1 绪

论 . ......................................................................................................................................... (3)

2 黄芩中总黄酮含量

测 . ................................................................................................................ 6 2.1仪器与试剂 . ........................................................................................................................ 6 2.1.1实验仪器 . ................................................................................................................. 6 2.1.2实验药品 . ................................................................................................................. 6 2.2试验方法 . ............................................................................................................................

6 2.2.1总黄酮成分提取 . .....................................................................................................

6 2.2.2定量实验总黄酮的含量测定 . .................................................................................

6 2.2.2.1线性关系考察 . (6)

2.2.2.2提取物含量的测定 . ...................................................................................... 7 2.3方法学检验 . ........................................................................................................................

7 2.3.1精密度实验 . .............................................................................................................

7 2.3.2稳定性考察 . .............................................................................................................

7 2.4黄芩根及粉末的显微图

像 . ................................................................................................ 7 2.4.1黄芩根的显微图像 . . (7)

2.4.2黄芩粉末的纤维图

像 . (7)

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