TK基因可编码胸苷激酶

Transfection 转染of eukaryotic 真核cells is the
acquisition 获得of new genetic markers by
incorporation 混合of added DNA.
Transforming principle 转化原理is DNA that is taken
June 24, 2020
Genetic Engineering
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Evidence of DNA is the genetic material of bacteria
June 24, 2020
Genetic Engineering
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DNA是细菌的遗传物质证据
June 24, 2020
Genetic Engineering
Summarize
DNA is the genetic material Structure and characteristics of DNA Nucleic acids hybridize by base pairing Mutations change the sequence of DNA Genetic information can be provided by DNA or RNA The interrupted gene Organization and characteristics of interrupted genes The characteristics of exons and introns Genes can be isolated by the conservation of exons Some DNA sequences code for more than one protein
2.通过转染的方式，能够将外源DNA转入至真核细胞 内，使其获得一个新的基因标记。
3.转化的原理是：携带有新DNA的菌体在表达过程中
会产生DNA所编码的蛋白，从而改变原有受体细胞的
表达特性，形成一个新的个体。
June 24, 2020
Genetic Engineering
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DNA is the genetic material of bacteria
June 24, 2020
Genetic Engineering
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1.2 DNA 是遗传物质
DNA 是细菌的遗传物质 DNA 是病毒的遗传物质 DNA 是动物细胞的遗传物质
June 24, 2020
Genetic Engineering
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DNA is the genetic material of bacteria
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DNA is the genetic material of viruses
Summary
Avirulent mutants 无效突变of a virus have lost the
capacity 能力to infect a host cell productively有效的,
that is, to make more virus.
June 24, 2020
Genetic Engineering
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本章主要内容
DNA是遗传物质 DNA结构和特点 通过碱基配对进行核酸杂交 DNA的突变 遗传信息可由DNA或RNA提供 断裂基因 断裂基因的组成和结构 外显子和内含子特点 通过外显子的特点可分离基因 一些DNA序列可编码一种以上蛋白
sequence of the DNA, the RNA is in turn used to direct production of a protein.
June 24, 2020
Genetic Engineering
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1.2 DNA is the genetic material
DNA is the genetic material of bacteria DNA is the genetic material of viruses DNA is the genetic material of animal cells
Background 背景
肺炎链球菌：可引起肺炎，其荚膜多糖成分可以引发毒性。其有多种变异 及亚型，其中S（smooth, S ）亚型的荚膜具有多糖成分，而R（rough, R） 亚型由于荚膜当中缺乏多糖成分所以不会导致毒性。
The bacterium Pneumococcus 肺炎链球菌属 kills mice by causing pneumonia肺炎. These bacteria have a smooth type (S) and a rough type (R). The S type can kill the mice, but R type can not.
produces another nucleic acid, RNA. The DNA has two strands of nucleic acid, and
the RNA has only one strand. The sequence of the RNA is determined by the
up by a bacterium and whose expression then
changes the properties 性质of the recipient cell.受体
细胞 June 24, 2020
Genetic Engineering
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DNA 是细菌的遗传物质
小结
1.病毒的无效突变能够有效使其丧失感染宿主细胞的 能力，但不会影响其在宿主细胞复制产生病毒。
June 24, 2020
Βιβλιοθήκη Baidu
Genetic Engineering
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1.1 Introduction引言
June 24, 2020
Genetic Engineering
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1.1 Introduction引言
Figure 1.2 shows the basic theme of this chapter. A gene is a sequence of DNA that
Key concepts
Bacterial transformation provided the