苦杏仁甙检测方法

西安源森生物科技有限公司苦杏仁甙检测方法

1. Instrument 设备

a HPLC-9a (Shimadzu)

b Sartorius electroni

c balance (Germany) 电子称

c. KQ-----model 100, Washing device of ultrasonic wave 100-KQ 超声波洗涤设备

2. Condition 条件

Chromatographic Column 色谱柱ќROM-150 ODS-c18(4.6mm×150mm, 5ц)

Mobile Phase: Acetonitrile:Water：Acetic acid =20∶80：0.1

流动相：乙腈：水：醋酸=20∶80：0.1（V/V/V）

Flow Rate: 1.0mL/min 流速

Wave Length: 220nm 波长

Column: room temperature 测柱：室温

3.Standard solution 标准溶液

Transfer about 8 mg amygdalin standard(Sigma company U.S.A.), accurately weighed, to a 25mL volumetric flask, add the right amount water to dissolve, Ultrasonic wave is shaken for 5 minutes till all dissolve in the water. Then add water to scale mark, it is obtained.

精确称量8mg（美国sigma公司）标准苦杏仁甙，放入25ml 的量杯，加适量水溶解，用超声波震动直至全溶。然后将水加至标度处，获得标准溶液。

4.Testing solution 测试溶液

Transfer 8mg amygdalin powder, accurately weighed, to a 25ml volumetric flask

, add the right amount water to dissolve, Ultrasonic wave is shaken for 5 minutes till all dissolve in the water, Then add water to scale mark, shake evenly, it is obtained.

精确称量8mg苦杏仁甙粉末，放入25ml的量杯中，加入适当水溶解，用超声波摇动直至溶解。然后加入水至标度处，均匀摇晃，获得测试溶液。

5. Procedure 过程

Separately inject equal volumes (about 20 uL) of the Standard preparation and the Assay preparation into the chromatograph, record the chromatograms, and measure the responses for the major peaks.

A1 W2

X%=----- ×---- ×100%

A2 W1

In which is A1 the assay sample , A2 is the standard peak area, W2 is the concentration of standard, W1 is the concentration of assay sample.

将等量的（大约20 UL）标准溶液和测试溶液（大约20 UL）分别注入色谱仪，记录两个色相图谱测量两个主峰的反应变化。

A1 W2

X%=----- ×---- ×100%

A2 W1

上式中，A1指测试样的峰面积，A2是标准溶液的峰面积，W2指标准溶液的浓度，W1指测试样的浓度。